Preparation method of silk fibroin porous three-dimensional material

Abstract

The invention relates to a porous material and a preparation method thereof, and discloses a preparation method of a silk fibroin porous three-dimensional material. The preparation method comprises the following steps: sealing a silk fibroin aqueous solution for cultivation, then uniformly mixing with alcohol, freezing to directly obtain a water-insoluble silk fibroin porous bracket material, then regulating the secondary structure and the mechanical properties of the porous bracket by selectively using a post-treatment means, controlling the porosity, the aperture size and the like of the porous material by changing the concentration, the freezing temperature and other parameters of the silk fibroin, and finally removing the alcohol to obtain a pure silk fibroin porous bracket. The preparation process of the invention has the advantages of moderate conditions, no need of adding any crosslinking agent, hole-making agent and other toxic organic solvents, convenient control and easy industrialization, can effectively keep the biocompatibility of the silk fibroin, and can be used for repairing tissues such as bones, cartilages, ligaments, nerves, skins and the like and drug slow-releasing carriers and the like.

Description

technical field

[0001] The invention relates to a porous material and a preparation method thereof, in particular to a technique for preparing a porous three-dimensional material using silk fibroin as a raw material. The prepared material can be applied to technical fields such as biomedicine, biotechnology, and tissue engineering. Background technique

[0002] Biomedical materials, especially tissue engineering scaffold materials, tissue repair regeneration materials, etc., require the materials used not only to have good biocompatibility, but also to have a suitable three-dimensional porous structure, controllable biodegradability, and certain mechanical strength. , and the ability to release factors or other related drugs.

[0003] For the method of preparing biomedical materials, especially the method of preparing porous scaffolds of biomaterials, it is required that the preparation process should be as gentle as possible, and the use of toxic organic solvents should be ...

Examples

Embodiment 1

[0038] Soak about 50g raw silk in 2L 0.5% Na 2 CO 3 solution, stirred and boiled for 30 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0039] 15 g of the degummed silk after the above treatment was weighed and dissolved in 100 ml of BrLi solution with a concentration of 9.5 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut-off of 3500 to dialyze with deionized water for four days to obtain a silk protein aqueous solution with a concentration of 4%, and adjust the concentration of the silk protein aqueous solution to 2%.

[0040] Take 10 mL of the above-mentioned silk protein solution and incubate in a sealed manner at 60° C. for 48 hours. After incubation, an aqueous solution of silk fibroin nanospheres is obtained. The atomic force microscope image of the silk fibroin nanospheres is shown in figure 1 (Depend on figure 1 It can be seen tha...

Embodiment 2

[0043] Embodiment two: about 50g raw silk is immersed in 2L 0.5%Na 2 CO 3 solution, stirred and boiled for 30 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0044] 15 g of the degummed silk after the above treatment was weighed and dissolved in 100 ml of BrLi solution with a concentration of 9.5 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut-off of 3500 to dialyze with deionized water for four days to obtain a silk protein aqueous solution with a concentration of 4%, and adjust the concentration of the silk protein aqueous solution to 2%.

[0045] Take 10 mL of the above silk protein solution, and incubate in a sealed manner at 60° C. for 48 hours, and obtain an aqueous solution of silk fibroin nanospheres after incubation. Then add glycerin accounting for 60% of the mass of the porous scaffold in a dry state, stir evenly, pour into a po...

Embodiment 3

[0048] Soak about 50g raw silk in 2L 0.5% Na 2 CO 3 solution, stirred and boiled for 30 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0049] 15 g of the degummed silk after the above treatment was weighed and dissolved in 100 ml of BrLi solution with a concentration of 9.5 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut-off of 3500 to dialyze with deionized water for four days to obtain a silk protein aqueous solution with a concentration of 4%, and adjust the concentration of the silk protein aqueous solution to 2%.

[0050] Take 10 mL of the above silk protein solution, and incubate in a sealed manner at 60° C. for 48 hours, and obtain an aqueous solution of silk fibroin nanospheres after incubation.

[0051] Then add ethylene glycol accounting for 40% of the mass of the porous scaffold in a dry state, stir evenly, pour into a polyethy...

technical field

[0001] The invention relates to a porous material and a preparation method thereof, in particular to a technique for preparing a porous three-dimensional material using silk fibroin as a raw material. The prepared material can be applied to technical fields such as biomedicine, biotechnology, and tissue engineering. Background technique

[0002] Biomedical materials, especially tissue engineering scaffold materials, tissue repair regeneration materials, etc., require the materials used not only to have good biocompatibility, but also to have a suitable three-dimensional porous structure, controllable biodegradability, and certain mechanical strength. , and the ability to release factors or other related drugs.

[0003] For the method of preparing biomedical materials, especially the method of preparing porous scaffolds of biomaterials, it is required that the preparation process should be as gentle as possible, and the use of toxic organic solvents should be ...

Examples

Embodiment 1

[0038] Soak about 50g raw silk in 2L 0.5% Na 2 CO 3 solution, stirred and boiled for 30 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0039] 15 g of the degummed silk after the above treatment was weighed and dissolved in 100 ml of BrLi solution with a concentration of 9.5 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut-off of 3500 to dialyze with deionized water for four days to obtain a silk protein aqueous solution with a concentration of 4%, and adjust the concentration of the silk protein aqueous solution to 2%.

[0040] Take 10 mL of the above-mentioned silk protein solution and incubate in a sealed manner at 60° C. for 48 hours. After incubation, an aqueous solution of silk fibroin nanospheres is obtained. The atomic force microscope image of the silk fibroin nanospheres is shown in figure 1 (Depend on figure 1 It can be seen tha...

Embodiment 2

[0043] Embodiment two: about 50g raw silk is immersed in 2L 0.5%Na 2 CO 3 solution, stirred and boiled for 30 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0044] 15 g of the degummed silk after the above treatment was weighed and dissolved in 100 ml of BrLi solution with a concentration of 9.5 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut-off of 3500 to dialyze with deionized water for four days to obtain a silk protein aqueous solution with a concentration of 4%, and adjust the concentration of the silk protein aqueous solution to 2%.

[0045] Take 10 mL of the above silk protein solution, and incubate in a sealed manner at 60° C. for 48 hours, and obtain an aqueous solution of silk fibroin nanospheres after incubation. Then add glycerin accounting for 60% of the mass of the porous scaffold in a dry state, stir evenly, pour into a po...

Embodiment 3

[0048] Soak about 50g raw silk in 2L 0.5% Na 2 CO 3 solution, stirred and boiled for 30 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0049] 15 g of the degummed silk after the above treatment was weighed and dissolved in 100 ml of BrLi solution with a concentration of 9.5 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut-off of 3500 to dialyze with deionized water for four days to obtain a silk protein aqueous solution with a concentration of 4%, and adjust the concentration of the silk protein aqueous solution to 2%.

[0050] Take 10 mL of the above silk protein solution, and incubate in a sealed manner at 60° C. for 48 hours, and obtain an aqueous solution of silk fibroin nanospheres after incubation.

[0051] Then add ethylene glycol accounting for 40% of the mass of the porous scaffold in a dry state, stir evenly, pour into a polyethy...

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