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Method for preventing moss biological weathering on sandstone by using alcaligenes

A technology of sandstone and alkali-producing bacteria, which is applied in the fields of cultural relics protection and environmental science and engineering applications, can solve the problems of limitation, fading, and harm.

Active Publication Date: 2022-04-12
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the limitations, hazards, shedding and discoloration of mechanical repair, chemical killing, and bionic reinforcement materials in the prior art, the present invention proposes a method for preventing the biological weathering of moss on sandstone by using alcaligenes

Method used

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  • Method for preventing moss biological weathering on sandstone by using alcaligenes
  • Method for preventing moss biological weathering on sandstone by using alcaligenes

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Embodiment 1

[0023] Re-stretch the preserved Achromobacter xylosoxidans bacteria solution, pick a single colony after purification, add inorganic salt re-screening medium after expansion, cultivate until the pH of the bacteria solution is 8.5, adjust the OD value to 0.6, add Centrifuge the bacterial centrifuge tube at 5000 rpm for 10 minutes to remove the bacterial cells and take the supernatant, which is the crude extraction of extracellular enzymes, and put it into a clean spray bottle with a nozzle. On the moss-covered rock wall in Leshan Giant Buddha Scenic Area, Sichuan, use a sterilized spatula to clean up two square areas with a range of 12cm×12cm, remove the moss growing on the upper part, and clean the boundary and residual organisms with a brush to expose the lower part For red sandstone, the crude extract of Alcaligenes extracellular enzyme prepared by evenly spraying on the sandstone surface, the spraying rate is 0.1L / m 2 , a nearby area was sprayed with sterile water as a blan...

Embodiment 2

[0026] Re-stretch the preserved Achromobacter xylosoxidans bacteria solution, pick a single colony after purification, add inorganic salt re-screening medium after expansion, cultivate until the pH of the bacteria solution is 8.0, adjust the OD value to 1.0, add Centrifuge the bacterial centrifuge tube at 5000 rpm for 10 minutes to remove the bacterial cells and take the supernatant, which is the crude extraction of extracellular enzymes, and put it into a clean spray bottle with a nozzle. On the moss-covered rock wall in Leshan Giant Buddha Scenic Area, Sichuan, use a sterilized spatula to clean up two square areas with a range of 12cm×12cm, remove the moss growing on the upper part, and clean the boundary and residual organisms with a brush to expose the lower part For red sandstone, the crude extract of Alcaligenes extracellular enzyme prepared by evenly spraying on the sandstone surface, the spraying rate is 0.1L / m 2 An area next to it was sprayed with sterile water as a b...

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Abstract

The invention belongs to the fields of cultural relic protection and environmental science and engineering application, and discloses a method for preventing moss biological weathering on sandstone by using alcaligenes, which comprises the following steps: screening autogenous alcaligenes of red sandstone stone cultural relics, extracting an extracellular enzyme preparation, uniformly spraying on the rock surface of the stone cultural relics without moss growth, and carrying out fermentation for 1-2 hours. The method is used for regulating and controlling acid-producing weathering bacteria growing in moss, and the effect of preventing moss biological weathering is achieved from the microenvironment. Wherein the alkali-producing strain achromobacter xylosoxidans contains various decarboxylase genes such as aspartic acid decarboxylase and arginine decarboxylase, biological enzymes can be synthesized, biogenic amines can be generated by utilizing amino acids, the biogenic amines and organic acids at the roots of moss can be subjected to dehydration condensation reaction to generate near-neutral amide and water, and then the moss can be subjected to hydrolysis reaction to generate the xylosoxidans. Further, the acidic weathering environment on the sandstone surface is neutralized. According to the method, a green biological method is adopted, the method is friendly to cultural relics and the environment, and moss biological weathering on the gravel cultural relics can be prevented.

Description

technical field [0001] The invention belongs to the fields of cultural relics protection and environmental science and engineering application, and in particular relates to a method for preventing biological weathering of moss on sandstone by using alcaligenes. Background technique [0002] Sandstone cultural relics are the most widely distributed type of important stone cultural relics in my country. 80% of the cave temple cultural relics in my country are made of sandstone, including Leshan Giant Buddha, Yungang Grottoes, Dazu Rock Carvings, etc., which have rich cultural and artistic value. However, due to the large volume of stone cultural relics, most of them are immovable and are kept in the open air. They are exposed to wind and sun all the year round, and are seriously damaged by natural weathering. A large number of scholars have observed microbial colonization on the surface and interior of cultural relics: Gleeson et al. found fungal growth on the surface of weat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C04B41/46C12N1/20C12R1/025
Inventor 陈学萍刘泮坤陆永生黄继忠
Owner SHANGHAI UNIV
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