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Method for recovering cadmium polluted soil by combining compound microorganism bacterium agent with houttuynia cordata

A technology of microbial complex bacteria and cadmium-contaminated soil, which is applied in the restoration of contaminated soil, etc., to achieve the effect of not causing secondary pollution, improving absorption capacity, and enhancing bioremediation

Inactive Publication Date: 2013-05-01
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in terms of the types and quantities of plants and soil microorganisms, it is difficult to construct an effective combination of plants and microorganisms to remediate heavy metal-contaminated soils, which also makes the practical application of this technology rarely

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The microbial composite bacterial agent of the present invention and Houttuynia cordata were used in pot experiments on cadmium-contaminated soil.

[0035] 1. Specific implementation method:

[0036] 1) Background detection: Take soil samples before inoculation to detect cadmium content, cadmium content in roots and stems of Houttuynia cordata, plant growth and various physical and chemical indicators of the soil.

[0037] 2) Prepare microbial compound bacteria agent. The microbial compound bacterial agent includes the following components in quantity, Pseudomonas fluorescens 7.5×10 9 CFU / m 3 , rumen anaerobic bacteria 3.8×10 8 CFU / m 3 , plexiphytic mycorrhizal fungi 6.3×10 8 CFU / m 3 . Preserve the compound microbial agent as a bacterial strain, take out a little bit to activate and expand the culture when using it, and use it to directly soak the roots of the plants after the expanded culture. The steps for the activation and expansion of the compound bacterial...

Embodiment 2

[0053] In this example, other ratios of bacteria in the microbial composite bacterial agent were used for pot experiments on cadmium-contaminated soil.

[0054] 1. Specific implementation methods

[0055] 1) Background detection. Soil samples were taken before inoculation with the compound bacterial agent to detect cadmium content, cadmium content in roots and stems of Houttuynia cordata, plant biomass and various physical and chemical indicators of the soil.

[0056] 2) Preparation of microbial compound bacterial agent. The microbial composite bacterial agent includes the following components in quantity: Pseudomonas fluorescens 1.2×10 9 CFU / m 3 , rumen anaerobic bacteria 4.5×10 8 CFU / m 3 , plexiphytic mycorrhizal fungi 3.4×10 7 CFU / m 3 .

[0057] 3) Inoculation of microbial compound bacteria agent. Irrigate the microbial compound bacterial agent on the soil, and the ratio of the microbial compound bacterial agent to the soil is 0.6L / m 2 .

[0058] 4) Clean and dis...

Embodiment 3

[0067] In this example, other proportions of the microbial compound bacterial agent were used in pot experiments on cadmium-contaminated soil.

[0068] 1. Specific implementation methods

[0069] 1) Background detection. Soil samples were taken before inoculation with the compound bacterial agent to detect cadmium content, cadmium content in roots and stems of Houttuynia cordata, plant biomass and various physical and chemical indicators of the soil.

[0070] 2) Preparation of microbial compound bacterial agent. The microbial composite bacterial agent includes the following components in quantity: Pseudomonas fluorescens 3.3×10 9 CFU / m 3 , rumen anaerobic bacteria 1.2×10 8 CFU / m 3 , bushy mycorrhizal fungi 1.3×10 7 CFU / m 3 .

[0071] 3) Inoculation of microbial compound bacteria agent. Irrigate the microbial compound bacterial agent on the soil, and the ratio of the microbial compound bacterial agent to the soil is 0.9L / m 2 .

[0072] 4) Clean and disinfect Houttuyn...

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Abstract

The invention discloses a method for recovering cadmium polluted soil by combining a compound microorganism bacterium agent with houttuynia cordata. The compound microorganism bacterium agent comprises the following components by count: 1*10<9>-9*10<9>CFU (colony forming unit) / m<3> pseudomonas fluorescens, 1*10<8>-6*10<8>CFU / m<3> actinomyces ruminicola, and 1*10<9>-9*10<9>CFU / m<3> glomus mosseae. The method for recovering the soil comprises the following steps that the houttuynia cordata is planted in cadmium soil and the compound microorganism bacterium agent is inoculated into the soil, or the houttuynia cordata is soaked in the compound microorganism bacterium agent and then planted into the cadmium soil; when branches and leaves of the houttuynia cordata are luxuriant, the branches and the leaves of the houttuynia cordata are harvested; and cadmium in the soil can be removed. The compound microorganism bacterium agent can better improve the tolerance of the houttuynia cordata, promote growth of a plant in the high-cadmium-content soil, improve the absorbility of the plant to cadmium, and enhance a soil heavy metal removal effect. The method for recovering the soil is a safe, environment-friendly, high-efficiency, economical and practical plant-microorganism combined method for recovering the heavy metal polluted soil, and has a significant application value.

Description

technical field [0001] The present invention relates to a technology of plant-microbial combined bioremediation of heavy metal polluted soil, more specifically, to a microbial composite bacterial agent used in cadmium-contaminated soil, and the joint restoration of cadmium-contaminated soil by microbial composite bacterial agent and plants Method, the microbial compound bacterial agent can strengthen the absorption of Houttuynia cordata on heavy metal-contaminated soil, so as to achieve the purpose of bioremediation of heavy metal-contaminated soil. Background technique [0002] With the rapid development of the modern economy, human activities have caused heavy metals such as cadmium, lead, zinc, and chromium to be continuously released into the environment, accumulate in the soil, and cause harm to the human body through the food chain, leaving the soil that people rely on for survival. brought serious pollution. As a highly toxic heavy metal element, the annual release o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
Inventor 陈文清周丽蓉
Owner SICHUAN UNIV
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