37 results about "Enzyme reactor" patented technology

The invention relates to the technical field of temperature control, in particular to an enzyme reaction kettle self-adaptive temperature control system and method, and the system comprises a temperature detection module, a temperature control module, a heat conduction oil flow control module and an automatic regulation and control module; temperature data of a reaction kettle are collected in real time through a temperature detection module, a temperature control module adjusts parameters based on a PID algorithm and fuzzy logic to generate a control instruction, and a heat conduction oil flow control module adjusts flow by combining double-closed-loop cascade control with a feedforward compensation algorithm, so that thermal inertia lag is shortened. And the automatic regulation and control module generates optimized process parameters based on a reaction kinetic model and a genetic algorithm, and dynamically adjusts a temperature set value. The control method comprises the steps of temperature data acquisition, PID control instruction generation, conduction oil flow coordinated regulation, process parameter optimization and the like. The problems that in the prior art, temperature control precision is insufficient, conduction oil flow control is not accurate enough, and technological parameters cannot be automatically regulated and controlled are solved.

The invention discloses a liver protection gastrodia elata polyphenol extract as well as a preparation method and application thereof, and belongs to the technical field of natural product extraction. The method aims at solving the problems that in the prior art, gastrodia elata polyphenol extraction efficiency is low, and active ingredients are prone to being damaged by heat. According to the technical scheme, the method is characterized by comprising the following steps: mixing a gastrodia elata raw material with liquid nitrogen after being subjected to freezing and unfreezing circulating pretreatment, and performing cell wall breaking treatment through a high-voltage pulsed electric field; mixing the wall-broken raw material with a buffer solution, carrying out enzymolysis by virtue of an immobilized enzyme reactor, cooling the system after enzymolysis, and carrying out ultrahigh-pressure extraction; centrifugally filtering the extracting solution, purifying through a macroporous adsorption resin column, and collecting ethanol eluent; and finally, carrying out vacuum concentration and spray drying on the eluent to obtain the gastrodia elata polyphenol extract. According to the method, the polyphenol yield is effectively improved, the activity of the components is protected, and the obtained extract can be used for preparing liver protection medicines.

The invention discloses an immobilized enzyme reactor for preparing low-carbon-chain triglyceride, and relates to the technical field of bioreactors, the immobilized enzyme reactor comprises a reactor body, a bearing ball carrying enzyme, a feeding pipe, a discharging pipe, a conveying channel, a conveying mechanism and a collector; a filter pipe is arranged in the reactor body, the upper end of the filter pipe is communicated with the conveying channel through a feeding pipe, the lower end of the filter pipe is communicated with a collector through a discharging pipe, and the lower end of the collector is communicated with the conveying channel; unused bearing balls are contained in the collector, when the immobilized enzyme needs to be recycled, the vibrator on the filter pipe vibrates the filter pipe, the bearing balls in the filter pipe roll into the collector from the discharging pipe under the combined action of vibration and gravity, and the unused bearing balls in the collector are moved to the feeding pipe through the conveying mechanism and finally enter the filter pipe; an operator can open the cover plate of the collector and take out the used bearing balls from the discharge port, so that the effect of recycling the immobilized enzyme is realized.

The invention provides a preparation method of yak bone collagen peptide, a composition for promoting skeletal development and application of the composition, and relates to the technical field of health food. The preparation method comprises the following steps: carrying out ultrasonic pretreatment on yak bones, and degreasing through supercritical CO2 to obtain a degreased product; mixing the degreased product with a fishy smell removing agent to obtain a fishy smell removing product; the fishy smell removing agent is an aqueous solution of a plant extract; the plant extract is selected from at least one of amomum tsao-ko extract, turmeric extract, green tea extract or momordica grosvenori extract; the fishy smell removal product is subjected to an enzymatic hydrolysis reaction through an immobilized compound enzyme reactor, wherein the compound enzyme is prolyl endopeptidase, papain and ficin; obtaining a crude product; carrying out ultrafiltration by adopting a filter membrane with the molecular weight cut-off of 1kDa, and adsorbing through macroporous resin. The yak bone collagen peptide and the composition prepared by the invention can obviously improve the body length, promote the skeletal development and promote the secretion of growth hormone, and meet the growth requirements of children.

