Probiotic composition and preparation method and application thereof
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A composition and technology of probiotics, applied in the field of probiotics, can solve the problems of no obvious effect and ineffective effect of constipation treatment, and achieve the effect of alleviating constipation, taking into account product efficacy and cost control
Active Publication Date: 2021-07-23
SIRIO PHARMA CO LTD
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[0004] Martoni et al. for Lactobacillus acidophilus DDS-1, Bifidobacterium animalis subsp. Bacillus UABb-10 probiotics were clinically studied and found that these four probiotics had no significant effect on the treatment of constipation compared with the placebo group
Ibarra et al. studied the effect of bifidobacterium lactis subspecies HN019 on functional constipation, and found that it had no significant effect on the treatment of constipation
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Embodiment 1
[0038] Embodiment 1: the preparation of probiotic composition product
[0039] The preparation method of probiotic composition product:
[0040] Probiotic composition 1 was prepared by using bacterial powder, Bifidobacterium adolescentis CCFM626: Bifidobacterium breve CCFM622: Bifidobacterium animalis BB-115=2:1:1.
[0042] Mix Lactobacillus reuteri LR-168, Lactobacillus acidophilus LA-99 and Bifidobacterium animalis BB-115 uniformly according to the contents shown in Table 1 to prepare probiotic composition 3, wherein Reuteri Lactobacillus LR-168: Lactobacillus acidophilus LA-99: Bifidobacterium animalis BB-115=2:1:1.
[0043] Table 1
[0044]
[0045]
Embodiment 2
[0046] Embodiment 2: the in vivo effect of probiotic composition product
[0047] Experimental subjects: 6-week-old male SPF grade BABL / c mice.
[0048] Execution standard: The animal experiment protocol was approved by the Ethics Committee of Jiangnan University (JN.No20190930b0501220, JN.No20191115b0500110), and implemented in accordance with the EU guidelines (2010 / 63 / EU).
[0049] Experimental design: 70 mice were randomly assigned to blank group, model group, positive control group, 1-fold group-probiotic composition 3, 10-fold-group probiotic composition 3, probiotic composition 1 group, probiotic combination 2 groups of substances, 10 mice in each group, the ingredients and dosage of the test substances given to the mice in each group are shown in Table 2 below.
[0050] The test substance in the bacterium filling group was the probiotic composition product prepared in Example 1.
[0051] Table 2: Components and dosage of test substances given to mice in each group
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Abstract
The invention provides a probiotic composition and a preparation method and application thereof. The probiotic composition comprises raw materials of lactobacillus reuteri, lactobacillus acidophilus and bifidobacterium animalis, wherein the weight ratio of the lactobacillus reuteri to the lactobacillus acidophilus to the bifidobacterium animalis is (1.5-2.5):(0.5-1.5):(0.5-1.5). The colony forming units of the lactobacillus reuteri, the lactobacillus acidophilus or the bifidobacterium animalis in unit weight are the same. The lactobacillus reuteri is preserved in China Center for Type Culture Collection with the preservation number of 209138, and the lactobacillus acidophilus and/or the bifidobacterium animalis preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC 15210 are/is bifidobacterium animalis BB-115. The probiotic composition can relieve the symptom of slow transit constipation under the condition of low dosage, and can remarkably and effectively regulate gastrointestinal regulatory peptide indexes related to constipation, such as P substance (SP), gastrin (Gas) and vasoactive peptide (VIP).
Description
technical field [0001] The invention relates to the field of probiotics, in particular to a probiotic composition for treating intestinal diseases. Background technique [0002] Constipation is a phenomenon in which stools are hard or dry and difficult to pass, with fewer frequent bowel movements. Constipation is mostly caused by lack of dietary fiber. The onset of some constipation is related to intestinal microorganisms, such as bifidobacteria, which play an important role in maintaining normal intestinal peristalsis. Long-term constipation will cause a large amount of harmful substances in the feces to repeatedly stimulate the intestinal wall, which may cause rectal cancer. Therefore, long-term habitual constipation is not a normal performance and should be given attention. Some people use laxatives to fight constipation but make their condition worse, and the constipation becomes more and more frequent. [0003] Probiotics are live bacteria. They are bacteria that c...
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