Silk fibroin porous three-dimensional material and preparation method thereof

A silk fibroin, three-dimensional technology, applied in medical science, prosthesis, etc., can solve the problem of small pore size

Active Publication Date: 2009-08-12
苏州盛泽科技创业园发展有限公司
View PDF3 Cites 33 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The porous material made of superimposed fibers obtained by electrospinning has a small pore size, which may be limited when used as a biomedical material

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Silk fibroin porous three-dimensional material and preparation method thereof
  • Silk fibroin porous three-dimensional material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation steps of the silk fibroin porous three-dimensional material provided in this embodiment are as follows:

[0023] 1. Put 1 kg of cocoon shells into 50 liters of neutral soap solution with a mass concentration of 0.5%, and treat them at 98-100°C for 2 hours to degumming the cocoon shells, and obtain pure silk fibroin (silk fibroin) after fully washing . The dried pure silk fibroin is dissolved in 5 liters of 9.3 mol / liter lithium bromide aqueous solution with stirring at 65±2° C. to form a silk fibroin mixed solution.

[0024] 2. Using a cellulose membrane as a dialysis material, dialyzing the obtained silk fibroin mixed solution with deionized water to remove impurities such as lithium bromide to obtain a pure silk fibroin solution.

[0025] 3. Adjust the mass concentration of the silk fibroin solution to 5%.

[0026] 4. Pour the silk fibroin solution into a stainless steel box, and quickly freeze at -30°C for 5 hours to obtain a silk fibroin solution f...

Embodiment 2

[0033] Put 1 kg of raw silk into 30 liters of sodium carbonate aqueous solution with a mass concentration of 0.2%, treat it at 98-100°C for 2 hours, degumming the raw silk, and obtain pure silk fibroin (ie silk fibroin) after fully washing. The dried pure silk fibroin is heated and dissolved at 75±5°C with 8 liters of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 to obtain a silk fibroin mixed solution.

[0034] The cellulose membrane is used as a dialysis material, and the obtained silk fibroin mixed solution is dialyzed with deionized water to remove impurities such as calcium chloride to obtain a pure silk fibroin solution.

[0035] Adjust the mass concentration of silk fibroin solution to 2%.

[0036] The silk fibroin solution was poured into an aluminum box, and quickly frozen at -60°C for 20 hours to obtain a silk fibroin solution frozen body.

[0037] Store the frozen body in a refrigerator at -10°C, and freeze and crystallize for 60 days.

[...

Embodiment 3

[0041] Put 100 grams of waste silk into 4 liters of sodium carbonate aqueous solution with a mass concentration of 0.05%, degumming at 98-100° C. for 0.5 hours, repeat the treatment 3 times, and obtain pure silk fibroin (ie silk fibroin) after sufficient washing. The dried pure silk fibroin is dissolved in 50 ml of 9.3 mol / L lithium bromide aqueous solution with stirring at 65±2° C. to form a mixed silk fibroin solution.

[0042] The cellulose membrane is used as a dialysis material, and the obtained silk fibroin mixed solution is dialyzed with deionized water for 3 days to obtain a pure silk fibroin solution.

[0043] Concentrate with 10% polyethylene glycol solution to obtain a silk fibroin solution with a mass concentration of 15%.

[0044] The silk fibroin solution was poured into a stainless steel box, and quickly frozen at -20°C for 10 hours to obtain a silk fibroin solution frozen body.

[0045] Store the frozen body in a refrigerator at -5°C, and freeze and crystalliz...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to a porous material and a preparation method thereof, in particular to a technology in which silk fibroin is taken as a raw material to prepare porous three-dimensional materials. In the method, silk fibroin solution is injected into a metal mould, 1-24 hours of quick freezing is carried out on the solution at the low temperature of minus 10-minus 80 DEG C, thus obtaining afrozen body; the frozen body is preserved at the temperature of minus 5-minus 25 DEG C for 2-60 days, thus obtaining frozen crystalline solid; then the frozen crystalline solid is thawed and dried, thus obtaining the silk fibroin porous three-dimensional material. The silk fibroin of the porous material is fibroin I type crystal structure, therefore, the silk fibroin is insoluble in water and canbe completely degraded in a while and is finally degraded into nonpoisonous amino acid; the material of the invention has mutual run-through pores with aperture of 10-500 microns; the pores and pore diameter are relatively large, thus being suitable for growth of cells; meanwhile, in the process of preparation, no chemical cross linking agent, foaming agent or denaturing agent are needed, thus retaining fine biocompatibility of the silk fibroin.

Description

technical field [0001] The invention relates to a porous material and a preparation method thereof, in particular to a technique for preparing a porous three-dimensional material using silk fibroin as a raw material. The prepared material can be applied to technical fields such as biomedicine, biotechnology, and tissue engineering. Background technique [0002] Biomedical materials, especially tissue engineering scaffold materials, tissue repair regeneration materials, etc., require that the used biomaterials have good biocompatibility, and must also have certain bioactivity, biodegradability and three-dimensional porous structure. [0003] Silk is composed of about 75% silk fibroin and about 25% sericin, and is a fairly pure natural protein fiber. Studies have shown that silk fibroin is non-toxic, non-irritating, has good biocompatibility, and has certain biodegradability. In recent years, comparative studies on various biological materials have shown that silk fibroin has...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/56A61L27/58
Inventor 卢神州李明忠
Owner 苏州盛泽科技创业园发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap