19 results about "Fatty acids.ethyl esters" patented technology

The invention discloses a preparation method of omega-3 fatty acid ethyl ester 90 soft capsules, which comprises the following steps: 1) preparing raw materials and auxiliary materials: weighing gelatin, glycerol, purified water and omega-3 fatty acid ethyl ester 90 according to prescription dosage for later use; (2) preparing a glue solution, namely heating purified water to 80 DEG C, sequentially adding glycerol and gelatin for capsules, stirring until the gelatin for capsules is melted, carrying out vacuum stirring and degassing for more than 30 minutes, and standing and degassing for 30-60 minutes; 3) pelleting: adjusting the temperature of a capsule box, the temperature of a spraying body, the rotating speed of a main shaft and the refrigeration temperature of a roller, adjusting the thickness of a wet capsule skin, adding the omega-3 fatty acid ethyl ester 90 prepared in the step 1) into a hopper, and starting pelleting; and 4) drying: controlling the temperature of a drying chamber at 10-30 DEG C, controlling the humidity below 40%, and drying until the water content of the capsule skin is less than 13% to obtain the omega-3 fatty acid ethyl ester 90 soft capsule, the process is simple, and the prepared omega-3 fatty acid ethyl ester 90 soft capsule has good disintegration effect and stable performance.

The present application belongs to the field of food oil, and particularly relates to a method for processing olive oil, especially virgin olive oil. The method comprises the following steps: cleaning, drying and crushing olive pomace after oil extraction by using an organic solvent (n-hexane or ethanol), adding a certain amount of olive pomace powder into olive oil and stirring under vacuum, then removing the precipitate, and finally processing under ultrahigh pressure. The content of ethyl ester of fatty acid in the virgin olive oil processed by the method of the present application can be kept less than or equal to 35 mg / kg under the condition of 30 DEG C and packaging in the dark for 12-24 months.

The invention discloses a collophanite flotation combined collecting agent and a preparation method and application thereof. The phosphorite flotation combined collecting agent comprises agent components and water with the same mass, and the agent components are composed of, by mass, 40%-85% of mixed amine, 8%-30% of a mixed nonionic foaming agent and 7%-30% of a hydrophobic accelerant; the mixed amine is a mixture of N-dodecyl ethanolamine and alkoxylated polyethyleneimine; the mixed nonionic foaming agent is a mixture of methyl isobutyl carbinol and C5-C9 fatty acid ethyl ester; the hydrophobic accelerant is a mixture of polymethylhydrosiloxane and kerosene. The components of the collecting agent are optimized, the synergistic effect of all the components is exerted, the using amount of the collecting agent in the reverse flotation desilicication process is reduced, the production cost is reduced, and the flotation froth suitability and the flotation effect are improved.

The invention belongs to the field of wine brewing, and relates to a rosa sterilis and crystal grape mixed fruit wine rich in fatty acid ethyl ester and a preparation method of the rosa sterilis and crystal grape mixed fruit wine. According to the preparation method, the rosa sterilis and crystal grape mixed fruit juice is taken as a main raw material, fermentation is carried out by virtue of Saccharomyces cerevisiae XJ-YSSCE010-1, flavor substances such as ethanol and fatty acid ethyl ester are generated, and the rosa sterilis and crystal grape mixed fruit wine rich in fatty acid ethyl ester is prepared. The saccharomyces cerevisiae XJ-YSSCE010-1 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the saccharomyces cerevisiae XJ-YSSCE010-1 is CGMCC (China General Microbiological Culture Collection Center) No. 31174. The preparation method disclosed by the invention is beneficial to increasing the content of flavor substances such as fatty acid ethyl ester in the rosa sterilis and crystal grape mixed fruit wine.

The invention discloses an esterification reaction system and a cyclic esterification reaction method. The system comprises a batching kettle, the batching kettle passes through a glycerol feeding end, a high-pressure nitrogen feeding end and a fatty acid ethyl ester feeding end, a reaction tank, a stirring shaft, a fan-shaped stirring paddle and a stirring pipe arranged close to the inner wall are arranged in the batching kettle, a first filter plate and a vertical second filter plate are arranged at the bottom of the batching kettle and used for separating enzyme from a product, and high-pressure nitrogen can be introduced into a nitrogen nozzle at the bottom of the batching kettle. The fatty acid ethyl ester feeding end is communicated with the pretreatment kettle and the ethanol tank, so that the esterification pretreatment of high-acid-value fatty acid is realized; the top of the batching kettle is communicated with a condenser through a vacuum pipeline and a vacuum pump, and ethanol and redundant nitrogen generated in the reaction are extracted and condensed and then are respectively recovered to an ethanol tank and a nitrogen tank for cyclic utilization. The method comprises the following steps: continuously adding raw materials according to set steps, pressurizing nitrogen, heating and stirring, vacuumizing and condensing, recycling ethanol and nitrogen, separating enzyme through two-stage filtration, and refluxing unreacted materials until the high-quality triglyceride type fish oil is obtained.

