Preparation method of secondary structure controllable silk fibroin porous material with no need of organic solvent

A technology of silk fibroin and porous materials, which is applied in the fields of pharmaceutical formula, medical science, prostheses, etc. It can solve the problems of unfavorable large-scale production, harmful environment in the preparation process, slow degradation, etc., and achieve good porosity and non-toxicity Side effects, easily modifiable effects

Inactive Publication Date: 2016-07-13
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] In view of this, the purpose of the present invention is on the basis of the traditional freeze-drying method, by regulating the structure of silk protein, to provide a kind of porous silk fibroin material with variable secondary structure that does not require organic solvents and other sacrifices. preparation method, thereby realizing the change and adjustment of silk protein cytocompatibility, mechanical properties and degradation performance, and overcoming the high hardness, slow degradation, harmful environment and unfavorable The defects of large-scale production, the resulting product has good biodegradability, high porosity, adjustable crystal structure, excellent biomimetic properties, and has better performance in promoting tissue repair, especially soft tissue repair

Method used

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  • Preparation method of secondary structure controllable silk fibroin porous material with no need of organic solvent
  • Preparation method of secondary structure controllable silk fibroin porous material with no need of organic solvent
  • Preparation method of secondary structure controllable silk fibroin porous material with no need of organic solvent

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Prepare silk fibroin solution. Specifically: put 60g silk in 0.02M Na 2 CO 3 Boil in the solution at 100°C for 30 minutes to remove sericin on the outside of the silk, rinse with deionized water, repeat the above operation 3 times, and then dry the silk at 60°C. Weigh 27 g of the degummed silk after the above treatment and dissolve in 100 mL of 9.3 mol / LLiBr solution, and dissolve at 60° C. for 4 hours. Then use a dialysis bag with a molecular weight cut-off of 3500 to immerse in deionized water and dialyze for 3 days, during which the water is changed every two hours to remove LiBr in the solution, thereby obtaining a pure silk fibroin solution with a concentration of 6%.

[0031] After the silk fibroin solution was placed at -4°C for 48 hours, it was then frozen at -2°C for 24 hours. After being put into a lyophilizer and dried for 48 hours, the nano silk protein porous material was obtained.

[0032] See figure 2 , figure 2 It is an electron micrograph of the...

Embodiment 2

[0034] A pure silk fibroin solution with a concentration of 6% was prepared by the same method as in Example 1.

[0035] After the silk fibroin solution was placed at -20°C for 48 hours, it was then frozen at -4°C for 24 hours. After being put into a lyophilizer and dried for 48 hours, the nano silk protein porous material was obtained.

[0036] See image 3 , image 3 It is the electron micrograph of the silk protein scaffold obtained by the salting-out method after vacuum drying of the concentrated silk fibroin solution described in Example 2. It can be seen from the figure that the pore diameter of the above scaffold is about 188.35±19.41 μm.

Embodiment 3

[0038] A pure silk fibroin solution with a concentration of 6% was prepared by the same method as in Example 1.

[0039] The mass fraction of the silk fibroin solution was adjusted to 1% with deionized water, and the adjusted silk fibroin solution was placed at -70°C for 0.5 hours, and then frozen at -5°C for 96 hours. After being put into a lyophilizer and dried for 48 hours, the nano silk protein porous material was obtained.

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Abstract

Relating to the technical field of biological materials, the invention provides a preparation method of a silk fibroin three-dimensional porous material. The method includes: firstly subjecting a silk fibroin solution to freezing treatment, then conducting refrigeration treatment on a frozen sample, taking the sample out, performing thawing under room temperature condition to obtain a state wet three-dimensional porous material with stable structure, and conducting vacuum drying on the material by freeze-drying method so as to obtain a dried silk fibroin porous material. The technology can control the pore size, secondary structure composition, porosity and the like of the nano-porous material by changing the silk fibroin water solution concentration, freezing and refrigeration temperature, treatment time and other parameters. The preparation process involved in the invention is mild, has no need of any cross-linking agent, pore-forming agent or other toxic organic reagents, has green and controllable preparation conditions, and no waste residue, is green and pollution-free, and is easy for industrialization.

Description

technical field [0001] The invention relates to a porous material and a preparation method thereof, in particular to a technology for preparing a three-dimensional porous material with a controllable secondary structure using silk fibroin as a raw material without the need for organic solvents or other additives. Background technique [0002] Defects and lesions of tissues and organs caused by various traumas and diseases are a major problem affecting human health. For tissue and organ defects, at present, organ transplantation is often used clinically for treatment. The effect of organ transplantation is relatively remarkable, but there are also big problems, and the shortage of donor sources is one of the more prominent points. The emergence of tissue engineering provides a more feasible method to solve this problem. [0003] Tissue engineering is to implant human living cells on degradable porous scaffold materials to regenerate living tissues or organs under the action...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/56A61L27/58
CPCA61L27/227A61L27/56A61L27/58A61L2400/12
Inventor 刘海峰姚丹语樊瑜波
Owner BEIHANG UNIV
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