12 results about "Enzyme reactor" patented technology

The utility model relates to technical field of enzyme reactor, concretely is sea cucumber peptide ultrasonic wave auxiliary enzymolysis reactor, including the reaction kettle, one ultrasonic generator is fixedly installed on the reaction kettle outer wall, one support frame is fixedly installed in the inside of reaction kettle near the top department, one fixed link is fixedly installed in the support frame bottom, the fixed link circumferential outer wall is embedded and is installed with a plurality of ultrasonic wave generation end heads. The utility model discloses through setting up ultrasonic generator on the reaction kettle outer wall and embedding multiple ultrasonic wave generation end heads on the inside fixed link, can efficiently produce ultrasonic wave energy and promote sea cucumber peptide enzymolysis reaction, and the rotary frame drives the stirring blade and the scraper rotation, not only realizes material stirring and makes the enzymolysis more uniform, also prevents material adhesion through the scraping of the scraping piece, and reaction efficiency and product quality are improved.

The utility model provides a lipase reactor and relates to the technical field of lipase production. The lipase reactor comprises a kettle body and a kettle cover, the kettle cover is movably connected with the kettle body, a net barrel and a first stirring assembly are arranged in the kettle body, a second stirring assembly is arranged on the kettle cover, the first stirring assembly is located on the outer side of the net barrel, and the second stirring assembly is located on the inner side of the net barrel. A temperature sensor is arranged on the inner wall of the net barrel, and a plurality of heating units are evenly distributed on the outer wall of the kettle body in the axial direction. The lipase reactor can maintain the activity of lipase, promote the lipase to contact with raw materials, improve the production efficiency and ensure the product quality.

The present application relates to the technical field of lutein extraction, and provides a green extraction method of lutein from marigold, which comprises the following steps: mixing marigold dry flower particles with deionized water to prepare slurry, and performing high-pressure homogenization treatment to obtain pretreated slurry; mixing the pretreated slurry with a deep eutectic solvent (DES) and performing countercurrent extraction under the protection of inert gas to obtain DES extraction liquid after filtration; preheating the DES extraction liquid and pumping it into a continuous flow fixed-bed enzyme reactor filled with immobilized lipase, and performing enzymolysis reaction under constant temperature to obtain an enzymolysis liquid; sequentially treating the enzymolysis liquid through a ceramic ultrafiltration membrane and a nanofiltration membrane; collecting the retentate of the nanofiltration membrane, adding preheated deionized water to the nanofiltration retentate, and performing low-temperature standing crystallization; and washing and vacuum freeze-drying the obtained crystals to obtain a lutein crystal product. Through the above technical scheme, the extraction rate of lutein is improved, and the problem of incomplete extraction caused by insufficient cell structure damage in the traditional extraction method is improved.

The invention discloses preparation and application of a rotary splicing paper-based enzymolysis device, and belongs to the technical field of micro-fluidic paper chips. The method comprises the following steps: firstly, fixing nanogold on a micro-fluidic paper chip, and then assembling enzyme molecules on the surface of the nanogold modified by concanavalin A, thereby forming a directional enzyme bonding-nanogold mediated bioreactor, namely a micro-enzyme reactor. According to the micro-enzyme reactor, due to the high surface area of the nanoparticles and a directional immobilization strategy, the performance of the enzyme is expected to exceed that of a bioreactor prepared by a random bonding method. In addition, the enzyme is in butt joint with or separated from the color developing device in a rotary splicing manner, so that the operation convenience and the reusability of the enzyme are improved. The rotary splicing paper-based enzymolysis device designed by the invention is simple and convenient to operate and low in enzyme consumption, does not depend on a large-scale instrument, can be used for screening acetylcholin esterase inhibitors and tracing the activity of traditional Chinese medicinal materials, and provides a new device for drug screening.

This invention relates to the construction of novel enzyme reactors using amorphous porous organic polymers (POPs) for in-situ immobilization of enzyme molecules under room temperature aqueous conditions, thereby obtaining a highly efficient and inexpensive heterogeneous catalyst. This invention is the first attempt to immobilize enzymes in situ using amorphous porous organic polymer materials, broadening the scope of enzyme immobilization systems and providing valuable guidance for the industrial application of immobilization systems. The various azo-linked POPs involved in this invention are composed of a series of organic monomers linked by covalent bonds, exhibiting excellent stability and enabling high enzyme loading capacity and effective protection.

The application relates to the technical field of functionalized magnetic nanometer materials, and provides a DNA tetrahedron-based magnetic immobilized enzyme reactor, a preparation method and application of the magnetic immobilized enzyme reactor. The preparation method comprises the following steps: preparing magnetic graphene oxide, loading gold nanoparticles on the magnetic graphene oxide; synthesizing a DNA tetrahedron containing a mercapto group at a vertex by using a self-assembly reaction; modifying the DNA tetrahedron on the gold nanoparticles by using the covalent combination between Au and S; and finally loading trypsin on the DNA tetrahedron to obtain the immobilized enzyme reactor. The immobilized enzyme reactor has a large specific surface area, can effectively improve the loading amount of trypsin, the magnetic matrix facilitates the rapid separation and recovery of the immobilized enzyme reactor, realizes the recovery and repeated use of the enzyme, effectively controls the distance between the enzymes by controlling the size of the DNA tetrahedron, effectively avoids the loss of enzyme activity caused by the self-digestion of the enzyme, and improves the repeated use life and enzymolysis efficiency of the immobilized enzyme.

