Protein doped organic-inorganic hybridized flower-shaped nano material

A technology of nanomaterials and proteins, applied in the directions of immobilization on or in inorganic carriers, chemical instruments and methods, carrier binding/immobilization of peptides, etc., to achieve the effects of good use value and application prospects, simple operation and low cost of raw materials

Inactive Publication Date: 2013-09-11
FUZHOU UNIV
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a protein-doped organic-inorganic hybrid flower-like nanomaterial and a preparation method thereof. The synthesis method of the present invention is simple, the conditions are mild, the reagents are easy to obtain, and the shortcomings of the existing preparation methods such as harsh conditions are overcome.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Protein doped organic-inorganic hybridized flower-shaped nano material
  • Protein doped organic-inorganic hybridized flower-shaped nano material
  • Protein doped organic-inorganic hybridized flower-shaped nano material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Synthesis of trypsin-doped flower-like nanomaterials

[0028] Synthesis steps of trypsin-doped flower-like nanomaterials:

[0029] 1. Prepare 0.02, 0.10, 0.50, 1.00, 2.00, 3.00 mg / mL trypsin phosphate buffer solution (KCl, NaCl, NaCl, 2 HPO 4 12H 2 O, KH 2 PO 4 concentration of 0.1 mol / L, pH 7.40) and 0.12 mol / L copper sulfate pentahydrate solution.

[0030] 2. Take 4.5 mL of the protein solution in step 1 into a centrifuge tube, add 0.030 mL of the copper sulfate solution obtained in step 1, mix well, and place it in a 25°C water bath for 72 hours of constant temperature reaction.

[0031] 3. Wash the product obtained in step 2 with 10 mL of deionized water, and then separate the product by centrifugation. Repeat the washing process 3 times. The washed product was dried under vacuum at room temperature for 5 hours.

Embodiment 2

[0032] Example 2 Synthesis of flower-like nanomaterials doped with lysozyme

[0033] The trypsin solution in Example 1 was replaced by lysozyme solution (1.00 mg / mL), and the steps in Example 1 were repeated to obtain lysozyme-doped flower-like nanomaterials.

Embodiment 3

[0034] Example 3 Preparation of flower-like nanomaterials doped with immunoglobulin G

[0035] The trypsin solution in Example 1 was replaced by immunoglobulin G solution (1.00 mg / mL), and the steps in Example 1 were repeated to obtain immunoglobulin-doped flower-like nanomaterials.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention discloses a protein doped organic-inorganic hybridized flower-shaped nano material and a preparation method thereof, and belongs to the technical field of preparation of organic-inorganic compound materials. The material is of a three-dimensional flower-shaped nano structure. The organic-inorganic hybridized flower-shaped nano material which is 5-50 mu m in diameter is obtained by taking protein, phosphate and soluble bivalent copper ion salt as raw materials in an aqueous liquor by reaction at 0-50 DEG C. The material provided by the invention has the advantages of simplicity in operation, low raw material cost, environmental friendliness and the like. The protein doped organic-inorganic hybridized flower-shaped nano material prepared by the invention has good using value and application prospect in the fields of biosensors, enzyme reactors and the like.

Description

technical field [0001] The invention belongs to the technical field of preparation of organic-inorganic composite materials, relates to nanomaterials, in particular to a protein-doped organic-inorganic hybrid flower-like nanomaterial and a preparation method thereof. Background technique [0002] Due to the special properties of large specific surface area, nanomaterials have broad application prospects in the fields of light, electricity, magnetism, chemistry and life sciences. Organic-inorganic hybrid nanomaterials are the fourth generation of new materials after single-component materials, composite materials and functionally graded materials. The controllable properties of molecules and atoms have gradually become one of the most promising research directions in materials science. Because it introduces different inorganic elements and organic ligands or polymers into the system, the material not only has the relevant properties of the inorganic active center or organic ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C07K17/14
Inventor 林子俺肖云郑江南杨黄浩陈国南
Owner FUZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap