Method for producing L-alanine by bioconverting L-aspartic acid by free cell process
A technology of free cells and aspartic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as side reactions, large equipment investment, and complicated separation
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Embodiment 1
[0051] culture medium
[0052] Activated slant medium (g / L): sodium glutamate 10.0, peptone 8.0, sodium chloride 5.0, magnesium sulfate 1.0, potassium dihydrogen phosphate 0.5, yeast extract 5.0, beef extract 4.0, agar 20.0, adjust pH 7.0- 7.2.
[0053] Shake flask seed medium (g / L): sodium glutamate 15.0, peptone 10.0, corn steep liquor dry powder 5.0, sodium hydroxide 6.0, magnesium sulfate heptahydrate 0.25, dipotassium hydrogen phosphate 0.12, adjust pH to 7.0.
[0054] Fermentation medium (g / L): peptone 12.0, corn steep liquor powder 4.0, sodium glutamate 12.0, sodium hydroxide 6.0, magnesium sulfate heptahydrate 0.30, dipotassium hydrogen phosphate 0.12, adjust the pH to 7.0 with sodium hydroxide solution.
[0055] Take a ring of Comamonas testosteroni HY-08D slant strain and connect it to the slant medium, culture at 35°C±1°C for 18h, then take a ring of strain and connect it to 150mL shake flask medium (inoculate several bottles), 150rpm , and cultivated at 28°C for...
Embodiment 2
[0057] culture medium
[0058] Activated slant medium (g / L): sodium glutamate 12.0, peptone 10.0, sodium chloride 7.0, magnesium sulfate 1.2, potassium dihydrogen phosphate 0.7, yeast extract 7.0, beef extract 3.0, agar 20.0, adjust pH 7.0- 7.2.
[0059] Shake flask seed medium (g / L): sodium glutamate 17.0, peptone 12.0, corn steep liquor dry powder 7.0, sodium hydroxide 8.0, magnesium sulfate heptahydrate 0.35, dipotassium hydrogen phosphate 0.08, adjust pH to 7.0.
[0060] Fermentation medium (g / L): peptone 15.0, corn steep liquor powder 5.0, sodium glutamate 15.0, sodium hydroxide 6.0, magnesium sulfate heptahydrate 0.10, dipotassium hydrogen phosphate 0.15, adjust the pH to 7.0 with sodium hydroxide solution.
[0061] Take a ring of Comamonas testosteroni HY-08D slant strain and connect it to the slant medium, culture at 25°C±1°C for 40h, then take a ring of strain and connect it to 150mL shake flask medium (inoculate several bottles), 100rpm , and cultured at 32°C for ...
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