Preparation of high-purity fructo oligosaccharides
A kind of fructooligosaccharide, high-purity technology, applied in fermentation and other directions, can solve the problems of limited application scope of fructooligosaccharides, and achieve the effects of stable product quality, less dosage and simple process
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Embodiment 1
[0011] Get 10 liters of fructooligosaccharides in the membrane enzyme reactor, the amount of fructotransferase enzyme is 2U / g, the enzyme reaction temperature is 45 °C, the pH is 4.0, and the operating pressure is 1.1MPa, so that disaccharides are converted into fructooligosaccharides and monosaccharides. Sugar and monosaccharide are filtered out through the membrane to obtain high-purity fructooligosaccharides; then the separated monosaccharide solution is processed through a nanofiltration (or reverse osmosis) system to separate the monosaccharide from water and achieve concentration of the monosaccharide , the water can be reused in the membrane enzyme reactor.
Embodiment 2
[0013] Get 10 liters of fructooligosaccharide in the membrane enzyme reactor, the amount of fructotransferase enzyme is 4.2U / g, the enzyme reaction temperature is 50 ℃, the pH is 5.5, and the operating pressure is 2.3MPa, so that the disaccharide is converted into fructooligosaccharide and Monosaccharides are filtered out through the membrane to obtain high-purity fructo-oligosaccharides; then the separated monosaccharide solution is treated by a nanofiltration (or reverse osmosis) system to separate the monosaccharides from water, and the monosaccharides reach Concentrated, the water can be reused in the membrane enzyme reactor.
Embodiment 3
[0015] Get 15 liters of fructo-oligosaccharides in the membrane enzyme reactor, the amount of fructotransferase enzyme is 5U / g, the enzyme reaction temperature is 10°C, the pH is 7.0, and the operating pressure is 3.2MPa, so that disaccharides are converted into fructo-oligosaccharides and monosaccharides Sugar and monosaccharide are filtered out through the membrane to obtain high-purity fructooligosaccharides; then the separated monosaccharide solution is processed through a nanofiltration (or reverse osmosis) system to separate the monosaccharide from water and achieve concentration of the monosaccharide , the water can be reused in the membrane enzyme reactor.
[0016] Table 1. Membrane enzyme reactor prepares the effect of high-purity fructo-oligosaccharide
[0017]
[0018] As can be seen from the examples and data, the purity of fructooligosaccharides is greatly improved after passing through the membrane enzyme reactor, and the permeate through the nanofiltra...
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