227results about How to "High chiral purity" patented technology

A method of selectively preparing a chiral 2S-amino alcohol useful in preparation of an amide sulfonated or acylated with alkyl, substituted aryl or substituted heteroaryl is described. The method involves reacting a di-tert-butyl diazene-1,2-dicarboxylate with a (4S)-4-benzyl-3-[(S)-trifluoromethyl-alkyl substituted alkanoyl]-1,3-oxazolidin-2-one to afford a di-tert-butyl 1-(1S,2S)-([(4S)-4-benzyl-2-oxo-1,3-oxazolidine-3-yl]-carbonyl}-trifluoromethyl-alkyl substituted alkyl)hydrazine-1,2-dicarboxylate. This dicarboxylate is then reduced to yield di-tert-butyl 1-(1S,2S)-[trifluoromethyl-alkyl substituted alkyl]hydrazine-1-(hydroxymethyl)-1,2-dicarboxylate. The resulting product is deblocked with an acid to yield the acid addition salt of (2S,3S)-trifluoro-hydrazino-methyl alkan-1-ol. The acid addition salt of (2S,3S)-trifluor-2-hydrazino-methyl alkan-1-ol is hydrogenated in the presence of a suitable metal catalyst to yield the amino alcohol (2S,3S)-2-amino-trifluoro-methyl alkan-1-ol HCl.

The invention discloses a glufosinate-ammonium dehydrogenase mutant and an application thereof to production of L-glufosinate-ammonium through oxidation-reduction of multienzymes. According to the method, a method for preparing an L-glufosinate-ammonium precursor through D-amino acid oxidase is utilized, D, L-glufosinate-ammonium or D-glufosinate-ammonium is used as substrate, and in aerobic environment or under the situation that catalase exists, through excised D-amino acid oxidase or in vitro expression of cells of D-amino acid oxidase, the D-glufosinate-ammonium is catalyzed to obtain 4-(hydroxymethylphosphinyl)-2-oxobutanoic; and under the action of glufosinate-ammonium dehydrogenase, the 4-(hydroxymethylphosphinyl)-2-oxobutanoic is catalyzed to generate the L-glufosinate-ammonium, the activity of a catalyst is improved by about 10 times, and the concentration of the substrate is increased by 5 times. The method is high in raw material conversion rate and high in yield, and products are easy to separate and purify.

The invention discloses a preparation method of R-3-aminobutyric acid. The method comprises the following steps: taking crotonic acid and ammonium salt as substrates; adding salt containing magnesiumions and regulating the pH (Potential of Hydrogen) by utilizing ammonia water; then adding recombinant aspartase as a biological enzyme catalyst; reacting at proper temperature and under an alkaline condition; after reacting, separating, purifying and crystallizing to obtain the R-3-aminobutyric acid. According to the method disclosed by the invention, ammonium ions of a reaction system are controlled, and reverse reaction is controlled and generation of byproducts is reduced; impurities including bacterial sludge, zymoprotein, sulfate ions, pigments and the like are intercepted by utilizing microfiltration and nanofiltration; high-quality products with high chiral purity and high impurity purity are obtained through continuous concentration and crystallization.

The invention relates to a (S)1-phenyl-1,2,3,4-tetrahydroisoquinoline synthesis method, wherein the reaction route is defined in the specification. The synthesis method comprises: 1) dissolving a rawmaterial 1 in a solvent, and adding an alkali and a catalyst; and (2) carrying out gas replacement by using hydrogen to form a hydrogen atmosphere, and carrying out an pressurization reaction to obtain (S)1-phenyl-1,2,3,4-tetrahydroisoquinoline 2, wherein the catalyst is a BIAMH system catalyst, a D-BIMAH system catalyst or a P-BIMAH system catalyst.

The invention discloses a high performance liquid chromatography detection analysis method for controlling a ticagrelor isomer, which employing a cellulose filler a chiral chromatographic column and takes lower paraffin hydrocarbon-lower alcohol as mobile phases, and the method can rapidly separate isomer impurity of ticagrelor.

