29 results about "Cellular infiltration" patented technology

The application discloses application of DUSP4 in preparation of a marker for predicting the curative effect of immunotherapy of hepatocellular carcinoma and a sensitizing drug, and belongs to the technical field of biological medicine.The application finds that high expression of dual specificity phosphatase 4 (DUSP4) is significantly related to high response rate and long survival period of an immunological checkpoint blocking therapy for a hepatocellular carcinoma patient.DUSP4 promotes CD8+ T cell and NK cell infiltration and remodels an immune microenvironment by inhibiting a TGF-beta signal path, down-regulating an immunosuppressive factor and up-regulating an antigen presenting molecule and a chemotactic factor.The application provides a kit and a method for detecting the expression level of DUSP4 to predict an immunotherapy response, and a combined drug composition comprising a DUSP4 activator or a TGF-beta inhibitor and an immunological checkpoint inhibitor.Experiments prove that up-regulation of the expression of DUSP4 can significantly enhance T cell killing function, and produces a synergistic anti-tumor effect with an anti-PD-1 antibody.The application provides a new strategy for precise stratified treatment and overcoming immunological drug resistance of hepatocellular carcinoma.

This invention relates to the field of traditional Chinese medicine and natural medicines, and discloses a traditional Chinese medicine composition for treating thyroiditis, nodular goiter, and thyroid nodules, as well as its preparation method and application. The traditional Chinese medicine composition is made from raw materials in parts by weight of 1-30 parts of Prunella vulgaris, 1-30 parts of Taraxacum mongolicum, 1-30 parts of Hordeum vulgare malt, 1-20 parts of Citrus reticulata peel, 1-24 parts of Crataegus pinnatifida, and 1-24 parts of Raphanus sativus seed. It can not only significantly reduce serum TgAb and TPOAb antibody levels, inhibiting the attack of autoimmune response on the thyroid gland from the source, but also effectively reduce lymphocyte infiltration, repair damaged thyroid follicular structure and restore colloid content, thereby reversing immune damage and tissue destruction of the thyroid gland, reducing thyroid nodules, and solving the problems of existing treatments for thyroiditis, nodular goiter, and thyroid nodules that cannot reverse immune damage to the thyroid gland, destruction of follicular structure, and tissue fibrosis.

The application belongs to the technical field of biological medicine, and relates to application of a CXCL14 inhibitor in preparation of a drug for preventing or treating abdominal aortic aneurysm of a patient. The inhibitor effectively eliminates the gender difference of abdominal aortic aneurysm by specifically blocking the combination of CXCL14 and its receptor CXCR4, significantly reduces aortic macrophage infiltration and PDGFRA + macrophage accumulation in the progress of abdominal aortic aneurysm, reduces the degree of collagen degradation and elastic fiber damage, and thus inhibits the pathological progress of abdominal aortic aneurysm. The application provides a new target and drug combination for precise treatment of male abdominal aortic aneurysm, and has clear clinical application value.

This invention provides the application of type IV collagen and its encoding gene in the prognostic assessment and treatment of nasopharyngeal carcinoma (NPC). Research has shown that high expression of type IV collagen and its encoding gene COL4A1 is significantly associated with lower T-cell infiltration in patients with recurrent NPC; compared to NPC patients with low expression of type IV collagen, those with high expression have worse LRRFS and OS; and the expression level of the type IV collagen encoding gene COL4A1 is significantly associated with worse PFS in NPC patients. Therefore, type IV collagen and its encoding gene COL4A1 can serve as specific prognostic biomarkers for assessing the prognosis of NPC patients. Furthermore, inhibiting the expression of type IV collagen can effectively inhibit the growth of NPC; therefore, agents that inhibit type IV collagen expression can be used as drugs to inhibit immune escape in NPC and for the treatment of NPC.

