61 results about "Acetylhomocholine" patented technology

This document provides methods and materials related to regulating inflammatory pathways. For example, compositions and kits containing two or more of an anticholinesterase compound, a choline compound, and a carnitine compound and methods for using the compositions and kits described herein to support acetylcholine function to regulate one or more inflammatory pathways are provided.

The invention relates to a preparation method and application of 9-bit substituent double-functional group berberine derivatives. In-vitro biological activity assay research shows that the 9-bit substituent double-functional group berberine derivatives provided by the invention has acetylcholinesterase inhibition activity higher than that of berberine serving as a leading compound, and also has higher inhibition activity on butyrylcholine esterase. After being further optimized and screened, the derivatives have favorable foreground of being developed into medicaments for treating diseases such as AD disease, vascular dementia, cognitive dysfunction and the like. The general formula of the derivatives is shown as the following formula, wherein X, Y and Z are defined in specifications.

The invention relates to a magnetic graphene enzyme-mimicking property-based acetylcholine visual-detection method, belonging to the technical field of biosensing. A hydrothermal method is used for synthesizing Fe3O4 / rGO by one step, H2O2 (hydrogen peroxide) is catalyzed to oxidize a substrate TMB (tetramethylabenzidine) to generate blue by utilizing the peroxidase-like property of the Fe3O4 / rGO, and the characteristic absorption intensity of an oxidation product of the TMB at the wavelength of 652nm is detected to realize the detection of hydrogen peroxide; furthermore, the H2O2 can be catalyzed to oxidize and convert the colorless TMB into a corresponding blue product based on the Fe3O4 / rGO, and the blue product is combined with acetyl choline to decompose H2O2 in the presence of both acetylcholinesterase and choline oxidase, and a novel acetylcholine visual-sensing method is established. The invention aims to provide an acetylcholine colorimetric detection method, and according to the method, the operation is convenient and flexible, the detection cost is low, the sensitivity is high, and instruments and equipment are simple.

The present invention discloses a preparation method of a test paper for testing the residual organphosphorous pesticide and a using method thereof, wherein the preparation method comprises the following procedures: (1) preparing acetylcholine esterase with fresh blood of hen or female duck as raw material; (2) fixing the acetylcholine esterase on the filter paper; (3) slicing when the filter paper is frozen and dried. A substrate liquid, a color developing liquid and a sensitization liquid are provided. The principle is that the inhibition of the organphosphorous pesticide which is processed by the sensitization liquid to the acetylcholine esterase in the chicken or duck blood serum to make that the substrate of naphthyl acetate of the acetylcholine esterase can not be decomposed thereby that the displayed colors are not consistent when the fast blue B is added into to obtain the purpose of determining whether the sample contains the residual organphosphorous pesticide. The method of the invention has the advantages of low cost, simple and fast operation. The method is suitable for determining whether the residual organphosphorous pesticide exists in vegetable, fruit and food on-site.

The invention discloses a chemiluminescence sensing detection method for organophosphorus pesticide residues and application thereof. The method is characterized by comprising the following steps: by taking a chemiluminescent probe as an indicator, indicating the organophosphorus pesticides to inhibit the degree of reducing choline produced by acetylcholine chloride subjected to catalytic hydrolysis of acetylcholin esterase, coupling choline oxidase to catalyze choline to generate hydrogen peroxide, then oxidizing a luminescence probe to generate chemiluminiscence by virtue of hydrogen peroxide, carrying out a multi-step coupling reaction to realize sensing detection of the organophosphorus pesticide residues by utilizing a chemiluminescence apparatus. According to the sensing method, 96 samples can be detected within 20 minutes, and dichlorvos is taken as an example, the detection limit reaches 1nmol / L. The method is sensitive and rapid and can be used for blood sample monitoring of patients suffering from poisoning of foods, water samples and organophosphorus poisons.

The invention relates to a method and a device for detecting pesticide by using cholinesterase. More specifically, the invention relates to a novel pesticide detection method based on acetylcholinesterase-fixed magnetic nanoparticles, wherein the magnetic nanoparticles serve as carriers to collect the acetylcholinesterase in solution. The invention also relates to a portable pen-shaped detector adopting the method for detecting pesticide.