This invention discloses a method for producing optical pure chiral mandelic acid. Use methyl benzoyl formate or the derivative of phenylglyoxylic acid as the bottom thing, the microorganism's cell of yeasts or mould whitlying etc. as catalyst, alternatively reduces the carbonyl in the bottom thing to the hydroxyl to get the chiral mandelic acid relying mainly on a kind of type (usually as R a type). This invention adopts chemistry-biology combination craft , with low cost, the chiral purity is high, easy to realize production for industrialization, it is easy to popularize and apply.

The invention discloses a method for synthesizing upadacitinib intermediate. The method includes performing a ring-forming reaction with 2-pentynoate and N-(methoxymethyl)-N-(trimethylsilylmethyl)benzylamine under a catalyst A to prepare a compound (3), by which the compound (7) or the compound (8) can be obtained in various manners. The method especially the first and the third technical schemes,is high in yield and purity and is high in overall yield. The method is simple in post-treatment and is suitable for industrial large-scale production.

The invention discloses a method for preparing furanone compounds, and provides a method for preparing a furanone compound III. The method includes the following steps that in a solvent, in the presence of inorganic salt, a compound II and a reducing agent are subjected to a reduction reaction, and the furanone compound III is obtained; the solvent is a fatty alcohol solvent or a mixed solvent of a fatty alcohol solvent and water. The brivaracetam can be prepared with the furanone compound III only with the three steps, and the synthetic route is short; the ee value of the compound II is larger than 99.0%, racemization does not occur in the reaction process, and the de value of a brivaracetam I crude product is larger than 99.0%; the brivaracetam I crude product is further purified through a crystal instead of a chirality high-pressure-liquid-phase preparing column, and the chirality purity of brivaracetam I can be further increased to be the de value of 99.80% or above; meanwhile, the content of other individual impurities of the brivaracetam I is smaller than 0.1%, and reaches the API level, and the method is suitable for industrial production. The formula is defined in the description.

The invention discloses a method for coproducing D-arginine and gamatine through a biological method. The method comprises the following steps: synthesizing arginine racemase genes argR according to a designed primer, establishing an expression vector pET24a-argR and transferring the expression vector into an Escherichia coli strain, thus obtaining genetically engineered bacteria of the arginine racemase; establishing an expression vector pET24a-speA through a speA gene segment amplified by PCR, transferring the expression vector pET24a-speA into Escherichia coli, thus obtaining L-arginine decarboxylase genetically engineered bacteria; stirring and reacting a transformation system containing L-arginine and a wet vector for expressing the genetically engineered bacteria of the arginine racemase, thus obtaining an enantiomer of L-arginine and D-arginine; removing the arginine racemase, supplementing a wet vector for expressing L-arginine decarboxylase, and continuously reacting until the L-arginine is completely consumed; and finally, separating and purifying the D-arginine and gamatine in the reaction system. The catalyzing enzyme activity is high, the separation and purification operations are simple, the raw materials are cheap, the total production cost is low; and the method has high conversion rate, can realize large-scale production and is suitable for industrial application.

The invention discloses a preparation method of an intermediate of a rosuvastatin calcium side chain, which comprises the following steps: firstly allowing (R)-4-cyano-3- methyl hydroxybutyrate to react with tert-butyl-dimethylchlorosilane, performing nitrile group hydrolysis in water phase under the action of an enzyme with the obtained (R)-4-cyano-3-tert-butyl dimethylsiloxyl methyl butyrate (II) as a substrate to generate a target product with a structure (I). The invention has the characteristics of being mild in reaction, high in yield, environment friendly, low in cost, and high in chiral purity of the prepared product.

The invention relates to a novel synthesis route of 1S,4R-1-methyl-4-(1-methylvinyl)-2-cyclohexene-1-ol. According to the preparation method, limonene is taken as the raw material, 1S,4R-1-methyl-4-(1-methylvinyl)-2-cyclohexene-1-ol is synthesized after four steps: epoxidation reactions, addition reactions, oxidation reactions, and elimination reactions, and the yield is high. The provided 1S,4R-1-methyl-4-(1-methylvinyl)-2-cyclohexene-1-ol preparation method has the advantages of high yield, high chiral purity product, low cost, environmental friendliness, and easy operation, and is suitablefor industrialization.

