203 results about "L-alanosine" patented technology

3,4-Dihydroxyphenyl-L-alanine (DOPA) is an unusual amino acid found in mussel adhesive proteins (MAPs) that form tenacious bonds to various substrates under water. DOPA is believed to be responsible for the adhesive characteristics of MAPs. This invention relates to a route for the conjugation of DOPA moieties to various polymeric systems, including but not limited to poly(ethylene glycol) or poly(alkylene oxide) systems such as poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) block copolymers.

A composition providing an all-natural, non-surgical preventative of or improvement to disorders of the prostate gland is described. The invention relates to a composition for the prevention of or improvement of prostatitis, and for relieving symptoms and improving objective signs of prostatitis. The formula of the composition preferably includes the following ingredients each in a therapeutically effective amount: Vitamin C, Vitamin B6, Vitamin E, zinc, glycine, L-alanine, Glutamic acid, Saw palmetto, Pygeum extract, Pumpkin seed, Stinging nettle, Echinacea, garlic, Ginkgo leaves, and selenium.

Methods for treating and preventing the onset and maintainance of multiple drug resistance (MDR) in animals undergoing chemotherapy for cancer are provided. According to the methods, target cells are depleted of adenosine 5'-monophosphate (AMP) and adenosine 5'-triphosphate (ATP) such that the cells are unable to support P-glycoprotein activity. According to one method, a population of target cells is obtained from a host and assayed for loss of methylthioadenosine phosphorylase (MTAse) activity. MTAse catabolizes methylthioadenosine to adenine for endogenous salvage incorporation into the intracellular AMP pool. MTAse deficient cells are treated with a purine synthesis inhibitor, such as L-alanosine, which starves the cells of adenine and suppresses P-glycoprotein activity. MTAse competent cells are also treated for MDR with purine synthesis inhibitors. In conjunction with treatment according to the invention, MTAse competent and deficient cells are also treated for malignancy with other anti-cancer drugs. A method for protecting non-malignant cells from adenine starvation during treatment of malignant cells according to the invention is provided.

The invention discloses a preparation method of a ribofuranose phosphate derivative. The preparation method comprises preparation steps as follows: L-alanine isopropyl ester hydrochloride, phenol dichlorophosphate and substituted phenol are taken as starting materials and have a docking reaction under the action of alkali; (2R)-2-deoxy-2-difluoro-2-methyl-D-erythropentonic acid GAMMA-lactone and 3,5-dibenzoate reduce carbonyl in a dichloromethane or ether solvent into an alcoholic hydroxyl group under the action of a strong reducing agent; an intermediate with a formula 2-1 has a reaction with paratoluensulfonyl chloride under the action of alkali to obtain p-toluenesulfonates; an intermediate with a formula 2-2 and a benzoyl cytosine derivative have a docking reaction under the action of a condensing agent; an intermediate with a formula 2-3 converts cytosine into uracil under the action of organic acid; benzoyl protection for an intermediate with a formula 2-4 is released under the action of an alkaline agent; an intermediate with a formula 2-5 and an intermediate with a formula 1 are docked under the action of a Grignard reagent to obtain Sofosbuvir.

The invention relates to an amino acid composite reinforcement method for rotten silk cultural relics, which is characterized by comprising the following steps: A) using a spraying device to uniformly spray amino acid solution with the mass percentage concentration being 0.5-2 percent onto the surfaces of the rotten silk cultural relics until water can drop down; wherein the amino acid is one of L-cysteine, L-alanine, L-lysine, L-histidine and L-arginine; and B) waiting for 10min after completion of Step A, uniformly spraying ethylene glycol diglycidyl ether solution with the mass percentage concentration being 1.5-3 percent onto the surfaces of the rotten silk cultural relics until water can drop down, and naturally drying the rotten silk cultural relics, thereby reinforcing the rotten silk cultural relics. Compared with the prior art, the method has the advantages that: 1) the operation is more convenient; and 2) the reinforcing effect is obviously better than the effect of the original method, the operation is more convenient and complies with the requirements and concepts of bionic reinforcement.