The present invention discloses a trifluoromethyl containing quinazoline derivative and a preparation method and application thereof. The preparation method comprises the following steps of: under the condition that copper and ligand exist, an N-cyano substituted aryl carboxamides compound reacting with a togni reagent in a solvent, and after completion of the reaction, obtaining the trifluoromethyl containing quinazoline derivative after treatment. The reaction substrate is readily available, the operation is convenient, the obtained quinazolinone derivative is of rich diversity, the required reaction condition is mild, and the reaction time is short. The all-in-one-pot reaction is realized, and not only a structural mother kernel of quinazoline is obtained, but also trifluoromethyl is introduced into the mother kernel structure. And the quinazolinone compound contains the spiropyran compound can be synthesized, which cannot be achieved by the prior art. The obtained product has good activity in inhibiting enzyme acetylcholinesterase and butyrylcholinesterase.

The invention discloses a preparation method of a micro-enzyme reactor based on magnetic functionalized graphene oxide, and application of the micro-enzyme reactor in pesticide detection, belonging to the technical field of micro-fluidic control chips. The micro-enzyme reactor which can be applied to pesticide detection is prepared by firstly, loading Fe3O4 magnetic nano particles on the surface of graphene oxide by using a one-step in-situ synthesis method, so as to prepare a graphene oxide / Fe3O4 nano composite material with both good magnetism and biocompatibility, secondly, fixing acetyl choline esterase on the surface of the graphene oxide / Fe3O4 through action of pi-pi, hydrogen bonds and water repelling and the like, and fixing a graphene oxide / Fe3O4 / acetyl choline esterase compound inside a PDMS (Polydimethylsiloxane) micro chip channel through the action of an external magnetic field. Based on the inhibition principle of pesticide to acetyl choline esterase, the rapid quantitative detection on dimethoate is realized. The micro-enzyme reactor which is prepared based on a graphene oxide / Fe3O4 / acetyl choline esterase composite material is rapid in action, simple to operate and good in reproducibility, and an effective means is provided for rapid and sensitive detection on pesticide.

The invention discloses an amino sugar thiazole derivative as well as a synthetic method thereof. When synthesis is carried out, glucosamine hydrochloride is taken as a raw material, and benzyl ether protection is carried out on hydroxy, so that the selective reaction of amino is realized, a novel intermediate glycosyl thiourea-N-(1,3,4,6-tetra-0-benzyl-2-deoxygenation- beta-D-glucopyranose-2-group) thiocarbamide is synthesized, and glycosyl thiazole-N-(1,3,4,6-tetra-0-benzyl-beta-D-glucopyranose-2-group)-2-amido-4-substituted thiazole is synthesized by cyclizing the novel intermediate glycosyl thiourea-N-(1,3,4,6-tetra-0-benzyl-2-deoxygenation beta-D-glucopyranose-2-group) thiocarbamide with 1-bromine-2-substituted ethyl ketone. The synthetic method disclosed by the invention has the advantages of easiness and safety for operation, wide application scope, low cost of the raw material, easiness for raw material obtaining, easiness and convenience for post-processing and high yield, is a fast high-efficiency synthetic method and has a wide application prospect on the aspect of preparing an acetylcholinesterase resistant drug because the synthesized compound has high inhibiting effect on acetylcholinesterase.

The invention discloses an electrochemical biosensor based on polypeptide analog with the electrocatalytic activity for acetylcholin esterase detection. The biosensor for acetylcholin esterase detection is characterized by comprising the following steps: (1) intensively stirring and uniformly mixing silver nitrate, L-cysteine and secondary distilled water on a stirrer, and incubating in an oscillator; (2) electrodepositing graphene onto a bare glassy carbon electrode by utilizing an electrochemical method, mixing 0.05 weight percent of a Nafion solution with the solution obtained in the step (1), and dropwise coating GO / GCE to obtain an electrochemical biosensor based on the polypeptide analog with electrocatalytic activity (CP / GO / GCE); (3) performing acetylcholin esterase activity detection, namely catalyzing acetylcholine in one step to generate H2O2, uniformly mixing acetylcholine, AchE and choline oxidase, and incubating to generate H2O2, and detecting the solution with the sensorprepared in the step (2) with good specificity, high sensitivity, high detection speed, accurate and reliable result; and (4) performing AChE inhibitor malathion detection, namely uniformly mixing acetylcholine, AChE, malathion and choline oxidase, incubating, and detecting the solution with the sensor prepared in the step (2). The biosensor has the advantages of good specificity, high sensitivity, high detection speed and accurate and reliable result.