The invention discloses a hydrolase E632 coding gene for synthesizing a plurality of fatty acid ethyl esters from Azoarcus indigens and an application of the hydrolase E632 coding gene. The amino acid sequence of the hydrolase E632 is as shown in SEQ ID NO.1, and the nucleotide sequence of the coding gene of the hydrolase E632 is as shown in SEQ ID NO.2. The invention belongs to the technical field of hydrolase engineering, particularly discloses construction of an Azoarcus indigens source hydrolase E632 coding gene and an escherichia coli expression vector carrying the gene, and further discloses application of the Azoarcus indigens source hydrolase E632 in catalytic synthesis of ethyl valerate, ethyl hexanoate, ethyl caprylate and ethyl caprate in a water phase system. The invention firstly provides a hydrolase E632 which is derived from Azoarcus indigens and has the capability of catalytically synthesizing fatty acid ethyl ester in a water phase system and a coding gene of the hydrolase E632.

A method for preparing phospholipid type DHA, comprising the following steps: (1) hydrolysis of phospholipid to obtain hydrolyzed phospholipid; (2) preparation of DHA ethyl ester: slowly adding NaOH-anhydrous ethanol mixed solution in which NaOH has been completely dissolved into DHA oil to react, removing ethanol and water in the reaction product to obtain fatty acid ethyl ester; molecular distillation of the fatty acid ethyl ester to obtain DHA ethyl ester; (3) immobilized lipase transesterification reaction: mixing DHA ethyl ester with hydrolyzed phospholipid, and then adding into an immobilized enzyme column filled with immobilized lipase to react; (4) adding the reaction product obtained in step (3) into a winterization tank to winterize, and filtering to obtain phospholipid type DHA in crystalline state; (5) molecular distillation of the phospholipid type DHA in crystalline state obtained in step (4) after warming, and the heavy phase is the phospholipid type DHA. The method for preparing phospholipid type DHA is beneficial to improving the content of DHA in the phospholipid type DHA product, and can increase the oxidation stability of the phospholipid type DHA through microcapsule embedding, and prolong the shelf life.

The invention discloses a coding gene of cutinase CutBJB6 for synthesizing a plurality of fatty acid ethyl esters from a Lacnella arida source and an application of the coding gene of cutinase CutBJB6. The amino acid sequence of the cutinase CutBJB6 is as shown in SEQ ID NO. 1, and the nucleotide sequence of the coding gene of the cutinase CutBJB6 is as shown in SEQ ID NO. 2. The invention belongs to the technical field of biosynthesis, and particularly discloses a coding gene of the Lacnellada-derived cutinase CutBJB6 and construction of an escherichia coli expression vector carrying the gene, and also discloses application of the Lacnellada-derived cutinase CutBJB6 in catalytic synthesis of ethyl butyrate, ethyl valerate, ethyl hexanoate, ethyl caprylate and ethyl caprate in a water-phase system. The invention further discloses a preparation method of the Lacnellada-derived cutinase CutBJB6 and application of the Lacnellada-derived cutinase CutBJB6 in the field of biosynthesis of the Lacnellada-derived cutinase CutBJB6. The invention firstly provides the cutinase CutBJB6 which has the capability of synthesizing fatty acid ethyl ester in a water phase system and is derived from the Lacnella arida and the coding gene of the cutinase CutBJB6.

The invention discloses a detection method and application of fatty acid methyl ester and fatty acid ethyl ester in biological fuel oil, the detection method comprises the following steps: S1, dissolving the biological fuel oil with a solvent to obtain a solution, carrying out solid-phase extraction, and collecting an eluent as a solution to be detected; and S2, carrying out gas chromatography-mass spectrometry analysis on the solution to be detected in the step S1 by using an external standard method and adopting a direct sample injection mode, and calculating to obtain the contents of fatty acid methyl ester and fatty acid ethyl ester in the biological fuel oil. According to the method, the biological fuel oil is dissolved by adopting a specific solvent, then solid-phase extraction is carried out by adopting a specific solid-phase extraction column, a specific eluting agent and a specific eluent, and analysis is carried out by utilizing gas chromatography-mass spectrometry of a specific gas chromatography stationary phase. Qualitative and quantitative detection of the fatty acid methyl ester and the fatty acid ethyl ester in the biological fuel oil is achieved, and the method is high in sensitivity, accuracy, precision and reproducibility.

