191results about How to "Low biocompatibility" patented technology

The present invention relate to a poly(vinyl alcohol) hydrogel construct having a wide range of mechanical strengths for use as a human tissue replacement. The hydrogel construct may include a tissue scaffolding, a low bearing surface within a joint, or any other structure which is suitable for supporting the growth of tissue.

An accommodating intraocular lens (IOL) can be implanted either alone or as part of a two-part lens assembly. The IOL comprises an optic, a flexible membrane and a peripheral edge coupling the optic and the flexible membrane. The peripheral edge comprises an external circumferential surface having a height and a force transmitting area defined along a portion of the height of the external circumferential surface. A closed volume spaces apart the optic and the flexible membrane. The optic is axially displaced and the flexible membrane changes in curvature about a central axis when a radial compressive force is applied to the force transmitting area. A volume defined by the closed volume remains fixed when the optic is axially displaced and the flexible membrane changes in curvature and / or when the radial compressive force is applied to the force transmitting area.

The present invention provides porous material of calcium phosphate of high strength whose open pores penetrate the porous body and have a size of 70 μm or more, preferably 100 μm or more, and are arranged in a three-dimensional network, whose porosity is sufficiently high for blood vessels to invade and perforate itself or for cells to infiltrate itself, whose chemical composition, in particular, Ca / P molar ratio can be freely changed within the range of 0.75 to 2.1, to which elements important for facilitating osteogenesis and producing resorbable effect can be added, and whose phase composition can be relatively easily changed. The invention is porous sintered compact of calcium phosphate which has artificially formed, penetrated open pores 70 μm to 4 mm in diameter, whose porosity is from 20% to 80%, and whose chief ingredient is calcium phosphate having a Ca / P molar ratio of from 0.75 to 2.1.

The invention discloses a macromolecular microcarrier and a preparation method thereof, belonging to the technical field of biomedicine. The preparation method comprises the following steps of: splitting a silk solution and a drug solution into micrometer grade liquid drops with core-shell structures under the action of a high-voltage electric field by adopting a coaxial high-voltage electrostatic technology and a freeze drying method; concreting through liquid nitrogen, and then preparing the microcarrier which is difficult to dissolve in water through freeze drying. The microcarrier is in the shape of a microsphere with the core-shell structure, and the diameter of the microsphere is 100-500 micrometers; the shell of the microcarrier comprises the components of silk fibroin; the random-coil conformation of silk fibroin molecules is 80-90 percent; the shell is in a porous structure, and an aperture is 5-20 micrometers; and a core of the microcarrier comprises the components of water-soluble drugs. The silk microcarrier has extensive application prospect in the fields of cell culture, drug release, and the like.

The invention relates to a porous material and a preparation method thereof, in particular to a technology in which silk fibroin is taken as a raw material to prepare porous three-dimensional materials. In the method, silk fibroin solution is injected into a metal mould, 1-24 hours of quick freezing is carried out on the solution at the low temperature of minus 10-minus 80 DEG C, thus obtaining afrozen body; the frozen body is preserved at the temperature of minus 5-minus 25 DEG C for 2-60 days, thus obtaining frozen crystalline solid; then the frozen crystalline solid is thawed and dried, thus obtaining the silk fibroin porous three-dimensional material. The silk fibroin of the porous material is fibroin I type crystal structure, therefore, the silk fibroin is insoluble in water and canbe completely degraded in a while and is finally degraded into nonpoisonous amino acid; the material of the invention has mutual run-through pores with aperture of 10-500 microns; the pores and pore diameter are relatively large, thus being suitable for growth of cells; meanwhile, in the process of preparation, no chemical cross linking agent, foaming agent or denaturing agent are needed, thus retaining fine biocompatibility of the silk fibroin.

The invention relates to a porous material and a preparation method thereof, and discloses a preparation method of a silk fibroin porous three-dimensional material. The preparation method comprises the following steps: sealing a silk fibroin aqueous solution for cultivation, then uniformly mixing with alcohol, freezing to directly obtain a water-insoluble silk fibroin porous bracket material, then regulating the secondary structure and the mechanical properties of the porous bracket by selectively using a post-treatment means, controlling the porosity, the aperture size and the like of the porous material by changing the concentration, the freezing temperature and other parameters of the silk fibroin, and finally removing the alcohol to obtain a pure silk fibroin porous bracket. The preparation process of the invention has the advantages of moderate conditions, no need of adding any crosslinking agent, hole-making agent and other toxic organic solvents, convenient control and easy industrialization, can effectively keep the biocompatibility of the silk fibroin, and can be used for repairing tissues such as bones, cartilages, ligaments, nerves, skins and the like and drug slow-releasing carriers and the like.

