Preparation for detecting organophosphorus pesticide residual test paper and its use method

An organophosphorus pesticide and test paper technology, which is applied in the field of analytical chemistry, can solve the problems of inconvenient portability of instruments, cumbersome pretreatment operations, and difficulty in adapting to rapid and large-scale residual analysis of samples. Simple preparation method

Inactive Publication Date: 2008-11-05
GUANGXI ACAD OF SCI
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Pesticide residue analysis traditionally mainly uses gas chromatography, gas chromatography-mass spectrometry, and high-performance liquid chromatography, which can measure the residues more accurately, but requires large-scale instruments, cumbersome pre-treatment operations, and specially trained personnel. In addition, the instrument is not convenient to carry and move, and it is difficult to adapt to the requirements of simple and fast on-site and large-scale sample residue analysis

Method used

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Examples

Experimental program
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Embodiment Construction

[0012] Implementation column 1:

[0013] Prepare 2.0 μg / mL dichlorvos with distilled water, take two 1.5 mL eppdof centrifuge tubes, add 500 μL 2.0 μg / mL dichlorvos and 500 μL 0.5% bromine water to one tube and let stand for 15 minutes, add 500 μL distilled water to the other tube and 500 μL of 0.5% bromine water to stand for 15 minutes, take 2 pieces of acetylcholinesterase enzyme tablets, add 40 μL each of the above bromine water-treated dichlorvos pesticide solution and the control, and let stand for 10 minutes; then add 60 μL of acetylcholine chloride substrate Solution, let it stand for 10min; finally add 50μL of Fast Blue B color developing solution, it is observed that the enzyme tablet added with dichlorvos is almost colorless, while the control enzyme tablet is light red.

[0014] Implementation column 2:

[0015] Prepare 1.5μg / mL methamidophos with distilled water, take 2 1.5mL eppdof centrifuge tubes, add 500μL 1.5μg / mL methamidophos and 500μL 0.5% bromine water to...

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Abstract

The present invention discloses a preparation method of a test paper for testing the residual organphosphorous pesticide and a using method thereof, wherein the preparation method comprises the following procedures: (1) preparing acetylcholine esterase with fresh blood of hen or female duck as raw material; (2) fixing the acetylcholine esterase on the filter paper; (3) slicing when the filter paper is frozen and dried. A substrate liquid, a color developing liquid and a sensitization liquid are provided. The principle is that the inhibition of the organphosphorous pesticide which is processed by the sensitization liquid to the acetylcholine esterase in the chicken or duck blood serum to make that the substrate of naphthyl acetate of the acetylcholine esterase can not be decomposed thereby that the displayed colors are not consistent when the fast blue B is added into to obtain the purpose of determining whether the sample contains the residual organphosphorous pesticide. The method of the invention has the advantages of low cost, simple and fast operation. The method is suitable for determining whether the residual organphosphorous pesticide exists in vegetable, fruit and food on-site.

Description

Technical field: [0001] The invention belongs to the field of analytical chemistry and in particular relates to the preparation and application method of a test paper for detecting organophosphorus pesticide residues. Background technique: [0002] At present, pesticide residues have become a major problem threatening food safety. It is very urgent to increase the detection of pesticide residues and strengthen the monitoring of pesticide residues in foods such as vegetables and fruits on the market. Pesticide residue analysis traditionally mainly uses gas chromatography, gas chromatography-mass spectrometry, and high-performance liquid chromatography, which can measure the residues more accurately, but requires large-scale instruments, cumbersome pre-treatment operations, and specially trained personnel. Moreover, the instrument is inconvenient to carry and move, and it is difficult to adapt to the requirements of simple and rapid on-site and large-scale sample residue anal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78C12Q1/46
Inventor 赵肃清黄洪彭元
Owner GUANGXI ACAD OF SCI
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