34 results about "Burkholderia seminalis" patented technology

A novel predator bacterium Burkholderia casidae is disclosed. The invention is directed to the isolation and use of Burkholderia casidae to control microbial diseases of plants. The genetic, biochemical and physiological characteristics of Burkholderia casidae are described. Biocontrol compositions comprising Burkholderia casidae, and antimicrobial compounds and antimicrobial preparations prepared from Burkholderia casidae are also disclosed, as are methods for accomplishing all of the foregoing.

The invention provides a Burkholderia bacterium and its application, belonging to the technical field of microbial strains. The Burkholderia bacterium XYQH-1 is preserved in the China Center for Typical Culture Collection, and the preservation number is: CCTCC M 2021105; The culture solution of the strain is inoculated in the root rhizosphere soil of potted tea seedlings, which can promote the growth of tea seedlings and improve the efficacy of the total amount of tea polyphenols. The fermentation conditions of Burkholderia XYQH-1 of the present invention are easy to operate. At the same time, the Burkholderia XYQH-1 can also prepare natural microbial fertilizers, which can promote the growth of tea trees and increase the content of tea polyphenols, providing an alternative for the tea market and a new type of environmentally friendly fertilizer. Fertilizers can greatly reduce the environmental pollution caused by chemical fertilizers.

The invention discloses a rapid-recognition gene chip for pathogenic bacteria of pneumonia. The gene chip can detect 15 pathogenic bacteria including streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae, and clinically common and difficult-to-culture pathogenic bacteria are contained. In a preparation process of the gene chip, design, screening and verification of probes are performed by adopting 16S rDNA and a specific gene sequence corresponding to each of the pathogenic bacteria, and types of the bacteria in a to-be-detected sample are identified from levels of genus and species simultaneously and respectively. The gene chip has the advantages that the defect that clinical detection of the pathogenic bacteria of pneumonia is not timely and comprehensive at present is overcome, and one novel detection way is provided for early diagnosis and early treatment of patients with pneumonia.

The invention belongs to the technical field of biological treatment of environmental pollutants, and discloses Burkholderia GYP1 and its application in degrading brominated flame retardants. The Burkholderia cepacia GYP1 was deposited in the China Center for Type Culture Collection (CCTCC) on March 9, 2017, and the preservation number is CCTCC NO: M 2017103. The strain Burkholderia cepacia GYP1 of the present invention degrades 2,2′,4,4′-tetrabromodiphenyl ether with an initial concentration of 1 mg / L at a pH of 4.0 to 7.0, a temperature of 30°C, and 150 r / min shaking culture for 4 days The rate can reach more than 80%. The bacteria can be used for bioremediation of water and soil polluted by polybrominated diphenyl ethers.

The invention relates to a preparation method of a novel biological soil remediation agent. The method comprises the following steps: (1) compounding and mixing four bacteria, including anaerobic spore-bearing bacilli TIBCC1.002, micrococcus luteus TCCC23001, pseudomonas fluorescens AS1.867, Burkholderia cepacia NKCCMRNK.T2-2 according to the weight part ratio of (3-6) to (2-5) to (1-4) to (1-3) to form a compound bacterium solution, adding the compound bacterium solution according to the weight ratio of 1%-5% into active sludge, and biologically enhancing the active sludge; (2) filtering biogas slurry by a hollow fiber membrane with the pore diameter of 0.1-0.2 micron so as to obtain waste biogas slurry residues; (3) mixing the biologically enhanced active sludge with the waste biogas slurry residues according to the weight part ratio of (3-9) to (1-5), and stirring uniformly to form the novel biological soil remediation agent. The invention provides another approach for the recycling of the residual sludge and biogas slurry, so that not only can the polluted soil be remediated effectively, but also the load to the environment pollution by the residual sludge and the waste biogas slurry residues can be relieved to a certain extent; the preparation method has relatively high practical application value.

The invention relates to an irrigation method for repairing soil microbial flora in continuous cropping of melon crops, comprising the following steps: (1) preparing Burkholderia biological agent; (2) mixing load particles; (3) preparing irrigation Water and fertilizer, the bacteria concentration of Burkholderia in the irrigation water and fertilizer is 1×10 ‑1 ×10 3 cfu / ml, seaweed fertilizer 0.0001-0.01g / L; (4) Apply irrigation water and fertilizer, the irrigation water and fertilizer adopts the method of hose drip irrigation, and the amount of water and fertilizer irrigation is 7-15 cubic meters per mu. The method uses irrigation water containing repairing probiotics to irrigate crops, continuously repairs continuous cropping soil, reduces the growth rate of phenolic acid substances in crop soil, increases the richness and diversity of microbial populations in the soil, has a good repair effect, and simultaneously promotes crop growth and resistance. Disease, increase the number of female flowers of melon plants.

The invention provides a method for extracting FR901464 from a fermented liquid obtained by fermentation of Burkholderia. The fermentation method includes: adding the macroporous resin to the fermentation liquid, mixing and absorbing, and filtering; soaking and separating the filtered macroporous resin with an organic solvent, and concentrating the separated organic solvent; mixing the concentrated product with n-hexane and ethyl acetate The solvent is dissolved, and after chromatographic separation with silica gel, the eluent is obtained by eluting with ethyl acetate; It is a C18 filler, which is eluted with a liquid phase eluent to obtain an eluate; the eluate is extracted and the organic phase is collected and evaporated to dryness to form a solid powder.

The invention discloses a kit for quickly detecting 15 pneumonia pathogenic bacteria. The kit can detect streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, baumanii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae which cover clinically common pneumonia pathogenic bacteria difficult to culture. 16S rDNA and specific gene sequences corresponding to the pneumonia pathogenic bacteria are detected by combining gene chips with multiple asymmetric PCR reactions, and the categories of the bacteria in a to-be-detected sample are identified in genus and species. The kit makes up for the defect that current clinical detection of pneumonia pathogenic bacteria is not in time or comprehensive and a novel detection means for early diagnosis and early treatment of patients suffering from pneumonia is provided.

The invention belongs to the technical field of plant disease biocontrol, and in particular relates to a strain of Burkholderia cepacia XJYC‑2 and its application in preventing and treating litchi frost blight and litchi anthracnose. The Burkholderia cenocepacia was named as Burkholderia cenocepacia (Burkholderia cenocepacia) XJYC-2, and it was preserved in the Guangdong Microbial Culture Collection Center, referred to as GDMCC, address: No. 100 Xianlie Middle Road, Guangzhou City Guangdong Provincial Institute of Microbiology, 5th Floor, Building 59, Institute of Microbiology, the preservation number is GDMCC No: 60699, and the preservation time is June 17, 2019. The present invention also provides a biological preparation based on the aforementioned Burkholderia cepacia. The Burkholderia cenocepacia and its biological agent of the present invention have significant inhibitory effect on both fungi Pythora lychee and Anthracnose lychee, have good biocontrol effect and broad application prospect.