31 results about "Aspartate decarboxylase" patented technology

A high-throughput screening method for an L-aspartate beta-decarboxylase producing strain is disclosed. The method includes 1) subjecting microorganisms in a sample to enrichment culture and primary separation by adopting an enrichment culture medium, 2) inoculating single colonies obtained through separation from a plane plate to a 96-micropore plate primary screening semisolid medium and performing primary screening to obtain L-aspartate beta-decarboxylase producing strains, 3) inoculating the strains obtained through primary screening to a 24-prore plate, performing liquid culturing, and performing secondary screening to obtain the L-aspartate beta-decarboxylase producing strain with high yield by combining paper chromatography, and 4) subjecting the high-yield strain obtained through secondary screening to fermentation in a shake flask, and determining enzyme activity by combining an oxidation coloration process. The method is suitable for high-throughput screening of the L-aspartate beta-decarboxylase producing strain. The strain obtained through screening is alkali-resistant, thus avoiding tedious pH value controlling steps in a cell production processes. References are provided for high-throughput screening of producing strains bio-enzymes of the same kind.

The invention relates to a panD mutant gene, encoded protein, a vector, an engineering bacterium and an application of the encoded protein. Series recombinant escherichia coli, for high-yield production of L-aspartate-[alpha]-decarboxylase (PanD), is obtained by changing codon base of the panD gene through random mutation of error-prone PCR. With the application of gene engineering bacterium for high-yield production of the PanD, the efficient transformation of the L-aspartate is achieved, so that [beta]-alanine can be prepared by virtue of a biological method, wherein PanD enzyme activity of recombinant escherichia coli numbered as 6-85 can reach 250U / L or above and [beta]-Ala yield can reach about 8.5g / L, nearly 4 times and 3 times above that of a parent strain.