2 results about "Aspartate decarboxylase" patented technology

The invention provides a spatial regulation and control system, a beta-alanine production strain and a construction method and application thereof, the spatial regulation and control system comprises a recombinant protein RIDD-RGG-RGG and a recombinant protein aspC-RIAD-RIAD-panD. According to the strain, adhE, ldhA, ackA, poxB, lacI, thrA, panC, cycA and yfbQ genes are knocked out on a wild escherichia coli genome, and the recombinant protein aspC-RIAD-RIAD-panD is obtained. A beta-alanine transporter gene NCgl0580 derived from corynebacterium glutamicum is introduced in a heterologous manner, a phosphoenolpyruvate carboxylase gene ppc is over-expressed, a spatial dynamic regulation and control system containing an aspartate transaminase gene aspC and an aspartate decarboxylase gene panD is integrated, and the synthesis efficiency and the production intensity of the obtained strain are remarkably improved.

This invention relates to the field of microbial metabolic engineering, and particularly to a recombinant *Halomonas* strain that synthesizes P3HB3HP from glucose, its construction method, and its applications. This invention constructs a synthetic pathway in *Halomonas* containing enzymes such as aspartate decarboxylase and 4-aminobutyrate aminotransferase. The resulting recombinant *Halomonas* strain can synthesize P3HB3HP with a high 3HP ratio from glucose alone via the β-alanine pathway, while maintaining a high P3HB3HP yield. The recombinant *Halomonas* strain provided by this invention can produce P3HB3HP using inexpensive carbon sources, significantly reducing substrate costs and achieving high cell density, high yield, high 3HP ratio, and stable P3HB3HP synthesis.