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Application of acetyl choline in improving accumulation of microalgae polyunsaturated fatty acids and alpha-linolenic acid

A technology of acetylcholine and linolenic acid, which is applied in the application field of acetylcholine in increasing the accumulation of polyunsaturated fatty acids and α-linolenic acid in microalgae, can solve the problems of shortage of supply and competition for land, and achieve the effect of promoting the accumulation of polyunsaturated fatty acids

Inactive Publication Date: 2014-12-24
南京以标能源有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the patents on the production of α-linolenic acid mainly focus on the use of various terrestrial plants as raw materials, and the patents on the production of DHA and EPA mainly focus on the production of fish oil, and these traditional substances are used as raw materials for PUFAS It will cause problems such as competition for land and shortage of supply

Method used

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  • Application of acetyl choline in improving accumulation of microalgae polyunsaturated fatty acids and alpha-linolenic acid
  • Application of acetyl choline in improving accumulation of microalgae polyunsaturated fatty acids and alpha-linolenic acid
  • Application of acetyl choline in improving accumulation of microalgae polyunsaturated fatty acids and alpha-linolenic acid

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Experimental program
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Effect test

Embodiment 1

[0047] Preparation of specific medium. In order to investigate the effect of neurotransmitter acetylcholine on polyunsaturated fatty acids in chlorella oil, especially α-linolenic acid, we configured specific medium by adding different concentrations of acetylcholine to basal Tap medium. The concentration of acetylcholine in the control medium was 0μg / L, 0.125μg / L, 0.25μg / L, 0.5μg / L, 1μg / L, 5μg / L, 10μg / L.

[0048] Inoculate fresh Chlorella (Chlorella is self-screened by the laboratory) with an inoculum amount of 5% volume fraction in each of the above-mentioned culture solutions, and cultivate it in a constant temperature light incubator at 25°C and 3000 lux until the stable period. Obtain algae. During the cultivation process, the algae liquid was taken every 24 hours, and the absorbance of the algae liquid was measured at 680 nm to obtain the growth curve of Chlorella.

[0049] The above-mentioned algae liquid is centrifugally concentrated, washed with deionized water, and ...

Embodiment 2

[0054] Tested in a similar manner to Example 1, except that the neurotransmitter acetylcholine was not added at the initial stage of cultivation, and the concentrations of 0 μg / L, 0.125 μg / L, 0.25 μg / L, 0.5 μg / Except for acetylcholine of L, 1 μg / L, 5 μg / L, and 10 μg / L, other parameters remained unchanged.

[0055] Table 2 shows the effects of different concentrations of acetylcholine on the fatty acid composition of Chlorella's growth log phase. The results show that compared with the control group (the concentration of acetylcholine is 0 μg / L), adding acetylcholine in the logarithmic phase of Chlorella growth can increase the content of polyunsaturated fatty acids, especially α-linolenic acid in Chlorella oil to a certain extent , but the increase was slightly lower than that of adding different concentrations of acetylcholine during the adjustment period of Chlorella growth. With the increase of acetylcholine concentration, the content of polyunsaturated fatty acids, espec...

Embodiment 3

[0060] The test was carried out in a similar manner to Example 1, except that the neurotransmitter acetylcholine was not added at the initial stage of culture, and the concentrations of 0 μg / L, 0.125 μg / L, 0.25 μg / L, and 0.5 μg / L were added after culturing to the stationary phase of microalgae growth. , 1 μg / L, 5 μg / L, and 10 μg / L of acetylcholine, other parameters remained unchanged.

[0061] Table 3 The effect of different concentrations of acetylcholine on the fatty acid composition of Chlorella's oil during the stable growth period

[0062]

[0063] Table 3 shows the effects of adding different concentrations of acetylcholine on the fatty acid composition of chlorella during the stable growth period. The results showed that compared with the control group (the concentration of acetylcholine was 0 μg / L), adding acetylcholine in the stable growth period of chlorella could increase the content of polyunsaturated fatty acids, especially α-linolenic acid, in chlorella oil to...

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Abstract

The invention provides application of acetyl choline in improving accumulation of microalgae polyunsaturated fatty acids and alpha-linolenic acid. The invention discloses application of acetyl choline in improving accumulation of microalgae polyunsaturated fatty acids and alpha-linolenic acid, and belongs to the technical field of microalgae organisms. The application comprises the following specific operation steps: (1) adding neurotransmitter acetyl choline in different concentrations at different growth periods of the microalgae; and (2) analyzing influences of the neurotransmitter acetyl choline in different concentrations, which are added at different periods, on polyunsaturated fatty acids and alpha-linolenic acid in microalgae grease. The invention discloses a method of increasing the content of the polyunsaturated fatty acids and alpha-linolenic acid in the microalgae grease in a growth process of the microalgae for the first time, so that a new thought is provided for promoting the accumulation of microalgae polyunsaturated fatty acids and alpha-linolenic acid in the microalgae grease by using a chemical method.

Description

technical field [0001] The invention belongs to the technical field of microalgae biological cultivation, and in particular relates to an application of acetylcholine in increasing the accumulation of microalgae polyunsaturated fatty acids and alpha-linolenic acid. Background technique [0002] Polyunsaturated fatty acids (PUFA S ) refers to straight-chain fatty acids containing two or more double bonds and a carbon chain length of 18-22 carbon atoms, according to PUFA S The first unsaturated bond appears at the methyl end of the carbon chain, which can be mainly divided into three categories: n-3 (or ω-3) PUFA S , represented by DHA (C22: 6n-3), EPA (C20: 5n-3) and α-linolenic acid (C18: 3n-3); n-6 (or ω-6) PUFA S , such as arachidonic acid (C20:4n-6) and γ-linolenic acid (C18:3n-6); the third n-9 class of PUFA S . Among them, omega-3 polyunsaturated fatty acids play a very important role in nutrition and medicine: α-linolenic acid has the functions of regulating blood ...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/89
Inventor 陈以峰阿里.帕萨爱默尔窦晓孙芝兰
Owner 南京以标能源有限责任公司
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