Synthetic method of beta-alanine

Abstract

The invention discloses a synthetic method of beta-alanine. The method comprises the steps: a) preparation of a substrate: filling 5g/L-80g/L L-aspartic acid substrate solutions into an enzyme reactor, and regulating pH into 5.0-8.5 by using alkalizers; b) enzymic catalytic reaction: adding enzyme-containing bacterium solutions into the substrate solutions for performing catalytic reaction, adding a proper amount of L-aspartic acid in batch during reaction to regulate pH of a reactant to 5.0-9.0, and performing fermentation culture, condensation and pulverization on the enzyme-containing bacterium solutions by utilizing engineering bacteria of high-yield L-aspartic acid alpha-decarboxylase; and c) filtration, decoloring and crystallizing treatment of the reactant in the enzyme reactor to obtain beta-alanine. L-aspartic acid is further catalyzed by using the enzyme-containing bacterium solutions and converted into beta-alanine, and the beta-alanine is mild in production condition, simple in process, low in environment pollution, high in quality of products and convenient to extract at the downstream; the purity reaches 99.0 percent, and the conversion rate of beta-alanine reaches 99.5 percent.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for synthesizing beta-alanine. Background technique [0002] β-alanine (β-alanine), namely 3-aminopropanoic acid (3-aminopropanoic), is the only β-type non-protein amino acid that exists in nature. As an important biochemical raw material, β-alanine is used in It has a very wide range of applications in the fields of medicine, feed and food. [0003] In the prior art, domestic and foreign enterprises mainly produce β-alanine with chemical synthesis methods, such as acrylonitrile method, β-aminopropionitrile method and succinimide degradation method, but adopt the above-mentioned chemical synthesis method to produce β-alanine Alanine will pollute the environment more seriously, especially the use of nitrile-containing raw materials, which is extremely toxic to the environment and organisms. [0004] In order to overcome the above-mentioned technical defects, according to re...

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Problems solved by technology

[0003] In the prior art, domestic and foreign enterprises mainly produce β-alanine with chemical synthesis methods, such as acrylonitrile method, β-aminopropionitrile method and succinimide degradation method, but adopt the above-mentioned chemical synthesis method to produce β-alanine Alanine will pollute the environment more seriously, especially the use of nitrile-containing raw materials, which is extremely toxic to the environment and organisms

[0004] In order to overcome the above-mentioned technical defects, according to research reports, many enterprises have adopted biosynthesis to produce β-alanine, such as: using Sarcina luteus to convert acrylic acid into β-alanine, β-alanine The conversion rate of Escherichia coli is about 54%; Escherichia coli BL21 (DE3) is selected as the host to express the L-aspartic acid-alpha carboxylase derived from Escherichia coli; The invention patent of "method for synthesizing β-alanine" (publication number is CN102851333A), the technical scheme disclosed in this invention patent uses fumaric acid as a substrate to use enzyme-containing Escherichia coli cells to catalyze the production of β-alanine , but this method not only has complex process conditions and low conversion rate, but also has a long production cycle and many by-products

[0005] It can be seen that the methods for producing β-alanine in the prior art either cause serious environmental pollution, or have defects such as low conversion rate of β-alanine and long production cycle. Therefore, how to improve the conversion of β-alanine rate, to achieve low-pollution and high-efficiency production of β-alanine, which still needs to be further studied by researchers