AchE/GQDs/N-F-TiO2Ns photoelectrochemical biosensor preparation method and application thereof

A biosensor and photoelectrochemical technology, applied in the field of electrochemical analysis, can solve the problems of high price and achieve the effect of increasing photocurrent, increasing signal and inhibiting recombination rate

Inactive Publication Date: 2019-08-23
YUXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, precious metals have the disadvantages of expensive raw materials, and the ...

Method used

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  • AchE/GQDs/N-F-TiO2Ns photoelectrochemical biosensor preparation method and application thereof
  • AchE/GQDs/N-F-TiO2Ns photoelectrochemical biosensor preparation method and application thereof
  • AchE/GQDs/N-F-TiO2Ns photoelectrochemical biosensor preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Preparation of AchE / GQDs / N-F-TiO 2 Ns photoelectrochemical biosensor

[0041] Step 1: Preparation of N-F-TiO by Electrochemical Anodization Technology 2 Ns electrode

[0042] Before anodizing, first polish the titanium sheet with different sandpaper, then immerse the titanium sheet in distilled water for 10 minutes and ultrasonically clean it with acetone, absolute ethanol, and double distilled water in turn, and ultrasonically clean it for 10 minutes in each substance, and then put the The clean titanium sheet is immersed in the mixed solution of hydrofluoric acid and nitric acid for 1min. The volume ratio of the mixed solution is V(HF):V(HNO 3 ): V(H 2 (0)=1:4:5, and then the titanium sheet was rinsed with acetone and distilled water, and dried under a nitrogen atmosphere.

[0043] Graphite carbon rods are used as graphite electrode materials, cut to its radius r=0.4cm, length l=5.5cm, and prepare 4 graphite carbon rods of the same specification. Then wrap one en...

Embodiment 2

[0055] AchE / GQDs / N-F-TiO 2 Photocurrent Response of Ns Photoelectrochemical Biosensor to ATCl

[0056] Acetylthiocholine chloride (ATCl) can be used as the substrate of AchE to catalyze the hydrolysis, and the product is thiocholine (TCh), and TCh can be used as a donor of electron sacrifice, and theoretically, an oxidation reaction occurs on the hole, effectively Capture photogenerated holes, inhibit the recombination rate of photogenerated holes and photogenerated electrons, thereby increasing the photocurrent and increasing the amplified signal. Apply ATCl to the AchE / GQDs / N-F-TiO prepared in Example 12 On the Ns photoelectrochemical biosensor, it is verified whether the biosensor of the present invention can have a current response.

[0057] With the AchE / GQDs / N-F-TiO prepared in embodiment 1 2 The Ns photoelectrochemical biosensor is the working electrode, the saturated calomel electrode is the reference electrode, and the platinum wire is the auxiliary electrode. The v...

Embodiment 3

[0060] AchE / GQDs / N-F-TiO 2 Detection of the Inhibitory Effect of Ketamine on AchE by Ns Photoelectrochemical Biosensor

[0061] AchE / GQDs / N-F-TiO prepared with embodiment 1 2 The Ns photoelectrochemical biosensor is the working electrode, the saturated calomel electrode is the reference electrode, and the platinum wire is the auxiliary electrode. The voltage of the working electrode in the photoelectrochemical cell is set to +0.6V, at a wavelength of 365nm, in phosphoric acid at pH=7.00 In salt buffer solution, with 500W xenon lamp as light source, using chronoamperometry, according to AchE / GQDs / N-F-TiO 2 The photocurrent change of ATCl when there is ketamine in the Ns photoelectrochemical biosensor to explore the inhibition of ketamine on AchE.

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Abstract

The invention relates to the technical field of electrochemical analysis, in particular to an AchE/GQDs/N-F-TiO2Ns photoelectrochemical biosensor preparation method and application thereof. The AchE/GQDs/N-F-TiO2Ns photoelectrochemical biosensor comprises GQDs/N-F-TiO2Ns and AchE, wherein the GQDs/N-F-TiO2Ns comprises a nitrogen and fluorine-doped titanium dioxide nanotube array (N-F-TiO2Ns) and graphene quantum dots (GQDs) modified on the titanium dioxide nanotube array; cross-linking modification of acetyl cholinesterase (AchE) on the GQDs/N-F-TiO2Ns is carried out; the wavelength at the highest photocurrent response of the photoelectrochemical biosensor is 365 nm; and cross-linking modification of the AchE on the GQDs/N-F-TiO2Ns is carried out through chitosan. The modification of the graphene quantum dots improves the photocurrent intensity. Due to the good biocompatibility, the immobilization of the enzyme on an electrode is improved, and the affinity of the enzyme to a substrateis improved at the same time. the method can also be used in detection study related to acetylcholine and acetyl cholinesterase.

Description

technical field [0001] The invention relates to the technical field of electrochemical analysis, in particular to an AchE / GQDs / N-F-TiO 2 Preparation method and application of Ns photoelectrochemical biosensor. Background technique [0002] Acetylcholine (Ach) is an important neurotransmitter that exists in the peripheral nervous system and central nervous system of many organisms; Ach plays an important role in memory and cognitive functions, and controls skeletal muscle and The movement of the heart muscle. Therefore, its concentration is too high or too low can lead to the occurrence of diseases, such as schizophrenia. Schizophrenia (SZ) is a chronic debilitating neuropsychiatric disease and is considered to be the main cause of chronic disability. Patients with schizophrenia are often found to have cognitive dysfunction related to changes in the cholinergic system. [0003] Acetylcholinesterase (AchE) is a key enzyme in biological nerve transmission. In cholinergic syn...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3271
Inventor 黄齐林陆汉星韩汝莲徐丽李婷单永芳曾艳萍钟兴航杨星月林洪李明文云波
Owner YUXI NORMAL UNIV
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