38 results about "Fatty acids.ethyl esters" patented technology

The present application relates to the field of biochemical engineering, and discloses a composition containing eicosapentaenoic acid ethyl ester, a preparation method therefor, and a use thereof. The preparation method for the composition containing eicosapentaenoic acid ethyl ester disclosed in the present application comprises the following steps: transesterification, multi-stage molecular distillation, urea inclusion, rectification and preparative chromatographic purification. In the present application, by means of performing molecular distillation and urea inclusion before rectification, a liquid mixture can be separated, removing high-boiling-point saturated fatty acid ethyl esters and most fatty acid ethyl esters with one double bond, thereby effectively lowering the rectification temperature and preventing the formation of excessive isomers. Furthermore, the composition obtained by means of the described steps can achieve an eicosapentaenoic acid ethyl ester content of as high as 96.5 area% to 99.5 area%, with low isomer and impurity content, making it suitable for use in the preparation of food and pharmaceuticals.

The invention belongs to the technical field of food, and discloses a method, a system, equipment and a medium for analyzing interaction of turbid components in Baijiu based on an S curve, and the method comprises the following steps: respectively determining actually measured visual thresholds of a chemical substance A, a chemical substance B and a mixed chemical substance AB in the Baijiu by utilizing an S curve method; determining a theoretical threshold value of the mixed chemical substance AB based on an addition formula; and based on the ratio of the actually measured visual threshold value of the mixed chemical substance AB to the theoretical threshold value of the mixed chemical substance AB, analyzing the interaction between different chemical substances in the mixed chemical substance AB. According to the technical scheme provided by the invention, the mutual synergistic effect between the higher fatty acid ethyl esters can be intuitively reflected.

The invention discloses a preparation method of omega-3 fatty acid ethyl ester 90 soft capsules, which comprises the following steps: 1) preparing raw materials and auxiliary materials: weighing gelatin, glycerol, purified water and omega-3 fatty acid ethyl ester 90 according to prescription dosage for later use; (2) preparing a glue solution, namely heating purified water to 80 DEG C, sequentially adding glycerol and gelatin for capsules, stirring until the gelatin for capsules is melted, carrying out vacuum stirring and degassing for more than 30 minutes, and standing and degassing for 30-60 minutes; 3) pelleting: adjusting the temperature of a capsule box, the temperature of a spraying body, the rotating speed of a main shaft and the refrigeration temperature of a roller, adjusting the thickness of a wet capsule skin, adding the omega-3 fatty acid ethyl ester 90 prepared in the step 1) into a hopper, and starting pelleting; and 4) drying: controlling the temperature of a drying chamber at 10-30 DEG C, controlling the humidity below 40%, and drying until the water content of the capsule skin is less than 13% to obtain the omega-3 fatty acid ethyl ester 90 soft capsule, the process is simple, and the prepared omega-3 fatty acid ethyl ester 90 soft capsule has good disintegration effect and stable performance.

The present application belongs to the field of food oil, and particularly relates to a method for processing olive oil, especially virgin olive oil. The method comprises the following steps: cleaning, drying and crushing olive pomace after oil extraction by using an organic solvent (n-hexane or ethanol), adding a certain amount of olive pomace powder into olive oil and stirring under vacuum, then removing the precipitate, and finally processing under ultrahigh pressure. The content of ethyl ester of fatty acid in the virgin olive oil processed by the method of the present application can be kept less than or equal to 35 mg / kg under the condition of 30 DEG C and packaging in the dark for 12-24 months.

The disclosure relates to emollient compositions and a method of making the same. The emollient composition comprises a mixture of cocoa butter fatty acid ethyl esters. The method comprises the steps of: (a) providing cocoa butter and ethyl alcohol; and (b) mixing the cocoa butter and the ethyl alcohol in the presence of an esterification catalyst at an esterification temperature to obtain the cocoa butter fatty acid ethyl esters. A personal care product comprising the emollient composition is also disclosed herein.

The invention discloses glyceride rich in fish oil omega-3-PUFA and a preparation method thereof.The preparation method comprises the steps that 1, refined fish oil, ethyl alcohol and glycerin are subjected to transesterification under the catalytic action of a basic catalyst, and part of ethanol hydrolysis oil is obtained; (2) carrying out crystallization fractionation on a part of the ethanol hydrolysis oil to obtain a PUFA part glyceride enriched product; (3) preheating a PUFA part of glyceride enrichment, molecular distillation grade omega-3-PUFA ethyl ester and optional glycerol to an enzymatic transesterification reaction temperature, and carrying out vacuum homogeneous mixing; then adding lipase, and carrying out ester exchange reaction; after the reaction is finished, residual fatty acid ethyl ester is removed from an obtained reaction product through molecular distillation, and glyceride rich in fish oil omega-3-PUFA is obtained. The method can solve the technical problems of low conversion efficiency and large lipase consumption in the process of producing the oil ester rich in fish oil omega-3-PUFA by taking molecular distillation-grade fish oil omega-3-PUFA ethyl ester as a main raw material.

The invention discloses a collophanite flotation combined collecting agent and a preparation method and application thereof. The phosphorite flotation combined collecting agent comprises agent components and water with the same mass, and the agent components are composed of, by mass, 40%-85% of mixed amine, 8%-30% of a mixed nonionic foaming agent and 7%-30% of a hydrophobic accelerant; the mixed amine is a mixture of N-dodecyl ethanolamine and alkoxylated polyethyleneimine; the mixed nonionic foaming agent is a mixture of methyl isobutyl carbinol and C5-C9 fatty acid ethyl ester; the hydrophobic accelerant is a mixture of polymethylhydrosiloxane and kerosene. The components of the collecting agent are optimized, the synergistic effect of all the components is exerted, the using amount of the collecting agent in the reverse flotation desilicication process is reduced, the production cost is reduced, and the flotation froth suitability and the flotation effect are improved.

The invention belongs to the field of wine brewing, and relates to a rosa sterilis and crystal grape mixed fruit wine rich in fatty acid ethyl ester and a preparation method of the rosa sterilis and crystal grape mixed fruit wine. According to the preparation method, the rosa sterilis and crystal grape mixed fruit juice is taken as a main raw material, fermentation is carried out by virtue of Saccharomyces cerevisiae XJ-YSSCE010-1, flavor substances such as ethanol and fatty acid ethyl ester are generated, and the rosa sterilis and crystal grape mixed fruit wine rich in fatty acid ethyl ester is prepared. The saccharomyces cerevisiae XJ-YSSCE010-1 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the saccharomyces cerevisiae XJ-YSSCE010-1 is CGMCC (China General Microbiological Culture Collection Center) No. 31174. The preparation method disclosed by the invention is beneficial to increasing the content of flavor substances such as fatty acid ethyl ester in the rosa sterilis and crystal grape mixed fruit wine.

Disclosed herein are methods of processing an oil composition. The oil composition comprises: 40-100 wt% oil, 0-20 wt% ethanol and 0-25 wt% water. The oil comprises 95-99 wt% triacylglycerides, 1-5 wt% diacylglycerides, and less than 1 wt% monoacylglycerides. The methods disclosed herein may comprise adding water and a base to the oil composition or adding water and an enzyme to the oil composition. Such methods comprise hydrolysing at least some of the diacylglycerides and / or triacylglycerides to obtain an oil comprising at least 10 wt% monoacylglycerides and / or at least 10 wt% diacylglycerides. The methods disclosed herein may comprise adding ethanol and a base to the oil composition. Such methods comprise transesterification of at least some of the monoacylglycerides, diacylglycerides and / or triacylglycerides to obtain an oil comprising at least 20 wt% fatty acid ethyl esters (FAEE). Also provided herein are products obtainable by the disclosed methods.

Methods for using silica-zircone catalysts in processes for reducing an amount of glycidol, glycidyl ester(s), or both glycidol and glycidyl ester(s) from a fatty acid ethyl ester-containing composition are disclosed. Silica-zircone catalysts and methods for manufacturing silica-zircone catalysts are also disclosed.