The present invention relates to novel analogs of choline and methods of use or treatment of neurodegenerative disorders and / or conditions such as Parkinson's disease, Huntington disease, Alzheimer's disease and related disorders such as amyotrophic lateral sclerosis, spinal muscular atrophy, Friedrich's ataxia, Pick's disease, Bassen-Kornzweig syndrome, Refsom's disease, retinal degeneration, Cruetzfelt-Jacob syndrome or prion disease (mad cow disease), dementia with Lewy bodies, schizophrenia, paraneoplastic cerebellar degeneration and neurodegenerative conditions caused by stroke. The present compounds are effective to treat any neurological condition where acetylcholine transmission neurons and their target cells are affected. Compounds according to the present invention are effective to alleviate and / or reverse the effects of a neurodegenerative condition, prevent further deterioration and / or enhance cognition and memory in patients suffering from neurodegenerative disorders, especially Alzheimer's disease.

The invention provides a 1,3,4-oxadiazole derivative containing glucosamine molecules. R in the molecular formula is selected from -CH3, -C2H5, -C4H11, -C6H5, -C5H4N, 4-OHC6H4-, 3-CH3C6H4, 4-CH3C6H4-, 3-CH3OC6H4-, 2-NO2C6H4-, 4-NO2C6H4-, 2-FC6H4-, 4-FC6H4-, 2-ClC6H4-, 3-ClC6H4-, 3-BrC6H4-, 4-IC6H4-, 4-N (CH3)2C6H4-, -C4H3S, and -C4H3O. The invention further discloses a synthesis method and application of the 1,3,4-oxadiazole derivative containing the glucosamine molecules. The adopted synthesis method has the advantages of being easy and convenient to implement, rapid, high in applicability, efficient and the like. An adopted cyclization reagent has the advantages of being low in price, low in toxicity and the like. The glucosamine molecules are introduced into the 1,3,4-oxadiazole molecules, and the synthesized target compound has the strong inhibitory activity for acetylcholin esterase, thereby having the wide application prospect in the aspect of preparation of anticholinesterase medicine.

The invention provides a multi-target tacrine derivative and a preparation method and application thereof. The compound has the structure shown in a formula (I) (please see the specification in the formula (I)), wherein n is a natural number greater than 1; m is 1 or 2; p is 0 or 1; R1 is H or halogen; and Y is selected from the following structures (please see the specification for the structures) or, a pharmaceutically acceptable salt of a compound in a formula (X). According to the compound, activity of histone deacetylase (HDACs), acetylcholinesterase (AChE) or butyryl cholinesterase (BChE) can be effectively inhibited.

The invention relates to novel hybrid cholinesterase inhibitors containing the melatonin or its oxidation products unit and tetrahydroacridine unit linked via a carbamate bond. Due to the high selectivity of action, which is expressed with high ratio of IC50 for acetylcholinesterase inhibition to IC50 for butyrylcholinesterase inhibition ([IC50(AChE)] / [IC50(BChE)]), the novel compounds may be used in relief and / or treatment of the neurodegenerative diseases, among them the Alzheimer's disease.

The invention relates to a 4-cyclamine alkoxy-3-methoxyl cinnamic acid benzamide compound, a preparation method and application of the compound.The chemical structure general formula of the compound is shown as a formula (I) (please see the formula in the description), wherein m represents 1-12, R1 represents C1-C12 alkyl or benzyl or substituted benzyl, X represents C1-C12 alkyl or benzyl or substituted benzyl, phenyl of which a benzene ring is substituted with 1-4 groups selected from F, Cl, Br, I, C(1-4) alkyl, C(1-4) alkoxy, trifluoromethyl, trifluoromethoxy, nitryl, a cyano group, and the substituent groups can be located at any position of the benzene ring.Accordingly, the compound has the significant inhibiting effect on acetylcholinesterase, the IC50 of the compound is 0.01-5 micromoles per liter, the inhibiting activity on acetylcholinesterase is greatly higher than the inhibiting activity on butyrylcholine esterase, and therefore the compound has the certain selective inhibiting effect on acetylcholinesterase.

