31 results about "Burkholderia seminalis" patented technology

The invention discloses Burkholderia cepacia and an application thereof. A bacterial strain is Burkholderia cepacia pt6, and a preservation number of the bacterial strain is CGMCC No. 7598 (China General Microbiological Culture Collection Center Number 7598). The invention further discloses a microorganism bacterium agent containing the bacterial strain and a preparation method. An experiment indicates that fermentation liquor of the Burkholderia cepacia pt6 contains a large number of phosphate solubilizing bacteria; the phosphate solubilizing bacteria have definite positive effects on soil improvement; and phosphate solubilizing functions of the phosphate solubilizing bacteria have important significance in increasing a use ratio of a phosphate fertilizer, increasing a yield of crop and improving the quality of the crop.

The invention discloses pseudomonas aeruginosa bacteriophage endolysins, and a coding gene and application thereof. The invention provides a protein which is as shown in (a) or (b) as below: (a) the protein which is as shown in a sequence 1 in a sequence table and consists of an amino acid sequence; (b) the protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence which is as shown in the sequence 1 in the sequence table, has a function of cracking gram-negative bacteria and is deviated from the sequence 1 in the sequencetable. The protein provided by the invention can crack a plurality of gram-negative bacteria, such as Escherichia coli, Klebsiella pneumonia, pseudomonas aeruginosa, Burkholderia cepacia and Salmonella typhimurium. The protein and a surfactant EDTA (Ethylene Diamine Tetraacetic Acid) act synergistically to achieve a stronger inhibiting effect on the gram-negative bacteria. A foundation is laid for researching and developing a new antibacterial preparation.

A novel predator bacterium Burkholderia casidae is disclosed. The invention is directed to the isolation and use of Burkholderia casidae to control microbial diseases of plants. The genetic, biochemical and physiological characteristics of Burkholderia casidae are described. Biocontrol compositions comprising Burkholderia casidae, and antimicrobial compounds and antimicrobial preparations prepared from Burkholderia casidae are also disclosed, as are methods for accomplishing all of the foregoing.

A novel predator bacterium Burkholderia casidae is disclosed. The invention is directed to the isolation and use of Burkholderia casidae to control microbial diseases of plants. The genetic, biochemical and physiological characteristics of Burkholderia casidae are described. Biocontrol compositions comprising Burkholderia casidae, and antimicrobial compounds and antimicrobial preparations prepared from Burkholderia casidae are also disclosed, as are methods for accomplishing all of the foregoing.

The invention provides Burkholderia and application, and belongs to the technical field of microbial strains. The Burkholderia XYQH-1 is preserved in the CCTCC (China Center for Type Culture Collection), and the preservation number is CCTCC M 2021105. After a culture solution of the strain is inoculated to rhizosphere soil of root parts of potted tea seedlings, the growth of the tea seedlings can be promoted, the total amount of tea polyphenols is increased, and the fermentation conditions of the Burkholderia XYQH-1 are easy to operate. Meanwhile, the Burkholderia XYQH-1 can also be used for preparing a natural microbial fertilizer, can promote the growth of tea trees and increase the content of tea polyphenols, provides an alternative for the tea market, belongs to a novel environment-friendly fertilizer, and can greatly reduce the environmental pollution caused by chemical fertilizers.

The invention provides a Burkholderia bacterium and its application, belonging to the technical field of microbial strains. The Burkholderia bacterium XYQH-1 is preserved in the China Center for Typical Culture Collection, and the preservation number is: CCTCC M 2021105; The culture solution of the strain is inoculated in the root rhizosphere soil of potted tea seedlings, which can promote the growth of tea seedlings and improve the efficacy of the total amount of tea polyphenols. The fermentation conditions of Burkholderia XYQH-1 of the present invention are easy to operate. At the same time, the Burkholderia XYQH-1 can also prepare natural microbial fertilizers, which can promote the growth of tea trees and increase the content of tea polyphenols, providing an alternative for the tea market and a new type of environmentally friendly fertilizer. Fertilizers can greatly reduce the environmental pollution caused by chemical fertilizers.

The invention discloses a rapid-recognition gene chip for pathogenic bacteria of pneumonia. The gene chip can detect 15 pathogenic bacteria including streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae, and clinically common and difficult-to-culture pathogenic bacteria are contained. In a preparation process of the gene chip, design, screening and verification of probes are performed by adopting 16S rDNA and a specific gene sequence corresponding to each of the pathogenic bacteria, and types of the bacteria in a to-be-detected sample are identified from levels of genus and species simultaneously and respectively. The gene chip has the advantages that the defect that clinical detection of the pathogenic bacteria of pneumonia is not timely and comprehensive at present is overcome, and one novel detection way is provided for early diagnosis and early treatment of patients with pneumonia.

Novel compounds having antimicrobial activity, in particular against Pseudomonas aeruginosa, Burkholderia cepacia and / or Clostridium difficile, and a pharmaceutical composition containing the novel compound.

