Biological restoration method for p-chloronitrobenzene compound polluted environment

A technology of chloronitrobenzene and p-chloronitrobenzene, applied in the restoration of contaminated soil, chemical instruments and methods, biological water/sewage treatment, etc., to restore ecological functions, improve physical properties, and promote utilization and degradation Effect

Active Publication Date: 2010-05-12
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few research reports on the degradation of chloronitrobenzene compounds at home and abroad, only a few reports on the microbial degradation of chloronitrobenzene, and the application of these microorganisms in actual bioremediation is rare

Method used

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  • Biological restoration method for p-chloronitrobenzene compound polluted environment
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  • Biological restoration method for p-chloronitrobenzene compound polluted environment

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Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1, detect the repair ability of the inventive method to the soilless substrate polluted by p-chloronitrobenzene compounds

[0029] Taking p-chloronitrobenzene as an example, the method for detecting the repair ability of the soilless substrate polluted by p-chloronitrobenzene compounds, the specific process comprises the following steps:

[0030] 1) Inoculate Comamonas testosteroni (Comamonas testosteroni) CNB-1 CGMCC No.1028 in an inorganic salt medium with p-chloronitrobenzene as a substrate, and vibrate at 28°C and 130rpm for 16 hours to obtain seeds liquid;

[0031] 2) Inoculate the seed solution of Comamonas testosteroni CNB-1 CGMCC No.1028 obtained in step 1) into LB liquid medium at an inoculation ratio of 1%, and culture it with shaking at 30° C. for 18 hours;

[0032] 3) Collect the bacteria by centrifugation at 6000rpm, and resuspend it in a sterile solution with a mass percent concentration of 0.9% NaCl to make the OD 600 Reach 2.0, obtain the ba...

Embodiment 2

[0037] Embodiment 2, detection method of the present invention is to the remediation ability of the soil polluted by p-chloronitrobenzene compounds

[0038] Taking p-chloronitrobenzene as an example, the pot experiment is used to detect the repair ability of the inventive method to the soil polluted by p-chloronitrobenzene compounds, and the specific process comprises the following steps:

[0039] 1) Inoculate Comamonas testosteroni (Comamonas testosteroni) CNB-1 CGMCC No.1028 in an inorganic salt medium with p-chloronitrobenzene as a substrate, and vibrate at 30°C and 130rpm for 16 hours to obtain seeds liquid;

[0040] 2) Inoculate the seed solution of Comamonas testosteroni CNB-1 CGMCC No.1028 obtained in step 1) into LB liquid medium at an inoculation ratio of 1%, and culture it with shaking at 28° C. for 18 hours;

[0041] 3) Collect the bacteria by centrifugation at 7000rpm, and resuspend it in a sterile 0.9% NaCl solution by mass percentage to make the OD 600 Reach 2....

Embodiment 3

[0048] Embodiment 3, Comamonas testosteroni (Comamonas testosteroni) CNB-1 CGMCC No.1028 in the detection of alfalfa root colonization ability

[0049] In order to facilitate the observation of the colonization of p-chloronitrobenzene-degrading strain Comamonas testosteroni (Comamonas testosteroni) CNB-1 CGMCC No.1028 in the plant root system during the bioremediation process, green fluorescent protein (green fluorescence protein, GFP ) gene is integrated on the genomic DNA of Comamonas testosteroni (Comamonas testosteroni) CNB-1, and the specific method is: (1) preparation of Comamonas testosteroni (Comamonas testosteroni) CNB-1 competent cell: summary of the invention The seed solution obtained in step 1) was inserted into LB liquid medium according to the inoculation amount of 1%, and cultured with shaking at 130rpm at 28°C to make its OD 600 reach 0.3-0.4, (the following operations are all carried out at 4°C) collect the bacterial cells by centrifugation at 4500rpm, resusp...

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Abstract

The invention discloses a biological restoring method of p-chloronitrobenzene polluting environment, which comprises the following steps: immersing the alfalfa seed with root in the OD600 1.8-2.2 (Comamonas testosteroni) CNB-1 CGMCC No.1028 bacteria suspension liquid to precede uterus; cultivating the alfalfa seed in the p-chloronitrobenzene polluting environment to restore the environment. The invention displays significant action of p-chloronitrobenzene polluted environment, which possesses key real appliance meaning.

Description

technical field [0001] The invention relates to a bioremediation method for a polluted environment, in particular to a bioremediation method for an environment polluted by p-chloronitrobenzene compounds. Background technique [0002] With the rapid development of my country's industry and agriculture, the problem of environmental pollution has increasingly become a very serious and urgent problem in the process of social development in our country. It not only restricts the economic development of our country and the improvement of people's living standards, but also seriously limits the The sustainable development of contaminated soil poses a great threat to people's health. Therefore, the restoration and treatment of polluted soil has become a problem of great concern. In the mid-1980s, the use of bioremediation technology to remediate polluted soil began to be applied. Years of research have shown that bioremediation is a technology that uses microorganisms or other organi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A62D3/00B09C1/10B09C1/00C02F3/34C02F1/58C12N1/20
Inventor 刘双江刘磊刘志培姜成英吴建峰
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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