The invention discloses a method for determining organophosphorus, particularly relates to a method for determining organophosphorus pesticide by double-enzyme mediated Fe<3+> quenching up-conversionfluorescence, and belongs to the technical field of fluorescence analysis. The specific detection method comprises the following steps: uniformly mixing an organophosphorus solution and an acetylcholine esterase (AChE) solution, incubating, adding the acetylcholine (ACh) solution and a choline oxidase (ChOx) solution to obtain an enzyme reaction solution, sequentially adding the enzyme reaction solution and a bivalent iron salt solution into a PAA-UCNPs nano material solution, reacting in a dark place, determining the fluorescence intensity of the reaction solution after the reaction is finished, and determining the organophosphorus content according to the fluorescence intensity. The method is sensitive, reliable and accurate, and has a great application prospect in the field of food safety organophosphorus pesticide detection.

The invention discloses a fluorescent probe for determining acetylcholin esterase as well as a preparation method and application of the fluorescent probe. The fluorescent probe is an HBTP-Cu<2+> compound, and the HBTP-Cu<2+> compound disclosed by the invention takes competitive combination of HBTP and thiocholine (generated by specifically catalyzing acetylcholine through acetylcholin esterase) and Cu<2+> as a basic action mechanism, and takes HBTP luminescence as a fluorescent signal reporting group. When acetylcholin esterase is added into the system, thiocholine can be coordinated and combined with Cu<2+> in the HBTP-Cu<2+> compound structure, the compound structure is disintegrated, the existing form of HBTP is converted from ordered aggregation to monomer dispersion in a bulk phase solution, and color and fluorescence change of the solution is generated. The recognition effect of the HBTP-Cu<2+> compound on acetylcholin esterase is studied through a nuclear magnetic resonance method, an ultraviolet-visible absorption spectrum method and a fluorescence spectrum method, results show that the HBTP-Cu<2+> compound can efficiently and selectively recognize acetylcholin esterase ina pure water solvent system, and the HBTP-Cu<2+> compound has very high sensitivity on acetylcholin esterase.

The invention relates to the technical field of battery recycling, and particularly relates to a process for recycling waste lithium batteries. The method comprises the steps of preparing a deep eutectic solvent, specifically, mixing and heating quaternary ammonium salt and an amine compound to obtain the deep eutectic solvent, wherein the quaternary ammonium salt is selected from one or a mixtureof more of choline chloride, choline acetate and acetylcholine, and the amine compound is selected from one or a mixture of more of urea, ethanolamine and triethanolamine; and leaching, specifically,leaching the lithium cobalt oxide positive plate by using the eutectic solvent, and disassembling the positive plate from the waste lithium battery to obtain a metal-containing solution and an aluminum foil current collector. The method has the advantages of wide raw material source, low cost, simple process steps and the like.

The invention belongs to the technical field of medicines, in particular to a 3, 4-dihydro-2(1H)-quinolinone-7-O-alkyl-1, 2, 3, 4-tetrahydroisoquinoline compound and a preparation method and application thereof. The compound has the chemical structural general formula shown as a formula I. The compound has an inhibitory effect on butyrylcholinesterase, acetylcholinesterase, MAO-A and MAO-B, and has a selective inhibitory effect on butyrylcholinesterase, is a multi-target inhibitor, and further shows a better therapeutic effect on Alzheimer's disease in an in-vivo experiment, has low toxicity, and has a very good clinical application prospect.

The invention relates to carboline compounds, and a synthetic method and anti-acetylcholinesterase activity thereof. The carboline compounds have been proved to have a plurality of biological activities like anti-microbe activity, anticancer and anti-virus activity; however, researches on the anti-acetylcholinesterase activity and anti-cholinesterase activity have not been reported in the prior art. The carboline compounds provided by the invention are classified into a class A, a class B, a class C and a class D, wherein the class B and the class D are 2-aryl carboline salt compounds while the class A and the class C are 2-aryl-3,4-dihydrocarboline salt compounds. Results of activity determination show that the compounds provided by the invention have substantial anti-acetylcholinesterase and anti-cholinesterase activities and have great potential as active ingredients for preparation of drugs used for treating the Alzheimer disease.

