A test strip for rapid detection of pesticides

A technology of test strips and pesticides, applied in the field of analytical chemistry, can solve rare problems, achieve the effects of improving stability and simplifying preparation steps

Active Publication Date: 2021-06-04
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some urine sugar test strips, HIV test strips, and drug detection test strips have been reported, but there are few reports on test strips that generate nanozymes in situ and combine them with smartphones for pesticide detection

Method used

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  • A test strip for rapid detection of pesticides
  • A test strip for rapid detection of pesticides
  • A test strip for rapid detection of pesticides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation of test strips for pesticide detection

[0034] (1) Use a paper cutter to cut the filter membrane into filter membrane strips with a size of 0.2×2.5 cm;

[0035] (2) Use clips to fix both ends of the filter strip, and leave a 2 mm gap at the lower edge of the filter strip at 1-2 cm for the preparation of subsequent test strips;

[0036] (3) Add 10% bovine serum albumin (BSA) solution, 1 mmol / L NHS, 0.5 mmol / L EDC equal volume mixture dropwise into the gap of the above test strip (BSA is used to improve the Adsorption capacity and as a template for the next reaction, EDC / NHS is to activate the carboxyl group on BSA), after incubation at room temperature for 30 min, washed with water and dried;

[0037] (4) Add 4 mmol / L FeCl 3 , 2 mmol / L FeCl 2 and 16 mmol / L of ammonia water are uniformly dispersed in the filter membrane gap obtained in the previous step in order to generate in situ ferric oxide imitative enzyme material (Fe 3 o 4 @BSA), after incubation ...

Embodiment 2

[0040] The detection area in the test strip has the activity of AchE, ChOx and imitation peroxidase under weak acidic conditions, AchE can catalyze Ach to generate choline, and ChOx can catalyze choline to generate H 2 o 2 , the mimic peroxidase catalyzes H 2 o 2 A color reaction occurs with an organic chromogen, and when the pesticide is present, it will irreversibly inhibit the activity of AchE, resulting in a cascade of H 2 o 2 decrease, the color of the organic chromogenic agent becomes lighter, and the detection schematic diagram is shown in the appendix of the instruction manual. figure 1 .

[0041] Verification of the inhibitory effect of active pesticides on test strips:

[0042] Experimental system a: The catalytic reaction system is a sample solution containing Ach (0.1 mmol / L), fragments of test strips obtained in Example 1, organic chromogen TMB (0.5 mmol / L) and acetate buffer (pH 4.0, 100 mmol / L). After immersing the detection strip in the buffer solution c...

Embodiment 3

[0046] Qualitative analysis of OP

[0047] Specific system: Prepare two previously prepared test strips, one of which is dipped into the weakly acidic solution containing Ach (1mM) and TMB (0.5 mmol / L), and the other dipped into After 2-5 minutes, observe the colors of the two test papers in a weakly acidic solution containing OP (50 pmol / L), Ach (1 mmol / L) and TMB (0.5 mmol / L). As attached to the manual image 3 As shown, the color of the test strip without OP is darker than that with OP, indicating that the test strip prepared by this scheme can be used for qualitative detection of OP.

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Abstract

The invention relates to the fields of nanometer catalysis, environmental protection and analytical chemistry, and specifically includes a test strip for rapidly detecting pesticides. In-situ generation of iron ferric oxide nanoparticles with imitation peroxidase activity on test strips and modification of acetylcholinesterase and choline oxidase, when organophosphorus pesticides exist, it will irreversibly inhibit the activity of acetylcholinesterase , so that the amount of choline generated by the hydrolysis of acetylcholine is reduced, resulting in the reduction of hydrogen peroxide generated through the cascade reaction, and the color development of the chromogenic substrate becomes lighter. Moreover, the chromatographic phenomenon of the test paper can eliminate the interference of other substances in the sample. The convenient and quantitative detection of acetylcholine can be realized through mobile phone camera and mobile phone software analysis. The invention can be used in the fields of pesticide residue analysis, biomedicine, environmental monitoring and the like.

Description

technical field [0001] The invention relates to the field of analytical chemistry, and specifically includes a test strip for rapidly detecting pesticides. [0002] technical background [0003] Organophosphorus pesticides (Organophosphorus Pesticide, OP) are the most widely used agricultural pesticides, which play an important role in improving agricultural productivity and preventing pests and diseases. However, organophosphorus pesticides can inhibit the activity of acetylcholinesterase in the human body and cause various human diseases, such as Alzheimer's disease, Parkinson's disease and stroke. At the same time, due to the high residue characteristics of organophosphorus pesticides and the serious overuse or irregular operation in agricultural production, the high content of pesticide residues on agricultural products seriously threatens the balance of the ecological environment. Therefore, it is of great practical application value to build a high-sensitivity detectio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 韩磊吴加慧
Owner QINGDAO AGRI UNIV
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