PROCESS FOR PRODUCING A JET FUEL FROM FEEDSTOCKS OF RENEWABLE ORIGIN The invention relates to a process for producing a jet fuel comprising at least the steps of: a) providing fatty acid ethyl esters from the reaction of animal fats and / or waste oils with methanol in a transesterification reactor, b) providing hydrocarbons of fossil origin, c) preparing a hydrocarbon feedstock containing the hydrocarbons of fossil origin provided by step b) and the fatty acid ethyl esters provided by step a) in a content of at most 5% by volume relative to the hydrocarbon feedstock, d) subjecting the hydrocarbon feedstock prepared in step c) to hydrotreatment and obtaining a treated hydrocarbon feedstock, e) fractionating the treated hydrocarbon feedstock obtained in step d) and recovering a kerosene fraction as jet fuel. Figure 1

Methods of using silica-zirconia catalysts in processes to reduce an amount of glycidol, glycidyl ester(s), or both glycidol and glycidyl ester(s) from a fatty acid ethyl ester-containing composition are disclosed. The fatty acids are preferably omega-3 or omega-6 fatty acids such as ERA and DHA. Silica-zirconia catalysts and methods of making silica-zirconia catalysts are also disclosed.

The invention discloses an esterification reaction system and a cyclic esterification reaction method. The system comprises a batching kettle, the batching kettle passes through a glycerol feeding end, a high-pressure nitrogen feeding end and a fatty acid ethyl ester feeding end, a reaction tank, a stirring shaft, a fan-shaped stirring paddle and a stirring pipe arranged close to the inner wall are arranged in the batching kettle, a first filter plate and a vertical second filter plate are arranged at the bottom of the batching kettle and used for separating enzyme from a product, and high-pressure nitrogen can be introduced into a nitrogen nozzle at the bottom of the batching kettle. The fatty acid ethyl ester feeding end is communicated with the pretreatment kettle and the ethanol tank, so that the esterification pretreatment of high-acid-value fatty acid is realized; the top of the batching kettle is communicated with a condenser through a vacuum pipeline and a vacuum pump, and ethanol and redundant nitrogen generated in the reaction are extracted and condensed and then are respectively recovered to an ethanol tank and a nitrogen tank for cyclic utilization. The method comprises the following steps: continuously adding raw materials according to set steps, pressurizing nitrogen, heating and stirring, vacuumizing and condensing, recycling ethanol and nitrogen, separating enzyme through two-stage filtration, and refluxing unreacted materials until the high-quality triglyceride type fish oil is obtained.

The invention discloses a hydrolase E632 coding gene for synthesizing a plurality of fatty acid ethyl esters from Azoarcus indigens and an application of the hydrolase E632 coding gene. The amino acid sequence of the hydrolase E632 is as shown in SEQ ID NO.1, and the nucleotide sequence of the coding gene of the hydrolase E632 is as shown in SEQ ID NO.2. The invention belongs to the technical field of hydrolase engineering, particularly discloses construction of an Azoarcus indigens source hydrolase E632 coding gene and an escherichia coli expression vector carrying the gene, and further discloses application of the Azoarcus indigens source hydrolase E632 in catalytic synthesis of ethyl valerate, ethyl hexanoate, ethyl caprylate and ethyl caprate in a water phase system. The invention firstly provides a hydrolase E632 which is derived from Azoarcus indigens and has the capability of catalytically synthesizing fatty acid ethyl ester in a water phase system and a coding gene of the hydrolase E632.

The present disclosure relates to an emollient composition and a method for making the same. The emollient composition comprises a mixture of cocoa butter fatty acid ethyl esters. The method includes the steps of (a) providing cocoa butter and ethyl alcohol, and (b) combining the cocoa butter and ethyl alcohol in the presence of an esterification catalyst at an esterification temperature to obtain the cocoa butter fatty acid ethyl esters. Personal care products comprising the emollient composition are also disclosed herein.

