83 results about "Acetyl choline" patented technology

The invention discloses a microelectrode array chip for multi-parameter detection of nerve cells and a preparation method thereof, relating to a sensor technology. The chip consists of seven parts including an insulating base, a microelectrode array, counter electrodes, reference electrodes, electrode leads, contacts, a superficial insulating layer and a modification material, wherein the electrodes are prepared by using a micro-electro-mechanical system (MEMS) process, and a specific nano-composite material and enzymes are modified at fixed points on the surfaces of the electrodes. The nerve cells are cultivated on the surfaces of modified working electrodes, and by combining the counter electrodes and the reference electrodes, the microelectrode array chip can be used for detecting electrophysiological signals of the nerve cells and electrochemical signals of neurotransmitters such as dopamine, acetyl choline and the like in real time at the same time, and has a function of applying electrical stimulation to the nerve cells. The microelectrode array chip integrates functions, modifies a material at fixed points, is convenient to use, and is suitable for culturing the nerve cells and making related research on multi-parameter detection of the nerve cells in laboratories.

There is described a method for increasing the maximal tolerated close and thus the efficacy of an acetyl choline esterase inhibitor (AChEI) in a patient suffering from an Alzheimer type dementia by decreasing concomitant adverse effects by administration of said AChEI in combination with a non-anticholinergic antiemetic agent, whereby an enhanced acetyl choline esterase inhibition in the CNS of said patient is achieved and alleviation of the symptoms of Alzheimer type dementia in said patient is thereby improved to a greater extent. The use of a non-anticholinergic antiemetic agent for the preparation of a pharmaceutical composition for the treatment of Alzheimer type dementia in combination with an acetyl choline esterase inhibitor (AChEI) and pharmaceutical compositions comprising (a) a 5HT₃ receptor antagonist, a dopamine antagonist, a H1-receptor antagonist, a cannabinoid agonist, aprepitant or casopitant as an antiemetic agent and (b) an acetylcholine esterase inhibitor are also described.

PCT No. PCT/SE96/01677 Sec. 371 Date Sep. 21, 1998 Sec. 102(e) Date Sep. 21, 1998 PCT Filed Dec. 17, 1996 PCT Pub. No. WO97/22339 PCT Pub. Date Jun. 26, 1997This invention relates to a method for treating snoring, sleep apnea and other form of sleep-disordered breathing, which comprises administration of a therapeutically effective dose of an acetyl choline esterase inhibitor (CEI) such as pyridostigmine or a pharmaceutically acceptable salt thereof.

The invention discloses a device and a method for detecting pesticide residue based on a multilayer paper microfluidic chip in the technical fields of microfluidic technology and pesticide residue. The method comprises the following steps: adding a to-be-detected sample into a cutting and stirring device, cutting and stirring to obtain sample mixed liquid, adding distilled water into a water-absorbing pad at the left sampling opening dropwise, enabling the liquid to arrive at a lime heating area through a paper channel, heating a paper chip in the lime heating area, adding the sample mixed liquid into the water-absorbing pad at the left sampling opening dropwise, combining the sample mixed liquid and an acetyl choline fixture after passing through a hydrophobic channel, producing an inhibition reaction on acetylcholin esterase through a left paper channel, developing, detecting a developing solution by using a photoelectric detection device, and processing a detection signal by a controller to obtain the concentration of pesticide. According to the method and the device, sample introduction, heating, reaction and developing are integrated, the detection cost is low, the detection speed is high and the accuracy is high.

The invention discloses a polypeptide combination method for wrinkle reduction. Through one or more polypeptides, the fusion of vesicles and a cell membrane is inhibited so that release of acetyl choline is inhibited. The polypeptide combination method utilizes a wrinkle reduction polypeptide composition with a mass concentration of 0.001% to 5%, a humectant with a mass concentration of 0.001%-10%, an antibacterial stabilizer with a mass concentration of 0.001%-2%, a preservative with a mass concentration of 0.001%-2% and a pH adjuster. The wrinkle reduction polypeptide composition comprises one or more of acetyl hexapeptide-8, palmitoyl pentapeptide-4, venom-like peptide and copper tripeptide-1 and the balance of deionized water and has a mass concentration of 0.001%-5%. According to thewrinkle production mechanism and different polypeptide action targets, the invention provides the wrinkle reduction polypeptide composition.

