Gel for detecting content of organic phosphorus and carbamate pesticide and application thereof

A carbamate and organophosphorus technology, which is used in measurement devices, measurement of color/spectral properties, and analysis by chemical reaction of materials, can solve the problems of complex operation and long time period, and achieve simple operation steps, High accuracy and convenient operation

Inactive Publication Date: 2019-01-11
四川众益生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection method is complex to operate and takes a long time period (at least 30min)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The gel used for the content of organophosphorus and carbamate pesticides provided by this embodiment includes the following components and parts by volume: 100 parts of polyvinyl alcohol, 100 parts of chitosan acetic acid solution, and 2 parts of formaldehyde with a mass concentration of 37%. 10 parts of cholinesterase solution with an enzyme activity of 0.2U / ml, and 10 parts of color developing agent.

[0029] The preparation method of the gel for the content of organophosphorus and carbamate pesticides comprises the steps:

[0030] (1) raw material selection and preparation: choosing polyvinyl alcohol solution, chitosan acetic acid solution, mass concentration is 37% formaldehyde solution and enzyme activity is the cholinesterase solution of 0.2U / ml, described polyvinyl alcohol solution is Prepared by dissolving 1 part by mass of polyvinyl alcohol 1788 in 50 parts by mass of deionized water, the chitosan acetic acid solution is prepared by completely dissolving 1 par...

Embodiment 2

[0035] The gel used for the content of organophosphorus and carbamate pesticides provided by this embodiment includes the following components and parts by volume: 90 parts of polyvinyl alcohol, 90 parts of chitosan acetic acid solution, and 2 parts of formaldehyde with a mass concentration of 37%. , 8 parts of cholinesterase solution with enzyme activity of 0.2U / ml, and 8 parts of chromogen. The manufacturing method of this embodiment is the same as that of Embodiment 1.

Embodiment 3

[0037] The gel used for the content of organophosphorus and carbamate pesticides provided by the present embodiment comprises the following components and parts by volume: 120 parts of polyvinyl alcohol, 120 parts of chitosan acetic acid solution, 2 parts of formaldehyde with a mass concentration of 37% , 20 parts of cholinesterase solution with an enzyme activity of 0.2 U / ml, and 20 parts of color developing agent. The manufacturing method of this embodiment is the same as that of Embodiment 1.

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PUM

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Abstract

The invention discloses gel for detecting the content of organic phosphorus and carbamate pesticide. The gel is prepared from polyvinyl alcohol, a chitosan acetic acid solution, formaldehyde having amass concentration of 37 percent, a cholinesterase solution having the vitality of 0.2U / ml and a color developing agent. According to the gel, nerve conduction metabolite acetyl choline is hydrolyzedby catalysis of acetylcholin esterase, the hydrolysate reacts with the color developing agent DTNB to generate a yellow substance, the absorbance is detected to calculate the inhibition rate along with time change, whether the organic phosphorus or carbamate pesticide has the high dosage principle or not is determined, and enzyme-containing gel is prepared from polyvinyl alcohol (PVA), acetic acid, chitosan (CS), formaldehyde, acetylcholin esterase and DTNB, so that the gel has strong water absorbability; and the gel contains acetylcholine esterase and the color developing agent, can be directly used for detecting the content of organic phosphorus and carbamate pesticide along with simple operation steps and less time; and furthermore, the gel serving as an enzyme carrier can be stored ina refrigerator conveniently to ensure the enzyme activity, so that the detection accuracy is improved.

Description

technical field [0001] The invention relates to a gel for detecting the content of organic phosphorus and carbamate pesticides and an application thereof, belonging to the technical field of agricultural product safety detection. Background technique [0002] In recent years, food safety issues have seriously threatened human health, and the pesticide residues and acute and chronic poisoning in fruits and vegetables caused by excessive use or abuse of pesticides have attracted great attention from the society. At present, there are two main methods for rapid detection of whether the residues of organophosphorus and carbamate pesticides in fruits and vegetables exceed the standard: [0003] 1. Quick test card method, including red tablets (containing indophenol acetate) and white tablets (containing cholinesterase), first drop the solution to be tested on the white tablets (containing cholinesterase), and put Superimpose the white tablet and the red tablet together, and obse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78G01N21/01
CPCG01N21/01G01N21/31G01N21/78
Inventor 张靖新王维静黎威志李方圆张希仁游延军
Owner 四川众益生物科技有限公司
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