A method for preparing phospholipid type DHA, comprising the following steps: (1) hydrolysis of phospholipid to obtain hydrolyzed phospholipid; (2) preparation of DHA ethyl ester: slowly adding NaOH-anhydrous ethanol mixed solution in which NaOH has been completely dissolved into DHA oil to react, removing ethanol and water in the reaction product to obtain fatty acid ethyl ester; molecular distillation of the fatty acid ethyl ester to obtain DHA ethyl ester; (3) immobilized lipase transesterification reaction: mixing DHA ethyl ester with hydrolyzed phospholipid, and then adding into an immobilized enzyme column filled with immobilized lipase to react; (4) adding the reaction product obtained in step (3) into a winterization tank to winterize, and filtering to obtain phospholipid type DHA in crystalline state; (5) molecular distillation of the phospholipid type DHA in crystalline state obtained in step (4) after warming, and the heavy phase is the phospholipid type DHA. The method for preparing phospholipid type DHA is beneficial to improving the content of DHA in the phospholipid type DHA product, and can increase the oxidation stability of the phospholipid type DHA through microcapsule embedding, and prolong the shelf life.

Disclosed herein are methods of processing an oil composition. The oil composition comprises: 40-100 wt% oil, 0-20 wt% ethanol and 0-25 wt% water. The oil comprises 95-99 wt% triacylglycerides, 1-5 wt% diacylglycerides, and less than 1 wt% monoacylglycerides. The methods disclosed herein may comprise adding water and a base to the oil composition or adding water and an enzyme to the oil composition. Such methods comprise hydrolysing at least some of the diacylglycerides and / or triacylglycerides to obtain an oil comprising at least 10 wt% monoacylglycerides and / or at least 10 wt% diacylglycerides. The methods disclosed herein may comprise adding ethanol and a base to the oil composition. Such methods comprise transesterification of at least some of the monoacylglycerides, diacylglycerides and / or triacylglycerides to obtain an oil comprising at least 20 wt% fatty acid ethyl esters (FAEE). Also provided herein are products obtainable by the disclosed methods.

The invention discloses a coding gene of cutinase CutBJB6 for synthesizing a plurality of fatty acid ethyl esters from a Lacnella arida source and an application of the coding gene of cutinase CutBJB6. The amino acid sequence of the cutinase CutBJB6 is as shown in SEQ ID NO. 1, and the nucleotide sequence of the coding gene of the cutinase CutBJB6 is as shown in SEQ ID NO. 2. The invention belongs to the technical field of biosynthesis, and particularly discloses a coding gene of the Lacnellada-derived cutinase CutBJB6 and construction of an escherichia coli expression vector carrying the gene, and also discloses application of the Lacnellada-derived cutinase CutBJB6 in catalytic synthesis of ethyl butyrate, ethyl valerate, ethyl hexanoate, ethyl caprylate and ethyl caprate in a water-phase system. The invention further discloses a preparation method of the Lacnellada-derived cutinase CutBJB6 and application of the Lacnellada-derived cutinase CutBJB6 in the field of biosynthesis of the Lacnellada-derived cutinase CutBJB6. The invention firstly provides the cutinase CutBJB6 which has the capability of synthesizing fatty acid ethyl ester in a water phase system and is derived from the Lacnella arida and the coding gene of the cutinase CutBJB6.

The invention discloses a detection method and application of fatty acid methyl ester and fatty acid ethyl ester in biological fuel oil, the detection method comprises the following steps: S1, dissolving the biological fuel oil with a solvent to obtain a solution, carrying out solid-phase extraction, and collecting an eluent as a solution to be detected; and S2, carrying out gas chromatography-mass spectrometry analysis on the solution to be detected in the step S1 by using an external standard method and adopting a direct sample injection mode, and calculating to obtain the contents of fatty acid methyl ester and fatty acid ethyl ester in the biological fuel oil. According to the method, the biological fuel oil is dissolved by adopting a specific solvent, then solid-phase extraction is carried out by adopting a specific solid-phase extraction column, a specific eluting agent and a specific eluent, and analysis is carried out by utilizing gas chromatography-mass spectrometry of a specific gas chromatography stationary phase. Qualitative and quantitative detection of the fatty acid methyl ester and the fatty acid ethyl ester in the biological fuel oil is achieved, and the method is high in sensitivity, accuracy, precision and reproducibility.