Disclosed herein is an injectable composition for the treatment of cancers comprising a poorly water-soluble anti-cancer drug, and glycofurol and Solutol HS15 as solubilizer, of the anticancer drug.

The invention relates to a stimuli-responsive composite material made from bacterial nano cellulose as well as a preparation method and application of the stimuli-responsive composite material. A bacterial nano cellulose base material in a hydrogel state is combined with polyelectrolyte macromolecules in an interpenetrating or semi-interpenetrating manner, and then is wholly or partially dehydrated to obtain the composite material. The composite material is high in rehydration and swelling capacities under the condition of a particular Ph, and keeps the characteristics of low swelling capacity and difficulty in rehydration under the condition of an off-design Ph value. The composite material has the characteristics of temperature response; the pore size of network of the composite material can be controlled, and the penetration rate of solute molecules can be controlled to release a drug in a control manner. Meanwhile, the composite material has the characteristics of high strength, high biocompatibility and the like of the bacterial nano cellulose, and can be applied to smart wound dressings, smart drug carriers, sensors, chemical valves and the like. The composite material has the advantages that the preparation process is simple, the cost is low, and a good application prospect is achieved.

The invention discloses a water-insoluble silk fibroin porous material and a preparation method thereof. The preparation method comprises the following steps of: adding one or more kinds of salt solution into silk fibroin solution, mixing the solution uniformly and then injecting the mixture into a metal die, freezing the mixture for 24 hours at the temperature of between 10 DEC below zero and 80 DEG C below zero, performing freeze drying on the mixture, and treating the mixture at the temperature of between 4 and 120 DEG C under the environment with the humidity of 30 to 98 percent to form the water-insoluble silk fibroin porous material. The solution loss rate of the water-insoluble silk fibroin porous material in 37 DEG C water for 24 hours is below 2 percent, and the material can be fully degraded into no-toxicity amino acid; the material has large holes and apertures, and is suitable for the growth of cells; and meanwhile, any chemical cross-linking agent, foaming agent or denaturing agent and the like does not need to be added during preparation, so good biocompatibility of the silk fibroin is kept, and the silk fibroin can be used for wound recovery materials for cell culture, skin, bones and gristles and medicinal release-controlled materials for temporary coverage, tissue isolation and hemostasis and the like.

The invention provides a preparation method of porous three-dimensional silk fibroin material, comprising the following steps of concentrating a silk fibroin solution, and manufacturing a bracket by the processed silk fibroin solution through a salting out method. By utilizing the self-assembling behaviors of silk fibroin, a secondary structure of the silk fibroin is improved. Compared with other methods of obtaining porous brackets, not only can the pore-forming property be good but also the inner walls of pores are in a very obvious fine structure.

The invention relates to a preparation method of a porous scaffold material and discloses a preparation method of a nano-fibrous silk fibroin-based porous scaffold. The preparation method comprises the following steps: blending a silk fibroin solution and a collagen solution under the temperature of 0-20 DEG C, standing to ensure the complete reaction of collagen and silk fibroin and generate nano-fiber; freezing the mixed solution to obtain a freezing body; performing freeze drying treatment on the freezing body to obtain a nano-fibrous porous scaffold material; and placing the nano-fibrous porous scaffold material in a vacuum drier with water and methanol, or an ethanol aqueous solution at the bottom, vacuumizing, and then standing to obtain the water-insoluble nano-fibrous porous scaffold. The obtained porous scaffold has 10-1000 microns of communicated pores. The pore walls consist of 10-100nm of nano-fibers; and the porous scaffold has similar nanoscale structures and micronscalestructures to extracellular matrix, is suitable for the adhesion and growth of cells and has good biocompatibility.

The invention discloses a preparation method of a silk fibroin aerogel. The silk fibroin aerogel is prepared from domestic silkworm silks, and a silk fibroin water solution is obtained by conducting degumming, dissolving and dialyzing; and the silk fibroin aerogel is prepared by the following steps: 1) concentrating the silk fibroin water solution by conducting room-temperature air blasting or thinning the silk fibroin water solution with the addition of de-ionized water, so that the concentration of the silk fibroin water solution is regulated to be 15-60mg / ml; 2) keeping the silk fibroin water solution in a container and conducting high-speed shearing on the silk fibroin water solution by virtue of a stirrer for 10-60min, so that a great amount of bubbles are formed and the silk fibroin water solution is converted into bubble-shaped liquid; and 3) freeze-drying the bubble-shaped liquid in a die, so that the silk fibroin aerogel is obtained. The aerogel prepared by the invention is quite low in density, and the aerogel has the advantages of being light in mass, high in specific surface area, good in biocompatibility and the like; the aerogel is especially applicable to such aspects as body liquid adsorption, biological dressings, sustained release of drugs, tissue repair and the like; and the aerogel has a broad prospect when used as a bio-medical material.

