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Method for preparing L-aspartic acid and L-alanine by using maleic anhydride

A technology of aspartic acid and alanine, applied in the direction of fermentation, can solve the problems of inability to further reduce costs, high production costs and high technical requirements, and achieve the effects of product diversification, good product quality and simple process

Inactive Publication Date: 2017-05-31
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process requires a lot of investment in equipment and high technical requirements, which makes the production cost of the product relatively high
In recent years, with the increasingly fierce market competition, how to effectively reduce production costs and improve product quality has become the key; and the immobilized enzyme method cannot further reduce costs due to its own technological limitations.

Method used

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  • Method for preparing L-aspartic acid and L-alanine by using maleic anhydride
  • Method for preparing L-aspartic acid and L-alanine by using maleic anhydride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 L-aspartic acid

[0041] 1) Maleic anhydride and pure water were stirred and dissolved in a 60°C water bath, and prepared into a maleic acid solution with a concentration of 60% for later use.

[0042] 2) Add the maleic acid solution obtained in step 1) into a 50L enzyme reaction kettle, adjust the reaction temperature to 40°C, adjust the pH value with 30% ammonia water, and control it at 7.0-9.0, as the reaction substrate. Calculated according to the volume of the reaction substrate, add 8% maleate isomerase enzyme solution and 10% L-aspartase enzyme solution to react, react at 40°C for 12h, and heat the conversion solution at the end of the reaction to 80°C Keep it for 30min.

[0043] 3) The enzyme reaction liquid obtained in step 2) is filtered with a ceramic membrane with a pore size of 0.05-0.2 μm, the membrane inlet pressure is 0.3 MPa, the membrane outlet pressure is 0.15 MPa, and the operating temperature is 60° C., and the filtr...

Embodiment 2

[0049] The preparation of embodiment 2 L-aspartic acid

[0050] 1) Maleic anhydride and pure water were stirred and dissolved in a water bath at 60°C, and prepared into a maleic acid solution with a concentration of 15% for later use.

[0051] 2) Add the maleic acid solution obtained in step 1) into a 50L enzyme reaction kettle, adjust the reaction temperature to 40°C, adjust the pH value with 30% ammonia water, and control it at 7.0-9.0, as the reaction substrate, calculated according to the volume of the reaction substrate, Calculated according to the volume of the reaction substrate, add 10% maleate isomerase enzyme solution and 15% L-aspartase enzyme solution respectively for reaction, react at 40°C for 10h, and heat the conversion solution at the end of the reaction to 105°C. Keep for 30min to inactivate.

[0052] 3) The enzyme reaction solution obtained in step 2) is filtered with a ceramic membrane with a pore size of 0.05-0.2 μm, the membrane inlet pressure is 0.2 MPa...

Embodiment 3

[0058] The preparation of embodiment 3 L-aspartic acid

[0059] 1) Maleic anhydride and pure water were stirred and dissolved in a 60°C water bath, and prepared into a maleic acid solution with a concentration of 60% for later use.

[0060] 2)) Add the maleic acid solution obtained in step 1) into a 50L enzyme reactor, adjust the reaction temperature to 40°C, adjust the pH value with 30% ammonia water, and control it at 7.0-9.0, as the reaction substrate, calculated according to the volume of the reaction substrate According to the volume of the reaction substrate, add 8% maleate isomerase enzyme solution and 10% L-aspartase enzyme solution to react, react at 40°C for 16h, and heat the conversion solution at the end of the reaction to 90°C And maintain 60min inactivation.

[0061] 3) The enzyme reaction solution obtained in step 2) is filtered with a ceramic membrane with a pore size of 0.05-0.2 μm, the membrane inlet pressure is 0.3 MPa, the membrane outlet pressure is 0.2 M...

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Abstract

The invention provides a method for preparing L-aspartic acid and L-alanine by using maleic anhydride. The method comprises the following steps: heating the maleic anhydride as the raw material to obtain a maleic acid solution through hydration reaction; adding maleate isomerase and L-aspartase; after filtering and concentrating obtained liquid of enzymatic reaction to deaminize, regulating the pH value until the isoelectric point of the L-aspartic acid is equal to 2.8 by using the maleic acid solution as an acidifying agent; crystallizing to separate out the L-aspartic acid; and converting the L-aspartic acid into the L-alanine by further using aspartate decarboxylase. The L-aspartic acid is prepared through one-step reaction by a two-enzyme method, the process is simple and convenient, the conversion ratio is high, production of malic acid as a by-product can be controlled effectively, and the quality of the product is good; and maleic acid is used as the acidifying agent to prepare the L-aspartic acid in an isoelectric point crystallization manner, crystallization mother liquor can be used as an enzyme reaction substrate and then is recycled, the discharge amount of waste water is greatly reduced, and the method conforms to the idea of environmental protection. In addition, the L-aspartic acid is further converted into the L-alanine by an enzymic method, so that products obtained by a whole process are diversified; and the market competitiveness is high.

Description

technical field [0001] The invention relates to the field of biochemical industry, in particular to a method for preparing L-aspartic acid and L-alanine by utilizing maleic anhydride. Background technique [0002] L-alanine is white crystal or crystalline powder, no peculiar smell, with sweet taste. Stable chemical properties, easily soluble in water (16.5%, 25°C), insoluble in ether or acetone. The decomposition temperature is 297°C, the isoelectric point is 5.79, and pKa -COOH =2.34, pKa -NH3 =9.60, [α]25D+14.6 (C=0.5-2.0 g / ml, 5 mol / L HCl). The pH value of 5% L-alanine aqueous solution is 5.5-7.0. The chemical structural formula is: [0003] [0004] The molecular formula of L-alanine is C 3 h 7 o 2 N, molecular weight 89.09, including two isomers of α-alanine and β-alanine. [0005] L-alanine is a non-essential amino acid for the human body, which is formed by transferring the amino group of glycine to pyruvate in the living body. During the glucose-alanine ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/20C12P13/06
CPCC12P13/06C12P13/20
Inventor 张毅张飞冯杰潘声龙马德金杨为华穆晓玲张雪锋
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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