Compositions and methods for improving one or more agronomic traits of a plant are provided, which include the use of one or more fatty acids, including fatty acid methyl esters and fatty acid ethyl esters, that kill or inhibit the growth of cells in the shoot apical meristem. Use of the composition between vegetative growth stage 1 (VI ) and vegetative growth stage 6 (V6) of the plant is described.

Methods of using silica-zirconia catalysts in processes to reduce an amount of glycidol, glycidyl ester(s), or both glycidol and glycidyl ester(s) from a fatty acid ethyl ester-containing composition are disclosed. The fatty acids are preferably omega-3 or omega-6 fatty acids such as ERA and DHA. Silica-zirconia catalysts and methods of making silica-zirconia catalysts are also disclosed.

This invention discloses a method for preparing krill oil with high phospholipid content, comprising the following steps: 1) mixing Antarctic krill oil with 90% ethanol, adding lipase B to react, enzymatically hydrolyzing the triglycerides in the Antarctic krill oil to release free fatty acids, which then combine in situ with ethanol to form fatty acid ethyl esters; 2) removing the solvent by rotary evaporation under reduced pressure; 3) removing the fatty acid ethyl esters by molecular distillation. Through this method, an Antarctic krill oil concentrate with a phospholipid content of over 90% is obtained. The entire process effectively avoids the damage to phospholipids and active ingredients caused by high temperature and oxidation. This invention does not use potentially harmful organic substances in the preparation process, ensuring the product's safety and reliability, and its applicability in food or pharmaceutical preparation. The preparation process of this invention is simple and suitable for large-scale industrial application.

The present invention provides a fish oil-based amino acid surfactant and a preparation method thereof. Sodium amino acid, sodium hydroxide, and an ethanol-soft water mixture are added into an autoclave and fully dissolved; fish oil fatty acid ethyl ester is added for amidation reaction; after the segmented heat preservation reaction is completed, it is cooled; the acylation reaction mixture is vacuum filtered, the filter cake is carefully rinsed with cold ethanol, and vacuum dried to obtain a fish oil acyl glycine sodium bio-based surfactant. The present invention uses the fish oil fatty acid ethyl ester remaining after enriching eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in polyunsaturated fatty acids ethyl ester as the raw material of the bio-based surfactant after special stabilization and deodorization treatment. The separation is simple and the process flow is relatively short; the synthesized fish oil-based amino acid biosurfactant has good detergency, foaming, emulsifying, and dispersing abilities.

This invention discloses a method for preparing fermented small-molecule plant oils and their application in cosmetics. The preparation method includes: using various plant oils such as camellia seed oil, grape seed oil, olive oil, milk thistle seed oil, peony seed oil, sunflower seed oil, European hazelnut seed oil, corn germ oil, sweet almond oil, and avocado oil as raw materials, and using specific enzymatic fermentation technology under low-temperature fermentation conditions to convert triglycerides in the oils into fatty acid monoesters, and further obtaining fatty acid ethyl esters through enzymatic catalytic esterification; then combining molecular distillation technology to separate and remove high-viscosity components, finally obtaining small-molecule oils with significantly reduced molecular weight and a structure mainly composed of monoesters.

The invention provides a synthesis process of triglyceride type fish oil and the triglyceride type fish oil, and particularly relates to the technical field of fish oil preparation. The synthesis process of the triglyceride type fish oil comprises the following steps: A, adding ethyl ester type fish oil, glycerol and a catalyst into a reaction container for reaction; b, cooling after the reaction is completed, adding a reaction stopping solution, standing and separating to obtain oil liquid; and C, carrying out molecular distillation and refining on the oil liquid to obtain the triglyceride type fish oil. According to the synthesis process provided by the invention, efficient transesterification reaction is realized, fatty acid ethyl ester and glycerol form fatty acid triglyceride in the transesterification process, the occurrence of excessive reaction and side reaction is prevented, and the purity and bioavailability of the final product are ensured. The whole synthesis process not only simplifies the operation and reduces the production cost, but also improves the safety and environmental friendliness of the product, and ensures the nutritional value and health benefits of the triglyceride type fish oil, so that the triglyceride type fish oil becomes a high-value healthy food raw material.

