41 results about "Repairing tissues" patented technology

Non-woven graft materials for use in specialized surgical procedures such as neurosurgical procedures, methods for making the non-woven graft materials, and methods for repairing tissue such as neurological tissue using the non-woven graft materials are disclosed. More particularly, disclosed are non-woven graft materials including at least two distinct fiber compositions composed of different polymeric materials, methods for making the non-woven graft materials and methods for repairing tissue in an individual in need thereof using the non-woven graft materials.

Provided are tissue repair and sealing devices, and methods for the use of tissue repair and sealing devices, for use in both minimally invasive surgical (MIS) procedures and open, non-MIS procedures to rapidly repair tissue fenestrations and create a pressure-resistant, watertight seal in a tissue barrier. Tissue repair and sealing devices disclosed herein comprise an integrated graft and deployable clasp assembly and an applicator assembly having a graft-clasp lock cylinder that is slidably connected to a folded, deployable clasp. The applicator assembly places a graft on a tissue inner surface and a deployable clasp on a tissue outer surface to secure the graft to the tissue inner surface to, thereby, repair a tissue fenestration and create a watertight barrier.

The invention relates to a tissue engineering articular cartilage simulating a four-layer structure of a natural cartilage and an in-situ 3D printing method of the tissue engineering articular cartilage, the structure and component characteristics of'surface layer-transitional layer-radiation layer-calcification layer 'of the natural cartilage are re-carved through precise layered modeling and layer-specific bio-ink, and the articular cartilage is obtained by means of an optimized in-situ 3D printing process. The tissue engineering cartilage which is matched with dissection of a defect area and consistent with mechanical and physiological functions of natural cartilage is directly constructed in an articular cavity. Besides, the in-vitro induced differentiation step of the BMSCs is reduced, the risk of cell mutation is reduced, meanwhile, the long-term integration of the repaired tissue and the host cartilage is improved, and the problem that the long-term effectiveness of clinical repair is insufficient is solved.

Particles of non-woven graft materials for use in specialized surgical procedures such as soft tissue repair and wound management procedures, methods for making the powder, and methods for repairing tissue such as neurological tissue using the powder are disclosed. The particles can advantageously be used to fill irregular shaped areas or can be used in conjunction with non-woven graft materials.

A suturing apparatus may include a shaft member having a lumen, and a first jaw member and a second jaw member that move between an open configuration and a closed configuration. A distal portion of the first jaw member may be pivotably connected to a distal portion of the second jaw member, and the distal portion of the second jaw member may have a distal opening. The suturing apparatus may further include a needle translates through the lumen of the shaft member and carries a suture, and a retention member in the distal opening of the second jaw member to remove the suture from the needle. The suture may be wrapped around the distal portion of the second jaw member. The suturing apparatus may be included in a suturing system having a suture. The suturing apparatus may be used in a method of repairing tissue.

Described herein are methods for repairing tissues (e.g., a meniscus, anterior cruciate ligament, rotator cuff, etc.) using collagen implants suitable. For example, the collagen implants are injected into a repair site to heal the tissue. Further described are collagen slurries, injectable collagen implants and methods of preparation thereof.

The invention discloses a bio-responsive high polymer material and application thereof, and belongs to the technical field of biomedical materials. The bio-responsive high-molecular material provided by the invention contains polymers shown as formulas i to viii. Wherein the polymers of the formulas i to viii have excellent fluorescence performance, aggregation-induced fluorescence enhancement performance, multicolor luminescence performance and biological response capability based on a redox reversible switch, and can be applied to sensing, counterfeiting prevention, metal ion detection, multicolor imaging of in-vivo and in-vitro cells and / or tissues and quantitative detection of in-vivo and in-vitro oxidized and / or reducing substances; active oxygen in in-vivo and in-vitro tissues and cells can be removed to achieve long-acting anti-inflammatory and anti-oxidation effects, and the compound can be used for treatment of oxidative stress related diseases and visual monitoring of the treatment process; the biological activity of enhancing tissue retention, stimulating collagen generation, repairing tissue and resisting oxidation and aging of the tissue is achieved, and the effect of filling and repairing in-vivo cells and tissue can be effectively improved; the compound also has the capability of being chelated with metal ions to form a high-molecular metal complex, and can be used for resisting tumors, resisting bacteria, filling and repairing tissues, promoting collagen generation and the like.