Disclosed herein are methods for the large-scale production of a highly thermally stable acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Additionally, the expression methods disclosed herein can produce ChE preparations consisting of extract or purified forms that can be produced in high amounts and are highly thermally stable. These ChE products can be used in vitro detection, detoxification and decontamination methods.

The invention provides a biological sensor, a preparation method, application in detection of acetylcholin esterase, and a method for detecting an organophosphorus pesticide. The method is characterized in that acetyl choline has mimic enzyme to catalyze and oxidize TMB (3,3',5,5'-tetramethylbenzidine) to discolor, and can be decomposed and deactivated by the acetylcholin esterase, but the decomposing of hydrogen peroxide cannot be catalyzed, so that the TMB solution cannot generate color developing reaction; a linear relationship of absorbance and acetylcholin esterase is built, and the acetylcholin esterase is quantitatively detected; furthermore, the organophosphorus pesticide can effectively inhibit the activity of the acetylcholin esterase, the acetylcholin esterase is deactivated, the deactivated acetylcholin esterase cannot decompose the acetyl choline, and the TMB solution is promoted to generate the color developing reaction, thereby detecting the organophosphorus pesticide.

The invention provides a method for the diagnosis of dementia and transmissible spongiform encephalopathies in a subject by detecting the levels of glycoproteins that bind wheat germ agglutinin. The invention also provides a method for diagnosis of dementia and transmissible spongiform encephalopathies in a subject by comparing the levels of glycoproteins that bind wheat germ agglutinin with the glycosylation patterns of biomarkers, acetylcholinesterase and butyrylcholinesterase.

The invention provides acetylthiocholine iodide and a preparation method thereof. The preparation method comprises the following steps: step S1, dissolving a dimethylamino ethane compound in alkali toobtain an alkaline aqueous solution; step S2, adding a thioacetic acid compound into the alkaline aqueous solution to subject the thioacetic acid compound and the dimethylamino ethane compound to a thioreaction to generate (2-(dimethylamino)ethyl)ethanethioate; S3, separating the (2-(dimethylamino)ethyl)ethanethioate, and preparing an organic solution of the (2-(dimethylamino)ethyl)ethanethioate;and S4, dropwise adding an organic iodide into the organic solution of the (2-(dimethylamino)ethyl)ethanethioate for an iodination reaction so as to generate the acetylthiocholine iodide. According to the preparation method provided by the embodiment of the invention, the high-purity acetylthiocholine iodide can be obtained, and the titration content, the melting point, the clarity and other dataof the product are very excellent. The method is simple in operation, good in safety, suitable for industrial production, easy in raw material obtaining and low in cost.

The invention relates to the technical field of electrochemical analysis, in particular to an AchE / GQDs / N-F-TiO2Ns photoelectrochemical biosensor preparation method and application thereof. The AchE / GQDs / N-F-TiO2Ns photoelectrochemical biosensor comprises GQDs / N-F-TiO2Ns and AchE, wherein the GQDs / N-F-TiO2Ns comprises a nitrogen and fluorine-doped titanium dioxide nanotube array (N-F-TiO2Ns) and graphene quantum dots (GQDs) modified on the titanium dioxide nanotube array; cross-linking modification of acetyl cholinesterase (AchE) on the GQDs / N-F-TiO2Ns is carried out; the wavelength at the highest photocurrent response of the photoelectrochemical biosensor is 365 nm; and cross-linking modification of the AchE on the GQDs / N-F-TiO2Ns is carried out through chitosan. The modification of the graphene quantum dots improves the photocurrent intensity. Due to the good biocompatibility, the immobilization of the enzyme on an electrode is improved, and the affinity of the enzyme to a substrateis improved at the same time. the method can also be used in detection study related to acetylcholine and acetyl cholinesterase.

The element is a structural body of fixed acetylcholinesterase in semisolid state injected into a tubular body with tubules at two ends and enzyme blocking layer is set at bottom of tubular body to prevent fixed enzyme from running off as fixed enzyme is acetycholinesterase using gel as carrier in semisolid state. The method is preprocessing gel first, then coupling ligand of procainamidium for fixing enzyme of acethycholinesterase, finally adding accurately prepared and fixed acethycholinesterase with activity of 1-5 U to a tubular column with tubules at two ends and a bolting-silk round slice placed in flat at bottom.

