50 results about "Comamonas testosteroni" patented technology

A fusion protein of pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) and a cytochrome is disclosed. PQQGDH is, for example, a water-soluble PQQGDH derived from Acinetobacter calcoaceticus. The cytochrome is, for example, an electron transfer domain of quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni. The fusion protein of the present invention shows intramolecular electron transfer from PQQ, a redox center, to the cytochrome, which allow construction of a direct electron transfer-type glucose sensor which requires no electron mediators.

The invention discloses a comamonas testosteroni strain for biological denitrificaion and application thereof. The comamonas testosteroni strain GAD4 is preserved in China General Microbiological Culture Collection Center (CGMCC for short) with preservation number CGMCC No. 2963 in March 19th, 2009. The strain is added to nitrogenous effluent with 2 to 6mg/L of dissolved oxygen to realize biological denitrificaion. The strain not only has the capability of heterotrophic nitrification, but also has the capability of aerobic denitrification. In the process of treating the nitrogenous effluent, only one aerobic stage is needed, the denitrification efficiency is high, and the operation is convenient and quick; and compared with the conventional biological denitrification process, the application has huge economic benefits.

The invention discloses a primotest clustered hair single cell bacterium (Comamonas testosteroni) WXZ-18 CGMCC No.1800 strain with aerobic denitrifying property and a method to denitrify the waste water; which is characterized by the following: possessing strong tolerance degree for dissolved oxygen; deacidizing nitrate and nitrite with distinctive speed under the condition of 10-98% saturated dissolved oxygen; seeding to active sludge; proceeding enriched culture; realizing the aerobic anti-nitration.

The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitriles to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.

The invention discloses a biological modification method of dacron fabrics, mainly comprising the following steps: 1. culturing strains: culturing Comamonas testosteroni in a agarslant solid culture medium for 12-18h under the temperature of 30-37 DEG C, and storing in a refrigerator at the temperature of 4 DEG C; 2. preparing a dacron modified liquid: preparing 6 parts of dacron modified liquid with the terephthalic acid concentration of 1-3g/L (each part is 150ml), respectively filling in a conical flask, and packing and sterilizing for 25min under the temperature of 121 DEG C; 3. modifying the dacron fabrics: performing ultraviolet sterilization for 0.5h on 6 parts of dacron fabrics, implanting the Comamonas testosteroni in 3 parts of dacron modified solutions, adding 3 parts of dacron fabrics into the 3 parts of dacron modified liquid respectively, taking the other three parts of dacron fabrics as a reference, performing shake cultivation at the temperature of 30-37 DEG C and with the revolving speed of 200r/min, and performing catalytic modification on the dacron fabrics; and 4) flushing the fabrics by tap water trickles and distilled water in turn, cleaning the fabrics by ultrasonic and airing.

The invention relates to a Comamonas testosteroni engineering bacterium. Directional regulation is adjusted and controlled to Comamonas testosterone through a DNA recombination technology, so as to produce Comamonas testosteroni AMCa 3 (CGMCC No.2364) having the capability of efficiently degrading steroid compounds. The engineering bacterium can effectively degrade testosterone, cholesterol and other steroid compounds, has good degradation effect to soils and livestock-poultry excreta seriously polluted by steroid compounds, and can be used for the treatment of livestock-poultry excreta, producing low-cholesterol food in food industry, and transforming steroid substances in the production of feed additives and dairy products for producing pollution-free food. The engineering bacterium has important significance in environmental remediation and agricultural application.

The invention relates to application of a microbial agent to malodorous gas pollution regulation. Malodorous gas is nitrogen-containing or sulfur-containing inorganic/organic malodorous gas or volatile heterocyclic organic gas, and the microbial agent is formed by comamonas testosteroni LH-N5, achromobacter xylosoxidans subsp. xylosoxidans LH-N25CGMCC No.6972 and Paracoccus aminovorans LH-N40CGMCC No.6971 in any proportion. The microbial agent has a remarkable deodorization effect, can tolerate or degrade organic and toxic pollutants, is particularly suitable for deodorization and purification of nitrogen-containing or sulfur-containing inorganic/organic waste gas, is suitable for regulation of volatile heterocyclic toxic organic malodorous gas, has a simple treatment process and stable effects, is resistant to environmental toxicant impact, and has wide application prospect in malodorous gas pollution control.

The invention discloses a biological restoring method of p-chloronitrobenzene polluting environment, which comprises the following steps: immersing the alfalfa seed with root in the OD600 1.8-2.2 (Comamonas testosteroni) CNB-1 CGMCC No.1028 bacteria suspension liquid to precede uterus; cultivating the alfalfa seed in the p-chloronitrobenzene polluting environment to restore the environment. The invention displays significant action of p-chloronitrobenzene polluted environment, which possesses key real appliance meaning.

The invention discloses microbial flora capable of rapidly degrading estrogen and analogs in chicken manure and belongs to the technical field of microbial engineering. The microbial flora comprises equal amounts of Comamonas testosteroni ATCC11996, KF1-Fe (Comamonas testosteroni ATCC49202), Ca3, Cb7, M6, M2, A3, vibrio H5 and Brucella S19-1. The environment adaptability of strains and the degradation efficiency of the estrogen and analogs are improved under the coordinate symbiosis action of compound microbial flora, and the risk caused by environmental pollution to ecology and human health is effectively reduced.

The invention belongs to the technical field of biological engineering, and in particular relates to comamonas testosteroni HHALA-001 and a method for using the comamonas testosteroni HHALA-001 to produce L-alanine; the preservation number of the strain is CGMCCNo.14064; the comamonas testosteroni HHALA-001 is obtained by compound mutation and repeated screening, the strain has high enzyme activity, the enzyme activity can reach 11.80U-12.22U, the strain can be used for high expression of L-aspartate beta-decarboxylase, is used for production of the L-alanine by catalysis of L-aspartic acid, can greatly improve production efficiency and conversion rate of the L-alanine, helps to reduce production cost, and has good stability; after use of the strain, the conversion rate of the L-Aspartic acid can reach 99.9%; the comamonas testosteroni HHALA-001 fully meets industrial L-alanine strain mutagenesis and screening demands, can be used in industrial production, and has great economic value.

The invention discloses a compound microbial agent. The compound microbial agent comprises the following raw material components: a pseudomonas japonica culture, a comamonas testosteroni culture, a microbacillus culture and an acidovorax delafieldii culture; according to the invention, multiple bacterial cultures are subjected to mixed fermentation to prepare the compound microbial agent; and thecompound microbial agent has an effective degradation effect on organic residues such as nitrobenzene compounds formed by insecticides or pesticides in soil, and furthermore, special strains are subjected to mixed cultivation and multiple strains are subjected to mixed fermentation to provide a simple and easy preparation method.

The invention relates to a construction of a Comamonas testosteroni 3,17beta-HSD gene reinforced strain, and belongs to the microbial field. The construction of the Comamonas testosteroni 3,17beta-HSD gene reinforced strain is characterized in that a recombinant plasmid is pK-Tacpromoter-3,17beta-HSD; and the construction comprises the following steps: carrying out PCR technological amplification, and target segment recovery and purification of a Comamonas testosteroni genome which is a template to connect with a pUCm-T carrier; 2, carrying out PCR technological amplification, and target segment recovery and purification of a plasmid pAH10 which is a template to connect with the pUCm-T carrier; 3, extracting a recombinant plasmid 3,17beta-HSD-T through adopting a column plasmid DNA small-amount extraction kit; and 4, respectively extracting the recombinant plasmid and pK-3,17beta-HSD through adopting the column plasmid DNA small-amount extraction kit. According to the invention, the Comamonas testosteroni 3,17beta-HSD gene reinforced strain is constructed for the first time and is applied, and the estriol degradation efficiency, the cholesterol degradation efficiency and the stosterone degradation efficiency of the Comamonas testosteroni 3,17beta-HSD gene reinforced strain are 30%, 15% and 150% higher than that of a wild Comamonas testosteroni strain respectively.

The invention relates to a method for treating bird waste water by a novel mixed bacterium microbial fuel cell. The method includes the steps: activating and then inoculating comamonas testosteroni and desulfovibrio into culture solution; culturing the closed comamonas testosteroni in a shaker, culturing the closed desulfovibrio in an incubator at constant temperature until a last phase of a logarithmic phase; uniformly mixing the cultured comamonas testosteroni and the cultured desulfovibrio, mixing mixture, nutrient solution, buffer solution and sludge according to the volume ratio of (1-3):(2-5):(1-3):(3-7), inoculating mixture into an anode chamber of the microbial fuel cell, enabling floras to be suspended in anode solution or adhered on an anode, and standing to degrade the bird waste water and generate power; updating anode solution once every 2-7 days, and retaining 10% of original mixture in the original anode chamber. The method has the advantages that mixed bacteria of the comamonas testosteroni and the desulfovibrio are applied to microbial fuel cells based on treatment of bird waste water, the degradation capability of the bird waste water can be effectively improved,and electric energy is generated.