Preparation method of silk fibroin aerogel

Abstract

The invention discloses a preparation method of a silk fibroin aerogel. The silk fibroin aerogel is prepared from domestic silkworm silks, and a silk fibroin water solution is obtained by conducting degumming, dissolving and dialyzing; and the silk fibroin aerogel is prepared by the following steps: 1) concentrating the silk fibroin water solution by conducting room-temperature air blasting or thinning the silk fibroin water solution with the addition of de-ionized water, so that the concentration of the silk fibroin water solution is regulated to be 15-60mg / ml; 2) keeping the silk fibroin water solution in a container and conducting high-speed shearing on the silk fibroin water solution by virtue of a stirrer for 10-60min, so that a great amount of bubbles are formed and the silk fibroin water solution is converted into bubble-shaped liquid; and 3) freeze-drying the bubble-shaped liquid in a die, so that the silk fibroin aerogel is obtained. The aerogel prepared by the invention is quite low in density, and the aerogel has the advantages of being light in mass, high in specific surface area, good in biocompatibility and the like; the aerogel is especially applicable to such aspects as body liquid adsorption, biological dressings, sustained release of drugs, tissue repair and the like; and the aerogel has a broad prospect when used as a bio-medical material.

Description

technical field

[0001] The present invention relates to the field of airgel material preparation. More specifically, the present invention relates to a method for preparing silk fibroin airgel with a low-density porous structure. Background technique

[0002] Airgel, also known as xerogel, is a highly dispersed solid material that is composed of colloidal particles or polymer molecules aggregated into a nanoporous network structure, and filled with gaseous media in the voids. It is a solid material with extremely low density, with a density of about 0.003-0.5g / cm 3 , the density can be as low as 0.001g / cm 3 . It is obviously different from porous materials with pore structures at the micron and millimeter scales, and has a very large specific surface area, with a specific surface area as high as 200-1000 m 2 / g, its void rate is as high as 80% to 99.8%, and its thermal conductivity is extremely low, about 0.015w / (m·k). The unique nanoporous structure makes it significan...

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