Disclosed herein are methods and devices for accelerating the substrate-enzyme reaction rate of sample preparation in biological treatment assays using compressed and mixed films containing predetermined ratios of biological substrates, such as polypeptides, proteins, nucleic acids, sugars or lipids, and enzymes.

The invention relates to the technical field of pure biodiesel preparation, and particularly discloses a method for continuously preparing pure biodiesel from kitchen waste oil based on immobilized enzyme. The invention discloses a method for continuously preparing pure biodiesel from kitchen waste oil based on immobilized enzyme. The method comprises the following steps: filtering, dehydrating and degumming the kitchen waste oil to obtain pretreated kitchen waste oil; mixing the pretreated kitchen waste oil with an acyl acceptor to obtain a reaction substrate; and feeding the reaction substrate into an enzyme reactor filled with the immobilized lipase catalyst, carrying out transesterification, collecting reaction effluent, and carrying out separation and purification to obtain pure biodiesel. According to the continuous production technology, separation of the catalyst and the reaction substrate is achieved, the catalyst can be recycled, the immobilized lipase catalyst prepared through the covalent binding method has high stability and activity, and the yield of pure biodiesel can still reach 84% or above after continuous operation exceeds 200 hours.

The utility model relates to technical field of enzyme reactor, concretely is sea cucumber peptide ultrasonic wave auxiliary enzymolysis reactor, including the reaction kettle, one ultrasonic generator is fixedly installed on the reaction kettle outer wall, one support frame is fixedly installed in the inside of reaction kettle near the top department, one fixed link is fixedly installed in the support frame bottom, the fixed link circumferential outer wall is embedded and is installed with a plurality of ultrasonic wave generation end heads. The utility model discloses through setting up ultrasonic generator on the reaction kettle outer wall and embedding multiple ultrasonic wave generation end heads on the inside fixed link, can efficiently produce ultrasonic wave energy and promote sea cucumber peptide enzymolysis reaction, and the rotary frame drives the stirring blade and the scraper rotation, not only realizes material stirring and makes the enzymolysis more uniform, also prevents material adhesion through the scraping of the scraping piece, and reaction efficiency and product quality are improved.

The present application belongs to the field of green catalysis and biosynthesis, and particularly relates to a natural wood column continuous flow enzyme reactor, a preparation method and application thereof. The present application utilizes the hierarchical pore structure of natural wood fibers, uses silane modification technology to prepare surface modified wood columns, and starts from key technologies such as natural wood column continuous flow enzyme reactor construction and operation control, fully develops the structural advantages of the surface modified wood columns, combines the multiple advantages of immobilized enzymes, and obtains the natural wood column continuous flow enzyme reactor which has the advantages of being cheap and easy to obtain, simple preparation, high flux, high mass and heat transfer efficiency, green and environmentally friendly, easy to scale, continuous and automatic, and the like, and can be suitable for homogeneous and heterogeneous reactions, and has high product quality and long-term stable operation.

The utility model provides a lipase reactor and relates to the technical field of lipase production. The lipase reactor comprises a kettle body and a kettle cover, the kettle cover is movably connected with the kettle body, a net barrel and a first stirring assembly are arranged in the kettle body, a second stirring assembly is arranged on the kettle cover, the first stirring assembly is located on the outer side of the net barrel, and the second stirring assembly is located on the inner side of the net barrel. A temperature sensor is arranged on the inner wall of the net barrel, and a plurality of heating units are evenly distributed on the outer wall of the kettle body in the axial direction. The lipase reactor can maintain the activity of lipase, promote the lipase to contact with raw materials, improve the production efficiency and ensure the product quality.