The invention belongs to the technical field of biological treatment of environmental pollutants, and discloses Burkholderia GYP1 and its application in degrading brominated flame retardants. The Burkholderia cepacia GYP1 was deposited in the China Center for Type Culture Collection (CCTCC) on March 9, 2017, and the preservation number is CCTCC NO: M 2017103. The strain Burkholderia cepacia GYP1 of the present invention degrades 2,2′,4,4′-tetrabromodiphenyl ether with an initial concentration of 1 mg / L at a pH of 4.0 to 7.0, a temperature of 30°C, and 150 r / min shaking culture for 4 days The rate can reach more than 80%. The bacteria can be used for bioremediation of water and soil polluted by polybrominated diphenyl ethers.

The invention relates to a preparation method of a novel biological soil remediation agent. The method comprises the following steps: (1) compounding and mixing four bacteria, including anaerobic spore-bearing bacilli TIBCC1.002, micrococcus luteus TCCC23001, pseudomonas fluorescens AS1.867, Burkholderia cepacia NKCCMRNK.T2-2 according to the weight part ratio of (3-6) to (2-5) to (1-4) to (1-3) to form a compound bacterium solution, adding the compound bacterium solution according to the weight ratio of 1%-5% into active sludge, and biologically enhancing the active sludge; (2) filtering biogas slurry by a hollow fiber membrane with the pore diameter of 0.1-0.2 micron so as to obtain waste biogas slurry residues; (3) mixing the biologically enhanced active sludge with the waste biogas slurry residues according to the weight part ratio of (3-9) to (1-5), and stirring uniformly to form the novel biological soil remediation agent. The invention provides another approach for the recycling of the residual sludge and biogas slurry, so that not only can the polluted soil be remediated effectively, but also the load to the environment pollution by the residual sludge and the waste biogas slurry residues can be relieved to a certain extent; the preparation method has relatively high practical application value.

The invention discloses a strain of Burkholderia latta PN1 and its application. The classification of the strain is named Burkholderialata, the strain number is PN1, and it is preserved in the China Center for Type Culture Collection with the preservation number: CCTCC NO: M 2019880. The bacterial strain PN1 screened from the root system of moso bamboo in the present invention has a significant antibacterial effect on the pathogen of moso bamboo shoot die-back, Phyllostachys coccus, the antibacterial rate of its fermentation broth can reach 58.82%, and it has high-efficiency phosphorus-solubilizing and IAA-secreting abilities; The endophytic bacterium PN1 of moso bamboo of the present invention can significantly promote the growth of moso bamboo seedlings, and test data show that the liquid bacterial agent prepared by the strain PN1 can significantly improve growth indexes such as ground diameter, seedling height and chlorophyll of moso bamboo. Therefore, the present invention provides excellent bacterial strain resources for developing environment-friendly moso bamboo biological special fertilizers.

The invention discloses a Burkholderia cepacia ( Burkholderia cepacia )WY6‑5 and its application, the deposit number is: CCTCC M 2018248. The Burkholderia cepacia ( Burkholderia cepacia )WY6‑5 has good applications in phosphorus degradation, antibacterial and plant growth promotion, and preparation of dimethyl disulfide.

The invention relates to an irrigation method for repairing soil microbial flora in continuous cropping of melon crops, comprising the following steps: (1) preparing Burkholderia biological agent; (2) mixing load particles; (3) preparing irrigation Water and fertilizer, the bacteria concentration of Burkholderia in the irrigation water and fertilizer is 1×10 ‑1 ×10 3 cfu / ml, seaweed fertilizer 0.0001-0.01g / L; (4) Apply irrigation water and fertilizer, the irrigation water and fertilizer adopts the method of hose drip irrigation, and the amount of water and fertilizer irrigation is 7-15 cubic meters per mu. The method uses irrigation water containing repairing probiotics to irrigate crops, continuously repairs continuous cropping soil, reduces the growth rate of phenolic acid substances in crop soil, increases the richness and diversity of microbial populations in the soil, has a good repair effect, and simultaneously promotes crop growth and resistance. Disease, increase the number of female flowers of melon plants.

The present invention relates to N-acetylcysteine for the use in the treatment of bacterial infections caused by a pathogen selected from Burkholderia cepacia complex (BCC) and Stenotrophomonas maltophilia (S. maltophilia) strains.

The invention provides a method for extracting FR901464 from a fermented liquid obtained by fermentation of Burkholderia. The fermentation method includes: adding the macroporous resin to the fermentation liquid, mixing and absorbing, and filtering; soaking and separating the filtered macroporous resin with an organic solvent, and concentrating the separated organic solvent; mixing the concentrated product with n-hexane and ethyl acetate The solvent is dissolved, and after chromatographic separation with silica gel, the eluent is obtained by eluting with ethyl acetate; It is a C18 filler, which is eluted with a liquid phase eluent to obtain an eluate; the eluate is extracted and the organic phase is collected and evaporated to dryness to form a solid powder.

