Device and method for detecting pesticide residue based on multilayer paper micro-fluidic chip

Abstract

The invention discloses a device and a method for detecting pesticide residue based on a multilayer paper microfluidic chip in the technical fields of microfluidic technology and pesticide residue. The method comprises the following steps: adding a to-be-detected sample into a cutting and stirring device, cutting and stirring to obtain sample mixed liquid, adding distilled water into a water-absorbing pad at the left sampling opening dropwise, enabling the liquid to arrive at a lime heating area through a paper channel, heating a paper chip in the lime heating area, adding the sample mixed liquid into the water-absorbing pad at the left sampling opening dropwise, combining the sample mixed liquid and an acetyl choline fixture after passing through a hydrophobic channel, producing an inhibition reaction on acetylcholin esterase through a left paper channel, developing, detecting a developing solution by using a photoelectric detection device, and processing a detection signal by a controller to obtain the concentration of pesticide. According to the method and the device, sample introduction, heating, reaction and developing are integrated, the detection cost is low, the detection speed is high and the accuracy is high.

Description

technical field [0001] The invention relates to the technical fields of microfluidic technology and pesticide residue detection, and uses a fluidic chip to detect pesticide residues. Background technique [0002] At present, pesticides are still the main method to control crop diseases and insect pests. However, they are highly harmful and difficult to detect residual toxins. Their wide use has also caused a series of food safety and environmental pollution problems. Therefore, how to quickly, efficiently and accurately detect pesticide residues has become an urgent and critical issue. [0003] For the detection of pesticide residues, a one-time quick test card is generally used for detection. Although the cost is low, there is a problem that human pressure is required to fully contact the reactants, which is very easy to cause contamination of the experiment; in addition, due to the optimal temperature of the enzyme used in the detection The temperature is around 37°C, so ...

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the defects existing in the traditional quick test card for the current pesticide residue detection, and proposes an automatic and portable pesticide residue detection device based on multi-layer paper microfluidic chips and a detection method of the device to solve the problem of the existing quick test card The problem of low degree of automation and insufficient detection accuracy enables fast, convenient, automated and accurate detection of pesticide residues

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