Injection gel containing submicron hyaluronic acid microspheres and preparation method thereof

A hyaluronic acid and sub-micron-level technology, which is applied in the fields of repairing defective tissues or constructing organs, manufacturing medical materials, and tissue engineering technology preparation, can solve problems such as poor plasticity, achieve no inflammation, good biocompatibility, The effect of low heavy metal content

Inactive Publication Date: 2012-12-12
冯淑芹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its free flow behavior makes its plasticity relatively poor, thus limiting its application

Method used

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  • Injection gel containing submicron hyaluronic acid microspheres and preparation method thereof
  • Injection gel containing submicron hyaluronic acid microspheres and preparation method thereof
  • Injection gel containing submicron hyaluronic acid microspheres and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Preparation of nano / submicron microspheres

[0034] The purified hyaluronic acid with a weight fraction of 0.2% was dissolved in the organic solvent trifluoroethanol to obtain a transparent and uniform electrospinning dope.

[0035] Electrospinning was performed with the prepared electrospinning dope. The spinning parameters were controlled at a voltage of 15kv, a nozzle aperture of 0.4mm, a solution flow rate of 1ml / h, an ambient temperature of 20°C, and a spacing of 15cm between the receiving plate and the nozzle.

[0036] In a 500ml container with a dielectric function, add the prepared alkaline solution with pH>10 and a concentration of 0.05M, and add 1g / 500ml of adipic acid dihydrazide (ADH) at the same time, and the reaction temperature in the container is 5 ℃ , hyaluronic acid gel microspheres with cross-linking function were obtained after electrospinning.

[0037](2) soaking, washing, and extracting the nano-microspheres prepared in the step 1 with absolu...

Embodiment 2

[0041] (1) Preparation of nano / submicron microspheres

[0042] The purified hyaluronic acid was dissolved in trifluoroethanol and water with a volume ratio of 80 / 20 to prepare a weight fraction of 1% to obtain a transparent and uniform electrospinning dope.

[0043] Electrospinning was performed with the prepared electrospinning dope. The spinning parameters were controlled at a voltage of 5kv, a nozzle aperture of 0.2mm, a solution flow rate of 0.5ml / h, an ambient temperature of 20°C, and a spacing of 5cm between the receiving plate and the nozzle.

[0044] In a 500ml container with a dielectric function, add the prepared alkaline solution with pH>10 and a concentration of 4M, and at the same time add 25g / 500ml adipic acid dihydrazide (ADH), the reaction temperature in the container is 100 ℃, Hyaluronic acid gel microspheres with cross-linking function were obtained by electrospinning.

[0045] (2) soaking, washing, and extracting the submicron microspheres prepared in the ...

Embodiment 3

[0049] (1) Preparation of nano / submicron microspheres

[0050] The purified hyaluronic acid was dissolved in hexafluoroisopropanol and water solvent (volume ratio 50 / 50) to prepare a weight fraction of 5% to obtain a transparent and uniform electrospinning dope.

[0051] Electrospinning was performed with the prepared electrospinning dope. The spinning parameters were controlled at a voltage of 10kv, a nozzle aperture of 1mm, a solution flow rate of 0.2ml / h, an ambient temperature of 20°C, and a spacing of 5cm between the receiving plate and the nozzle.

[0052] In a container with a dielectric function, add the prepared alkaline solution with pH>10 and a concentration of 1M, and at the same time add 1g / ml adipic acid dihydrazide (ADH), the reaction temperature in the container is 50 ℃, the static electricity The hyaluronic acid gel microspheres with cross-linking function were obtained by spinning.

[0053] (2) soaking, washing, and extracting the nano-microspheres prepared...

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Abstract

The invention discloses an injection gel containing submicron hyaluronic acid microspheres and a preparation method thereof. The diameter of each microsphere is 100 nanometers to 50 micrometers. Swelling ratio of the injection gel is 2-2000. The preparation method includes: dissolving biodegradable hyaluronic acid into organic solvent to obtain spinning solution; electrospinning the spinning solution using electrostatic spinning, and crosslinking through coagulating bath to obtain nano / submicron microspheres; dialyzing to purify the microspheres, preparing into injection with phosphate buffer or normal saline, and homogenizing with suspension of a stem cell growth factor system according to a certain volume ratio to obtain the injection gel. The injection gel containing submicron hyaluronic acid microspheres prepared by the method is fine in shaping performance, long in shape preservation, and fine in cell compatibility, and is applicable as therapeutic for ophthalmology and orthopedics, also applicable as tissue-engineered repair materials such as bone tissues, cartilage tissues, blood vessel tissues, heart tissues and nerve tissues or as filling material for plastic surgery.

Description

technical field [0001] The invention relates to an injection gel containing submicron hyaluronic acid microspheres and a preparation method, in particular to a tissue engineering technology preparation method for repairing defective tissues or constructing organs, belonging to the technical field of medical material manufacturing methods. Background technique [0002] Injectable gel is a kind of smart polymer material, which is a relatively new research field developed in the past ten years and a very hot research topic of smart polymer materials. Injectable gel has good chimerality between the body and the repaired tissue, and achieves minimally invasive in situ injection, becoming a hot spot for soft tissue repair, reconstruction, and filling. But how to use the principle of biomimicry and use the organisms in nature as a blueprint to develop materials that are functionally close to or even exceed biological tissues, so as to realize the transition from biologically inacti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/52
Inventor 冯淑芹
Owner 冯淑芹
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