Preparation method of silk fibroin porous three-dimensional material
A technology of silk fibroin and porous material, which is used in medical science, inactive components of polymer compounds, prostheses, etc. It can solve the problems of inability to adjust the structure ratio of silk fibroin I and silk fibroin II, and achieve the effect of good structure.
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[0037] Example 1:
[0038] Immerse about 50g of raw silk in 2L of 0.5%Na 2 CO 3 In the solution, stir and boil for 30 minutes, then remove and wash with deionized water. After repeating the above operation twice, the silk was dried at 60°C.
[0039] Weigh 15 g of the degummed silk after the above treatment, dissolve it in 100 ml of a BrLi solution with a concentration of 9.5 mol / L, and stir and dissolve at 60° C. for one hour. Then, a dialysis bag with a molecular weight cut-off of 3500 was used for dialysis with deionized water for four days to obtain an aqueous solution of silk protein with a concentration of 4%, and the concentration of the aqueous solution of silk protein was adjusted to 2%.
[0040] Take 10 mL of the above-mentioned silk protein solution, seal and cultivate at 60 ° C for 48 hours, and obtain an aqueous solution of silk fibroin nanospheres after incubation. The atomic force microscope diagram of the silk fibroin nanospheres is shown in figure 1 (Depend...
Example Embodiment
[0043] Example 2: Immerse about 50g of raw silk in 2L of 0.5% Na 2 CO 3 In the solution, stir and boil for 30 minutes, then remove and wash with deionized water. After repeating the above operation twice, the silk was dried at 60°C.
[0044] Weigh 15 g of the degummed silk after the above treatment, dissolve it in 100 ml of a BrLi solution with a concentration of 9.5 mol / L, and stir and dissolve at 60° C. for one hour. Then, a dialysis bag with a molecular weight cut-off of 3500 was used for dialysis with deionized water for four days to obtain an aqueous solution of silk protein with a concentration of 4%, and the concentration of the aqueous solution of silk protein was adjusted to 2%.
[0045] 10 mL of the above silk fibroin solution was taken, sealed and incubated at 60° C. for 48 hours, and an aqueous solution of silk fibroin nanospheres was obtained after incubation. Then, glycerin accounting for 60% of the mass of the porous scaffold in dry state was added, stirred e...
Example Embodiment
[0047] Embodiment three:
[0048] Immerse about 50g of raw silk in 2L of 0.5%Na 2 CO 3 In the solution, stir and boil for 30 minutes, then remove and wash with deionized water. After repeating the above operation twice, the silk was dried at 60°C.
[0049] Weigh 15 g of the degummed silk after the above treatment, dissolve it in 100 ml of a BrLi solution with a concentration of 9.5 mol / L, and stir and dissolve at 60° C. for one hour. Then, a dialysis bag with a molecular weight cut-off of 3500 was used for dialysis with deionized water for four days to obtain an aqueous solution of silk protein with a concentration of 4%, and the concentration of the aqueous solution of silk protein was adjusted to 2%.
[0050] 10 mL of the above silk fibroin solution was taken, sealed and incubated at 60° C. for 48 hours, and an aqueous solution of silk fibroin nanospheres was obtained after incubation.
[0051] Then, ethylene glycol, which accounts for 40% of the mass of the porous scaff...
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