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Identification element of organic phosphorus pesticide and its making method

A technology for organophosphorus pesticides and identification components, which is applied to measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems of inconvenient operation, poor repeatability, difficulty in achieving stability, firmness and uniformity, etc., and achieve long working life, Good linearity and high sensitivity

Inactive Publication Date: 2009-05-06
OCEAN UNIV OF CHINA
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Problems solved by technology

However, experiments have confirmed that the immobilized enzymes formed by this technique are difficult to achieve stability, firmness and uniformity because they are immobilized on the cellulose membrane. They are usually suitable for one-time use, and the operation is inconvenient and the repeatability is poor.

Method used

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  • Identification element of organic phosphorus pesticide and its making method
  • Identification element of organic phosphorus pesticide and its making method

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Embodiment Construction

[0011] like figure 1 , the present invention is a structure in which semi-solid immobilized acetylcholinesterase 2 is injected into a tubular body 1 with thin tubes 4 at both ends. The immobilized enzyme 2 as the sensitive material is acetylcholinesterase with gel as the carrier, which is in a semi-solid state. The acetylcholinesterase is suitable for using the acetylcholinesterase extracted and purified from animals such as fish, flies, electric eels, insects and the like. The added amount (expressed as enzyme activity) depends on the concentration of organophosphorus pesticides in the water sample to be tested. In consideration of preventing the loss of the enzyme, an enzyme blocking layer 3 for preventing the loss of the immobilized enzyme 2 can also be provided at the lower part of the tubular body 1 . The enzyme-inhibiting layer 3 should preferably be made of 300-400 mesh sieve silk or glass fiber. The function of the thin tubes 4 at both ends of the tubular body 1 is ...

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Abstract

The element is a structural body of fixed acetylcholinesterase in semisolid state injected into a tubular body with tubules at two ends and enzyme blocking layer is set at bottom of tubular body to prevent fixed enzyme from running off as fixed enzyme is acetycholinesterase using gel as carrier in semisolid state. The method is preprocessing gel first, then coupling ligand of procainamidium for fixing enzyme of acethycholinesterase, finally adding accurately prepared and fixed acethycholinesterase with activity of 1-5 U to a tubular column with tubules at two ends and a bolting-silk round slice placed in flat at bottom.

Description

technical field [0001] The invention relates to an organophosphorus pesticide identification element and a preparation method thereof. Background technique [0002] In recent years, enzyme sensors have received more and more attention in the monitoring of environmental pollutants due to their advantages of rapidity, sensitivity, and no secondary pollution. Enzyme sensors are mainly composed of recognition elements and transducers. The latter is generally a pH electrode, and its production technology is relatively mature; while the former is an immobilized sensitive biological enzyme, and there is no ideal method in the preparation technology. If the sensitive biological enzyme cannot be firmly bound to the carrier to form a sensitive and stable recognition element, the enzyme sensor composed of it cannot continuously and automatically detect the target pollutants in the environment. [0003] At present, the common method of acetylcholinesterase immobilization is to use glu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30
Inventor 孟范平唐学玺朱小山何东海马冬杨正先
Owner OCEAN UNIV OF CHINA
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