2 results about "Alanine racemase" patented technology

The application discloses a mutant D-amino acid dehydrogenase and a method for preparing D-phenylalanine through a multi-enzyme cascade, wherein the mutant D-amino acid dehydrogenase is obtained by site-directed mutagenesis of wild-type amino acid dehydrogenase of symbiotic bacillus licheniformis; the multi-enzyme cascade preparation method is to use cheap L-phenylalanine as raw material, first obtain DL-phenylalanine through catalysis of alanine racemase, and then convert L-phenylalanine into D-phenylalanine through L-amino acid deaminase, mutant D-amino acid dehydrogenase and formic acid dehydrogenase. The concentration of D-phenylalanine product obtained by the method reaches 80-120 g / L, and the conversion rate can reach 95-98 %, so that the method not only shortens the production cycle and has high product concentration, but also is easy to industrialize.

To provide recombinant Staphylococcus bacteria (e.g., S. epidermidis) that are dependent on D-alanine for growth. [Solution] In one aspect, the Disclosure features recombinant Staphylococcus bacteria comprising two inactivated alanine racemase genes (Δalr1Δalr2); and an inactivated D-alanine aminotransferase (dat) gene. In another aspect, the Disclosure features a method for producing recombinant Staphylococcus bacteria. In another aspect, the Disclosure provides a method for treating or preventing a rash in a subject, the method comprising the step of administering to the subject an amount effective to treat or prevent a rash in the subject, which is one of the aspects or embodiments described herein.