A strain of Burkholderia tropicalis and its application
A Burkholderia, ammonia nitrogen technology, applied in the application, bacteria, fungicides and other directions, can solve the problems of inability to use nitrogen conversion, low nitrogen conversion efficiency, environmental pollution, etc., and achieve the effect of increasing absorption efficiency
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Embodiment 1
[0043] The preparation of embodiment 1 Burkholderia tropicalis bacterial agent
[0044] Strain: Tropical Burkholder, preservation number: GDMCC NO.60495;
[0045] Medium: 3.0g of beef extract, 10.0g of peptone, 5g of NaCl, 18g of agar, add water to the volume to 1L, adjust the pH to 7.2, and sterilize to obtain the bacterial solution;
[0046] 1. Preparation of liquid bacterial agent
[0047] Inoculate the tropical Burkholder in the liquid culture medium, cultivate it at 25°C for 26 hours, and obtain the bacterial liquid, that is, the concentration is 2×10 8 cfu / g liquid bacterial agent.
[0048] 2. Preparation of solid bacterial agent
[0049] Add maltodextrin (according to the mass fraction of maltodextrin and account for 50%~70% content of total mass to add) in the above-mentioned liquid bacterial preparation, conventional granulation, obtain bacterial content about 1 * 10 8 cfu / g of solid bacteria.
[0050] 3. Spray preparation
[0051] Dilute the bacterial solution ...
Embodiment 2
[0052] Application of embodiment 2 Burkholderia tropicalis bacterial agent in animal husbandry
[0053] 1. Air purification in pig farms
[0054] Application site: a pig farm, the environment of the site is odorous, and the ambient temperature is 24-32°C
[0055] Bacteria agent: the spray agent prepared in Example 1, the concentration of bacteria liquid is 2.4×10 7 cfu / mL;
[0056] Application process: according to 90cfu / cm 2 Spray the bacteria agent on the ground of the farm, and the ammonia smell and the stench of pig manure will be basically eliminated after 2 hours.
[0057] 2. Air purification for cattle breeding sites
[0058] Application site: a cattle farm, the site has a strong smell, and the ambient temperature is 20-26°C;
[0059] Bacteria agent: the spray agent prepared in Example 1, the concentration of bacteria liquid is 2.4×10 7 cfu / mL;
[0060] Application process: according to 75cfu / cm 2 Spray the bacteria agent on the ground of the farm, and the bad s...
Embodiment 3
[0080] Application of embodiment 3 Burkholderia tropicalis bacterial agent in fishery
[0081] 1. Restoration of pond bottom
[0082] In a fish pond, the bottom of the pond is salinized, the water is crystal clear, no aquatic plants and algae are seen at the bottom, and there are white salt crystals on the shore, the water temperature is 20°C, and the pH value is 10.5; the solid bacterial agent prepared in Example 1 is sprayed per mu 2kg, combined with some organic fertilizer (dried chicken manure or fermented cow manure) can be put in at an amount of 150kg per mu, and the pond is raised for 1 month. The pH value of the water body reaches 7.8, algae appear in the water, and the bottom of the water body is blurred and invisible (fish pond When the water quality and bottom quality of the shrimp pond are good, the water can normally reproduce aquatic plants, algae and other microorganisms and aquatic plants that fish and shrimp can eat. cannot survive, and therefore neither fish...
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