The invention relates to stimuli-responsive hydrogel for repairing tissue damage and a preparation method of the stimuli-responsive hydrogel, and the hydrogel is prepared from the following components in parts by weight: 6 to 8 parts of modified isocyano ethyl methacrylate, 2 to 4 parts of modified chitosan, 0.2 to 0.3 part of N, N '-methylene bisacrylamide, 0.1 to 0.2 part of ammonium persulfate and 3 to 5 parts of deionized water. The preparation method comprises the following steps: dissolving modified isocyano ethyl methacrylate and modified chitosan in deionized water, uniformly stirring to prepare a solution, adding N, N '-methylenebisacrylamide and ammonium persulfate, uniformly stirring by using a magnetic stirrer, transferring the mixed solution into a closed reaction container, introducing nitrogen for 10-15 minutes, putting the reaction container into a water bath at 60-80 DEG C, reacting for 30-60 minutes, filtering, washing, and drying to obtain the modified chitosan / isocyanatoethyl methacrylate copolymer. The stimuli-responsive hydrogel for tissue damage repair is obtained. The hydrogel disclosed by the invention has a multi-stimulation response controlled release mechanism and good dynamic network self-healing performance.

Particles of non-woven graft materials for use in specialized surgical procedures such as soft tissue repair and wound management procedures, methods for making the powder, and methods for repairing tissue such as neurological tissue using the powder are disclosed. The particles can advantageously be used to fill irregular shaped areas or can be used in conjunction with non-woven graft materials.

Methods of repairing tissue include obtaining a suture including a first end defining an opening and a second end capable of passing through the opening; passing the first end through the tissue at a first location; passing the second end through the tissue at a second location; forming a luggage tag configuration by passing the second end through the first end; and securing the luggage tag configuration against the tissue. In some examples, the suture is a continuous closed loop, and in others the suture has a barbell configuration, with a linear central portion and a loop at each end. In still examples, the suture has a loop at one end and a tail at the other. The suture may be used without an anchor or may be part of a suture assembly including a suture anchor and, optionally, at least one other suture.

This invention belongs to the field of renal pathology testing technology and discloses a method for detecting type IV collagen expression in renal biopsy tissue, comprising the following steps: dewaxing and hydrating paraffin sections of renal biopsy tissue to obtain processed tissue sections; adding the processed tissue sections to a repair solution, heating to boiling, and then cooling to room temperature to obtain repaired tissue sections; adding protease to the repaired tissue sections for enzymatic digestion to obtain enzymatically digested tissue sections; adding a primary antibody to the enzymatically digested tissue sections for preliminary incubation, followed by adding a fluorescein-labeled secondary antibody against the primary antibody for further incubation. This method for detecting type IV collagen expression in renal biopsy tissue is applicable to cases where renal biopsy tissue lacks glomeruli or has too few glomeruli to detect type IV collagen expression. It has high specificity and can provide a new detection method for Alport syndrome in cases where renal biopsy tissue lacks glomeruli.

The invention provides a polypeptide PN-VW22 and application thereof, and belongs to the technical field of polypeptide drugs. The polypeptide PN-VW22 provided by the invention is specifically as follows A1) or A2): A1) an amino acid sequence as shown in SEQ ID NO.1; a2) a fusion protein obtained by connecting a label to the N end or / and C end of A1). The polypeptide has obvious cell proliferation activity and anti-staphylococcus aureus bifunctional activity, and also has a wound repairing effect. The polypeptide is small in molecular weight and stable in structure, can be industrially produced through solid-phase chemical synthesis, can also be industrially applied to preparation of treatment medicines for promoting skin wound repair, and can be used as a candidate polypeptide medicine or a medical beauty product raw material for treating body surface wounds, repairing tissues, burns and skin ulcers, reducing scars and accelerating scar repair.

The invention discloses a traditional Chinese medicine concentrated solution for cell energy activation type scoliosis and a preparation process, and relates to the technical field of traditional Chinese medicine processing. The traditional Chinese medicine concentrated solution is prepared from the following raw materials in parts by weight: 100-140 parts of radix dipsaci, 80-120 parts of cortex eucommiae, 130-170 parts of radix astragali, 60-100 parts of radix angelicae sinensis, 50-70 parts of rhizoma chuanxiong, 60-80 parts of radix angelicae pubescentis, 80-100 parts of fructus lycii, 40-60 parts of radix glycyrrhizae and 15-25 parts of activation auxiliary materials. Himalayan teasel root and eucommia ulmoides in the formula are core components for strengthening muscles and bones and can enhance the toughness of ligaments and tendons around the spine; radix astragali can tonify qi, angelica sinensis can nourish blood, local blood circulation of the spine can be improved, and nutrition is provided for tissue repair; ligusticum wallichii can promote blood circulation to remove meridian obstruction, radix angelicae pubescentis can expel wind-damp, and inflammation and adhesion of soft tissues around The Chinese wolfberry fruits can nourish the liver and the kidney, the licorice roots are matched to harmonize all the medicines, the added activating auxiliary material semen cuscutae extract can activate energy metabolism of osteoblasts and chondrocytes around the spine in a targeted mode, collagen synthesis and calcium deposition are promoted, and tissue damage related to spine malformation is repaired from the cellular level.