The invention discloses a high-yield medium-chain fatty acid ethyl ester yeast strain XN-G35 and application thereof in wine brewing, the yeast strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.39006, the preservation date is October 15, 2025, the preservation number is CGMCC No.39006, and the preservation number is CGMCC No.39006. The preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The yeast strain disclosed by the invention has the following core advantages: 1, the strain is high in environmental tolerance and good in industrial adaptability; 2, the synergistic aroma enhancement effect is obvious, and the fruity flavor of the grape wine is greatly improved; 3, unique composite fragrance is created, and flavor homogenization is broken; and 4, the process compatibility is good, and the quality of the wine body can be optimized.

The invention discloses a multi-effect fish oil capsule for first-class prevention of heart and cerebral vessels and a preparation process of the multi-effect fish oil capsule. The capsule is prepared from high-purity ocean-derived Omega-3 fatty acid ethyl ester, coenzyme Q10 and vitamin E in a specific proportion. A unique low-temperature emulsification-double-layer embedding process is adopted: firstly, raw materials are pre-emulsified to form stable micro-emulsion, then double-layer protection particles are formed through complex coacervation embedding and enteric coating, and finally the particles are pressed into pills. Through the synergistic effect of the active components, the pharmaceutical composition shows remarkable multiple prevention effects in the aspects of regulating blood fat, resisting inflammation, resisting oxidation and improving the vascular endothelial function, the process ensures the long-term stability and bioavailability of the components, and an innovative nutrition intervention scheme is provided for first-level prevention of cardiovascular and cerebrovascular diseases.

The invention relates to an EPA (Eicosapentaenoic Acid) ethyl ester purification method based on combined application of lipase catalytic ester exchange and high-vacuum rectification, and belongs to the field of fish oil refinement. C20-C22 saturated and monounsaturated fatty acid ethyl ester or polyunsaturated fatty acid ethyl ester with small steric hindrance and alcohol with specific molecular weight are preferentially subjected to grafting reaction, and the molecular weight is changed, so that the boiling point is changed; according to the method disclosed by the invention, the reaction product is further separated and enriched in combination with a high-vacuum rectification technology, the problem that the EPA ethyl ester is difficult to separate due to similar physicochemical properties with other fatty acid ethyl esters in a traditional rectification method is successfully solved, the purity of the EPA ethyl ester is greatly improved, the final purity reaches 85% or above, and the content of DHA ethyl ester is controlled to be 5% or below.

The invention discloses pichia kudriavzevii XJ-YPPKU067-1 with ethanol tolerance, high yield of ethanol and fatty acid ethyl ester and low yield of isoamyl alcohol and application of the pichia kudriavzevii XJ-YPPKU067-1 in production of rosa sterilis fruit wine, and belongs to the technical field of microbial fermentation. The pichia kudriavzevii XJ-YPPKU067-1 disclosed by the invention is separated and screened from Maotai-flavor liquor high-temperature yeast, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31175. The invention further discloses a preparation method of the pichia kudriavzevii XJ-YPPKU067-1. The strain can grow in an environment with ethanol volume percentage concentration of 15%, and has the advantages of high yield of ethanol and a variety of fatty acid ethyl esters and no production of isoamyl alcohol. When the pichia kudriavzevii XJ-YPPKU067-1 is applied to preparation of the fruit wine, the alcohol content of the fruit wine and the content of fatty acid ethyl ester can be improved, the content of harmful isoamyl alcohol is reduced, and then the quality of the fruit wine is improved.

In the present invention a new synthesis method for fatty acid methyl ester and fatty acid ethyl ester is provided. More specifically, a fatty oil composition obtained from dried insects by mechanical treatment is reacted with aliphatic alcohols in the presence of alkali metal alkoxide salts such as methoxide or ethoxide to obtain fatty acid methyl ester (FAME) or fatty acid ethyl ester (FAEE).