The invention relates to application of cinnamon essential oil in preparation of bacteriostatic drugs, and belongs to the technical field of medicines. The strain is Burkholderia pseudomallei and Burkholderia cepacia, and the strain is Burkholderia pseudomallei. The cinnamon essential oil can effectively inhibit burkholderia pseudomallei and burkholderia cepacia as a special bacteriostatic agent, the cinnamon essential oil is used as the bacteriostatic agent, dependence of human beings on antibiotics is certainly relieved, a certain foundation is laid for research and development of Chinese herbal medicines, and clinical application significance is achieved. The medicine is a clinical treatment medicine with high efficiency, small toxic and side effects and even no toxic and side effects.

The invention discloses a kit for quickly detecting 15 pneumonia pathogenic bacteria. The kit can detect streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, baumanii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae which cover clinically common pneumonia pathogenic bacteria difficult to culture. 16S rDNA and specific gene sequences corresponding to the pneumonia pathogenic bacteria are detected by combining gene chips with multiple asymmetric PCR reactions, and the categories of the bacteria in a to-be-detected sample are identified in genus and species. The kit makes up for the defect that current clinical detection of pneumonia pathogenic bacteria is not in time or comprehensive and a novel detection means for early diagnosis and early treatment of patients suffering from pneumonia is provided.

The invention belongs to the technical field of plant disease biocontrol, and in particular relates to a strain of Burkholderia cepacia XJYC‑2 and its application in preventing and treating litchi frost blight and litchi anthracnose. The Burkholderia cenocepacia was named as Burkholderia cenocepacia (Burkholderia cenocepacia) XJYC-2, and it was preserved in the Guangdong Microbial Culture Collection Center, referred to as GDMCC, address: No. 100 Xianlie Middle Road, Guangzhou City Guangdong Provincial Institute of Microbiology, 5th Floor, Building 59, Institute of Microbiology, the preservation number is GDMCC No: 60699, and the preservation time is June 17, 2019. The present invention also provides a biological preparation based on the aforementioned Burkholderia cepacia. The Burkholderia cenocepacia and its biological agent of the present invention have significant inhibitory effect on both fungi Pythora lychee and Anthracnose lychee, have good biocontrol effect and broad application prospect.

The invention provides a strain of burkholderia tropica zheng probiotics A of which the preservation number is GDMCC NO.60495, and belongs to the technical fields of new strains of burkholderia and application thereof. The strain of burkholderia tropica zheng probiotics A has the advantages that burkholderia tropica can convert ammonia-nitrogen substances into organic nitrates, while the toxicityof ammonia gas to thalli is reduced, the energy in the nitrate conversion process can also be utilized, and ammonia nitrogen pollution in water, air and soil can also be converted, thereby achieving the effects of purifying the air and water and controlling soil hardening and salinization; the generated organic nitrates can be quickly absorbed and utilized by animals or plants after being dissolved in water, thereby being capable of improving the efficiency of absorption of livestock to the nitrogen element in food, and achieving the purpose of increasing the yield; the burkholderia tropica has the effects of degrading heavy metals, inhibiting bacteria and preventing and controlling plant diseases and pests.

The invention provides a strain of Burkholderia neocepacia and its application. The bacteria is identified as Burkholderia neocepacia, named as Burkholderia cenocepacia CJ-P8, the deposit number is CGMCC NO.12119. The Burkholderia neocepacia CJ-P8 disclosed in the invention is screened from the rhizosphere soil of Radix Pseudostellariae through the flat plate confrontation method, and has a strong effect on the soil-borne pathogenic bacteria of Radix heterophylla isolated earlier—Fusarium oxysporum and Fusarium moniliforme Antagonism, and no pathogenicity to other crops in Pseudostellaria heterophylla. Simultaneously, the present invention provides the optimum culture medium for the growth of the antagonistic bacteria. The optimum formula is 1 / 4 LB+1 / 80 MS (without iron salt)+0.1wt.% brown sugar, and the fermented bacterial liquid has obvious antagonistic effect. The antagonistic strain provided by the invention can effectively prevent and control soil-borne diseases of heterophylla, and is pollution-free, ecological and safe, and has good application prospects.

The invention discloses Burkholderia cepacia and an application thereof. A bacterial strain is Burkholderia cepacia pt6, and a preservation number of the bacterial strain is CGMCC No. 7598 (China General Microbiological Culture Collection Center Number 7598). The invention further discloses a microorganism bacterium agent containing the bacterial strain and a preparation method. An experiment indicates that fermentation liquor of the Burkholderia cepacia pt6 contains a large number of phosphate solubilizing bacteria; the phosphate solubilizing bacteria have definite positive effects on soil improvement; and phosphate solubilizing functions of the phosphate solubilizing bacteria have important significance in increasing a use ratio of a phosphate fertilizer, increasing a yield of crop and improving the quality of the crop.