The application discloses application of a PRMT3 inhibitor SGC707 in preparation of a drug for treating pulmonary fibrosis, and for the first time verifies that a selective PRMT3 inhibitor SGC707 can dose-dependently inhibit expression of fibrosis markers such as COL1A1 and alpha-SMA in fibroblasts induced by TGF-beta 1 in vitro; in a bleomycin (BLM) induced mouse pulmonary fibrosis model, SGC707 administration can significantly improve lung tissue collagen deposition, reduce inflammatory cell infiltration, repair alveolar structure damage, and effectively down-regulate expression levels of fibrosis related proteins in lung tissues. It is proved that PRMT3 is a functional target with important intervention value in pulmonary fibrosis, SGC707 has definite anti-pulmonary fibrosis activity in vitro and in vivo, and a new drug strategy is provided for treatment of pulmonary fibrosis.

This invention belongs to the field of biomedical technology and relates to the application of OSM inhibitors in the preparation of drugs for the prevention or treatment of calcific aortic valve disease. This invention reveals for the first time that OSM drives osteogenic differentiation and calcified nodule formation of valvular interstitial cells through the OSM-OSMR-JAK2 / JAK3-STAT3-RUNX2 signaling axis, which is the core pathogenic mechanism leading to calcific aortic valve disease. This invention utilizes anti-OSM neutralizing antibodies or sulforaphane to effectively block this signaling axis, significantly inhibiting macrophage infiltration, valvular calcification, and improving hemodynamic abnormalities. This invention provides a precise target and a novel strategy for the modification therapy of calcific aortic valve disease, solving the technical problem of the ineffectiveness of traditional broad-spectrum anti-inflammatory therapy in valvular calcification.

The present application relates to a new use of halogenophenol compound 4,5,2'-trihydroxy-2,5'-dibromobenzophenone, in particular in the preparation of a drug for treating inflammatory bowel disease. The halogenophenol compound of the present application can significantly alleviate the weight loss and colon shortening of the colonitis model, reduce the disease activity index of the colonitis model, reduce the inflammatory cell infiltration of the colonitis model, reduce the expression of inflammatory factors, has an immune regulation function on the intestinal tract, and is superior to the clinical first-line drug mesalazine in most indicators, and has application prospects in the treatment of inflammatory bowel disease.

The present application relates to the technical field of protein genomics analysis, and particularly relates to a protein gene multiomics analysis method, which comprises: S1. sample collection and pretreatment: tumor tissue and paired normal adjacent tissue are selected as samples, and the samples are frozen and crushed; S2. sample multiomics detection: the frozen and crushed samples are subjected to genomics detection and proteomics detection; S3. sample multiomics data processing and analysis: the genomics detection result and the proteomics detection result of the samples are obtained, and tumor molecular characteristics are identified through multiomics data integration analysis.The present application carries out systematic protein genomics analysis on LCNEC tumor tissue and NATs. The research not only reveals the genomic abnormality characteristics of LCNE and disease-related molecular phenotypes, but also deepens the scientific cognition of patient stratification logic in targeted therapy strategies. Further research finds that IL33, as a new key therapeutic biomarker, is related to T cell infiltration and has anti-tumor activity.

The application relates to an endometriosis subtype identification method and system, relates to the fields of bioinformatics and medical diagnosis auxiliary technologies, and takes transcriptome expression data as input, constructs a standardized expression matrix, and adopts consistency clustering to perform subtype modeling to obtain a molecular subtype label; expression timing structure of a menstrual cycle phase is introduced to perform dynamic gene identification and discriminant power evaluation, and immune cell infiltration inference and path activity scoring are combined to analyze differences of the subtypes in an immune and inflammation network, then gene-pathway / gene-cell correlation is calculated to form multi-dimensional typing information with mechanism orientation. Based on the above results, an interpretable typing identification model is constructed, which provides a new technical means for accurate diagnosis and target identification of endometriosis.

This application provides a hUC-MSC cell suspension formulation for treating allergic rhinitis and its preparation method. It comprises mammalian stem cells, a pharmaceutically acceptable suspension carrier, and a protective agent. The mammalian stem cells are mesenchymal stem cells, which can be derived from the human umbilical cord. The formulation is suitable for intravenous administration. The preparation method includes: washing the resuscitated stem cells with phosphate buffer, centrifuging, resuspending them in a suspension carrier, adding the suspension carrier and protective agent, and mixing thoroughly. Experiments show that this formulation can reduce the levels of interleukin-4 and interleukin-5 in the serum and spleen of mice with allergic rhinitis, and alleviate inflammatory cell infiltration in the nasal mucosa.

