Delftia with aerobic denitrifying capability and method of treating waste water by the same
A technology for aerobic denitrification and wastewater treatment, applied in aerobic process treatment, chemical instruments and methods, sustainable biological treatment, etc., can solve problems such as anoxic denitrification
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Embodiment 1
[0015] Embodiment 1: Determination method of aerobic denitrification performance of Delftia tsuruhatensis CGMCC No.1797 strain
[0016] In order to prevent the introduction of miscellaneous bacteria into the simulated wastewater, both the simulated nitrate wastewater I and the simulated nitrate wastewater II were inactivated in a high-temperature sterilization pot at 0.1 MPa for 20 minutes. Get 5ml of sterilized nitrate simulated waste water I and put it into a test tube, insert the pure Delftia tsuruhatensis CGMCC No.1797 bacterial strain sample preserved with liquid paraffin seal. The test tube was placed in an air bath constant temperature shaker, the constant temperature was maintained at 30°C, and the shaker speed was 160r / min to activate the strain. After activating for 12 hours, inoculate 10% of the inoculum into a 250ml Erlenmeyer flask filled with 200ml nitrate simulated waste water II to measure denitrification and denitrification performance. In order to ensure the...
Embodiment 2-10
[0020]Example 2-10: Different experimental conditions and ranges determine the optimal denitrification conditions for Delftia tsuruhatensis CGMCCNo.1797 strain
[0021] The present invention carries out 9 wastewater treatment tests in total, preferably the aerobic denitrification reaction conditions of Delftia tsuruhatensis WXZ-9CGMCC No.1797 strain.
[0022] The four reaction factors of wastewater aerobic denitrification are: dissolved oxygen concentration, which is realized by controlling different rotational speeds rpm. The three levels of rotational speed are 60, 100 and 160 rpm, and the corresponding dissolved oxygen concentrations are 1.8-3.5 mg / L ( 24~46% saturated dissolved oxygen), 4.2~5.6mg / L (55~74% saturated dissolved oxygen) and 6~7.2mg / L (79~95% saturated dissolved oxygen); the three levels of nitro nitrogen concentration are respectively The three levels of carbon-nitrogen ratio are 2, 6 and 10 respectively; the three levels of pH are 6.5, 7.0 and 7.5 respective...
Embodiment 11
[0027] Embodiment 11: PCR amplification and sequence determination of the 16SrRNA gene of Delftia tsuruhatensis CGMCC No.1797
[0028] Delftia tsuruhatensis CGMCC No.1797 was inoculated into 100 μL of double distilled water, boiled in a boiling water bath for 5 minutes, and colony PCR amplification was performed. The primers used for the 16S rRNAPCR reaction were a pair of universal primers, that is, the forward primer was f27 (5'-AGAGTTGATCCTGGCTCAG-3') and the reverse primer was r1492 (5'-GGTTACCTTGTTACGACTT-3'). Primers were synthesized by Beijing Huada Zhongsheng Technology Development Co., Ltd. PCR reaction system (50 μL): 5 μL of 10×Buffer, 4 μL of dNTPs, 1 μL of primers f27 and r1492, 39 μL of double distilled water, centrifuged and mixed, and then added 0.5 μL of DNA template and 0.5 μL of Taq enzyme. The PCR reaction program included: (1) pre-denaturation at 94°C for 5 min; (2) denaturation at 94°C for 1 min; (3) annealing at 50°C for 1 min; (4) extension at 72°C for...
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