Preparation of high-purity fructo oligosaccharides

A kind of fructooligosaccharide, high-purity technology, applied in fermentation and other directions, can solve the problems of limited application scope of fructooligosaccharides, and achieve the effects of stable product quality, less dosage and simple process

Inactive Publication Date: 2009-03-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ordinary grade fructo-oligosaccharide contains digestible sugars such as by-product glucose, fructose and the remaining reaction substrate sucrose, whi...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Get 10 liters of fructooligosaccharides in the membrane enzyme reactor, the amount of fructotransferase enzyme is 2U / g, the enzyme reaction temperature is 45 °C, the pH is 4.0, and the operating pressure is 1.1MPa, so that disaccharides are converted into fructooligosaccharides and monosaccharides. Sugar and monosaccharide are filtered out through the membrane to obtain high-purity fructooligosaccharides; then the separated monosaccharide solution is processed through a nanofiltration (or reverse osmosis) system to separate the monosaccharide from water and achieve concentration of the monosaccharide , the water can be reused in the membrane enzyme reactor.

Embodiment 2

[0013] Get 10 liters of fructooligosaccharide in the membrane enzyme reactor, the amount of fructotransferase enzyme is 4.2U / g, the enzyme reaction temperature is 50 ℃, the pH is 5.5, and the operating pressure is 2.3MPa, so that the disaccharide is converted into fructooligosaccharide and Monosaccharides are filtered out through the membrane to obtain high-purity fructo-oligosaccharides; then the separated monosaccharide solution is treated by a nanofiltration (or reverse osmosis) system to separate the monosaccharides from water, and the monosaccharides reach Concentrated, the water can be reused in the membrane enzyme reactor.

Embodiment 3

[0015] Get 15 liters of fructo-oligosaccharides in the membrane enzyme reactor, the amount of fructotransferase enzyme is 5U / g, the enzyme reaction temperature is 10°C, the pH is 7.0, and the operating pressure is 3.2MPa, so that disaccharides are converted into fructo-oligosaccharides and monosaccharides Sugar and monosaccharide are filtered out through the membrane to obtain high-purity fructooligosaccharides; then the separated monosaccharide solution is processed through a nanofiltration (or reverse osmosis) system to separate the monosaccharide from water and achieve concentration of the monosaccharide , the water can be reused in the membrane enzyme reactor.

[0016] Table 1. Membrane enzyme reactor prepares the effect of high-purity fructo-oligosaccharide

[0017]

[0018] As can be seen from the examples and data, the purity of fructooligosaccharides is greatly improved after passing through the membrane enzyme reactor, and the permeate through the nanofiltra...

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PUM

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Abstract

The invention relates to a preparation method of high-purity low fructosan, which belongs to the technical field of purifying low fructosan by organically integrating barrier separation and enzyme reaction. In the invention, common low fructosan serves as the raw material; enzyme reaction and barrier separation are conducted in a barrier and enzyme reactor, the content of monosaccharide and sucrose is reduced to obtain the high-purity low fructosan. The content of the low fructosan obtained through the method is above 95 percent; the method has the advantages of convenient operation, stable quality, safety and reliability, and is applicable to industrialized production.

Description

technical field [0001] The invention relates to a method for preparing high-purity fructo-oligosaccharides, that is, a method for preparing high-purity fructo-oligosaccharides by using a membrane enzyme reactor. The invention belongs to the technical field of organically combining membrane separation and enzyme reaction to purify fructo-oligosaccharides. Background technique [0002] Among food additives and sweeteners, fructooligosaccharide is the first functional oligosaccharide to pass the FDA review as a generally recognized safety level (GRAS), and its low caries, indigestibility and bifidobacteria proliferation have been confirmed . As a non-digestible functional food, fructooligosaccharide is a typical bifidus factor that meets the standard of prebiotics (i.e. bifidobacterium probiotics), which can regulate the flora in the body in two directions, reduce blood lipids, promote vitamin synthesis, protect the liver, and promote Ca , Mg, Fe and other mineral absorption,...

Claims

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Application Information

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IPC IPC(8): C12P19/00C12P19/18
Inventor 江波张涛翁桂华
Owner JIANGNAN UNIV
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