Production method of D-aspartic acid
A technique for producing aspartic acid and its production method, which is applied in the field of amino acid production and can solve the problems of low purity of D-aspartic acid, complex process, and low product yield.
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[0026] Preparation of DL-aspartic acid solid: take deionized water and add DL-aspartic acid by-product of aspartame, add liquid caustic soda to dissolve and make a solution with a concentration of 25-30% by mass, add liquid caustic soda Adjust the pH to 7.5-8.5, add aspartic acid racemase, heat up to 70-80°C for reaction, and end the reaction when the material ratio of the isoelectric crystallization in the central control is 0. After decolorization and filtration, ceramic membrane filtration, the temperature of the isoelectric crystallization is cooled to Below 30°C, centrifuge to separate DL-aspartic acid solid for future use.
[0027] Preparation of the reaction base material DL-aspartic acid solution: take deionized water and add L-aspartic acid, dissolve the liquid caustic soda to make a solution with a concentration of 25% to 30% by mass, add liquid caustic soda to adjust the pH to 7.5~ 8.5, add aspartic acid racemase, heat up to 70-80°C for reaction, end the reaction wh...
Embodiment 1
[0031] The present embodiment provides a kind of production method of D-aspartic acid, comprises steps:
[0032] Take 3000L of fermentation broth containing L-aspartic acid-β decarboxylase, the wet weight of bacteria in the fermentation broth is 35g / L, take 3000L of reaction bottom material DL-aspartic acid solution, bottom material DL-aspartic acid The concentration of DL-aspartic acid in the solution is 0.7g / ml, and the fermentation broth is added to the reaction bottom material, the concentration of DL-aspartic acid in the obtained reaction system is 0.35g / ml, and the reaction system is reacted at 45°C for 2h;
[0033] Then start feeding DL-aspartic acid solid, control the reaction pH not less than 6.0, when the total amount of solid feeding reaches 2500kg, stop feeding solid;
[0034] Then add 3000L reaction base material to the reaction system at one time, the concentration of DL-aspartic acid in the base material DL-aspartic acid solution is 0.7g / ml, and react for 3h;
...
Embodiment 2
[0039] The present embodiment provides a kind of production method of D-aspartic acid, comprises steps:
[0040] Take 3000L of fermentation broth containing L-aspartic acid-β decarboxylase, the wet weight of bacteria in the fermentation broth is 35g / L, take 3000L of reaction bottom material DL-aspartic acid solution, bottom material DL-aspartic acid The concentration of DL-aspartic acid in the solution is 0.6g / ml, the fermentation broth is added to the reaction bottom material, the content of DL-aspartic acid in the obtained reaction system is 0.3g / ml, and the reaction system is reacted at 45°C for 2h ;
[0041] Then start feeding DL-aspartic acid solid, control the reaction pH not less than 6.0, when the total amount of solid feeding reaches 2000kg, stop feeding solid;
[0042] Then add 2000L reaction base material to the reaction system at one time, the concentration of DL-aspartic acid in the base material DL-aspartic acid solution is 0.6g / ml, and react for 2h;
[0043] C...
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