54 results about "Acetylhomocholine" patented technology

The invention discloses a preparation method of a micro-enzyme reactor based on magnetic functionalized graphene oxide, and application of the micro-enzyme reactor in pesticide detection, belonging to the technical field of micro-fluidic control chips. The micro-enzyme reactor which can be applied to pesticide detection is prepared by firstly, loading Fe3O4 magnetic nano particles on the surface of graphene oxide by using a one-step in-situ synthesis method, so as to prepare a graphene oxide/Fe3O4 nano composite material with both good magnetism and biocompatibility, secondly, fixing acetyl choline esterase on the surface of the graphene oxide/Fe3O4 through action of pi-pi, hydrogen bonds and water repelling and the like, and fixing a graphene oxide/Fe3O4/acetyl choline esterase compound inside a PDMS (Polydimethylsiloxane) micro chip channel through the action of an external magnetic field. Based on the inhibition principle of pesticide to acetyl choline esterase, the rapid quantitative detection on dimethoate is realized. The micro-enzyme reactor which is prepared based on a graphene oxide/Fe3O4/acetyl choline esterase composite material is rapid in action, simple to operate and good in reproducibility, and an effective means is provided for rapid and sensitive detection on pesticide.

The invention discloses an amino sugar thiazole derivative as well as a synthetic method thereof. When synthesis is carried out, glucosamine hydrochloride is taken as a raw material, and benzyl ether protection is carried out on hydroxy, so that the selective reaction of amino is realized, a novel intermediate glycosyl thiourea-N-(1,3,4,6-tetra-0-benzyl-2-deoxygenation- beta-D-glucopyranose-2-group) thiocarbamide is synthesized, and glycosyl thiazole-N-(1,3,4,6-tetra-0-benzyl-beta-D-glucopyranose-2-group)-2-amido-4-substituted thiazole is synthesized by cyclizing the novel intermediate glycosyl thiourea-N-(1,3,4,6-tetra-0-benzyl-2-deoxygenation beta-D-glucopyranose-2-group) thiocarbamide with 1-bromine-2-substituted ethyl ketone. The synthetic method disclosed by the invention has the advantages of easiness and safety for operation, wide application scope, low cost of the raw material, easiness for raw material obtaining, easiness and convenience for post-processing and high yield, is a fast high-efficiency synthetic method and has a wide application prospect on the aspect of preparing an acetylcholinesterase resistant drug because the synthesized compound has high inhibiting effect on acetylcholinesterase.

The invention provides a multi-target tacrine derivative and a preparation method and application thereof. The compound has the structure shown in a formula (I) (please see the specification in the formula (I)), wherein n is a natural number greater than 1; m is 1 or 2; p is 0 or 1; R1 is H or halogen; and Y is selected from the following structures (please see the specification for the structures) or, a pharmaceutically acceptable salt of a compound in a formula (X). According to the compound, activity of histone deacetylase (HDACs), acetylcholinesterase (AChE) or butyryl cholinesterase (BChE) can be effectively inhibited.

The invention relates to a 4-cyclamine alkoxy-3-methoxyl cinnamic acid benzamide compound, a preparation method and application of the compound.The chemical structure general formula of the compound is shown as a formula (I) (please see the formula in the description), wherein m represents 1-12, R1 represents C1-C12 alkyl or benzyl or substituted benzyl, X represents C1-C12 alkyl or benzyl or substituted benzyl, phenyl of which a benzene ring is substituted with 1-4 groups selected from F, Cl, Br, I, C(1-4) alkyl, C(1-4) alkoxy, trifluoromethyl, trifluoromethoxy, nitryl, a cyano group, and the substituent groups can be located at any position of the benzene ring.Accordingly, the compound has the significant inhibiting effect on acetylcholinesterase, the IC50 of the compound is 0.01-5 micromoles per liter, the inhibiting activity on acetylcholinesterase is greatly higher than the inhibiting activity on butyrylcholine esterase, and therefore the compound has the certain selective inhibiting effect on acetylcholinesterase.

The invention relates to the technical field of battery recycling, and particularly relates to a process for recycling waste lithium batteries. The method comprises the steps of preparing a deep eutectic solvent, specifically, mixing and heating quaternary ammonium salt and an amine compound to obtain the deep eutectic solvent, wherein the quaternary ammonium salt is selected from one or a mixtureof more of choline chloride, choline acetate and acetylcholine, and the amine compound is selected from one or a mixture of more of urea, ethanolamine and triethanolamine; and leaching, specifically,leaching the lithium cobalt oxide positive plate by using the eutectic solvent, and disassembling the positive plate from the waste lithium battery to obtain a metal-containing solution and an aluminum foil current collector. The method has the advantages of wide raw material source, low cost, simple process steps and the like.

The invention relates to carboline compounds, and a synthetic method and anti-acetylcholinesterase activity thereof. The carboline compounds have been proved to have a plurality of biological activities like anti-microbe activity, anticancer and anti-virus activity; however, researches on the anti-acetylcholinesterase activity and anti-cholinesterase activity have not been reported in the prior art. The carboline compounds provided by the invention are classified into a class A, a class B, a class C and a class D, wherein the class B and the class D are 2-aryl carboline salt compounds while the class A and the class C are 2-aryl-3,4-dihydrocarboline salt compounds. Results of activity determination show that the compounds provided by the invention have substantial anti-acetylcholinesterase and anti-cholinesterase activities and have great potential as active ingredients for preparation of drugs used for treating the Alzheimer disease.