The invention discloses escherichia coli for efficiently producing L-alanine by fermentation, and belongs to the technical field of microbial metabolic engineering. Coding genes for a synthesis route for byproducts acetic acid, formic acid, alcohol, succinic acid and lactic acid on a chromosome of escherichia coli CGMCC No.10628 provided by the invention are deleted, and the chromosome dadX gene is replaced as an alanine dehydrogenase gene, wherein the coding genes comprise ackA-pta, pflB, adhE, frdA and idhA. The recombinant bacterium is fermented for 26 hours to produce 106g/L or more than 106g/L L-alanine with high optical purity and high chemical purity at 28-45 DEG C; and in the whole fermentation process, the production strength reaches 4.27g/L.h or more.

The invention discloses a composition for relieving physical fatigue and enhancing an immunologic function, a preparation method thereof and application thereof. The composite comprises 10 to 100 weight parts of guarana extract, 100 to 800 weight parts of taurine, 1 to 10 weight parts of L-lysine hydrochloride, 1 to 10 weight parts of L-isoleucine, 0.1 to 5 weight parts of L-leucine, 0.2 to 5 weight parts of L-valine, 1 to 15 weight parts of L-aspartate, 0.01 to 0.3 weight part of L-tryptophan, 0.01 to 0.3 weight part of L-phenylalanine, 0.01 to 0.3 weight part of L-threonine, 0.01 to 0.3 weight part of L-methionine, 10 to 100 weight parts of L-alanine, 10 to 100 weight parts of glycine, 1 to 15 weight parts of tea polyphenol and 1 to 20 weight parts of American ginseng extract.

The invention relates to a healthcare product for relieving hangover and protecting liver. The healthcare product comprises the following materials based on ratio: 1 to 10 parts of acetaldehyde dehydrogenase enzymic preparation, 20 to 50 parts of pueraria extract, 10 to 30 parts of ginseng extract, 5 to 15 parts of acanthopanax senticosus extract, 5 to 10 parts of langehead atractylodes rhizome extract, 10 to 15 parts of lycium barbarum extract, 20 to 40 parts of hovenia dulcis thumb, 0 to 2 parts of vitamin B1, 0 to 2 parts of vitamin B2, 0 to 1 part of vitamin B6, 1 to 5 parts of vitamin C, 0 to 1 part of L-glycine, 1 to 2 parts of L-cysteine, and 0 to 2 parts of L-alanine. The healthcare product comprises the following functions: 1, the hangover is relieved, and the recovery after drink is sped up; 2, various symptoms after drink are relieved, the inbibitional effect of alcohol to nervous centralis is reduced, and the conscious can be effectively maintained; 3, the effect of detoxifying the liver is improved, the lipid peroxidation reaction is inhibited, the damage of the alcohol to the liver is reduced, and the regeneration capacity of the stem cells is improved; and 4, the simulation of the alcohol to the stomach is relieved.

The invention discloses a strain of engineering bacteria producing DL-alanine. Lactic dehydrogenase, pyruvate formate lyase, alcohol dehydrogenase, acetic acid kinase, fumaric acid reductase, alanine racemase and methyl glyoxal synthetase of the strain of engineering bacteria producing the DL-alanine are inactivated; and exogenous L-alanine dehydrogenase gene and alanine racemase gene are integrated on the chromosome of the engineering bacteria. According to the invention, pyroracemic acid, an intermediate product of the glycolysis is converted to L-alanine by integrating the exogenous L-alanine dehydrogenase gene into the chromosome of the engineering bacteria; and an exogenous alanine racemase gene is further integrated into the chromosome, and part of the L-alanine is converted into D-alanine. Then producing the DL-alanine from raw material sugar in one step is realized, the production period of the DL-alanine is decreased and the productivity of the DL-alanine is enhanced.

The invention discloses barbary wolfberry fruit chicken essence, which comprises barbary wolfberry fruit, sodium glutamate, edible salt, edible corn starch, white granulated sugar, maltodextrin, chicken powder, chicken extract seasoning, L-lactamine, disodium succinate, aminoacetic acid, curry powder, spices, wheat extract powder, eggs, flavor-developing nucleotide disodium, edible essence and spices and curcuma in the weight ratio of 10:38:12:4.5:6:5.5:4:5:1.4:1.5:1.3:1.5:1.1:1.8:1.3:1.5:1.7:1.9. The invention further provides a method for producing the barbary wolfberry fruit chicken essence.