The invention provides a preparation method and application of a cobaltosic oxide/graphene aerogel composite material. The cobaltosic oxide/graphene aerogel composite material can be used in the field of colorimetric method based detection, especially used for colorimetric method based acetyl choline detection. A water solution prepared by oxidized graphene and soluble cobalt salt is transferred into a cylindrical container, then the cylindrical container is moved into a polytetrafluoroethylene hydrothermal kettle to perform constant-temperature thermal reaction, a drying technology is adopted to prepare the cobaltosic oxide/graphene aerogel composite material, and the preparation method is simple. The prepared cobaltosic oxide/graphene aerogel composite material has excellent conductivity, good electronic transmission performance and large localization range, and catalytic effect is remarkably improved. The prepared cobaltosic oxide/graphene aerogel composite material is creatively applied to colorimetric method based acetyl choline detection, operation is simple and convenient, the used instruments are low in price, and the research field of graphene aerogel based materials is widened.

The invention discloses a method for detecting the concentration of acetylcholin esterase. The method comprises the following steps that: S1: preparing carbon quantum dots, and preparing the carbon quantum dots into carbon quantum dot solution; S2: preparing acetylcholin esterase standard solutions of different concentrations, and independently adding acetyl choline solution, choline oxidase solution, horseradish peroxidase solution and o-phenylenediamine solution into the carbon quantum dot solution, and obtaining a mixed liquid system; S3: independently adding the carbon quantum dot solutioninto the mixed liquid system to obtain a standard sample system; S4: independently detecting the fluorescence intensities I442 and I573 of the standard sample system on the positions of 442nm and 573nm, and establishing a linear relational expression between I573/I442 and the concentration of the acetylcholin esterase; and S5: preparing the to-be-detected solution of the acetylcholin esterase, and referring to S2, S3 and S4 to obtain the concentration of the acetylcholin esterase in the to-be-detected solution of the acetylcholin esterase. The method has the advantages of high-sensitivity identification and the like of the acetylcholin esterase in the sample.

The invention relates to a medical composition having the effect of restraining acetyl cholinesterase, as well as a synthetic method and an application of the medical composition, in particular to a carbamate compound with a general formula (I), wherein the definition of a symbol A is as shown in the description. The invention discloses the structure of the carbamate compound, the synthetic method thereof and the application of the carbamate compound in restraining the in vitro activity of acetyl cholinesterase, and an insecticide and a new medicine for treating Alzheimer's diseases can be further developed from the carbamate compound.

Compounds represented by general formula (I), their stereoisomers, tautomers, derivatives, prodrugs or pharmaceutically acceptable salts, and their preparation methods or uses for the manufacture of a medicament of analgesics. In which R₁ is selected from H, substituted or unsubstituted phenyl, or substituted or unsubstituted heteroaryl; A is bond, or saturated or unsaturated straight-chain or branched-chain hydrocarbon radical; R₂, R₃ are each independently hydrogen or methyl, which linked with any position of spirocyclo-structure; n and m are each independently integer between 0-2, do not represent 0 at the same time; B and D are each independently C₁-C₃ straight-chain or branched-chain alkylene; Y is selected from —CHR₄—, O, S, —S(O)—, —SO₂—, —NR₄— and substituted or unsubstituted phenylene, in which R₄ represents H, C₁-C₆ saturated or unsaturated alkyl, methyl or ethyl substituted by substituted or unsubstituted aryl or heteroaryl; and X⁻ is pharmaceutical acceptable organic or inorganic anion. These compounds can be used as muscarine receptor (M-receptor) and/or nicotine acetylcholine receptor (N-receptor) agonist or antagonist. These compounds have good analgesic effect without side effect such as addiction.

The present invention relates to novel heteroaryl diazacycloalkane derivatives which are found to be cholinergic ligands at the nicotinic Acetyl Choline Receptors. Due to their pharmacological profile the compounds of the invention may be useful for the treatment of diseases or disorders as diverse as those related to the cholinergic system of the central nervous system (CNS), diseases or disorders related to smooth muscle contraction, endocrine diseases or disorders, diseases or disorders related to neurodegeneration, diseases or disorders related to inflammation, pain, and withdrawal symptoms caused by the termination of abuse of chemical substances.

