5 results about "Enzyme reactor" patented technology

The present application relates to the technical field of lutein extraction, and provides a green extraction method of lutein from marigold, which comprises the following steps: mixing marigold dry flower particles with deionized water to prepare slurry, and performing high-pressure homogenization treatment to obtain pretreated slurry; mixing the pretreated slurry with a deep eutectic solvent (DES) and performing countercurrent extraction under the protection of inert gas to obtain DES extraction liquid after filtration; preheating the DES extraction liquid and pumping it into a continuous flow fixed-bed enzyme reactor filled with immobilized lipase, and performing enzymolysis reaction under constant temperature to obtain an enzymolysis liquid; sequentially treating the enzymolysis liquid through a ceramic ultrafiltration membrane and a nanofiltration membrane; collecting the retentate of the nanofiltration membrane, adding preheated deionized water to the nanofiltration retentate, and performing low-temperature standing crystallization; and washing and vacuum freeze-drying the obtained crystals to obtain a lutein crystal product. Through the above technical scheme, the extraction rate of lutein is improved, and the problem of incomplete extraction caused by insufficient cell structure damage in the traditional extraction method is improved.

This invention relates to the construction of novel enzyme reactors using amorphous porous organic polymers (POPs) for in-situ immobilization of enzyme molecules under room temperature aqueous conditions, thereby obtaining a highly efficient and inexpensive heterogeneous catalyst. This invention is the first attempt to immobilize enzymes in situ using amorphous porous organic polymer materials, broadening the scope of enzyme immobilization systems and providing valuable guidance for the industrial application of immobilization systems. The various azo-linked POPs involved in this invention are composed of a series of organic monomers linked by covalent bonds, exhibiting excellent stability and enabling high enzyme loading capacity and effective protection.

The application relates to the technical field of functionalized magnetic nanometer materials, and provides a DNA tetrahedron-based magnetic immobilized enzyme reactor, a preparation method and application of the magnetic immobilized enzyme reactor. The preparation method comprises the following steps: preparing magnetic graphene oxide, loading gold nanoparticles on the magnetic graphene oxide; synthesizing a DNA tetrahedron containing a mercapto group at a vertex by using a self-assembly reaction; modifying the DNA tetrahedron on the gold nanoparticles by using the covalent combination between Au and S; and finally loading trypsin on the DNA tetrahedron to obtain the immobilized enzyme reactor. The immobilized enzyme reactor has a large specific surface area, can effectively improve the loading amount of trypsin, the magnetic matrix facilitates the rapid separation and recovery of the immobilized enzyme reactor, realizes the recovery and repeated use of the enzyme, effectively controls the distance between the enzymes by controlling the size of the DNA tetrahedron, effectively avoids the loss of enzyme activity caused by the self-digestion of the enzyme, and improves the repeated use life and enzymolysis efficiency of the immobilized enzyme.

The utility model belongs to the stevia sugar production technical field discloses a device for producing steviol glycoside D by using gene recombination bacteria, which comprises a seed tank, a fermentation tank, a first centrifuge, a homogenizer, a second centrifuge, an enzyme reactor, a ceramic membrane ultrafiltration device, a purification device, a third centrifuge and a drying device that are sequentially connected by pipelines, pumps and valves. The utility model can improve the yield and purity of steviol glycoside D and meet market demand.