The invention discloses gel for detecting the content of organic phosphorus and carbamate pesticide. The gel is prepared from polyvinyl alcohol, a chitosan acetic acid solution, formaldehyde having amass concentration of 37 percent, a cholinesterase solution having the vitality of 0.2U / ml and a color developing agent. According to the gel, nerve conduction metabolite acetyl choline is hydrolyzedby catalysis of acetylcholin esterase, the hydrolysate reacts with the color developing agent DTNB to generate a yellow substance, the absorbance is detected to calculate the inhibition rate along with time change, whether the organic phosphorus or carbamate pesticide has the high dosage principle or not is determined, and enzyme-containing gel is prepared from polyvinyl alcohol (PVA), acetic acid, chitosan (CS), formaldehyde, acetylcholin esterase and DTNB, so that the gel has strong water absorbability; and the gel contains acetylcholine esterase and the color developing agent, can be directly used for detecting the content of organic phosphorus and carbamate pesticide along with simple operation steps and less time; and furthermore, the gel serving as an enzyme carrier can be stored ina refrigerator conveniently to ensure the enzyme activity, so that the detection accuracy is improved.

Described is the efficient synthesis of an easy to manipulate and utilize, soluble tartrate salt of a potent, reversible butyrylcholinesterase inhibitor, (−)-(3aS)-3a-methyl-1,2,3,3a,8,8a-hexahydropyrrolo-[2,3-b]indol-5-yl N—4′-isopropylphenylcarbamate (“MHI tartrate”), for use in altering the enzymatic activity of butyrylcholinesterase and / or acetylcholinesterase in a subject exhibiting or predicted to exhibit cognitive disorders associated with diabetes. Subjects may be suffering from or predicted to suffer from abnormal acetylcholinesterase and / or butyrylcholinesterase activity levels or from an inability to metabolize or catabolize blood sugar normally. The method comprises administering to the subject an effective amount of MHI tartrate dispensable in discrete pharmaceutically useful dosages. MHI tartrate effectively inhibits both acetylcholinesterase and butyrylcholinesterases and additionally is highly selective for butyrylcholinesterase over acetylcholinesterase.

The invention relates to the fields of nanometer catalysis, environmental protection and analytical chemistry, and specifically includes a test strip for rapidly detecting pesticides. In-situ generation of iron ferric oxide nanoparticles with imitation peroxidase activity on test strips and modification of acetylcholinesterase and choline oxidase, when organophosphorus pesticides exist, it will irreversibly inhibit the activity of acetylcholinesterase , so that the amount of choline generated by the hydrolysis of acetylcholine is reduced, resulting in the reduction of hydrogen peroxide generated through the cascade reaction, and the color development of the chromogenic substrate becomes lighter. Moreover, the chromatographic phenomenon of the test paper can eliminate the interference of other substances in the sample. The convenient and quantitative detection of acetylcholine can be realized through mobile phone camera and mobile phone software analysis. The invention can be used in the fields of pesticide residue analysis, biomedicine, environmental monitoring and the like.

The invention discloses a gel for detecting content of organophosphorus and carbamate pesticide. The gel is prepared from polyvinyl alcohol, a chitosan acetic acid solution, formaldehyde with a mass concentration of 37 percent, and a cholinesterase solution with vitality of 0.2U / ml. Cccording to the principle that cholinesterase is used to catalyze the hydrolysis of indigo phenol acetate into acetic acid and indigo phenol, and the organophosphorus or carbamate pesticide can inhibit choline ester, enzyme-containing gel is prepared from polyvinyl alcohol (PVA), acetic acid, chitosan (CS), formaldehyde and acetylcholinesterase, so that the gel has very high water absorption. The gel contains acetylcholinase, so that the gel can be directly used for the detection of the content of organic phosphorus and carbamate agricultural pesticide. Moreover, the gel is used as an enzyme carrier, and is easy to store in a refrigerated container in order to ensure the enzyme activity and improve the accuracy of detection.