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Method for preparing DL-alanine and D-alanine

A technology of alanine and aspartic acid, applied in the chemical field, can solve the problem of high production cost, and achieve the effects of reducing the processing capacity, improving the overall yield and economic benefits

Active Publication Date: 2016-06-01
宜兴市前成生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production method in the prior art uses L-alanine as raw material, and the production cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: prepare DL-alanine

[0023] In the coupled reactor, fumaric acid and ammonia synthesize L-aspartic acid through aspartase, and the synthesized L-aspartic acid obtains L-alanine through L-aspartate decarboxylase, so The above-mentioned L-alanine is racemized by alanine racemase to obtain DL-alanine; the structure and usage method of the coupling reactor are well known to those skilled in the art. Add 500 g of fumaric acid and 1500 ml of water (the mass percent concentration of the fumaric acid aqueous solution is 25%) in a 5 L beaker, and feed ammonia to adjust the pH to 7.5. It is pumped into the coupling reactor through a peristaltic pump and circulated at the same time. During the reaction process, the residues of fumaric acid and L-aspartic acid in the reaction solution and the optical rotation of the solution were detected. When fumaric acid residues < 0.5%, L-aspartic acid residues < 0.5%, and the optical rotation of the reaction solution is equal t...

Embodiment 2

[0024] Embodiment 2: Preparation of DL-alanine

[0025] In the coupled reactor, fumaric acid and ammonia synthesize L-aspartic acid through aspartase, and the synthesized L-aspartic acid obtains L-alanine through L-aspartate decarboxylase, so The above-mentioned L-alanine is racemized by alanine racemase to obtain DL-alanine; the structure and usage method of the coupling reactor are well known to those skilled in the art. Add 500 g of fumaric acid and 1500 ml of water into a 5 L beaker, and feed ammonia to adjust the pH to 7.3. It is pumped into the coupling reactor through a peristaltic pump and circulated at the same time. During the reaction process, the residues of fumaric acid and L-aspartic acid in the reaction solution and the optical rotation of the solution were detected. When fumaric acid residues < 0.5%, L-aspartic acid residues < 0.5%, and the optical rotation of the reaction solution is equal to "0", the reaction is stopped, and the reaction solution is heated ...

Embodiment 3

[0026] Embodiment 3: prepare D-alanine

[0027] The mother liquor collected by reaction concentration and centrifugation in Example 1 is split in a fermenter to obtain D-alanine through L-alanine transaminase; the specific process is: 2500ml of mother liquor (DL- Alanine concentration is about 16%), add 5L fermentor fermentation as fermentation ingredient water.

[0028] Fermentation medium: peptone 1%; yeast extract 1%; glucose 0.5%; sodium chloride 0.1%; corn steep liquor powder 1.2%; MgSO 4 0.01%; KH 2 PO 4 0.1%. Sterilize at 121°C for 20 minutes.

[0029] Fermentation temperature: 30°C, air volume: 8L / min. During the fermentation process, the pH was maintained at 5.6±0.1, and the pH was adjusted with 10% dilute sulfuric acid.

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Abstract

The invention discloses a method for preparing DL-alanine and D-alanine. Fumaric acid and ammonia react through aspartase in a coupling reactor to synthesize L-aspartic acid; the synthesized L-aspartic acid reacts with L-aspartate dacarboxylase to obtain L-alanine; L-alanine undergoes racemization through alanine racemase to obtain DL-alanine; and DL-alanine concentrated mother liquor undergoes splitting through L-alanine aminotransferase in a fermentation cylinder so as to obtain D-alanine. Production of DL-alanine or D-alanine requires no extraction of intermediate products. The final product is obtained directly by one step. Thus, usage of sulfuric acid required for electroextraction of L-aspartic acid and the like, labor for each extraction process, energy consumption and production loss such as dissipation in actual production process are reduced. Meanwhile, DL-alanine mother liquor is comprehensively utilized for production of D-alanine, and overall yield and economic benefit are raised.

Description

Technical field: [0001] The invention belongs to the field of chemical industry, and in particular relates to a method for preparing DL-alanine and D-alanine. technical background: [0002] DL-alanine is an important intermediate in the synthesis of some pesticides and pharmaceuticals, and it is also used in medical microbiology and biochemical amino acid metabolism. It can also be used as food flavoring agent, nutritional supplement, vitamin B6 intermediate, feed additive, etc., and can also be used as a biochemical reagent. D-alanine is an important organic chiral source, which is mainly used in the fields of chiral drugs, chiral additives, chiral auxiliary agents, etc., and is used as a chiral source for chiral synthesis in the pharmaceutical and food industries. As an optically active organic acid, it plays an irreplaceable role in the asymmetric synthesis of certain chiral compounds. It is currently mainly used for the production of new broad-spectrum antibiotics, D-al...

Claims

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Application Information

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IPC IPC(8): C12P13/06C12P41/00
Inventor 席日新刘涛
Owner 宜兴市前成生物有限公司
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