34 results about "Burkholderia seminalis" patented technology

The invention belongs to the technical field of biological treatment of environmental pollutants, and discloses Burkholderia GYP1 and its application in degrading brominated flame retardants. The Burkholderia cepacia GYP1 was deposited in the China Center for Type Culture Collection (CCTCC) on March 9, 2017, and the preservation number is CCTCC NO: M 2017103. The strain Burkholderia cepacia GYP1 of the present invention degrades 2,2′,4,4′-tetrabromodiphenyl ether with an initial concentration of 1 mg / L at a pH of 4.0 to 7.0, a temperature of 30°C, and 150 r / min shaking culture for 4 days The rate can reach more than 80%. The bacteria can be used for bioremediation of water and soil polluted by polybrominated diphenyl ethers.

The invention relates to a preparation method of a novel biological soil remediation agent. The method comprises the following steps: (1) compounding and mixing four bacteria, including anaerobic spore-bearing bacilli TIBCC1.002, micrococcus luteus TCCC23001, pseudomonas fluorescens AS1.867, Burkholderia cepacia NKCCMRNK.T2-2 according to the weight part ratio of (3-6) to (2-5) to (1-4) to (1-3) to form a compound bacterium solution, adding the compound bacterium solution according to the weight ratio of 1%-5% into active sludge, and biologically enhancing the active sludge; (2) filtering biogas slurry by a hollow fiber membrane with the pore diameter of 0.1-0.2 micron so as to obtain waste biogas slurry residues; (3) mixing the biologically enhanced active sludge with the waste biogas slurry residues according to the weight part ratio of (3-9) to (1-5), and stirring uniformly to form the novel biological soil remediation agent. The invention provides another approach for the recycling of the residual sludge and biogas slurry, so that not only can the polluted soil be remediated effectively, but also the load to the environment pollution by the residual sludge and the waste biogas slurry residues can be relieved to a certain extent; the preparation method has relatively high practical application value.

The invention relates to an irrigation method for repairing soil microbial flora in continuous cropping of melon crops, comprising the following steps: (1) preparing Burkholderia biological agent; (2) mixing load particles; (3) preparing irrigation Water and fertilizer, the bacteria concentration of Burkholderia in the irrigation water and fertilizer is 1×10 ‑1 ×10 3 cfu / ml, seaweed fertilizer 0.0001-0.01g / L; (4) Apply irrigation water and fertilizer, the irrigation water and fertilizer adopts the method of hose drip irrigation, and the amount of water and fertilizer irrigation is 7-15 cubic meters per mu. The method uses irrigation water containing repairing probiotics to irrigate crops, continuously repairs continuous cropping soil, reduces the growth rate of phenolic acid substances in crop soil, increases the richness and diversity of microbial populations in the soil, has a good repair effect, and simultaneously promotes crop growth and resistance. Disease, increase the number of female flowers of melon plants.

The invention provides a method for extracting FR901464 from a fermented liquid obtained by fermentation of Burkholderia. The fermentation method includes: adding the macroporous resin to the fermentation liquid, mixing and absorbing, and filtering; soaking and separating the filtered macroporous resin with an organic solvent, and concentrating the separated organic solvent; mixing the concentrated product with n-hexane and ethyl acetate The solvent is dissolved, and after chromatographic separation with silica gel, the eluent is obtained by eluting with ethyl acetate; It is a C18 filler, which is eluted with a liquid phase eluent to obtain an eluate; the eluate is extracted and the organic phase is collected and evaporated to dryness to form a solid powder.