The present invention utilizes a combination of a porous carrier and an adsorbent such as a molecular sieve or an inorganic material selected from the group consisting of diatomaceous earth, glass powder or fibers, precipitated or fumed silica, kaolin and montmorillonite clays, and Ca exchanged permutites to make a more effective hemostatic device to treat wounds in mammalian animals. These hemostatic devices provide a lower heat rise to the skin as compared to direct application of zeolites to the skin.

The invention discloses a preparation method of alginate and silk fibroin composite sponge. Dried and sheared silkworm cocoons are degummed, fibroin fibers obtained after degumming is dissolved in a mixed solution including calcium chloride, ethanol and water, and a yellowish-brown solution is obtained. A pure silk fibroin solution is obtained with a dialysis and centrifugation method and mixed with a sodium alginate solution, the mixture is stirred, blended and freeze-dried, the dried product is immersed into a prepared solution (the mass ratio of calcium chloride to glycerin to ethanol being 7:3:90) and is taken out to be dried, and the composite sponge is obtained. The composite sponge is prepared from alginate and silk fibroin, and the application of the sponge material in actual production is expanded; compared with other sponge materials, the alginate and silk fibroin composite sponge has the excellent and outstanding characteristics of being economical, environment-friendly, good in biocompatibility and degradability and the like and can be applied to the fields such as medical dressing, cosmetic articles, architectural ornament and the like.

The invention discloses an injection gel containing submicron hyaluronic acid microspheres and a preparation method thereof. The diameter of each microsphere is 100 nanometers to 50 micrometers. Swelling ratio of the injection gel is 2-2000. The preparation method includes: dissolving biodegradable hyaluronic acid into organic solvent to obtain spinning solution; electrospinning the spinning solution using electrostatic spinning, and crosslinking through coagulating bath to obtain nano / submicron microspheres; dialyzing to purify the microspheres, preparing into injection with phosphate buffer or normal saline, and homogenizing with suspension of a stem cell growth factor system according to a certain volume ratio to obtain the injection gel. The injection gel containing submicron hyaluronic acid microspheres prepared by the method is fine in shaping performance, long in shape preservation, and fine in cell compatibility, and is applicable as therapeutic for ophthalmology and orthopedics, also applicable as tissue-engineered repair materials such as bone tissues, cartilage tissues, blood vessel tissues, heart tissues and nerve tissues or as filling material for plastic surgery.

The invention relates to a device and a method for dynamically preparing heterocavity bacterium cellulose materials. The device comprises a thermometer hole, an acid liquor and alkali liquor adding hole, a handle, a venting hole, a pH meter hole, a nutrition supplement hole, a rotating shaft, a fermentation tank, a motor, a water bath device and a mould. The preparation method comprises the following steps of: inoculating bacterium cellulose strains into a fluid nutrient medium to be propagated and then transferring to a fermentation device provided with specific shape moulds for later dynamic disturbance cultivation, and then obtaining the heterocavity cellulose materials after the cultivation step. The size and the shape of the heterocavity BC (Bacterium Cellulose) materials prepared by utilizing the invention are controllable, and the moulds of the fermentation device can be detached to be reused. The preparation method has the advantages of simple and convenient implementation, low cost and high production efficiency, and is suitable for industrialization production. The heterocavity BC materials can be widely applied to cavity organ succedaneums of artificial vessels, nerve fiber conduits and the like, and can be used as food packing materials, such as meat sausage wrappers, jelly wrappers and the like.

The invention relates to application of an immobilized quinone compound in accelerating the denitrification process of a microorganism, belonging to the technical field of chemical engineering, biological engineering and environmental engineering. The immobilized quinone compound is 1,8-dichloroanthraquinone and 1,5-dichloroanthraquinone or 1,4,5,8-tetrachloroanthraquinone. The immobilized quinone compound can intensify, regulate and control the denitrification process of an organism, accelerate the velocity and the capacity of the microorganism on the denitrification process of nitrogenous substances, effectively reduce the cost of water treatment and has the advantages of good stability, loss difficulty and reduced secondary pollution, low price of immobilized raw material sodium alginate, good biocompatibility, simple fixing process and low production cost and is easy to realize redox mediator recovery, regeneration and recycling.

