Water-insoluble silk fibroin porous material and preparation method thereof

Abstract

The invention discloses a water-insoluble silk fibroin porous material and a preparation method thereof. The preparation method comprises the following steps of: adding one or more kinds of salt solution into silk fibroin solution, mixing the solution uniformly and then injecting the mixture into a metal die, freezing the mixture for 24 hours at the temperature of between 10 DEC below zero and 80 DEG C below zero, performing freeze drying on the mixture, and treating the mixture at the temperature of between 4 and 120 DEG C under the environment with the humidity of 30 to 98 percent to form the water-insoluble silk fibroin porous material. The solution loss rate of the water-insoluble silk fibroin porous material in 37 DEG C water for 24 hours is below 2 percent, and the material can be fully degraded into no-toxicity amino acid; the material has large holes and apertures, and is suitable for the growth of cells; and meanwhile, any chemical cross-linking agent, foaming agent or denaturing agent and the like does not need to be added during preparation, so good biocompatibility of the silk fibroin is kept, and the silk fibroin can be used for wound recovery materials for cell culture, skin, bones and gristles and medicinal release-controlled materials for temporary coverage, tissue isolation and hemostasis and the like.

Description

technical field

[0001] The invention relates to a porous material and a preparation method thereof, in particular to a technology for preparing a water-insoluble porous material using silk fibroin as a raw material. The prepared material can be applied to technical fields such as biomedicine, biotechnology, and tissue engineering. Background technique

[0002] Biomaterials used in tissue engineering scaffolds should have good biocompatibility, certain bioactivity, biodegradability and three-dimensional porous structure. In addition, the important condition that should be met is that it should remain stable for a certain period of time in a physiological environment, and it is not easy to be dissolved by body fluids, otherwise it will be difficult to play the role of a scaffold. Biomaterials used for temporary wound coverage, tissue isolation, hemostasis, drug controlled release, etc., as well as biomaterials used as biosensors, cell culture carriers, etc., also require insol...