Para-chloro nitrobenzene degrading testosterone coma monad and its use

Abstract

The present invention relates to one Comamonas testosteroni CNB1CGMCC No. 1028 strain, whose growing cell, cell suspension and immobile cell can degrade para-chloronitrobenzene. The strain can grow with para-chloronitrobenzene as unique carbon source, nitrogen source and energy source, so as to degrade and utilize para-chloronitrobenzene completely. During its growth, the strain first reduces nitro group into hydroxylamine, and produces 2-aminophenol 1, 6-bioxygenase to open the ring of 2-amino-5-chlorophenol into hydrocarbon through meta ring opening. The strain is suitable for the biological treatment of industrial effluent from para-chloronitrobenzene production and the biological repair of para-chloronitrobenzene polluted soil.

Description

technical field

[0001] The invention belongs to the field of microbial biotechnology and environmental biotechnology, in particular, it relates to a Comamonas testosteroni CNB1 CGMCC No.1028 bacterial strain, growth cells, cell suspensions, immobilized cells of the bacterial strain The bacteria can degrade p-chloronitrobenzene; the strain can grow with p-chloronitrobenzene as the only carbon source, nitrogen source and energy source, and completely degrade and utilize p-chloronitrobenzene. When the strain grows with p-chloronitrobenzene as the only carbon source, nitrogen source and energy source, it first reduces the nitro group to hydroxylamine, and then under the action of 2-aminophenol 1,6-dioxygenase produced by the strain , through meta ring opening, 2-amino-5-chlorophenol is ring-opened into hydrocarbons, which can be further degraded and utilized. The bacterial strain is suitable for biological treatment of industrial wastewater producing p-chloronitrobenzene and bior...

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