Methods of using silica-zirconia catalysts in processes to reduce an amount of glycidol, glycidyl ester(s), or both glycidol and glycidyl ester(s) from a fatty acid ethyl ester-containing composition are disclosed. The fatty acids are preferably omega-3 or omega-6 fatty acids such as ERA and DHA. Silica-zirconia catalysts and methods of making silica-zirconia catalysts are also disclosed.

This invention discloses a method for preparing krill oil with high phospholipid content, comprising the following steps: 1) mixing Antarctic krill oil with 90% ethanol, adding lipase B to react, enzymatically hydrolyzing the triglycerides in the Antarctic krill oil to release free fatty acids, which then combine in situ with ethanol to form fatty acid ethyl esters; 2) removing the solvent by rotary evaporation under reduced pressure; 3) removing the fatty acid ethyl esters by molecular distillation. Through this method, an Antarctic krill oil concentrate with a phospholipid content of over 90% is obtained. The entire process effectively avoids the damage to phospholipids and active ingredients caused by high temperature and oxidation. This invention does not use potentially harmful organic substances in the preparation process, ensuring the product's safety and reliability, and its applicability in food or pharmaceutical preparation. The preparation process of this invention is simple and suitable for large-scale industrial application.

This invention discloses a method for preparing fermented small-molecule plant oils and their application in cosmetics. The preparation method includes: using various plant oils such as camellia seed oil, grape seed oil, olive oil, milk thistle seed oil, peony seed oil, sunflower seed oil, European hazelnut seed oil, corn germ oil, sweet almond oil, and avocado oil as raw materials, and using specific enzymatic fermentation technology under low-temperature fermentation conditions to convert triglycerides in the oils into fatty acid monoesters, and further obtaining fatty acid ethyl esters through enzymatic catalytic esterification; then combining molecular distillation technology to separate and remove high-viscosity components, finally obtaining small-molecule oils with significantly reduced molecular weight and a structure mainly composed of monoesters.

The invention discloses a high-yield medium-chain fatty acid ethyl ester yeast strain XN-G35 and application thereof in wine brewing, the yeast strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.39006, the preservation date is October 15, 2025, the preservation number is CGMCC No.39006, and the preservation number is CGMCC No.39006. The preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The yeast strain disclosed by the invention has the following core advantages: 1, the strain is high in environmental tolerance and good in industrial adaptability; 2, the synergistic aroma enhancement effect is obvious, and the fruity flavor of the grape wine is greatly improved; 3, unique composite fragrance is created, and flavor homogenization is broken; and 4, the process compatibility is good, and the quality of the wine body can be optimized.

The invention discloses a multi-effect fish oil capsule for first-class prevention of heart and cerebral vessels and a preparation process of the multi-effect fish oil capsule. The capsule is prepared from high-purity ocean-derived Omega-3 fatty acid ethyl ester, coenzyme Q10 and vitamin E in a specific proportion. A unique low-temperature emulsification-double-layer embedding process is adopted: firstly, raw materials are pre-emulsified to form stable micro-emulsion, then double-layer protection particles are formed through complex coacervation embedding and enteric coating, and finally the particles are pressed into pills. Through the synergistic effect of the active components, the pharmaceutical composition shows remarkable multiple prevention effects in the aspects of regulating blood fat, resisting inflammation, resisting oxidation and improving the vascular endothelial function, the process ensures the long-term stability and bioavailability of the components, and an innovative nutrition intervention scheme is provided for first-level prevention of cardiovascular and cerebrovascular diseases.