The invention aims to provide a preparation method for an alogliptin intermediate R-3-aminopiperidine dihydrochloride. The method has a brief route and is environmentally friendly and low-cost. A racemic compound 3- piperidine carboxamide which is cheap and easy to get is taken as a raw material, and is subjected to Hoffmann rearrangement reaction under the action of 1- fluoronaphthalene, hydrogen peroxide and fluoboric acid, which is similar to acid amides, to obtain 3-aminopiperdine which has one less carbon than the substrate. The method has simple reaction conditions, can be implemented at the room temperature, has simple operations in process, and is easy to monitor. The solvents are an ethanol-water mixture, and are low-cost and environmentally friendly. The carbon-lessened product 3-aminopiperidine is acidized and salified by concentrated hydrochloric acid. The hydrochloride is separated and salified by D-tartaric acid to obtain the target product R-3-aminopiperidine dihydrochloride. The chirality purity is high. The ee value is more than 99.5%. The reaction overall yield can reach 89%-93%. The cost is low. The method is suitable for industrial mass production.

The invention discloses a preparing method for a caproic acid derivative and provides a preparing method for a caproic acid derivative IV. The method comprises the following steps that in a polar nonprotic organic solvent, a compound III and an iodization reagent are subjected to a nucleophilic substitution reaction under inert gas shielding, and the caproic acid derivative IV is obtained. Brivaracetam can be prepared from the caproic acid derivative IV only through two steps, the synthetic route is short, the reaction condition is mild, posttreatment is simple, the reaction yield is high, and the production cost is low. Racemization does not happen in the reaction process, further purification is conducted through crystallization instead of a chiral high-pressure liquid phase preparing column, the chiral purity of the brivaracetam I can be improved till the de value is larger than 99.80%, meanwhile, the content of other single impurities of the brivaracetam I is smaller than 0.1%, the API level is reached, and the method is suitable for industrial production. The expression is shown in the description.

The invention discloses AHU-377alpha-phenethylamine salt polycrystalline type and a preparation method and application thereof and particularly discloses the AHU-377alpha-phenethylamine salt polycrystalline type. An X-radial powder diffraction pattern comprises peaks located at the diffraction angles 2 theta, namely 20.58 +/-0.2 degrees, 24.28 +/-0.2 degrees, 8.38 +/- 0.2 degrees and 23.20 +/-0.2 degrees or peaks located at the diffraction angles 2 theta, namely 23.28+/-0.2 degrees, 18.9+/-0.2 degrees, 13.7+/-0.2 degrees and 14.72+/-0.2 degrees. The AHU-377 is prepared into the phenethylamine salt to change the physicochemical properties of the AHU-377, such as crystallinity, solubleness and hygroscopicity. The AHU-377alpha-phenethylamine salt polycrystalline type and the preparation method and application thereof are mature in process and high in operability, the obtained product is high in quality, homogeneous and stable, chemical stability is achieved, storage is facilitated, and wide application prospect is achieved.

The invention discloses a method for synthesizing noradrenaline. The method comprises the steps that (-)-diisopinocampheyl chloroborane serves as a catalyst, a reducing agent is utilized in an ether solvent at a low temperature, particularly (-)-diisopinocampheyl chloroborane directly reduces noradrenaline to generate levarterenol, and a solution containing noradrenaline is obtained. According tothe method, levarterenol can be directly obtained by using (-)-diisopinocampheyl chloroborane as the reducing agent, resolution is not needed, and the cost and potential safety hazards are reduced simultaneously.

The invention relates to a polybasic nitrogen heterocyclic non-natural chiral amino acid and a synthesis method thereof. The amino acid can be applied to molecule building for antibiotic synthesis. According to the synthesis method, 2-aminodiethyl malonate and halogenated alkanes carry out substitution reactions, cyclization reactions, and decarboxylation reactions, and the reaction products are split to obtain the polybasic nitrogen heterocyclic non-natural chiral amino acid. The provided novel synthesis method has the advantages of simple synthesis route, low cost, convenient operation, andeasiness for commercial production, the chiral purity of obtained products is high, and the application prospect is good.