51 results about "Alanine racemase" patented technology

The invention discloses an XZ-A26 bacterial strain for producing L-alanine with high yield, which has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.4036 and has the capacity of generating high-concentration L-alanine through fermentation. The XZ-A26 bacterial strain is constructed by the steps of: integrating an L-alanine dehydrogenase gene on thermophilic fatty bacillus chromosome on lactic dehydrogenase position on an escherichia coli ATCC8739 chromosome, then sequentially knocking out a pyruvate formate lyase gene, an alcohol dehydrogenase gene, an acetokinase gene, a fumaric acid reductase gene and an alanine racemase gene of the escherichia coli chromosome, and then carrying out continuous cell culture in a fermenting tank for obtaining agenetic engineering strain. The invention also relates to a construction method of the XZ-A26 bacterial strain and an application of the XZ-A26 bacterial strain in preparation of the L-alanine. According to the invention, the escherichia coli with the preservation number of CGMCC No.4036 for generating the high-concentration L-alanine through fermentation can be constructed by using a metabolic engineering method, and the yield of the L-alanine generated by using the XZ-A26 bacterial strain reaches up to 115g / L. The XZ-A26 bacterial strain is suitable for industrially producing the L-alanine.

Disclosed is an alanine racemase chiral binaphthol derivative having the ability to recognize amino alcohols selectively on the basis of chirality and transform amino acids from an L-form into a D-form. Methods for the optical resolution of amino acid or amino alcohol and for the optical transformation of D- and L-forms of amino acids using the binaphthol derivative are also provided.

The invention discloses an XZ-A26 bacterial strain for producing L-alanine with high yield, wherein the preservation number is CGMCC No.4036. The XZ-A26 bacterial strain has the ability of fermenting to produce high-concentration L-alanine and is genetic engineering bacteria prepared by integrating the L-alanine dehydrogenase gene on Escherichia Coli chromosome of a lipase gene from Geobacillus Stearothermophilus at the lactic dehydrogenase of Escherichia coli ATCC 8739 chromosome, then sequentially removing a pyruvate formate-lyase gene, an ethanol dehydrogenase gene, an acetokinase gene, a fumarate reductase gene and an alanine racemase gene of the escherichia coli chromosome and then continuously subculturing in a fermentation tank. The invention also relates to a construction method of the bacterial strain and the application of the bacterial strain in preparing the L-alanine. According to the metabolic engineering method, the escherichia coli CGMCC No.4036 capable of fermenting to produce the high-concentration L-alanineis constructed, and the yield of the L-alanine produced by fermenting the bacterial strain is as high as 115g / L. The invention is suitable for the industrial production of L-alanine.

The present disclosure provides recombinant Staphylococcus bacterium (e.g. S. epidermidis) that are dependent on D-alanine for growth. In one aspect, the disclosure features a recombinant Staphylococcus bacterium comprising two inactivated alanine racemase genes (Δalr1Δalr2); and an inactivated D-alanine aminotransferase (dat) gene. In another aspect, the disclosure features a method of making the recombinant Staphylococcus bacterium.