Preparation method of porous three-dimensional silk fibroin material

A silk fibroin and porous material technology, applied in medical science, prosthesis, etc., can solve the problems of negative impact on biocompatibility, small pore size, slow degradation, etc., and achieve the effect of non-toxic side effects and good pore formation

Active Publication Date: 2012-06-27
SUZHOU UNIV
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Problems solved by technology

[0005] (1) In the Chinese invention patent "Preparation Method of Silk Fibroin Sponge Three-dimensional Porous Material" with publication number CN1262579C, methanol or ethanol needs to be used as a denaturant to promote the formation of silk fibroin II structure and improve the stability of silk fibroin in water. Also, because degradation is slow, uncontrollable, and the use of organic solvents may negatively affect biocompatibility
[0006] (2) In the Chinese invention patent "Silk Fibroin Porous Three-Dimensional Scaffold and Its Preparation Method" with the publication number CN101502669A, the three-dimensional scaffold is prepared by freeze-drying method, which avoids the use of organic solvents, but the porosity of the scaffold is not high, and there is still separation Sheet-like structure, and the nanocrystalline structure of the protein cannot be adjusted
[0007] (3) In the Chinese invention patent "Preparation method of a silk fibroin porous structure material" with the publication number CN1844509A, electrospinning is used to prepare a porous scaffold, but its pore size is small, and it is difficult to obtain a porous scaffold with a complex structure
[0008] At present, the salting-out method is still one of the most important methods for preparing porous silk protein scaffolds. However, the porous silk protein scaffolds prepared by the traditional salting-out method have high hardness, high crystallinity, and the surface structure of the porous scaffold wall cannot be controlled. problem, not suitable for soft tissue repair

Method used

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preparation example Construction

[0039] See figure 1 , figure 1 It is a flowchart of a method for preparing a silk fibroin porous three-dimensional material of the present invention. As shown in the figure, the preparation method includes steps:

[0040] S11: Prepare a silk fibroin solution, the silk fibroin solution has a first concentration;

[0041] S12: Concentrating the silk fibroin solution to make the silk fibroin solution have a second concentration, and the second concentration is greater than the first concentration;

[0042] S13: adjusting the mass fraction of the concentrated silk fibroin solution so that the mass fraction of the silk fibroin solution is 4% to 9%;

[0043] S14: Using a salting-out method, using sodium chloride as a pore-forming agent, the above silk fibroin solution is made into a scaffold and allowed to stand, and then the sodium chloride in the scaffold is removed to obtain a spongy tissue;

[0044] S15: After drying the spongy tissue, a dry silk fibroin porous material is ob...

Embodiment 1

[0052] Prepare silk fibroin solution. Specifically: put 50g silk into 0.5% Na 2 CO 3 Degumming was carried out in the solution and dried. During degumming, the silk was boiled at 100°C for 1 hour to remove sericin on the outside of the silk, rinsed with deionized water, and the above operation was repeated 3 times, and then the silk was dried at 60°C. Weigh 15 g of the degummed silk after the above treatment and put it into 100 mL of lithium bromide (LiBr) solution with a concentration of 9.3 mol / L for dissolving treatment, and dissolve at 60° C. for 4 hours during the dissolving treatment. Then above-mentioned LiBr solution that is dissolved with silk is carried out dialysis treatment, during dialysis, with the dialysis bag of molecular weight cut-off 3500, soak in deionized water and dialyze for 3 days, change water once every two hours during the period, to remove LiBr in the solution, thereby obtain pure Silk fibroin solution, its concentration is 6%.

[0053] The above...

Embodiment 2

[0058] A pure silk fibroin solution with a concentration of 6% was prepared by the same method as in Example 1.

[0059] The silk fibroin solution was concentrated at 80° C. for 8 hours, and the concentrated silk fibroin solution had a concentration of 20%. Then the massfraction of silk fibroin aqueous solution is adjusted to 4%;

[0060] The silk fibroin solution was salted out to make a scaffold, and after standing for 48 hours, it was soaked in water for 72 hours to remove NaCl and obtain a spongy tissue.

[0061] The nano silk protein porous material is obtained after natural drying at room temperature by adopting a slow drying treatment method.

[0062] See image 3 , image 3 It is the scanning electron microscope test of the above-mentioned silk fibroin scaffold in embodiment two. It was found that the pore size of the scaffold was about 319±15 μm, and the hardness was increased compared with the vacuum-dried scaffold with the same pretreatment.

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Abstract

The invention provides a preparation method of porous three-dimensional silk fibroin material, comprising the following steps of concentrating a silk fibroin solution, and manufacturing a bracket by the processed silk fibroin solution through a salting out method. By utilizing the self-assembling behaviors of silk fibroin, a secondary structure of the silk fibroin is improved. Compared with other methods of obtaining porous brackets, not only can the pore-forming property be good but also the inner walls of pores are in a very obvious fine structure.

Description

technical field [0001] The invention relates to a porous material and a preparation method thereof, in particular to a technique for preparing a three-dimensional porous material with a specific nanostructure and secondary structure by using silk fibroin as a raw material. Background technique [0002] Tissue engineering is the planting of human living cells on degradable porous scaffold materials to regenerate living tissues or organs under the action of growth factors to repair or replace the energy of damaged tissues or organs. The biomedical materials that have been developed and applied as tissue engineering scaffolds mainly include silicone rubber, polyurethane, polylactic acid (PLA), polyglycolic acid (PGA) and collagen. An ideal cell scaffold material should have good porosity, biocompatibility, and degradability, as well as certain mechanical properties. However, there are still many problems unsolved in biocompatibility, physical and chemical properties, degradati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/22
Inventor 吕强姚丹语赵荟菁刘珊珊
Owner SUZHOU UNIV
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