Double-layer guided tissue regeneration membrane and preparation method thereof
A double-layer technology for guiding tissue regeneration, applied in the field of double-layer guiding tissue regeneration membrane and its preparation, can solve the problems of poor bone regeneration, uneven distribution of fibers, and inability to be absorbed by tissues
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[0061] The present invention provides a method of preparing a bilateral guiding tissue regeneration film in a second aspect, the method comprising the steps of:
[0062] S1, the preparation step of the loose layer: the type I collagen is made into collagen gel fluid, and then the loose layer is obtained by freeze drying;
[0063] S2, the preparation step of the dense layer: mix the polycaprolactone solution and mixed with the chitosan solution to obtain a PCL / CS electrospun solution, with the PCL / CS electrospun liquid as a spinning liquid through electrostatic spinning method in step The surface of the loose layer obtained by S1 is obtained, thereby obtaining a double composite film composed of a loose layer and a dense layer;
[0064] S3, crosslinking the double-layer composite film obtained by step S2 to obtain a double-layer guiding tissue regeneration film.
[0065]The present invention constructs a guide tissue regenerated film prepared by electrostatic spinning method. O...
Example Embodiment
[0125] Example 1
[0126] Step S1, the preparation of the loose layer, specifically includes:
[0127] Step S1-1, the I-type collagen is dissolved in purified water, and the collagen gel solution is formulated, wherein the collagen has a mass concentration of 0.1%;
[0128] Step S1-2, the collagen gel solution obtained from S1-1 is placed in a polytetrafluoroethylene plate, and the freeze dryer is placed in a cryogenic dryer, and the freeze drying process is as follows:
[0129] (1) The secondary partition is lowered to -10 ° C;
[0130] (2) The end point is determined to be ≤0.9Pa / 10min; the end point determination method: once the manual end point is performed every 10 minutes, the consecutive judgment is 3 times; the specific operation steps of freeze drying are carried out in Table 1 according to the present invention.
[0131] Step S1-3: Tuen the slotted collagen sponge prepared by S1-2 is trimmed and fixed to a cylindrical rotary receiver coated with an aluminum foil.
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Example Embodiment
[0151] Example 2
[0152] Example 2 is basically the same as that of Example 1, the difference is:
[0153] In step S2-1, 2.4 mL of poly (ε-caprolactone) solution, 1 mL of chitosan solution, mixed mix well, prepare PCL / CS electrospinning, spare; where, poly (ε-own The mass ratio of the chitosan contained in the inner deteric) solution and the chitosan contained in the chitosan solution is 4.5: 1.
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