Gel for detecting content of organic phosphorus and carbamate pesticide and application thereof

Abstract

The invention discloses gel for detecting the content of organic phosphorus and carbamate pesticide. The gel is prepared from polyvinyl alcohol, a chitosan acetic acid solution, formaldehyde having amass concentration of 37 percent, a cholinesterase solution having the vitality of 0.2U / ml and a color developing agent. According to the gel, nerve conduction metabolite acetyl choline is hydrolyzedby catalysis of acetylcholin esterase, the hydrolysate reacts with the color developing agent DTNB to generate a yellow substance, the absorbance is detected to calculate the inhibition rate along with time change, whether the organic phosphorus or carbamate pesticide has the high dosage principle or not is determined, and enzyme-containing gel is prepared from polyvinyl alcohol (PVA), acetic acid, chitosan (CS), formaldehyde, acetylcholin esterase and DTNB, so that the gel has strong water absorbability; and the gel contains acetylcholine esterase and the color developing agent, can be directly used for detecting the content of organic phosphorus and carbamate pesticide along with simple operation steps and less time; and furthermore, the gel serving as an enzyme carrier can be stored ina refrigerator conveniently to ensure the enzyme activity, so that the detection accuracy is improved.

Description

technical field [0001] The invention relates to a gel for detecting the content of organic phosphorus and carbamate pesticides and an application thereof, belonging to the technical field of agricultural product safety detection. Background technique [0002] In recent years, food safety issues have seriously threatened human health, and the pesticide residues and acute and chronic poisoning in fruits and vegetables caused by excessive use or abuse of pesticides have attracted great attention from the society. At present, there are two main methods for rapid detection of whether the residues of organophosphorus and carbamate pesticides in fruits and vegetables exceed the standard: [0003] 1. Quick test card method, including red tablets (containing indophenol acetate) and white tablets (containing cholinesterase), first drop the solution to be tested on the white tablets (containing cholinesterase), and put Superimpose the white tablet and the red tablet together, and obse...

AI Technical Summary

Problems solved by technology

The detection method is complex to operate and takes a long time period (at least 30min)