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Water-insoluble silk fibroin porous material and preparation method thereof

A silk fibroin and porous material technology, which is applied in the field of preparing water-insoluble porous materials, can solve problems such as reducing water solubility and affecting the biocompatibility of silk fibroin materials, and achieve the effect of meeting functional requirements

Active Publication Date: 2010-10-13
SUZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alcohol denaturation method to prepare porous silk fibroin materials, organic solvents may affect the biocompatibility of silk fibroin materials, therefore, there may be certain restrictions when used as biomedical materials
In the Chinese invention patent "Preparation method of a silk fibroin porous structure material" with the publication number CN1844509A, a kind of silk fibroin porous fiber material is obtained by electrospinning. The material still has certain water solubility and requires Treatment with toxic organic solvent methanol to make it insoluble may affect the biocompatibility of silk fibroin materials
The Chinese invention patent with the publication number CN1118518C discloses a porous silk fibroin film and its preparation method. It adopts a freeze-drying method. The structure of its silk fibroin protein is mainly non-crystalline structure. In order to reduce the water solubility of the material, the method A chemical cross-linking agent was added to the silk fibroin solution, which may affect the biocompatibility of the silk fibroin material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation steps of the silk fibroin porous three-dimensional material provided in this embodiment are as follows:

[0022] 1. Put 0.5 kg of cocoon shells into 25 liters of neutral soap solution with a mass concentration of 0.5%, and treat them for 2 hours at a temperature of 98-100° C. to degumming the cocoon shells, and obtain pure silk fibroin ( the silk fibroin). Dissolve the dried pure silk fibroin into a mixed solution of silk fibroin with 2.5 liters of 9.2 mol / liter lithium bromide aqueous solution and stir at a temperature of 60±2°C;

[0023] 2. Using cellulose membrane as the dialysis material, dialyze the obtained silk fibroin mixed solution with deionized water to remove impurities such as lithium bromide to obtain pure silk fibroin solution;

[0024] 3. Add salt solution to the silk fibroin solution, adjust the mass concentration of silk fibroin in the silk fibroin solution to be 3%, and make the concentration of sodium chloride in the final silk fibro...

Embodiment 2

[0030] Put 0.1 kg of raw silk into 3 liters of 0.2% sodium carbonate aqueous solution, and treat it at 98-100°C for 2 hours to degumming the raw silk, and get pure silk fibroin (ie silk fibroin) after fully washing. The dried pure silk fibroin is heated and dissolved in 1 liter of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 at 75±5° C. to obtain a silk fibroin mixed solution.

[0031] The cellulose membrane is used as a dialysis material, and the obtained silk fibroin mixed solution is dialyzed with deionized water to remove impurities such as calcium chloride to obtain a pure silk fibroin solution.

[0032] Add salt solution in silk fibroin solution, adjust to the mass concentration of silk fibroin in the final silk fibroin solution be 2%, potassium chloride concentration reaches 1 gram / liter, zinc chloride concentration reaches 0.001 gram / liter, calcium nitrate concentration Reached 0.002 g / L.

[0033] The silk fibroin solution was injected into...

Embodiment 3

[0038] Put 200 grams of waste silk into 8 liters of sodium carbonate aqueous solution with a mass concentration of 0.05%, degumming at 98-100° C. for 0.5 hours, repeat the treatment 3 times, and obtain pure silk fibroin (ie silk fibroin) after sufficient washing. The dried pure silk fibroin is dissolved in 500 ml of 9 mol / liter lithium bromide aqueous solution with stirring at 65±2° C. to form a mixed silk fibroin solution.

[0039] The cellulose membrane is used as a dialysis material, and the obtained silk fibroin mixed solution is dialyzed with deionized water for 3 days to obtain a pure silk fibroin solution.

[0040]Concentrate with 10% polyethylene glycol solution to obtain silk fibroin solution, add salt solution, adjust the mass concentration of silk fibroin in the final silk fibroin solution to be 10%, the concentration of sodium sulfate reaches 2 grams per liter, and the concentration of calcium nitrate reaches 0.003 g / L, the concentration of zinc chloride reaches 0....

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Abstract

The invention discloses a water-insoluble silk fibroin porous material and a preparation method thereof. The preparation method comprises the following steps of: adding one or more kinds of salt solution into silk fibroin solution, mixing the solution uniformly and then injecting the mixture into a metal die, freezing the mixture for 24 hours at the temperature of between 10 DEC below zero and 80 DEG C below zero, performing freeze drying on the mixture, and treating the mixture at the temperature of between 4 and 120 DEG C under the environment with the humidity of 30 to 98 percent to form the water-insoluble silk fibroin porous material. The solution loss rate of the water-insoluble silk fibroin porous material in 37 DEG C water for 24 hours is below 2 percent, and the material can be fully degraded into no-toxicity amino acid; the material has large holes and apertures, and is suitable for the growth of cells; and meanwhile, any chemical cross-linking agent, foaming agent or denaturing agent and the like does not need to be added during preparation, so good biocompatibility of the silk fibroin is kept, and the silk fibroin can be used for wound recovery materials for cell culture, skin, bones and gristles and medicinal release-controlled materials for temporary coverage, tissue isolation and hemostasis and the like.

Description

technical field [0001] The invention relates to a porous material and a preparation method thereof, in particular to a technology for preparing a water-insoluble porous material using silk fibroin as a raw material. The prepared material can be applied to technical fields such as biomedicine, biotechnology, and tissue engineering. Background technique [0002] Biomaterials used in tissue engineering scaffolds should have good biocompatibility, certain bioactivity, biodegradability and three-dimensional porous structure. In addition, the important condition that should be met is that it should remain stable for a certain period of time in a physiological environment, and it is not easy to be dissolved by body fluids, otherwise it will be difficult to play the role of a scaffold. Biomaterials used for temporary wound coverage, tissue isolation, hemostasis, drug controlled release, etc., as well as biomaterials used as biosensors, cell culture carriers, etc., also require insol...

Claims

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Application Information

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IPC IPC(8): C08L89/00C08K3/16C08K3/30C08K3/28C08J9/28
Inventor 卢神州李明忠吴徵宇
Owner SUZHOU UNIV
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