The present invention relates to enzymatic reactor cells and related methods of use, e.g., to produce a compound or product by using an enzymatic reactor cell, wherein the enzymatic reactor cell includes a surface, a linker, and one or more enzymes.

The application provides a preparation method of yak bone collagen peptide, a composition for promoting bone development and application thereof, and relates to the technical field of health foods.The preparation method comprises the following steps: after ultrasonic pretreatment of yak bone, supercritical CO2 is used for defatting to obtain a defatted product; the defatted product is mixed with a deodorizing agent to obtain a deodorized product; the deodorizing agent is an aqueous solution of a plant extract; the plant extract is at least one selected from the group consisting of Amomum tsao-ko extract, turmeric extract, green tea extract and momordica grosvenori extract; the deodorized product is subjected to enzymolysis reaction through a fixed immobilized enzyme reactor, the complex enzyme is prolyl endopeptidase, papain and ficin; a crude product is obtained; the crude product is subjected to ultrafiltration through a filter membrane with a molecular weight cut-off of 1kDa and is subjected to macroporous resin adsorption.The yak bone collagen peptide and the composition prepared by the method can significantly improve body length, promote bone development and promote the secretion of growth hormone, and meet the growth needs of children.

The application relates to a single-cell proteome sample processing method, which comprises the following steps: taking a single-cell sample, adding 0.01-0.2% non-ionic surfactant for ultrasonic lysis to obtain a pretreated sample; performing denaturation reduction alkylation treatment on the pretreated sample to obtain an alkylated sample; adding an immobilized enzyme reactor to the alkylated sample for enzymolysis for 0.5-4 hours, centrifuging to obtain supernatant, and obtaining a processed sample; and a preparation method of the immobilized enzyme reactor, which comprises the following steps: adding polyethyleneimine to amino polymer microspheres to obtain hydrophilic amino polymer microspheres after reaction; adding graphene oxide to the hydrophilic amino polymer microspheres to obtain graphene oxide-polymer microspheres after reaction; adding a protease solution containing benzalkonium to the graphene oxide-polymer microspheres to obtain the immobilized enzyme reactor after reaction.

The application discloses a hollow mesoporous single-atom molybdenum nanozyme, a preparation method of the nanozyme and preparation and application of a nanozyme reactor based on the nanozyme, and belongs to the technical field of functional nano material preparation and biological application thereof. The novel hollow mesoporous single-atom molybdenum nanozyme (HMMo-SAzyme) is prepared for the first time, has a large internal space and a high specific surface area, can have a high peroxide enzyme activity and can be used as a carrier for loading natural enzymes. In addition, the large pore size is beneficial to the rapid transmission of a catalytic substrate in the HMMo-SAzyme. Meanwhile, glucose oxidase (GOx) is loaded on the hollow mesoporous single-atom molybdenum nanozyme and is wrapped with hyaluronic acid (HA) to construct a nanozyme reactor (HMMo / GOx@HA) with a broad-spectrum antibacterial effect, which can be used as a carrier for loading natural enzymes and drugs, can cause a cascade catalytic reaction at a bacterial infection site, can effectively inhibit bacterial infection of a wound and can promote wound healing.

The utility model belongs to the stevia sugar production technical field discloses a device for producing steviol glycoside D by using gene recombination bacteria, which comprises a seed tank, a fermentation tank, a first centrifuge, a homogenizer, a second centrifuge, an enzyme reactor, a ceramic membrane ultrafiltration device, a purification device, a third centrifuge and a drying device that are sequentially connected by pipelines, pumps and valves. The utility model can improve the yield and purity of steviol glycoside D and meet market demand.

The utility model relates to the technical field of reactors, and particularly discloses an immobilized enzyme reactor for bisoprolol synthesis, which comprises a reaction tank, a tank cover mounted at the top of the reaction tank and a bottom support mounted at the bottom of the reaction tank, a feed pipe is mounted at the top of the tank cover, a discharge pipe is mounted at the bottom of the bottom support, and the bottom support is fixed at the bottom of the reaction tank through a fastener. A plurality of positioning rods extending into the reaction tank are mounted at the top of the bottom support, a group of packed columns are mounted among the plurality of positioning rods, and limiting rings abutting against the tops of the packed columns are mounted on the inner wall of the reaction tank; the bottom support is fixed at the bottom of the reaction tank through the fastener, the packed column can be taken out by moving out the bottom support, the reaction tank does not need to be disassembled, the immobilized enzyme in the regenerated packed column is easily and quickly replaced, and the influence on the productivity in the synthesis of the sololol is reduced.