9 results about "Butyrylcholine esterase" patented technology

The invention discloses a lignan compound of which the structure is selected from one of the following structures: the lignan compound prepared by the invention has a remarkable inhibition effect on the activity of butyrylcholine esterase, and the binding free energy of the two lignan compounds and BChE is analyzed by performing molecular docking on the two lignan compounds and the butyrylcholine esterase. A molecular docking result proves a butyrylcholine esterase activity in-vitro inhibition experiment result, so that the lignan compound prepared by the invention has huge potential as a butyrylcholine esterase inhibitor.

The application provides an organic phosphorus poisoning relief composite enzyme and application thereof, and belongs to the technical field of biological medicine. The organic phosphorus poisoning relief composite enzyme provided by the application comprises polyethylene glycol modified organic phosphorus hydrolase and butyrylcholine esterase, and the mass ratio of the polyethylene glycol modified organic phosphorus hydrolase to the butyrylcholine esterase is (4-8):(2500-10000). The rat acute organic phosphorus poisoning treatment result shows that the organic phosphorus poisoning relief composite enzyme provided by the application fully plays the efficient catalytic hydrolysis capacity of the enzyme as a catalyst, efficiently removes organic phosphorus and acetylcholine by using the organic phosphorus hydrolase and the butyrylcholine esterase respectively, provides a drug combination for treating the cause for the organic phosphorus poisoning injury first aid, and the treatment effect is significantly better than that of the single drug.

To provide a component useful as an ATP scavenger and / or an ADP scavenger.SOLUTION: Transferrin is useful as an adenosine triphosphate scavenger, and butyrylcholinesterase is effective as an adenosine diphosphate scavenger.SELECTED DRAWING: None

The invention provides an HSA fluorescent probe as well as synthesis and application thereof. The fluorescent probe is an enzymatic HSA fluorescent probe and has a structure as shown in a formula (I). The fluorescent probe has high selectivity and high sensitivity, can distinguish HSA from other enzymes (such as chymotrypsin, lipase, protease, beta-galactosidase, pepsin, acetylcholin esterase, butyrylcholine esterase, carboxylesterase, trypsin, DNA enzyme, alkaline phosphatase, lysozyme and the like), and has the detection limit as low as 0.27 mu g / mL, so that the fluorescent probe has the advantages of high selectivity, high sensitivity, high sensitivity and the like. The fluorescent probe provided by the invention can realize qualitative and quantitative detection of HSA in serum, urine or cells.

The present invention relates to compositions comprising an antiemetic, particularly including 5-HT3 antagonist and / or NK-1 antagonist antiemetics, in combination with a choline esterase inhibitor such as an acetylcholine esterase inhibitor and / or a butyrylcholine esterase inhibitor, and methods of using the composition to provide improved protection of subjects at risk of organophosphorus poisoning, or, for the treatment, prevention, or alleviation / reduction of toxicity from an organophosphorus compound, by enabling the use of a highly protective daily dose of the choline esterase inhibitor without the typical adverse effects.

The invention provides a pretreatment method and a detection method of a malathion biomarker in blood, and relates to the technical field of analysis and detection. The method comprises the following steps: separating dimethyl phosphoryl butyrylcholine esterase and methyl phosphoryl butyrylcholine esterase by using antibody magnetic beads, and then carrying out enzymolysis by using pepsin to generate dimethyl phosphoryl nonapeptide and methyl phosphoryl nonapeptide, so as to obtain a nonapeptide solution to be detected; carrying out protein precipitation on the blood subjected to IMS treatment, wherein supernate is to-be-detected liquid of malathion and metabolite (malathion dihydroxy acid) of malathion; according to the method, malathion is taken as a template, protein precipitation is digested by streptavidin to generate dimethyl thiophosphoryl tyrosine, to-be-detected dimethyl thiophosphoryl tyrosine solutions are obtained, and the to-be-detected solutions are subjected to subsequent UPLC-MS detection, so that comprehensive monitoring of malathion at different levels and exposure conditions at different time points is realized, the method has a wide application prospect, and the detection method is high in sensitivity and high in precision.

The invention provides a bioluminescent probe for detecting butyrylcholine esterase in a living body as well as a preparation method and application of the bioluminescent probe, and belongs to the field of organic synthesis and detection. In the work, an open-type bioluminescent probe ZD-1 for detecting butyrylcholine esterase with high selectivity and high sensitivity is developed. The probe is manufactured by adding a recognition group of a cyclopropylformyl group to a hydroxyfluorescein skeleton. Cell and in-vivo imaging experiments verify that the probe has the capability of detecting butyrylcholine esterase in a biological system, and can provide a powerful tool for screening butyrylcholine esterase inhibitors. The probe ZD-1 has the advantages of simple preparation method, high yield, low cost, high efficiency, specificity, rapidness, sensitivity and the like, can qualitatively and quantitatively detect butyrylcholine esterase, and is easy to popularize and apply.

The invention relates to a tricyclic thiazolopyrimidine ketone ether derivative and application thereof.The derivative is prepared by the steps that ethyl cyanoacetate serves as an initial raw material, a hydroxylamine compound (A) is generated under the action of sodium nitrite and phosphoric acid, 2-amino ethyl cyanoacetate (B) is obtained through reduction, the 2-amino ethyl cyanoacetate (B) reacts with acetic anhydride and a Lawesson reagent to obtain a 5-amino-4-formate thiazole compound (D), and the 5-amino-4-formate thiazole compound (D) reacts with the sodium nitrite and the phosphoric acid to obtain the tricyclic thiazolopyrimidine ketone ether derivative. The preparation method comprises the following steps: taking 4-bromo-5, 7, 8, 9-tetrahydrothiazole [5 ', 4': 4, 5] pyrimidine [1, 2-a] azepine-11 (5H)-ketone as a raw material, performing NBS bromination to obtain a 2-bromo-dihydropyrrole [1, 2-a] thiazolo [5, 4-d] pyrimidine-9 (5H)-ketone compound (F) and a 2-bromo-6, 7, 8, 9-tetrahydrothiazole [5 ', 4': 4, 5] pyrimidine [1, 2-a] azepine-11 (5H)-ketone derivative (G) under the action of phosphorus oxychloride, and finally reacting with different substituted phenols (thiophenol) under the action of alkali to obtain 40 tricyclic thiazole pyrimidine ketone ether compounds H1-H20 and I1-I20. The invention also investigates the inhibitory activity of the 40 compounds on acetylcholin esterase and butyrylcholin esterase in Alzheimer's disease, and the result shows that 20 compounds have strong inhibitory activity on acetylcholin esterase, and 22 compounds have strong inhibitory activity on butyrylcholin esterase.

The present invention relates to the technical field of butyrylcholine esterase activity detection, and discloses a compound containing an octoate group and applications thereof, the compound has a structure represented by a formula (I), and in the formula (I), a group A is provided by leaving hydrogen on any hydroxyl group from a fluorescent dye molecule containing a hydroxyl group. The novel small-molecule fluorescent probe for detecting the activity of butyrylcholine esterase disclosed by the invention has excellent sensitivity, selectivity and stability, and high market popularization and application values.