The invention provides a method for detecting the acetyl choline content in peripheral blood mononuclear cells. The method provided by the invention lays a foundation for accurately detecting the ACh (acetyl choline) content by separating an ACh ingredient from a sample to be detected by adopting a UPLC (ultra performance liquid chromatography) technology. According to the technical scheme, an HILIC (hydrophilic interaction chromatography) column is found to be more suitable for separation of a polar compound by adopting experimental measures, can be used for effectively separating ACh from iso-ACh, and has a better separation effect on Cho. On this basis, chromatographic conditions and internal standard substance categories are further designed, and d9-ACh of isotope is taken as national standard substances of ACh and iso-ACh at the same time to furthest reduce the analysis error caused by sample damage and effectively ensure the accuracy of a measurement result. According to the method provided by the invention, on the basis of effectively separating ACh by using the UPLC, ACh signal detection is performed by a liquid chromatography-mass spectrometry system, and the ACh is accurately quantified by optimizing mass spectrometer conditions with a stable and accurate result and favorable linearity and sensitivity; meanwhile, the method has relatively low detection limit and relatively high recovery rate and also has a broad application prospect.

The invention provides a detection method for obtaining rat sinkaline signals through Proton MR spectroscopy (<1>H MRS) and then indirectly reflecting the content of acetylcholines in a rat. The method compares the content relationship of total sinkaline and acetylcholines in a sea horse area, a striaturn area, a frontal cortex area and a somatic cortex area of the normal rat; the contents of the total sinkaline and the acetylcholines of the rat in the four brain areas have obvious relativity through double variant and linear regression analysis. As shown by the result, the rat sinkaline signals obtained through the Proton MR spectroscopy are used as the predicated value of the content of the acetylcholines in the rat, which has statistic significance. Simultaneously, loss of the acetylcholines is a very important neuropathological variation of presenile dementia, thereby the method is hopeful to be applied to noninvasive early clinical diagnosis of the presenile dementia.

The invention discloses application of berberine or other pharmaceutically acceptable salts in preparing a M3 receptor selective inhibitor medicine. The medicine is used for treating acute pancreatitis, lung cancers and colon cancers, self-immunity sialadenitis, asthma, respiratory tract inflammation, lung diseases and cardiovascular diseases, relieving panic, or treating excessive activity of bladders, and is used for inhibiting the enhancing of Ca<2+> signals in pancreatic acinar cells caused by M3 receptor-mediated acetyl choline and L-arginine.

The invention discloses an application of pentapeptide KLPGF in inhibiting AChE activity. The amino acid sequence of AChE inhibiting peptide is Lys-Leu-Ro-Gly-Phe (KLPGF). The KLPGF activity peptide can effectively inhibit the AChE activity, improve the level of brain neurotransmitter acetyl choline and play the effect of preventing senile dementia. The AChE inhibiting peptide is simple in structure, safe and free from toxicity; the AChE inhibiting property is strong and can be used for developing healthcare food or drug for assisting to release or treat the senile dementia.

The invention provides a biological sensor, a preparation method, application in detection of acetylcholin esterase, and a method for detecting an organophosphorus pesticide. The method is characterized in that acetyl choline has mimic enzyme to catalyze and oxidize TMB (3,3',5,5'-tetramethylbenzidine) to discolor, and can be decomposed and deactivated by the acetylcholin esterase, but the decomposing of hydrogen peroxide cannot be catalyzed, so that the TMB solution cannot generate color developing reaction; a linear relationship of absorbance and acetylcholin esterase is built, and the acetylcholin esterase is quantitatively detected; furthermore, the organophosphorus pesticide can effectively inhibit the activity of the acetylcholin esterase, the acetylcholin esterase is deactivated, the deactivated acetylcholin esterase cannot decompose the acetyl choline, and the TMB solution is promoted to generate the color developing reaction, thereby detecting the organophosphorus pesticide.

The invention provides application of acetyl choline in improving accumulation of microalgae polyunsaturated fatty acids and alpha-linolenic acid. The invention discloses application of acetyl choline in improving accumulation of microalgae polyunsaturated fatty acids and alpha-linolenic acid, and belongs to the technical field of microalgae organisms. The application comprises the following specific operation steps: (1) adding neurotransmitter acetyl choline in different concentrations at different growth periods of the microalgae; and (2) analyzing influences of the neurotransmitter acetyl choline in different concentrations, which are added at different periods, on polyunsaturated fatty acids and alpha-linolenic acid in microalgae grease. The invention discloses a method of increasing the content of the polyunsaturated fatty acids and alpha-linolenic acid in the microalgae grease in a growth process of the microalgae for the first time, so that a new thought is provided for promoting the accumulation of microalgae polyunsaturated fatty acids and alpha-linolenic acid in the microalgae grease by using a chemical method.