The utility model relates to the technical field of reactors, and discloses a lipase reactor for efficiently preparing sterol ester, which comprises a reaction box, the top of the reaction box is provided with an open slot, both sides of the open slot are provided with fixing columns, adjacent sides of the two fixing columns are provided with sliding chutes, sliding plates are fixedly connected in the two sliding chutes, and the sliding plates are arranged in the open slot. A motor is fixedly connected to the top of the sliding plate, the output end of the motor penetrates through the sliding plate to be fixedly connected with a stirring rod, a gear is fixedly connected to the outer portion of the upper portion of the stirring rod, electric heating plates are arranged in the two sides of the reaction box, and electric push rods are fixedly connected to the front and rear portions of the top of the reaction box; the output end of the electric push rod is fixedly connected with a cleaning assembly. According to the reaction kettle disclosed by the utility model, the fixed column, the sliding groove, the sliding plate, the motor, the gear, the stirring rod and the rack are matched to work, the motor drives the stirring rod to rotate so as to stir a reaction solution, and the stirring rod can move while stirring, so that the stirring effect is better.

The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 microns and being functionalized with an enzyme, such as trypsin. The enzyme-immobilized particles, and immobilized enzyme reactors thereof, can be used in methods for on-line protein digestion.

The utility model provides a multi-layer stirring enzymolysis tank, relates to the technical field of enzymolysis equipment, and aims to solve the problems that in the stirring process of the enzymolysis tank, the uniformity is poor, materials are easy to deposit at the bottom of the tank, the uniformity of enzymolysis is easy to influence, the enzymolysis reaction cannot be fully carried out, the quality of products is uneven, and the overall yield is reduced. Comprising an enzymolysis tank, a group of mounting frames are slidably mounted at the top of the enzymolysis tank, a group of stirring blades are fixedly mounted at the bottom of a rotating shaft, and four groups of stirring blades are fixedly mounted on a second shaft; under the action of the elastic pieces on the supporting rods, the stirring blades at the bottom of the rotating shaft are always in contact with the bottom of the enzymolysis tank, and the four groups of stirring blades on the second shaft move up and down in a reciprocating manner while the second shaft rotates, so that operation at different heights can be carried out in the enzymolysis tank; materials at different heights and in different areas are fully stirred, so that enzyme is fully contacted with a substrate, and the enzymolysis reaction speed is accelerated.

The present application relates to the technical field of lutein extraction, and provides a green extraction method of lutein from marigold, which comprises the following steps: mixing marigold dry flower particles with deionized water to prepare slurry, and performing high-pressure homogenization treatment to obtain pretreated slurry; mixing the pretreated slurry with a deep eutectic solvent (DES) and performing countercurrent extraction under the protection of inert gas to obtain DES extraction liquid after filtration; preheating the DES extraction liquid and pumping it into a continuous flow fixed-bed enzyme reactor filled with immobilized lipase, and performing enzymolysis reaction under constant temperature to obtain an enzymolysis liquid; sequentially treating the enzymolysis liquid through a ceramic ultrafiltration membrane and a nanofiltration membrane; collecting the retentate of the nanofiltration membrane, adding preheated deionized water to the nanofiltration retentate, and performing low-temperature standing crystallization; and washing and vacuum freeze-drying the obtained crystals to obtain a lutein crystal product. Through the above technical scheme, the extraction rate of lutein is improved, and the problem of incomplete extraction caused by insufficient cell structure damage in the traditional extraction method is improved.

The invention relates to the technical field of bioengineering, in particular to cellulose-graphene composite hydrogel, a preparation method thereof, an immobilized enzyme reactor for PFAS defluorination and a preparation method of the immobilized enzyme reactor. The preparation method of the cellulose-graphene composite hydrogel comprises the following steps: adding an aminated graphene dispersion liquid into a cellulose solution, and uniformly mixing to obtain a cellulose-graphene suspension; adding violuric acid into the cellulose-graphene suspension, uniformly mixing, and standing to obtain primary hydrogel; the primary hydrogel is sequentially subjected to deionized soaking and cleaning, and the cellulose-graphene composite hydrogel is obtained. The immobilized enzyme reactor provided by the invention has the characteristics of environmental protection and low cost, and provides an innovative biological solution for PFAS polluted water treatment.

The invention discloses preparation and application of a rotary splicing paper-based enzymolysis device, and belongs to the technical field of micro-fluidic paper chips. The method comprises the following steps: firstly, fixing nanogold on a micro-fluidic paper chip, and then assembling enzyme molecules on the surface of the nanogold modified by concanavalin A, thereby forming a directional enzyme bonding-nanogold mediated bioreactor, namely a micro-enzyme reactor. According to the micro-enzyme reactor, due to the high surface area of the nanoparticles and a directional immobilization strategy, the performance of the enzyme is expected to exceed that of a bioreactor prepared by a random bonding method. In addition, the enzyme is in butt joint with or separated from the color developing device in a rotary splicing manner, so that the operation convenience and the reusability of the enzyme are improved. The rotary splicing paper-based enzymolysis device designed by the invention is simple and convenient to operate and low in enzyme consumption, does not depend on a large-scale instrument, can be used for screening acetylcholin esterase inhibitors and tracing the activity of traditional Chinese medicinal materials, and provides a new device for drug screening.

This invention relates to the construction of novel enzyme reactors using amorphous porous organic polymers (POPs) for in-situ immobilization of enzyme molecules under room temperature aqueous conditions, thereby obtaining a highly efficient and inexpensive heterogeneous catalyst. This invention is the first attempt to immobilize enzymes in situ using amorphous porous organic polymer materials, broadening the scope of enzyme immobilization systems and providing valuable guidance for the industrial application of immobilization systems. The various azo-linked POPs involved in this invention are composed of a series of organic monomers linked by covalent bonds, exhibiting excellent stability and enabling high enzyme loading capacity and effective protection.

The present invention relates to enzymatic reactor cells and related methods of use, e.g., to produce a compound or product such as hydrogen peroxide by using an enzymatic reactor cell, wherein the enzymatic reactor cell includes a surface, a linker, and one or more enzymes.

The application relates to the technical field of functionalized magnetic nanometer materials, and provides a DNA tetrahedron-based magnetic immobilized enzyme reactor, a preparation method and application of the magnetic immobilized enzyme reactor. The preparation method comprises the following steps: preparing magnetic graphene oxide, loading gold nanoparticles on the magnetic graphene oxide; synthesizing a DNA tetrahedron containing a mercapto group at a vertex by using a self-assembly reaction; modifying the DNA tetrahedron on the gold nanoparticles by using the covalent combination between Au and S; and finally loading trypsin on the DNA tetrahedron to obtain the immobilized enzyme reactor. The immobilized enzyme reactor has a large specific surface area, can effectively improve the loading amount of trypsin, the magnetic matrix facilitates the rapid separation and recovery of the immobilized enzyme reactor, realizes the recovery and repeated use of the enzyme, effectively controls the distance between the enzymes by controlling the size of the DNA tetrahedron, effectively avoids the loss of enzyme activity caused by the self-digestion of the enzyme, and improves the repeated use life and enzymolysis efficiency of the immobilized enzyme.

The invention discloses a production system and a production method for preparing biodiesel by using a high-acid-value grease enzyme method, and belongs to the technical field of resource recycling. Comprising a reaction and preliminary separation section and a glycerin concentration section, the reaction and preliminary separation section comprises at least three stages of enzyme reactors which are sequentially connected in series, and the enzyme reactors are used for carrying out multi-stage continuous esterification reaction on high-acid-value oil and methanol under the catalysis of biological enzyme; each stage of enzyme reactor is provided with an external circulation cooling loop which comprises an enzyme reaction circulating pump and an enzyme reaction cooler; the outlet of the last-stage enzyme reactor is connected to a glycerol settling tank and is used for settling and separating the reaction mixture, the light phase of the glycerol settling tank is mixed oil and enters a mixed oil tank, and the heavy phase of the glycerol settling tank is dilute glycerol and enters a glycerol concentration section. According to the invention, the synthesis process of the biodiesel is simplified, the conversion rate of fatty acid methyl ester is improved, the investment cost of the biodiesel is reduced, the operation risk and the corrosion to equipment are reduced, and the energy consumption and the production cost are reduced.

The present invention relates to enzymatic reactor cells and related methods of use, e.g., to produce a compound or product by using an enzymatic reactor cell, wherein the enzymatic reactor cell includes a surface, a linker, and one or more enzymes.

The present invention relates to enzymatic reactor cells and related methods of use, e.g., to produce formic acid and / or formate by using an enzymatic reactor cell, wherein the enzymatic reactor cell includes a surface, a linker, and one or more enzymes.

The present disclosure provides a technique that is advantageous from the viewpoint of suppressing deterioration of an enzyme while continuously activating a coenzyme. A reaction device (1a) according to the present disclosure comprises an enzyme reactor (10), an enzyme (15), and an electrochemical reactor (20). The enzyme (15) is disposed inside the enzyme reactor (10). In the reaction device (1a), the enzyme reactor (10) and the electrochemical reactor (20) are configured such that a reaction liquid (R) circulates between the enzyme reactor (10) and the electrochemical reactor (20). The reaction liquid (R) contains a substrate and a coenzyme. The enzyme (15) is, for example, an oxidoreductase. The substrate is oxidized or reduced by the enzyme (15). The coenzyme restores the activity of the enzyme (15) after the enzyme has reacted with the substrate.

The invention belongs to the crossing field of a biological enzyme immobilization technology and natural product activity screening, and particularly relates to application of immobilized alpha-amylase based on a magnetic carrier in efficient screening of a guava source alpha-amylase inhibitor. The immobilized alpha-amylase is prepared by taking a magnetic metal organic framework material as a carrier and adopting a method of combining electrostatic adsorption with chemical crosslinking. The carrier has the advantages of superparamagnetism, high stability and high enzyme loading capacity, and still keeps more than 80% of activity after being repeatedly used for 7 times. According to the method, the immobilized enzyme reactor and the UHPLC-QTOF / MS technology are integrated, an integrated process of magnetic separation and enrichment, enzyme activity screening, accurate molecular weight identification and structural analysis is established, and sensitive detection of the pmol-level trace inhibitor can be achieved. The binding energy of the obtained inhibitor and alpha-amylase is lower than-9.2 kcal / mol, and the inhibition activity is remarkable. The screening efficiency and accuracy of the hypoglycemic active components in the natural product are greatly improved.

The application provides a preparation method of yak bone collagen peptide, a composition for promoting bone development and application thereof, and relates to the technical field of health foods.The preparation method comprises the following steps: after ultrasonic pretreatment of yak bone, supercritical CO2 is used for defatting to obtain a defatted product; the defatted product is mixed with a deodorizing agent to obtain a deodorized product; the deodorizing agent is an aqueous solution of a plant extract; the plant extract is at least one selected from the group consisting of Amomum tsao-ko extract, turmeric extract, green tea extract and momordica grosvenori extract; the deodorized product is subjected to enzymolysis reaction through a fixed immobilized enzyme reactor, the complex enzyme is prolyl endopeptidase, papain and ficin; a crude product is obtained; the crude product is subjected to ultrafiltration through a filter membrane with a molecular weight cut-off of 1kDa and is subjected to macroporous resin adsorption.The yak bone collagen peptide and the composition prepared by the method can significantly improve body length, promote bone development and promote the secretion of growth hormone, and meet the growth needs of children.

The present invention relates to enzymatic reactor cells and related methods of use, e.g., to produce formic acid and / or formate by using an enzymatic reactor cell, wherein the enzymatic reactor cell includes a surface, a linker, and one or more enzymes.