A medical device including a silicone hydrogel which is less likely to harden due to drying, and which is less likely to cause stimulation at a site in contact with a human body, is disclosed. The medical device includes a silicone hydrogel satisfying the following conditions: (1) the water content in hydrous state is within the range of 10% by mass to 70% by mass; (2) the content of silicon atoms with respect to the total mass in dry state is within the range of 8% by mass to 30% by mass; and (3) the tensile elastic modulus in dry state is within the range of 0.1 MPa to 3.5 MPa.

Relating to the technical field of biological materials, the invention provides a preparation method of a silk fibroin three-dimensional porous material. The method includes: firstly subjecting a silk fibroin solution to freezing treatment, then conducting refrigeration treatment on a frozen sample, taking the sample out, performing thawing under room temperature condition to obtain a state wet three-dimensional porous material with stable structure, and conducting vacuum drying on the material by freeze-drying method so as to obtain a dried silk fibroin porous material. The technology can control the pore size, secondary structure composition, porosity and the like of the nano-porous material by changing the silk fibroin water solution concentration, freezing and refrigeration temperature, treatment time and other parameters. The preparation process involved in the invention is mild, has no need of any cross-linking agent, pore-forming agent or other toxic organic reagents, has green and controllable preparation conditions, and no waste residue, is green and pollution-free, and is easy for industrialization.

The invention belongs to the technical field of biology and relates to a nucleic acid self-assembled composite nanoflower particle material as well as a preparation method and application thereof. The self-assembled composite material refers to nanoflower particles formed by cocrystallizing contracted nucleic acid and Mg2P2O7, wherein the particle size of the particles is less than 500nm. The self-assembled composite material disclosed by the invention is of a nanoflower structure with high specific surface area, the DNA nanoflower self-assembled composite material has the yield close to 100% and the particle size of 500nm or less, and later utilization is facilitated; moreover, the DNA or RNA in the self-assembled composite material disclosed by the invention is an endogenous macromolecule in animal cells and has low toxicity and excellent biocompatibility, the DNA serves as a carrier of genetic information and can also achieve effects of targeting identification, enzyme catalysis and the like, multifunctional integration of the DNA nanoflower composite material can be realized by introducing functional nucleic acids, natural bio-molecules are totally adopted, and the self-assembled composite material is high in biocompatibility, low in cytotoxicity, low in production cost and favorable or large-scale production and development.

The invention relates to a bio-magnesium alloy with an anti-bacterial function and a method for manufacturing the bio-magnesium alloy by utilizing the laser selective smelting technology. The bio-magnesium alloy with the anti-bacterial function consists of a bio-magnesium alloy substrate and nanometer TiO2 distributed in the bio-magnesium alloy substrate; and in the bio-magnesium alloy with the anti-bacterial function, the mass percentage of the nanometer TiO2 is 3-7%. The manufacturing method comprises the steps that nanometer TiO2 powder and bio-magnesium alloy powder are prepared accordingto a designed ratio; the prepared powder is placed in a ball mill; in a protective atmosphere, mixed powder is obtained through high-speed ball milling; and then in the protective atmosphere, the bio-magnesium alloy with the anti-bacterial function is manufactured through laser selective smelting. The bio-magnesium alloy manufactured with the method has excellent anti-bacterial performance and cyto-compatibility, improved mechanical performance and a proper degradation rate; and the bio-magnesium alloy is advantaged compared with a current material when serving as an implant material.

The invention discloses hydrogel applicable to cell adhesion and a preparation method of the hydrogel. The hydrogel is formed by Ac-IIISLGK-NH2 and L-Glutamine (L-Gln) under the action of an enzymatic reaction, wherein the N-terminal of the Ac-IIISLGK-NH2 is acetylated, the C-terminal of the Ac-IIISLGK-NH2 is aminated, the mole ratio of the Ac-IIISLGK-NH2 to the L-Gln is 1:1, reaction temperature is 37-40 DEG C, and reaction time is 24-48 hours. The hydrogel has the advantages that the prepared hydrogel can allow cells to be well adhered to cell surfaces, is low in toxicity and good in biocompatibility, does not cause the non-specific immunity reaction of lymphocyte, is ideal nano tissue engineering material and is significant to human life health.

A method for producing an implant whose surface is roughened by the sandblast method using shot material containing fluoroapatite. Fluoroapatite, compared to hydroxyapatite, has poor biocompatibility, but is superior in hardness. It also has a property of being dissolved in acid. As a result, by the sandblast method using shot material containing fluoroapatite, the surface roughening is performed quite effectively, and shot materials remained on the surface can easily be removed by acid.

