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Production method for coproducing unsaturated monoglyceride by using diglyceride enzyme method

A technology of diglyceride enzyme and production method, which is applied in the field of enzymatic production of unsaturated monoglyceride and diglyceride, can solve the problems of high difficulty, low reaction efficiency, inconvenient use of carrier regeneration, etc., and achieve the solution of acid Price issues, the effect of simplifying production operations

Active Publication Date: 2013-10-23
YHSN TECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Practice has found that although glycerol in the form of adsorption can overcome the defects that free glycerol is difficult to react, it still has the disadvantages of low reaction efficiency and inconvenient use of carrier regeneration, and it is very difficult to implement industrialization

Method used

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  • Production method for coproducing unsaturated monoglyceride by using diglyceride enzyme method
  • Production method for coproducing unsaturated monoglyceride by using diglyceride enzyme method
  • Production method for coproducing unsaturated monoglyceride by using diglyceride enzyme method

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Experimental program
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Effect test

Embodiment 1

[0029]A method for enzymatically producing diglycerides by co-producing unsaturated monoglycerides, comprising the following steps:

[0030] Step (1), mix 5kg soybean oil and 0.3kg absolute ethanol, add 2g of immobilized lipase Novozyme 435 (supplied by Novozymes, Denmark), stir and react at 35°C for 3 hours, the formation rate of fatty acid ethyl ester is 20.6%, vacuum desorption Residual ethanol is removed to obtain alcoholysis products.

[0031] In step (2), 3kg of alcoholysis product is loaded into such as figure 1 In the raw material storage tank 1 shown, add 0.3kg glycerin, the material circulates through the packed bed enzyme reactor 3 of immobilized lipase under the action of pump 2, the reaction temperature is 60 ℃, adopts immobilized lipase Novozyme 435, the enzyme The filling amount is 10g, react under normal pressure for 1 hour, then control the absolute pressure of the reaction to 10pa, and then discharge after reacting for 3 hours. The composition of the analysi...

Embodiment 2

[0034] A method for enzymatically producing diglycerides by co-producing unsaturated monoglycerides, comprising the following steps:

[0035] Step (1), mix 5kg soybean oil and 0.3kg absolute ethanol, add 2g of immobilized lipase Novozyme 435 (provided by Novozymes, Denmark), stir and react at 35°C for 0.5 hours, the ethyl ester formation rate is 9.8%, and vacuum remove Ethanol remains to obtain alcoholysis products.

[0036] In step (2), 3kg of alcoholysis product is loaded into such as figure 1 Add 0.15kg of glycerol to the raw material storage tank shown, and the material circulates through the packed bed enzyme reactor of immobilized lipase under the action of the pump. The reaction temperature is 60°C, and the immobilized lipase Novozyme 435 is used. It was 10g, first reacted under normal pressure for 6 hours, then the absolute pressure of reaction was controlled to be 100pa, and discharged after 24 hours of reaction, its composition was analyzed in Table 1.

[0037] In ...

Embodiment 3

[0039] A method for enzymatically producing diglycerides by co-producing unsaturated monoglycerides, comprising the following steps:

[0040] Step (1), mix 5kg of soybean oil and 0.3kg of absolute ethanol, add 2g of immobilized lipase Novozyme 435 (provided by Novozymes, Denmark), stir and react at 35°C for 5 hours, the measured ethyl ester formation rate is 31.2%, vacuum removal Ethanol remains to obtain alcoholysis products.

[0041] In step (2), 3kg of alcoholysis product is loaded into such as figure 1 Add 0.45kg of glycerol to the raw material storage tank shown, and the material circulates through the packed bed enzyme reactor of immobilized lipase under the action of the pump. The reaction temperature is 60°C, and the immobilized lipase Novozyme 435 is used. It was 10g, first reacted under normal pressure for 3 hours, then the absolute pressure of the reaction was controlled to be 200pa, and the material was discharged after 12 hours of reaction, and its composition wa...

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Abstract

The invention discloses a production method for coproducing unsaturated monoglyceride by using a diglyceride enzyme method. The method comprises: (1)adding absolute ethyl alcohol into a natural grease, carrying out an alcoholysis reaction under catalysis of immobilized lipase, making the generation rate of fatty acid ethyl ester to be 5-40%w / w, and removing the residual alcohol to obtain an alcoholysis product; (2) adding 5-15%w / w of glycerin into the alcoholysis product of step (1), then reacting the above mixed substrates in a packed-bed enzyme reactor filled with immobilized lipase under a vacuum condition; and (3) performing molecular distillation on the reaction product of step (2), and separating to obtain a high-temperature fraction which is a diglyceride-containing product and a low-temperature fraction which mainly comprises monoglyceride. The reaction system of the invention needs no water, therefore the problem of product acid value is solved, and then coproduction of monoglyceride is realized; and the glycerin does not need preadsorption processing, and therefore the production operation is simplified.

Description

technical field [0001] The invention relates to an enzymatic production method of unsaturated monoglyceride and diglyceride. Background technique [0002] The main components of natural edible oils and fats are triglycerides, which are compounds consisting of three fatty acid molecules bound to one glycerol. In natural oils and fats, there is also a small amount of diglyceride. Diglyceride is a compound formed by connecting two molecules of fatty acid to one glycerol molecule. The content of diglyceride in edible oil usually does not exceed 5%. In 1993, Hara K reported for the first time that diacylglycerol has the function of lowering blood lipids (Ann Nutr Metab1993; 37:185), suggesting that it can be used to prevent and treat hyperlipidemia and cardiovascular and cerebrovascular diseases closely related to hyperlipidemia . Japan's Kao Corporation first launched a diglyceride-based edible oil product in the late 1990s and achieved commercial success. Since then, more an...

Claims

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Application Information

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IPC IPC(8): C12P7/64
Inventor 李响宁柠王卫飞王永华杨博蓝东明陈华勇
Owner YHSN TECH LTD
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