An implantable prosthesis for repairing tissue defects includes a layer of repair fabric and a plurality of microtextured grip segments mounted to the repair fabric. The grip segments include a pattern of perforations on a tissue facing surface to promote tissue ingrowth.

The application relates to a method for inducing mesenchymal stem cells to differentiate into chondroblasts and a composition for promoting regeneration of articular cartilage and application, and belongs to the technical field of biomedical materials. The method for inducing mesenchymal stem cells to differentiate into chondroblasts comprises the following steps: inoculating and culturing on a substrate, wherein the surface of the substrate is provided with a plurality of convex blocks, the plurality of convex blocks are arranged at equal intervals along any extension direction of the surface of the substrate; the height of the convex blocks is not greater than 2 mu m, the ratio of the width of the convex blocks to the interval between adjacent two convex blocks is 1:0.8-1.2, and the interval between the adjacent two convex blocks is not less than 25 mu m; the method further comprises the following steps: spreading mesenchymal stem cells to the surface of the substrate for culture to obtain mesenchymal stem cells with mechanical memory of chondroblast differentiation; and the surface of the substrate comprises the surface of the convex blocks and the surface of the concave parts between the convex blocks. The method can promote the chondroblast differentiation of mesenchymal stem cells, make the subsequently formed repair tissue more close to the original cartilage, and has the advantages of simple operation, high cell survival rate and stable effect.

Provided herein are methods of modulating cellular stress pathways using small molecule steroid receptor coactivators (SRC) stimulators. A method of repairing tissue in a subject comprises administering to the subject an effective amount of a pharmaceutical composition comprising a compound as described herein. Methods of protecting tissue adjacent to an injury site or reducing tissue loss in a subject, preserving insulin production and preventing diabetes in a subject, and / or inhibiting kidney fibrosis in a subject are further provided herein.

Non-woven graft materials for use in specialized surgical procedures such as neurosurgical procedures, methods for making the non-woven graft materials, and methods for repairing tissue such as neurological tissue using the non-woven graft materials are disclosed. More particularly, disclosed are non-woven graft materials including at least two distinct fiber compositions composed of different polymeric materials, methods for making the non-woven graft materials and methods for repairing tissue in an individual in need thereof using the non-woven graft materials.

The application provides a tissue staining method based on antigen repair treatment combined with silver staining, comprising the following steps: a) high-temperature heat repair of a tissue section to be treated with an antigen repair solution, and washing with water after cooling to obtain a repaired tissue section; b) silver staining of the repaired tissue section obtained in step a), dehydration, and then mounting to obtain a stained tissue section. Compared with the prior art, the tissue staining method based on antigen repair treatment combined with silver staining provided by the application adopts specific process steps and conditions to realize overall good interaction: first, the brain tissue is subjected to antigen repair treatment, and then the silver staining technology is adopted, which significantly improves the signal-to-noise ratio of the senile plaque region, and ideal staining effect is obtained, which can effectively improve the detail visibility in pathological detection, help more accurately evaluate the morphology and distribution characteristics of the senile plaque, and has a wide application prospect.

Provided are tissue repair and sealing devices, and methods for the use of tissue repair and sealing devices, for use in both minimally invasive surgical (MIS) procedures and open, non-MIS procedures to rapidly repair tissue fenestrations and create a pressure-resistant, watertight seal in a tissue barrier. Tissue repair and sealing devices disclosed herein comprise an integrated graft and deployable clasp assembly and an applicator assembly having a graft-clasp lock cylinder that is slidably connected to a folded, deployable clasp. The applicator assembly places a graft on a tissue inner surface and a deployable clasp on a tissue outer surface to secure the graft to the tissue inner surface to, thereby, repair a tissue fenestration and create a watertight barrier.

Compositions and methods for treatment of burns and other wounds. A unitary product containing all ingredients of the compositions may deliver composition ingredients to a surface of a burn / wound treatment site. The unitary product may include an ointment. The unitary product may include a cream. The unitary product may include a hydrogel. The compositions may include antibiotic ethacridine lactate. The compositions may include metal-containing species. The compositions may include a moisture-modulating agent. The compositions, when applied to the site, may constitute a barrier overlying the surface. The unitary product may deliver the antibiotic onto the surface. The unitary product may deliver the metal-containing species onto the surface. Each of the antibiotic and the metal-containing species may positively modulate wound bed and perilesional cellular processes supportive of healing. Initial testing and use studies have yielded good wound closures, with minimal-to-no scarring and high regain of function of repaired tissues.

A suturing apparatus may include a shaft member having a lumen, and a first jaw member and a second jaw member that move between an open configuration and a closed configuration. A distal portion of the first jaw member may be pivotably connected to a distal portion of the second jaw member, and the distal portion of the second jaw member may have a distal opening. The suturing apparatus may further include a needle translates through the lumen of the shaft member and carries a suture, and a retention member in the distal opening of the second jaw member to remove the suture from the needle. The suture may be wrapped around the distal portion of the second jaw member. The suturing apparatus may be included in a suturing system having a suture. The suturing apparatus may be used in a method of repairing tissue.

Technologies are disclosed for an implant device that may be configured to repair tissue. The device may include a distal end configured to be connectable to at least a first part of the tissue. The device may include a proximate end configured to be connectable to at least a second part of the tissue. The device may include a substrate configured to form a connection between the first part of the tissue and the second part of the tissue. The device may include a longitudinal axis that may extend between the distal end to the proximate end within the substrate. The device may include at least one preformed channel in the substrate. The device may include at least one suture preloaded at least partially within the at least one channel. The at least one preformed channel may be formed during and / or proximate to a formation of the device.

The present invention is a multi-stage treatment that heals tissue or organ damage (e.g., linear defects, fissures, and fibrillations, as well as focal and large defects) in collagen-rich tissues and organs such as articular cartilage. The present invention includes methods 1) to prime tissues in preparation for treatment, which comprises “melting” the tissue matrix, 2) to add or fill the damaged area with a “melding” agent, comprising of endogenous or exogenous tissue matrix, with or without cells, with or without exogenous biomaterials, and with or without endogenous or exogenous enzymes, such that the melding agent enhances anchoring into the defect for the purpose of integration and / or tissue healing. The Melt-and-Meld process can also be applied in conjunction with any existing treatments of tissue or organ defects.

The application provides application of a lentivirus vector overexpressing CYGB in repairing engineering materials. Specifically relates to application of the lentivirus vector overexpressing CYGB in repairing tissue defect engineering materials, and application of adipose-derived stem cells containing the lentivirus vector overexpressing CYGB in repairing tissue defect engineering materials. The lentivirus vector overexpressing CYGB is used to transfect adipose-derived stem cells, and the obtained adipose-derived stem cells containing the lentivirus vector overexpressing CYGB can promote proliferation and adipogenic differentiation of the adipose-derived stem cells, form tissue engineering fat, and be filled into a tissue defect site as an implant material to repair the tissue defect.

The invention provides a material omics strategy combining bioinformatics and mathematical modeling. The material omics strategy is used for regulating extracellular matrix-based hydrogel. The prepared improved material can be particularly used for treating tissue defect diseases such as tendon defects. The hydrogel can be directly used as a transplantation material and other treatment means, and the provided hydrogel can also be indirectly applied to preparation of differentiated stem cells, cell-derived extracellular matrix products or extracellular vesicles. In addition, the invention also provides a preparation method of the hydrogel, the transplantation material, the differentiated stem cell, the extracellular matrix and the vesicle product, and a method for repairing tissue defects by using the materials.

Systems, methods, and kits are described for repairing a fibrocartilage defect in a subject. The fibrocartilage defect is contacted with a first composition containing an oxidized and methacrylated glycosaminoglycan to form an imine bond between the glycosaminoglycan and the fibrocartilage defect, thereby coating the fibrocartilage defect with the glycosaminoglycan. The fibrocartilage defect coated with the glycosaminoglycan is then contacted with a mixture of a pre-polymer hydrogel composition containing a first crosslinking unit that, when polymerized, is capable of bonding to methacrylate and a hydrogel polymerization initiator composition, thereby forming a hydrogel that is covalently bonded to the glycosaminoglycan through methacrylate.

Non-woven graft materials for use in specialized surgical procedures such as neurosurgical procedures, methods for making the non-woven graft materials, and methods for repairing tissue such as neurological tissue using the non-woven graft materials are disclosed. More particularly, disclosed are non-woven graft materials including at least two distinct fiber compositions composed of different polymeric materials, methods for making the non-woven graft materials and methods for repairing tissue in an individual in need thereof using the non-woven graft materials.

Described herein are methods for repairing tissues (e.g., a meniscus, anterior cruciate ligament, rotator cuff, etc.) using collagen implants suitable. For example, the collagen implants are injected into a repair site to heal the tissue. Further described are collagen slurries, injectable collagen implants and methods of preparation thereof.

Described herein are methods for repairing tissues (e.g., a meniscus, anterior cruciate ligament, rotator cuff, etc.) using collagen implants suitable. For example, the collagen implants are injected into a repair site to heal the tissue. Further described are collagen slurries, injectable collagen implants and methods of preparation thereof.