The invention relates to a copper-doped zinc oxide quantum dot nano antibacterial agent and a preparation method thereof. A chemical formula of the copper-doped zinc oxide quantum dot nano antibacterial agent is Zn<1-x>OCu<x> (x = 0.003-0.01). The preparation method comprises the following steps: weighing a zinc source and a copper source and performing dispersing in an organic solvent, performingstirring and heating in a water bath until a solution is clear and transparent, and performing stirring again to obtain a zinc and copper mixed precursor solution; adding an alkali source into an organic solvent, and performing stirring to obtain an alkali solution; dropwise adding the alkali solution into the zinc and copper mixed precursor solution, and performing stirring to obtain a mixed solution; and adding a precipitant into the mixed solution, performing stirring and then performing standing, washing and centrifuging to obtain the copper-doped zinc oxide quantum dot nano antibacterialagent. A size of the Cu-doped ZnO quantum dot is mainly distributed in 4-8 nm. A minimum bacteriostatic concentration of the nano antibacterial agent against Escherichia coli can reach 2.7 mg / mL, anda bacteriostatic rate against Escherichia coli can reach 81.79%.

The invention relates to a double-layer guided tissue regeneration membrane and a preparation method thereof. The double-layer guided tissue regeneration membrane is prepared by cross-linking a compact layer and a loose layer which are compounded together; the compact layer is prepared by blending polycaprolactone and chitosan and then adopting an electrostatic spinning method; and the loose layeris prepared by freeze-drying type I collagen. The method comprises the following steps: preparing a collagen gel solution from the type I collagen, and freeze-drying to obtain the loose layer; uniformly mixing the polycaprolactone solution with the chitosan solution to obtain a PCL / CS electrospinning solution, and preparing a compact layer on the surface of the loose layer by taking the PCL / CS electrospinning solution as a spinning solution through an electrostatic spinning method, so as to obtain a double-layer composite membrane; and crosslinking the double-layer composite membrane to obtain the double-layer guided tissue regeneration membrane. The double layers of the prepared material are combined more firmly, and the mechanical property is obviously improved; the compact layer is made of nanoscale superfine fibers, a fiber structure of an extracellular matrix is simulated, cell growth is facilitated, and a barrier effect can be effectively achieved.

The invention provides a biological tendon repairing material and a preparation method thereof. The biological tendon repairing material comprises an extracellular matrix body subjected to immunogen removal, wherein the extracellular matrix body consists of one or more layers and comprises a first surface and a second surface; the first surface comprises a first area and a second area; the mechanical strength of the first area is lower than that the second area. During preparation of an extracellular matrix subjected to immunogen removal, layering is avoided. As the biological tendon repairingmaterial is provided with the surface which consists of the first area and the second area, cellular infiltration of defective tissues is facilitated, tissue repairing is accomplished, stress born bythe repairing material can be uniformly distributed, and local stress cannot be excessively large. Besides, the surface has a high suture retention force and can serve as the second surface of a suture area of the repairing material, and the strength of the repairing material can be guaranteed while repairing of the defective tissues is facilitated.

The invention relates to a blood pump auxiliary device, in particular to an artificial heart auxiliary device which uses external magnetic field for driving a magnetic bearing to support an embeddable conical spiral impeller rotor, belonging to the field of biomedical engineering technique; the blood pump is arranged in an alternating magnetic field and comprises a small end shell (1) which is connected with a blood vessel, a large end shell (4) and a middle shell (3); the large end and the small end of the middle shell (3) are respectively connected with the small end shell (1) and the large end shell (4) hermetically; the small end shell (1) and the large end shell (4) are internally and fixedly provided with a small end guide wheel (2) and a large end guide wheel (6) with impellers on the external surface thereof respectively; the impeller rotor (5) is arranged in a space which is formed by the middle shell (3), the small end guide wheel (2) and the large end guide wheel (6); the impeller rotor (5) is internally provided with a shaft (11); a permanent magnetic strip (12) is fixedly arranged between the impeller rotor (5) and the shaft (11); and clearances are arranged between the impeller rotor (5) and the small end guide wheel (2) and the large end guide wheel (6). The blood pump auxiliary device effectively solves a series of technical problems such as sealing infection and biocompatibility and the like, which are caused by leads passing through skins and can not be overcome by traditional blood pumps.

Provided is an organic / inorganic hybrid-porous biodegradable polymer scaffold, which includes basic ceramic nanoparticles, an animal tissue or cell-derived bioactive first extracellular matrix material, and a biodegradable polymer and in which inner walls of pores are coated with a second extracellular matrix material.