The present invention provides a combined production process and application of monoglyceride and diglyceride, belonging to the technical field of organic synthesis. The combined production process comprises the following steps: (1) dissolving reaction substrates in a certain volume of solvent, mixing uniformly to obtain a mixed substrate; (2) transferring the mixed substrate in step (1) into a packed bed enzyme reactor equipped with immobilized lipase, and reacting under vacuum conditions to obtain a reaction product; (3) subjecting the reaction product in step (2) to molecular distillation to separate and obtain monoglyceride and diglyceride; The present invention uses a mixed solution of tert-butanol and propylene glycol methyl ether as the solvent, and a mixture of microbial lipase - Candida rugosa and commercial immobilized lipase Lipozyme RM IM as the lipase system. By controlling their ratio and reaction temperature, the mixed enzyme can exhibit better catalytic efficiency and enzyme stability, thereby obtaining monoglyceride and diglyceride with high yield and high purity.

The application relates to a single-cell proteome sample processing method, which comprises the following steps: taking a single-cell sample, adding 0.01-0.2% non-ionic surfactant for ultrasonic lysis to obtain a pretreated sample; performing denaturation reduction alkylation treatment on the pretreated sample to obtain an alkylated sample; adding an immobilized enzyme reactor to the alkylated sample for enzymolysis for 0.5-4 hours, centrifuging to obtain supernatant, and obtaining a processed sample; and a preparation method of the immobilized enzyme reactor, which comprises the following steps: adding polyethyleneimine to amino polymer microspheres to obtain hydrophilic amino polymer microspheres after reaction; adding graphene oxide to the hydrophilic amino polymer microspheres to obtain graphene oxide-polymer microspheres after reaction; adding a protease solution containing benzalkonium to the graphene oxide-polymer microspheres to obtain the immobilized enzyme reactor after reaction.

The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 microns and being functionalized with an enzyme, such as trypsin. The enzyme-immobilized particles, and immobilized enzyme reactors thereof, can be used in methods for on-line protein digestion.

The present disclosure provides technology which is useful in terms of increasing the speed of a process for continuously activating a coenzyme to cause a plurality of types of enzyme reactions. The present disclosure provides a reaction device (1a) wherein a first enzyme reaction vessel (10a), a first electrochemical reaction vessel (20a), a second enzyme reaction vessel (10b), and a second electrochemical reaction vessel (20b) are disposed such that a reaction solution (R) passes and circulates therethrough in a specific order. The reaction solution (R) contains a first substrate, a second substrate, and a coenzyme. The first substrate is oxidized or reduced by a first enzyme (15a). The second substrate is oxidized or reduced by a second enzyme (15b). The first enzyme (15a) is stored inside the first enzyme reaction vessel (10a). The second enzyme (15b) is stored inside the second enzyme reaction vessel (10b). The first electrochemical reaction vessel (20a) and the second electrochemical reaction vessel (20b) electrochemically restore the activity of the coenzyme.

The application discloses a hollow mesoporous single-atom molybdenum nanozyme, a preparation method of the nanozyme and preparation and application of a nanozyme reactor based on the nanozyme, and belongs to the technical field of functional nano material preparation and biological application thereof. The novel hollow mesoporous single-atom molybdenum nanozyme (HMMo-SAzyme) is prepared for the first time, has a large internal space and a high specific surface area, can have a high peroxide enzyme activity and can be used as a carrier for loading natural enzymes. In addition, the large pore size is beneficial to the rapid transmission of a catalytic substrate in the HMMo-SAzyme. Meanwhile, glucose oxidase (GOx) is loaded on the hollow mesoporous single-atom molybdenum nanozyme and is wrapped with hyaluronic acid (HA) to construct a nanozyme reactor (HMMo / GOx@HA) with a broad-spectrum antibacterial effect, which can be used as a carrier for loading natural enzymes and drugs, can cause a cascade catalytic reaction at a bacterial infection site, can effectively inhibit bacterial infection of a wound and can promote wound healing.