The invention relates to a device and a method for dynamically preparing heterocavity bacterium cellulose materials. The device comprises a thermometer hole, an acid liquor and alkali liquor adding hole, a handle, a venting hole, a pH meter hole, a nutrition supplement hole, a rotating shaft, a fermentation tank, a motor, a water bath device and a mould. The preparation method comprises the following steps of: inoculating bacterium cellulose strains into a fluid nutrient medium to be propagated and then transferring to a fermentation device provided with specific shape moulds for later dynamic disturbance cultivation, and then obtaining the heterocavity cellulose materials after the cultivation step. The size and the shape of the heterocavity BC (Bacterium Cellulose) materials prepared by utilizing the invention are controllable, and the moulds of the fermentation device can be detached to be reused. The preparation method has the advantages of simple and convenient implementation, low cost and high production efficiency, and is suitable for industrialization production. The heterocavity BC materials can be widely applied to cavity organ succedaneums of artificial vessels, nerve fiber conduits and the like, and can be used as food packing materials, such as meat sausage wrappers, jelly wrappers and the like.