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Device and method for dynamically preparing heterocavity bacterium cellulose materials

A bacterial cellulose and special-shaped technology, which is applied in the field of devices for dynamically preparing special-shaped cavity bacterial cellulose materials, can solve the problems of inability to carry out industrial production and application, long production cycle, and low production efficiency, and achieve good application prospects and production The effect of high efficiency and high crystallinity

Active Publication Date: 2010-12-15
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the disadvantages of the prior art methods are that specific oxygen permeable molds and static fermentation preparation are required, the production cycle is long, the production cost is high, the production efficiency is low, and industrial production and application cannot be carried out.

Method used

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  • Device and method for dynamically preparing heterocavity bacterium cellulose materials
  • Device and method for dynamically preparing heterocavity bacterium cellulose materials
  • Device and method for dynamically preparing heterocavity bacterium cellulose materials

Examples

Experimental program
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Effect test

Embodiment 1

[0051] 1. Design and assembly of fermentation equipment

[0052] 1.1 Design thinking

[0053] Since Acetobacterxylinum belongs to aerobic bacteria, it needs a lot of oxygen in the long-term fermentation and metabolism process. The growth of bacterial cellulose belongs to adsorption growth and must be supported by the medium. Therefore, the bacterial cellulose must be grown in a certain shape. , bacterial cellulose must be grown aerobically on a mold material of a specific shape. If the bacterial cellulose material to be prepared is a tubular material, stick-shaped, rod-shaped or tubular molds should be considered, not only to allow the bacterial cellulose to grow on these molds, but also to provide sufficient oxygen. For this reason, we have designed a A horizontal bioreactor equipped with a stick-shaped mold, which allows the mold to be slowly circulated and repeatedly submerged in the liquid medium for disturbed culture, so that the bacterial cellulose produced in the mediu...

Embodiment 2

[0062]As shown in Figure 1, a device for dynamically preparing bacterial cellulose materials with special-shaped cavities, a simple and efficient fermentation device for dynamically producing bacterial cellulose materials with special-shaped cavities, including a thermometer port 1, an acid and alkali liquid addition port 2, Handle 3, ventilation port 4, pH meter port 5, nutrient feeding port 6, rotating shaft 8, fermentation tank 9, motor 10, water bath device 11, mold, characterized in that: one end of the rotating shaft 8 is connected to the output shaft of the fixed motor 10 The other end of the rotating shaft 8 extends into the fermenting tank 9 from the center of one end of the fermenting tank 9; a mold is fixed on the rotating shaft 8 in the fermenting tank 9.

[0063] The material of mold 12 is selected from glass, pottery, purple sand, metal, silica gel, wood, cellulose, rubber, polyester, nylon, Orlon, polyvinyl alcohol, polyvinyl alcohol, polyester, Teflon, expanded ...

Embodiment 3

[0069] Such as figure 2 As shown, the difference between this embodiment and embodiment 2 only lies in:

[0070] (1) The shape of the mold is different. In the present embodiment, the mold is a glove-shaped mold 12, and the glove-shaped mold 12 is a solid or hollow structure. The cross-sectional shape of the glove-shaped mold 12 is circular, square, oval, triangle, heart shape or pentagram;

[0071] (2) In the device of the present embodiment, there is no need for the turntable to fix the glove-shaped molds, and only one or more glove-shaped molds need to be inserted sequentially on the rotating shaft.

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Abstract

The invention relates to a device and a method for dynamically preparing heterocavity bacterium cellulose materials. The device comprises a thermometer hole, an acid liquor and alkali liquor adding hole, a handle, a venting hole, a pH meter hole, a nutrition supplement hole, a rotating shaft, a fermentation tank, a motor, a water bath device and a mould. The preparation method comprises the following steps of: inoculating bacterium cellulose strains into a fluid nutrient medium to be propagated and then transferring to a fermentation device provided with specific shape moulds for later dynamic disturbance cultivation, and then obtaining the heterocavity cellulose materials after the cultivation step. The size and the shape of the heterocavity BC (Bacterium Cellulose) materials prepared by utilizing the invention are controllable, and the moulds of the fermentation device can be detached to be reused. The preparation method has the advantages of simple and convenient implementation, low cost and high production efficiency, and is suitable for industrialization production. The heterocavity BC materials can be widely applied to cavity organ succedaneums of artificial vessels, nerve fiber conduits and the like, and can be used as food packing materials, such as meat sausage wrappers, jelly wrappers and the like.

Description

technical field [0001] The invention belongs to the field of preparation of bacterial cellulose materials, in particular to a device and method for dynamically preparing special-shaped cavity bacterial cellulose materials. Background technique [0002] Bacterial Cellulose (BC for short) is a kind of pure cellulose produced by microorganisms. Bacterial cellulose, like plant fiber, is a straight chain formed by β-D-glucose through β-1,4-glucosidic bonds. The straight chains are parallel to each other, not in a helical conformation, and without branching structure, also known as β - 1,4-glucan. Bacterial cellulose has unique bioaffinity, biocompatibility and no allergic reaction, as well as high water holding capacity, degree of polymerization, crystallinity, good three-dimensional nanofiber network structure, high tension and strength, especially good Therefore, it has a wide range of uses in artificial blood vessels, tissue engineering scaffolds, artificial skin, and treatm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/34C12M1/04C12M1/02C12P19/04C12R1/02C12R1/01C12R1/41C12R1/38C12R1/025C12R1/05C12R1/065
CPCC12M29/20C12M41/26C12M27/06C12M41/12
Inventor 洪枫杨光杨雪霞
Owner DONGHUA UNIV
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