The application discloses application of chrysin in preparation of a medicine for treating primary Sjogren's syndrome and belongs to the technical field of biological medicine. The application proves that chrysin can be specifically combined with TNFR2 protein and is a new type of TNFR2 inhibitor through molecular docking, molecular dynamics simulation, CETSA experiment and SPR experiment. Animal experiments show that chrysin can significantly increase the secretion amount of saliva and tear of a mouse model of primary Sjogren's syndrome, reduce the serum SSA and SSB antibody levels, reduce lymphocyte infiltration and acinus atrophy of submandibular glands and lacrimal glands, inhibit the expression of a cytokine INF-gamma, and the drug efficacy is equivalent to that of a positive medicine hydroxychloroquine; chrysin and gaoshunyujin have a synergistic effect, and the improvement effect on primary Sjogren's syndrome is better than that of hydroxychloroquine. The application provides a new medical use of chrysin, and provides a safe and effective prevention and treatment medicine for primary Sjogren's syndrome.

The application provides application of a CSF1R inhibitor combined with microwave ablation in treatment of lung cancer tissue, the combined treatment of microwave ablation and the CSF1R inhibitor significantly enhances T cell infiltration, after the combined treatment, the proportion of total immune cells is significantly increased, the proportion of macrophages in immune cells, the proportion of M2 type macrophages in macrophages and the proportion of M2 type macrophages and M1 type macrophages in the combined treatment group of microwave ablation and the CSF1R inhibitor are significantly reduced, and the proportion of CD4+ T cells and CD8+ T cells in T cells is significantly enhanced; the anti-tumor effect of the combined treatment of microwave ablation and the CSF1R inhibitor is significantly higher than that of the single treatment group, and the safety is good.

The application discloses a multifunctional piezoelectric and immune-regulating PG@ZnO composite fiber membrane, a preparation method and application thereof, and belongs to the field of biological medicines.The PG@ZnO composite fiber membrane comprises a PG fiber membrane composed of left-handed polylactic acid (PLLA) and gelatin and ZnO nanoparticles distributed on the surface and inside the fibers of the PG fiber membrane.Based on the piezoelectric characteristics of PLLA and the antibacterial and immune-regulating functions of the ZnO nanoparticles, the application constructs a PG@ZnO composite fiber membrane which has mechanical support, piezoelectric response and immune microenvironment regulation functions.The composite fiber membrane is used in pelvic floor repair, has good biocompatibility, can effectively promote HUVECs to form tubes and can induce macrophages to polarize to M2 type.In a rat abdominal wall muscle defect model, the PG@ZnO composite fiber membrane significantly inhibits inflammatory cell infiltration, promotes collagen ordered deposition and angiogenesis and accelerates functional regeneration of tissues.

The invention discloses application of oroxylum indicum total flavonoids in preparation of a medicine for treating uveitis. Experiments prove that the eye drops taking the oroxylum indicum total flavonoids or the oroxylin A as the active ingredient can reduce the uveitis eye inflammation and retina injury degree and reduce the infiltration quantity of inflammatory cells. The oroxylum indicum general flavone is taken as an oral medicine, so that inflammatory infiltration cells of a vitreous cavity and an iris-ciliary body can be reduced, bleeding symptoms can be effectively improved, hyperplasia of iris-ciliary body tissues can be inhibited, and infiltration of leukocytes of aqueous humor in mouse eyes can be effectively inhibited.

This invention provides the use of a reagent overexpressing Parp7 in the preparation of drugs for treating septic cardiomyopathy, belonging to the field of biomedical technology. This invention involves constructing a recombinant adeno-associated virus vector AAV9-F4 / 80- that specifically overexpresses PARP7 on macrophages. Parp7 The drug was intravenously injected into mice, and a septic cardiomyopathy model was established using lipopolysaccharide induction. Experimental results showed that macrophage-specific overexpression of PARP7 significantly reduced serum levels of myocardial injury markers CK-MB and cTnT, improved cardiac function indicators such as ejection fraction and fractional shortening, alleviated myocardial tissue pathological damage, and inhibited macrophage infiltration and inflammatory factors. Ifn-beta , Tnf- α , Il‑6 and Il‑1β The expression of PARP7. This invention reveals the protective role of PARP7 in septic cardiomyopathy, providing a new target and strategy for the treatment of septic cardiomyopathy.