The invention discloses pichia kudriavzevii XJ-YPPKU067-1 with ethanol tolerance, high yield of ethanol and fatty acid ethyl ester and low yield of isoamyl alcohol and application of the pichia kudriavzevii XJ-YPPKU067-1 in production of rosa sterilis fruit wine, and belongs to the technical field of microbial fermentation. The pichia kudriavzevii XJ-YPPKU067-1 disclosed by the invention is separated and screened from Maotai-flavor liquor high-temperature yeast, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31175. The invention further discloses a preparation method of the pichia kudriavzevii XJ-YPPKU067-1. The strain can grow in an environment with ethanol volume percentage concentration of 15%, and has the advantages of high yield of ethanol and a variety of fatty acid ethyl esters and no production of isoamyl alcohol. When the pichia kudriavzevii XJ-YPPKU067-1 is applied to preparation of the fruit wine, the alcohol content of the fruit wine and the content of fatty acid ethyl ester can be improved, the content of harmful isoamyl alcohol is reduced, and then the quality of the fruit wine is improved.

The invention provides a method for preparing an astaxanthin isomer compound with high oil solubility through alcoholysis of astaxanthin oleoresin. The method comprises the following steps: by taking astaxanthin oleoresin as a raw material and ethanol as a reaction medium, carrying out alcoholysis treatment on astaxanthin ester mainly comprising trans-configuration under the action of an ethoxide catalyst, so that the astaxanthin ester is converted into free astaxanthin containing cis-configuration; converting and removing the catalyst, and removing ethanol in the system through negative pressure distillation to obtain a mixture mainly composed of astaxanthin isomers and fatty acid ethyl ester. According to the method, not only can the oil solubility and bioavailability of the astaxanthin be remarkably improved, but also the high astaxanthin retention rate can be kept in the preparation process. The whole reaction system is green and environment-friendly, the food safety of the product can be fully guaranteed, and the method has the advantages of being simple, efficient, energy-saving and easy to implement on a large scale, is beneficial to enhancing functional application of astaxanthin in the fields of health food and the like, and effectively widens the market application range of astaxanthin.

An estrosphere preparation comprising an ester phase and an aqueous phase characterised in that the ester phase comprises α-linolenic acid ethyl ester, or a mixture of α-linolenic acid ethyl ester with one or more components selected from the group comprising polyunsaturated fatty acids ethyl esters (PUFA ethyl esters) or triglycerides, ethanol, and emulsifier, in a weight ratio of between 2:0.5:1 and 8:2:1, respectively, and the aqueous phase comprises water with glycerol at a final concentration of 2.25% by weight, wherein the weight ratio of α-linolenic acid ethyl ester, or of the mixture of α-linolenic acid ethyl ester with one or more components to ethanol, emulsifier and water is between 2:0.5:1:45 and 8:2:1:45, respectively. A method for producing such an estrosphere preparation. Said preparation for use in the treatment of cancer and viral diseases.

The invention discloses a hydrolase DAH8 coding gene for synthesizing various fatty acid ethyl esters from a Burkholderia seminis source and an application of the hydrolase DAH8 coding gene. The amino acid sequence of the hydrolase DAH8 is as shown in SEQ ID NO.1, and the nucleotide sequence of the coding gene of the hydrolase DAH8 is as shown in SEQ ID NO.2. The invention belongs to the technical field of hydrolase and particularly discloses construction of a Burkholderia seminalis source hydrolase DAH8 encoding gene and an escherichia coli expression vector carrying the gene, and meanwhile, the invention further discloses application of the Burkholderia seminalis source hydrolase DAH8 to catalytic synthesis of ethyl butyrate, ethyl valerate, ethyl hexanoate, ethyl caprylate and ethyl caprate in a water phase system. The invention firstly provides a hydrolase DAH8 which has the capability of synthesizing fatty acid ethyl ester in a water phase system and is derived from Burkholderia seminalis and a coding gene of the hydrolase DAH8.

The invention discloses a method for separating DHA and EPA in fatty acid or separating DHA-EE and DPA-EE in fatty acid ethyl ester. DHA is separated from a DHA and EPA mixed solution through affinity difference between FABP protein and fatty acid, and the problems of high energy consumption and easy oxidation of a traditional separation method are avoided. The preparation method comprises the following steps: firstly, respectively adding strep-tag II and six histidine tags to N ends and C ends of FABP3 and FABP7 protein sequences, inserting the FABP3 and FABP7 protein sequences into a plasmid pET-28a (+), and expressing FABP3 / FABP7 protein with tags through escherichia coli; then, the affinity (Kd) of FABP3 and FABP7 with DHA / EPA is measured through a BLI experiment, and the affinity (Kd) is expanded to the affinity (Kd) of DHA-EE / DPA-EE; finally, the protein is coupled to a nickel column, and DHA / EPA is subjected to quantitative analysis through GC-MS to evaluate the protein separation effect.