The present invention discloses L-alanine dehydrogenase mutant zymoprotein and a preparation method thereof. According to a homologous sequence secondary structure comparison result, site-specific mutagenesis of 73th lysine near an L-alanine dehydrogenase activity center of Bacilluspseudofirmus into alanine through PCR is performed to construct a mutant expression vector, and prokaryotic expression and Ni-NTA affinity chromatography purification are performed to obtain a mutant enzyme K73A, wherein specific activity of the obtained mutant enzyme K73A is 202% of specific activity the wild type, and other enzymatic properties are not changed. In addition, a turnover number Kcat on a substrate L-alanine by the mutant zymoprotein is 376.7 min<-1> and is 2.0 times the turnover number Kcat of the wild type, a turnover number Kcat on beta-NAD<+> by the mutant zymoprotein is 290.1 min<-1> and is 2.1 times the turnover number Kcat of the wild type, and the mutant zymoprotein can be applicable for production processes of production of pyruvic acid, L/D-alanine, and the like through improvement biology methods.

The invention relates to a bacillus amyloliquefaciens insecticide-fertilizer for farm onsite fermentation, a preparation method and applications thereof. The insecticide-fertilizer comprises the following raw materials: bacillus amyloliquefaciens, colistin sulfate, natamycin, L-alanine, asparagine, yeast extract powder, peptone, disodium hydrogen phosphate, sodium chloride, glucose, fructose, and brown sugar. The colistin sulfate and natamycin in the insecticide-fertilizer can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (alanine, fructose, and asparagine) can rapidly promote the germination of spores of bacillus amyloliquefaciens and improve the germination rate of spores. The provided insecticide-fertilizer can be applied to the onsite fermentation and propagation in a simple and crude farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content and good activity. The insecticide-fertilizer can prevent disease, promote growth, and increase output and improve quality, and can be used as a microbial pesticide, and the effect is stable.

The invention discloses a method of producing L-alanyl-L-glutamine from recombinant escherichia coli, wherein the method of producing L-alanyl-L-glutamine from recombinant escherichia coli is follows: in an aqueous solution with a determined pH value, acting on free L-glutamine and L-alanine methyl ester hydrochloride to generate L-alanyl-L-glutamine, recombining a gene segment of the protein with the amino-acid ester acyltransferase of the L-alanyl-L-glutamine into a carrier and transferring into host bacteria, thereby obtaining the host bacteria with the recombinant DNA and capable of strengthening the activity of an L-alanyl-L-glutamine biological synthesis system. The method comprises the following steps: (1) culturing a larger number of recombinant escherichia coli cells for expressing the amino-acid ester acyltransferase; (2) excessively expressing the amino-acid ester acyltransferase in the step (1); and (3) taking the amino-acid ester acyltransferase in the step (2) as a crude enzyme source, adding the crude enzyme source into a buffer solution containing L-glutamine and L-alanine methyl ester hydrochloride substrate amino acid to react, thereby realizing efficient production of the L-alanyl-L-glutamine.

The invention relates to a paenibacillus polymyxa pesticide-fertilizer for farm onsite fermentation, a preparation method, and applications thereof. The pesticide-fertilizer comprises the following raw materials: paenibacillus polymyxa powder, colistin sulfate, natamycin, L-alanine, valine, yeast powder, corn steep liquor powder, disodium hydrogen phosphate, sodium chloride, fructose, and brown sugar. The colistin sulfate and natamycin in the insecticide-fertilizer can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (alanine, fructose, and valine) can rapidly promote the germination of spores of paenibacillus polymyxa and improve the germination rate of spores. The provided pesticide-fertilizer can be applied to the onsite fermentation and propagation in a simple and crude farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content and good activity. The insecticide-fertilizer can prevent disease, promote growth, and increase output and improve product quality, and can be used as a microbial pesticide, and the effect is stable.

The invention discloses escherichia coli capable of producing L-alanine through fermentation and application of the escherichia coli and belongs to the technical field of biological engineering. According to the escherichia coli, aspartase AspA and L-aspartic acid-beta-decarboxylase AsD are introduced into the escherichia coli capable of producing fumaric acid through the fermentation; furthermore, a YgaW gene is over-expressed to strengthen the transport ability of the L-alanine in recombinant bacteria, so that a novel strain capable of fermenting glucose to produce the L-alanine is constructed; when the recombinant strain is fermented in a fermentation tank for 45h, the yield of the L-alanine reaches 147g/L and the saccharic acid conversion rate is 79.0 percent.