This invention discloses the application of LukS-PV and its combination with a PD-1 inhibitor in the preparation of drugs for treating hepatocellular carcinoma, belonging to the field of biomedical technology. LukS-PV of this invention exerts its anti-tumor effect by promoting the polarization transformation of tumor-associated macrophages from M2 to M1 types, thereby improving the tumor immunosuppressive microenvironment. Furthermore, the combined use of LukS-PV and a PD-1 inhibitor can enhance CD8+ T cell infiltration and anti-tumor immune function, alleviate T cell exhaustion, and improve the therapeutic effect of liver cancer.

This invention provides the application of the LHPP gene in liver cancer immunotherapy and liver cancer immunotherapy drugs. Reagents that promote LHPP gene expression can be used to prepare liver cancer immunotherapy drugs. This invention also provides an mRNA nanomedicine, which is prepared by the following method: [The invention then describes a process involving Meo-PEG-Dlink]. m A PLGA solution, an aqueous solution of LHPP gene mRNA, and a cationic liposome solution were mixed and added dropwise to water under stirring to obtain a nanosolution. The nanosolution was then transferred to an ultrafiltration membrane and centrifuged. This invention is the first to discover that LHPP can participate in the immunotherapy of liver cancer and is associated with the survival prognosis of liver cancer. LHPP enhances STAT1 nuclear translocation by affecting the expression of autophagy-regulated IFNβ, thereby promoting the expression of T cell-related chemokines secreted by liver cancer cells, and ultimately promoting CD8+ expression. + T cell infiltration. Therefore, the LHPP gene can serve as a novel target for immunotherapy of liver cancer. The mRNA nanomedicine described has advantages such as long circulation time in the blood, high accumulation in tumors, rapid mRNA release, and low cost.

The application relates to the technical field of biological medicine, and particularly discloses a telomere-related gene marker for hepatocellular carcinoma prognosis prediction, a prognosis risk scoring model and application thereof. The application integrates the expression profile of a telomere-related gene in a TCGA-LIHC queue and clinical data, uses consistent clustering analysis to identify two telomere subtypes with significant survival difference, and further uses LASSO-Cox regression to construct a hepatocellular carcinoma prognosis risk scoring model containing 20 characteristic genes. The model shows excellent prediction performance in an internal training set and an external validation set, and can accurately distinguish high-risk and low-risk patients. The risk score is closely related to a tumor immune microenvironment, and high-risk patients show an immune inhibition state, such as reduced CD8+ T cell infiltration, which provides a basis for predicting the response of patients to an immune checkpoint inhibitor.

This invention belongs to the field of biomedical technology, specifically disclosing an injectable embolic agent, its preparation method, and its application. The embolic agent is a hydrocolloid system containing core-shell structured nanoparticles: the core contains Cu... 2+ Salt and ATOX1 inhibitors, where ATOX1 inhibitors can inhibit copper ion efflux and promote intracellular copper accumulation, and interact with Cu 2+ It synergistically induces copper death in tumor cells; the shell is a thermosensitive block copolymer hydrogel that can interact with Cu. 2+ Coordination bonds are formed, enhancing the stability of nanoparticles, while a sol-gel transition occurs at physiological temperatures, enabling precise vascular embolization; the overall core-shell structure allows for the formation of Cu... 2+ The sustained-release effect of ATOX1 inhibitors was observed. In the VX2 rabbit hepatocellular carcinoma model, this embolization agent significantly improved tumor necrosis rate and reduced metastasis rate compared to traditional iodized oil embolization; it also downregulated hypoxia- and angiogenesis-related factors such as HIF-1α, VEGF, and CD31, inhibited MMP9-mediated tumor invasion and metastasis, and promoted CD8+. + T-cell infiltration effectively improves the tumor immunosuppressive microenvironment after transarterial chemoembolization.

