3 results about "Pyruvate oxidase" patented technology

This invention discloses a periodontitis-targeted antibacterial supramolecular material, its preparation method, and its application. It belongs to the field of catalyst technology. The preparation method of the periodontitis-targeted antibacterial supramolecular material includes the following steps: dissolving 2,5-dihydroxyterephthalic acid and tetrakis(4-aminophenyl)porphyrin in a first solvent, adding a first catalyst to react, and obtaining a DHA-TAPP COF skeleton; dispersing the DHA-TAPP COF skeleton in a second solvent, adding 3-bromopropyltrimethylammonium bromide and a second catalyst to react, and obtaining Q-COF; dispersing Q-COF in a third solvent, mixing it with H2PtCl6 solution, and then placing it under light conditions to obtain Pt-Q-COF; dispersing Pt-Q-COF in a buffer solution, adding pyruvate oxidase for adsorption treatment, and obtaining POx-Pt-Q-COF. This invention achieves a synergistic effect of "targeted enrichment - dual ROS generation - closed-loop H2O2 supply" through material structure design and functional unit integration, thereby improving the clearance efficiency of periodontal pathogens while ensuring biocompatibility.

The present application provides a recombinant E. coli for synthesizing heme and derivatives thereof, wherein the recombinant E. coli is knocked out of genes such as pyruvate oxidase poxB, pyruvate formate-lyase pflB and protoporphyrinogen peroxidase yfeX, and meanwhile, the genes such as phosphopyruvate carboxylase ppc, glycerol transporter glpF and 3-phosphoglycerate dehydrogenase glpD are enhanced, and the genes such as 5-aminovaleryl-CoA synthase hemA and ferrous chelatase hemH are introduced, so that the recombinant E. coli can synthesize heme and derivatives thereof by using glycerol as a raw material, and has application prospects in the field of biological manufacturing.

The application discloses a recombinant ammonium acetate-producing bacterium and a method for preparing ammonium acetate, and belongs to the technical field of bioengineering.The application aims to provide a method for producing ammonium acetate.The application provides a recombinant ammonium acetate-producing bacterium, which is obtained by knocking out the genes poxB of pyruvate oxidase, pflB of pyruvate formate-lyase and the key subunit aceE of pyruvate dehydrogenase of Escherichia coli as a starting bacterium, and overexpressing the genes Agx1 / BhcA of glyoxylate aminotransferase, Dhaa / BhcC of hydroxyaspartate aldolase, BhcB of beta-hydroxyaspartate dehydratase and BhcD of iminosuccinate reductase.The application combines the carbon sequestration path with the fermentation process optimization, realizes direct and efficient synthesis from glucose to ammonium acetate, significantly simplifies the process flow, and improves the carbon atom utilization efficiency and product yield.