Method of dynamically preparing special-shaped cavity bacterial cellulose (BC) materials

A bacterial cellulose and cavity technology, which is applied in the field of dynamic preparation of special-shaped cavity bacterial cellulose materials, can solve problems such as inability to carry out industrial production and application, low production efficiency, and long production cycle, and achieve good application prospects and high production efficiency. High, low-cost effect

Inactive Publication Date: 2013-06-12
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, the disadvantages of the prior art methods are that specific oxygen permeable molds and static fermentation preparation are required, the production cycle is long, the production cost is high, the production efficiency is low, and industrial production and application cannot be carried out.

Method used

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  • Method of dynamically preparing special-shaped cavity bacterial cellulose (BC) materials
  • Method of dynamically preparing special-shaped cavity bacterial cellulose (BC) materials
  • Method of dynamically preparing special-shaped cavity bacterial cellulose (BC) materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1. Design and assembly of fermentation equipment

[0054] 1.1 Design thinking

[0055] Since Acetobacter xylinum is an aerobic bacterium, it needs a large amount of oxygen in the long-term fermentation and metabolism process. The growth of bacterial cellulose is an adsorption growth and must be supported by a medium. Therefore, it is necessary to let the bacterial cellulose grow in a certain shape To be formed, bacterial cellulose must be grown aerobically on a mold material of a specific shape. If the bacterial cellulose material to be prepared is a tubular material, stick-shaped, rod-shaped or tubular molds should be considered, not only to allow the bacterial cellulose to grow on these molds, but also to provide sufficient oxygen. For this reason, we have designed a A horizontal bioreactor equipped with a stick-shaped mold, which allows the mold to be slowly circulated and repeatedly submerged in the liquid medium for disturbed culture, so that the bacterial cellulo...

Embodiment 2

[0064] Such as Figure 1a , Figure 1b As shown, a device for dynamically preparing bacterial cellulose materials with special-shaped cavities, a simple and efficient fermentation device for dynamically producing bacterial cellulose materials with special-shaped cavities, including a thermometer port 1, an acid and alkali liquid addition port 2, a handle 3, Ventilation port 4, pH meter port 5, nutrient feeding port 6, rotating shaft 8, fermentation tank 9, motor 10, water bath device 11, mold, characterized in that: one end of the rotating shaft 8 is connected to the output shaft of the fixed motor 10, and the rotating shaft 8 The other end extends into the fermenter 9 from the central position of one end face of the fermenter 9; a mold is fixed on the rotating shaft 8 in the fermenter 9.

[0065] The material of mold 12 is selected from glass, pottery, purple sand, metal, silica gel, wood, cellulose, rubber, polyester, nylon, Orlon, polyvinyl alcohol, polyvinyl alcohol, polye...

Embodiment 3

[0071] Such as figure 2 As shown, the difference between this embodiment and embodiment 2 only lies in:

[0072] (1) The shape of the mold is different. In this embodiment, the mold is a glove-shaped mold 12, and the glove-shaped mold 12 is a solid or hollow structure. The cross-sectional shape of the glove-shaped mold 12 is circular, square, oval, triangle, heart shape or pentagram;

[0073] (2) In the device of this embodiment, there is no need for a turntable to fix the glove-shaped mold, and it is only necessary to insert more than one glove-shaped mold in sequence on the rotating shaft.

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Abstract

The invention relates to a method of dynamically preparing special-shaped cavity bacterial cellulose (BC) materials. According to the method of dynamically preparing special-shaped cavity BC materials, after a BC production bacterial strain is placed in a fluid nutrient medium for expanding cultivation, the BC production bacterial strain is transferred to a fermentation device provided with a specially shaped mould for dynamic disturbance cultivation, and the cavity special-shaped cellulose materials can be obtained after the cultivation. Compared with other physicochemical methods, the special-shaped cavity BC materials have the advantages of being unique in three-dimensional network nanostructure, high in chemical purity, high in degree of crystallinity, high in degree of polymerization, high in strength, good in biocompatibility, and the like. Besides, the method of dynamically preparing the special-shaped cavity BC materials is simple and feasible, low in cost, high in production efficiency, and suitable for large-scale industrial production.

Description

[0001] This application is a divisional application with application number 201010271720.3, application date 2010-09-02, and titled device and method for dynamically preparing special-shaped cavity bacterial cellulose materials. technical field [0002] The invention belongs to the field of preparation of bacterial cellulose materials, in particular to a method for dynamically preparing special-shaped cavity bacterial cellulose materials. Background technique [0003] Bacterial Cellulose (BC) is a kind of pure cellulose produced by microorganisms. Bacterial cellulose, like plant fiber, is a straight chain formed by β-D-glucose through β-1,4-glucosidic bonds. The straight chains are parallel to each other, not in a helical conformation, and without branching structure, also known as β -1,4-glucan. Bacterial cellulose has unique bioaffinity, biocompatibility and no allergic reaction, as well as high water holding capacity, degree of polymerization, crystallinity, good three-d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/02C12R1/01C12R1/41C12R1/38C12R1/025C12R1/05C12R1/065
Inventor 洪枫杨光杨雪霞
Owner DONGHUA UNIV
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