The application discloses a targeted cytokine composition for immunotherapy and a preparation method thereof. The fusion protein comprises, from N-terminal to C-terminal, an anti-PD-L1 single-domain antibody, a hinge region, a double-mutated human IL-15, a hinge region, an anti-NKG2D single-domain antibody and a double-mutated human IgG1 Fc fragment. In vitro experiments show that the fusion protein can simultaneously bind to double targets, significantly promotes NKG2D-positive CD8-positive T cell proliferation and has weak effect on regulatory T cells, and presents synergistic effect in a tumor-immune cell co-culture system. In vivo pharmacodynamic research shows that in a humanized mouse model, the fusion protein significantly inhibits the growth of colon cancer, the number of CD8-positive T cell and NK cell infiltrations in the tumor is obviously increased, and the effect is better than that of non-targeting and single-targeting controls. The fusion protein provided by the application has long-acting circulation, tumor targeting enrichment and immune cell subpopulation precise regulation functions, and can be used for preparing an antitumor drug.

This application discloses a fiber scaffold and its preparation method, a fiber scaffold system and its preparation method, belonging to the field of medical devices. The scaffold has a hollow tubular structure, including an inner fiber layer, a fiber support structure, and an outer fiber layer. The fiber support structure is coaxially sleeved on the outer surface of the inner fiber layer, and the outer fiber layer covers the outer surfaces of the inner fiber layer and the fiber support structure. The fiber porosity of the inner fiber layer is less than or equal to that of the outer fiber layer. The fiber support structure is composed of electrospun fibers and includes annular bodies distributed along the axial direction of the scaffold, with axial spacing between adjacent annular bodies to provide radial support. This application utilizes the annular bodies to provide radial support, while the axial spacing provides deformation space for bending, giving the scaffold both radial stiffness and axial flexibility; the differentiated porosity design is beneficial for cell infiltration and tissue regeneration, making it suitable for vascular interventional therapy.

This invention belongs to the field of traditional Chinese medicine preparation technology, and provides a traditional Chinese medicine composition with anti-inflammatory, swelling-reducing, analgesic, and joint-relieving properties, its preparation method, and its application in the preparation of drugs for treating arthralgia. The method includes the following steps: Smilax glabra, Corydalis yanhusuo (processed with vinegar), and Stephania tetrandra are pulverized, mixed, decocted with water, filtered, the filtrate is concentrated, ethanol is added to precipitate, the supernatant is collected, the ethanol is recovered and concentrated to obtain a water-extracted alcohol-immersed extract, which is then mixed with maltodextrin, wet-granulated, and dried to obtain the traditional Chinese medicine composition. The traditional Chinese medicine composition obtained by this invention is based on the principles of promoting joint mobility, reducing swelling and relieving pain. It can significantly inhibit ankle joint swelling in rats with gouty arthritis, improve local inflammatory cell infiltration, and reduce synovial tissue hyperplasia in rats with gouty arthritis.

The application discloses application of piRNA-AMIPIR in preparation of a drug for improving heart injury after acute myocardial infarction. The application first discovers that piRNA-AMIPIR is highly expressed in myocardial tissue after acute myocardial infarction (MI), and the expression level is positively correlated with the myocardial injury degree. After administration of the antisense nucleotide Antagomir-AMIPIR designed based on the piRNA-AMIPIR in a mouse acute myocardial infarction model, the myocardial cell apoptosis is reduced, the active oxygen (ROS) accumulation is reduced, the inflammatory cell infiltration is inhibited, the heart contraction function is significantly improved, and the ischemia-induced heart injury is reduced. The application confirms the potential value of the piRNA-AMIPIR as a treatment target for heart injury after acute myocardial infarction, the antisense nucleotide provides a new candidate drug and treatment strategy for clinical treatment of acute myocardial infarction, and has important scientific research significance and clinical application prospect.

