Preparation method of silk fibroin aerogel

A silk fibroin and aerogel technology, which is applied in the field of silk fibroin aerogel preparation, can solve the problems of difficult biodegradation and poor biocompatibility of aerogel materials, and achieves no toxic side effects and good energy storage capacity. , the effect of light weight

Active Publication Date: 2016-12-07
NANTONG TEXTILE & SILK IND TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] An object of the present invention is to solve the problems of existing airgel materials that are difficult to biodegrade and have poor biocompatibility, and to prepare a silk fibroin airgel material with better biocompatibility and easy degradation

Method used

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  • Preparation method of silk fibroin aerogel
  • Preparation method of silk fibroin aerogel
  • Preparation method of silk fibroin aerogel

Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Preparation of silk fibroin solution: take 80g silkworm cocoon shells with a balance, configure mass fraction as 0.01M, 4000 milliliters of sodium bicarbonate sodium carbonate aqueous solution with pH=9.5, add cocoon shells after heating to boiling with electromagnetic oven, adjust The power of the induction cooker is the lowest, continue to boil for 30 minutes, take out the cocoon silk and wash it with deionized water; repeat the above steps three times, remove the sericin, and then take it out and dry it in an oven at 60°C to obtain pure silk fibroin fiber. Dissolve pure silk fibroin fibers in a lithium bromide solution with a concentration of 9.3mol / L at a bath ratio of 20:150, and dissolve in a constant temperature magnetic stirrer at 60±2°C for about 1 hour. After cooling, take it out, put it into a dialysis bag and seal it, place it in deionized water for dialysis for 3-4 days, filter it with absorbent cotton to obtain a pure silk fibroin solution, calculate th...

Embodiment 2

[0033] (1) Preparation of silk fibroin solution: take by weighing 80g silkworm cocoon shells with precision balance, configuration mass fraction is 0.01M, 4000 milliliters of sodium bicarbonate sodium carbonate aqueous solution of pH=9.5, (add 4000 milliliters of deionized water in stainless steel, Sodium carbonate 1 gram, sodium bicarbonate 3 grams), add the cocoon shell after heating to boiling with the electromagnetic oven, adjust the electromagnetic oven power to be the lowest, continue to boil slightly for 30 minutes, take out the silk and clean it with deionized water; repeat the above steps three times to remove the silk Glue, and then take it out and place it in an oven at 60°C to dry to obtain pure silk fiber. Dissolve pure silk fiber in lithium bromide solution with a concentration of 9.3mol / L at a bath ratio of 20:150, dissolve it in a constant temperature magnetic stirrer at 60±2°C for about 1 hour, take it out after cooling, put it into a dialysis bag, seal it, and...

Embodiment 3

[0036](1) Preparation of silk fibroin solution: take by weighing 80g silkworm cocoon shells with precision balance, configuration mass fraction is 0.01M, 4000 milliliters of sodium bicarbonate sodium carbonate aqueous solution of pH=9.5, (add 4000 milliliters of deionized water in stainless steel, Sodium carbonate 1 gram, sodium bicarbonate 3 grams), add the cocoon shell after heating to boiling with the electromagnetic oven, adjust the electromagnetic oven power to be the lowest, continue to boil slightly for 30 minutes, take out the silk and clean it with deionized water; repeat the above steps three times to remove the silk Glue, and then take it out and place it in an oven at 60°C to dry to obtain pure silk fiber. Dissolve pure silk fiber in lithium bromide solution with a concentration of 9.3mol / L at a bath ratio of 20:150, dissolve it in a constant temperature magnetic stirrer at 60±2°C for about 1 hour, take it out after cooling, put it into a dialysis bag, seal it, and ...

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Abstract

The invention discloses a preparation method of a silk fibroin aerogel. The silk fibroin aerogel is prepared from domestic silkworm silks, and a silk fibroin water solution is obtained by conducting degumming, dissolving and dialyzing; and the silk fibroin aerogel is prepared by the following steps: 1) concentrating the silk fibroin water solution by conducting room-temperature air blasting or thinning the silk fibroin water solution with the addition of de-ionized water, so that the concentration of the silk fibroin water solution is regulated to be 15-60mg / ml; 2) keeping the silk fibroin water solution in a container and conducting high-speed shearing on the silk fibroin water solution by virtue of a stirrer for 10-60min, so that a great amount of bubbles are formed and the silk fibroin water solution is converted into bubble-shaped liquid; and 3) freeze-drying the bubble-shaped liquid in a die, so that the silk fibroin aerogel is obtained. The aerogel prepared by the invention is quite low in density, and the aerogel has the advantages of being light in mass, high in specific surface area, good in biocompatibility and the like; the aerogel is especially applicable to such aspects as body liquid adsorption, biological dressings, sustained release of drugs, tissue repair and the like; and the aerogel has a broad prospect when used as a bio-medical material.

Description

technical field [0001] The present invention relates to the field of airgel material preparation. More specifically, the present invention relates to a method for preparing silk fibroin airgel with a low-density porous structure. Background technique [0002] Airgel, also known as xerogel, is a highly dispersed solid material that is composed of colloidal particles or polymer molecules aggregated into a nanoporous network structure, and filled with gaseous media in the voids. It is a solid material with extremely low density, with a density of about 0.003-0.5g / cm 3 , the density can be as low as 0.001g / cm 3 . It is obviously different from porous materials with pore structures at the micron and millimeter scales, and has a very large specific surface area, with a specific surface area as high as 200-1000 m 2 / g, its void rate is as high as 80% to 99.8%, and its thermal conductivity is extremely low, about 0.015w / (m·k). The unique nanoporous structure makes it significan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61K47/42A61L27/22A61L15/42A61L15/32
CPCA61K47/42A61L15/32A61L15/425A61L26/0047A61L26/0085A61L27/227A61L27/56C08L89/00
Inventor 卢神州吴峰陈大旗付华卢欣旸
Owner NANTONG TEXTILE & SILK IND TECH RES INST
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