The invention provides a biological sensor, a preparation method, application in detection of acetylcholin esterase, and a method for detecting an organophosphorus pesticide. The method is characterized in that acetyl choline has mimic enzyme to catalyze and oxidize TMB (3,3',5,5'-tetramethylbenzidine) to discolor, and can be decomposed and deactivated by the acetylcholin esterase, but the decomposing of hydrogen peroxide cannot be catalyzed, so that the TMB solution cannot generate color developing reaction; a linear relationship of absorbance and acetylcholin esterase is built, and the acetylcholin esterase is quantitatively detected; furthermore, the organophosphorus pesticide can effectively inhibit the activity of the acetylcholin esterase, the acetylcholin esterase is deactivated, the deactivated acetylcholin esterase cannot decompose the acetyl choline, and the TMB solution is promoted to generate the color developing reaction, thereby detecting the organophosphorus pesticide.

The invention provides acetylthiocholine iodide and a preparation method thereof. The preparation method comprises the following steps: step S1, dissolving a dimethylamino ethane compound in alkali toobtain an alkaline aqueous solution; step S2, adding a thioacetic acid compound into the alkaline aqueous solution to subject the thioacetic acid compound and the dimethylamino ethane compound to a thioreaction to generate (2-(dimethylamino)ethyl)ethanethioate; S3, separating the (2-(dimethylamino)ethyl)ethanethioate, and preparing an organic solution of the (2-(dimethylamino)ethyl)ethanethioate;and S4, dropwise adding an organic iodide into the organic solution of the (2-(dimethylamino)ethyl)ethanethioate for an iodination reaction so as to generate the acetylthiocholine iodide. According to the preparation method provided by the embodiment of the invention, the high-purity acetylthiocholine iodide can be obtained, and the titration content, the melting point, the clarity and other dataof the product are very excellent. The method is simple in operation, good in safety, suitable for industrial production, easy in raw material obtaining and low in cost.

The invention relates to the technical field of electrochemical analysis, in particular to an AchE/GQDs/N-F-TiO2Ns photoelectrochemical biosensor preparation method and application thereof. The AchE/GQDs/N-F-TiO2Ns photoelectrochemical biosensor comprises GQDs/N-F-TiO2Ns and AchE, wherein the GQDs/N-F-TiO2Ns comprises a nitrogen and fluorine-doped titanium dioxide nanotube array (N-F-TiO2Ns) and graphene quantum dots (GQDs) modified on the titanium dioxide nanotube array; cross-linking modification of acetyl cholinesterase (AchE) on the GQDs/N-F-TiO2Ns is carried out; the wavelength at the highest photocurrent response of the photoelectrochemical biosensor is 365 nm; and cross-linking modification of the AchE on the GQDs/N-F-TiO2Ns is carried out through chitosan. The modification of the graphene quantum dots improves the photocurrent intensity. Due to the good biocompatibility, the immobilization of the enzyme on an electrode is improved, and the affinity of the enzyme to a substrateis improved at the same time. the method can also be used in detection study related to acetylcholine and acetyl cholinesterase.

The invention discloses a method for detecting the content of pirimicarb in food by combining sulfur-doped carbon quantum dots with an enzyme inhibition method. The method comprises the following steps: S1, preparing pirimicarb standard solutions with different concentrations, and respectively adding the pirimicarb standard solutions into a crushed blank food sample; extracting by using water-soluble DES, adding acetylcholin esterase into an extracting solution, culturing, adding acetylthiocholine iodide, a pH buffer solution and S-CQDs, and culturing at constant temperature again; measuring fluorescence values of solutions with different concentrations, and drawing a standard curve; s2, extracting the crushed food to be detected by adopting water-soluble DES; s3, taking the extracting solution obtained in the step S2, and operating according to the step S1 to obtain the fluorescence intensity of the system; obtaining the fluorescence according to a standard curve and the measured fluorescence intensity. The extracting solution of the water-soluble DES is mutually soluble with a subsequent detection system, and the surface of the S-CQDs contains a large amount of negative charges which can generate electrostatic interaction with a hydrolysate thiocholine (+) of acetylthiocholine to cause fluorescence quenching; pirimicarb can inhibit enzyme activity, system fluorescence is enhanced, and quantitative detection is achieved.

The invention relates to a compound of formula I or pharmaceutically acceptable salt and a preparation method thereof, as well as a drug composition containing the same. The compound of formula I is novel piperazine and homopiperazine derivative, which can be used for preparing cervical cancer-treating medicine, anti-acetylcholinesterase and butyrylcholinesterase medicine, and medicine for treating staphylococcus aureus infection.

The invention discloses a preparation process of a gel combination with a sweating function, and relates to the technical field of gels, the technical scheme is as follows: the preparation process comprises preparation of sweating active gel and preparation of conductive solids, and the preparation of the sweating active gel comprises the following specific steps: S1, preparing the conductive solids; the preparation method comprises the following steps: dissolving pilocarpine or nitrate or choline receptor stimulant or organic matter and agar in deionized water according to a certain proportion, heating, stirring to fully dissolve the components after heating, pouring into a gel grinding tool after dissolving, standing, and cooling and solidifying; the preparation process of the gel combination with the sweating function has the beneficial effects that active substances can be excited in a current path to enter a body so as to achieve the sweating function, the gel combination is convenient, can be randomly adjusted, has no side effect on a human body and can be used for a long time, a product can be quickly metabolized by acetylcholine enzyme in the body, and the sweating function is realized. And the method is safe and effective.