This invention belongs to the field of novel pharmaceutical uses, and more specifically, relates to the application of cinnabar extract in the preparation of drugs for the prevention and treatment of gastric ulcers. The preparation method of the extract is as follows: Cinnabar is extracted by reflux with a 60%–80% (v / v) ethanol solution. The extracts are combined, evaporated to dryness, and then mixed with water to obtain an extract. The extract is then diluted, adsorbed onto a macroporous resin, and eluted with a 35%–45% (v / v) ethanol solution. The eluent is then evaporated to dryness to obtain the final extract. Experiments show that this extract can significantly reduce gastric mucosal damage in mice, decrease the ulcer index, and increase the ulcer inhibition rate, with effects superior to the positive control drug ranitidine. Its mechanism of action includes reducing inflammatory cell infiltration, decreasing the content of pro-inflammatory factors, pepsin activity, and malondialdehyde content, and increasing antioxidant enzyme activity. This invention provides a new natural drug source for the treatment of gastric ulcers, with a clearly defined active pharmaceutical ingredient and promising clinical application prospects.

This invention relates to extracellular vesicles derived from human mesenchymal stem cells and their use. The extracellular vesicles derived from mesenchymal stem cells according to the present invention inhibit the expression of fibrosis-related proteins, wound closure, and cell infiltration, which are increased in lung epithelial cells due to TGF-β1 treatment. In addition, microRNAs contained in large quantities in the extracellular vesicles inhibit the gene expression of fibrosis-related proteins in lung epithelial cells and lung fibroblasts. These extracellular vesicles significantly reduce the expression of fibrosis-related proteins, collagen production, and fibrotic areas in a mouse model of pulmonary fibrosis. Therefore, the extracellular vesicles according to the present invention can be used to prevent and treat pulmonary fibrosis.

A traditional Chinese medicine composition for improving or treating acute lung injury and an application thereof. The traditional Chinese medicine composition is made of the following traditional Chinese medicine raw materials: Areca catechu 12-24 parts, Magnolia officinalis 6-12 parts, Amomum tsao-ko 3-6 parts, Anemarrhena asphodeloides 6-12 parts, Paeonia lactiflora 6-12 parts, Scutellaria baicalensis 6-12 parts, Glycyrrhiza uralensis 3-6 parts, Rheum officinale 6-12 parts, Forsythia suspensa 8-16 parts, Mentha haplocalyx 3-6 parts, Gardenia jasminoides 3-6 parts, Mirabilite 3-6 parts, and Bambusa 5-10 parts. In a mouse animal model experiment, the traditional Chinese medicine composition described herein not only can alleviate the phenomenon of inflammatory cell infiltration in the bronchoalveolar lavage fluid caused by LPS, reduce the inflammatory response, but also can help to improve the lung tissue pathological injury induced by LPS, maintain the alveolar structure integrity, inhibit pulmonary edema, down-regulate the level of inflammatory factors in the bronchoalveolar lavage fluid, and inhibit the expression of inflammatory factors in the lung tissue, thereby improving or treating acute lung injury caused by LPS.

This invention belongs to the field of medical device technology and discloses a porous self-expanding hydrogel vascular scaffold and its preparation method. The preparation method includes the following steps: Step 1: Coating a hydrogel solution onto the outer wall of a hollow inner tube of a mold; the hydrogel solution is obtained by dissolving hydrogel material in a solvent, and the volume concentration of the hydrogel material in the solvent is 10%~20%; Step 2: Injecting liquid nitrogen into the hollow structure of the inner tube, the liquid nitrogen creates a temperature gradient (lower inside, higher outside) on the hydrogel solution coated on the outer wall of the inner tube for directional freezing, followed by thawing and repeated freeze-thaw treatment; Step 3: Immersing the frozen inner tube in a salt solution for salting out, then immersing it in water, and finally demolding the hydrogel from the inner tube to obtain the porous self-expanding hydrogel vascular scaffold. The hydrogel vascular scaffold prepared by this method can maintain the necessary material exchange capacity while preventing cell infiltration and excessive growth of tissue inside the scaffold.

The application belongs to the technical field of new application of medicines, and particularly relates to hydroxycinnamic acid derivatives, a preparation method and application thereof. The ARDS model is induced by LPS tracheal instillation combined with hydroxycinnamic acid derivatives, the alveolar lavage fluid cell count is determined, and the pathological characteristics and the expression level of inflammatory genes of lung tissues are detected; the results show that the hydroxycinnamic acid derivatives can significantly reduce lung cell infiltration, alleviate lung tissue damage and reduce the expression level of inflammatory factors.