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A strain of Burkholderia tropicalis and its application

A Burkholderia, ammonia nitrogen technology, applied in the application, bacteria, fungicides and other directions, can solve the problems of inability to use nitrogen conversion, low nitrogen conversion efficiency, environmental pollution, etc., and achieve the effect of increasing absorption efficiency

Active Publication Date: 2020-07-24
郑德成 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the means of regulating nitrogen include chemical synthesis, nitrogen conversion by nitrifying bacteria and denitrifying bacteria, acid-base absorption and nitrogen fixation by rhizobia, etc., but the above methods have limitations, such as high energy consumption of chemical synthesis; Bacteria and denitrifying bacteria have low nitrogen conversion efficiency; acid-base absorption is likely to cause environmental pollution; nitrogen fixation by rhizobia is limited by the use conditions and cannot be used for nitrogen conversion in water and air
It can be seen that there is a lack of methods capable of rapidly transforming nitrogen in various fields of the environment (atmosphere, pedosphere, hydrosphere and biosphere) in the prior art.

Method used

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  • A strain of Burkholderia tropicalis and its application
  • A strain of Burkholderia tropicalis and its application
  • A strain of Burkholderia tropicalis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation of embodiment 1 Burkholderia tropicalis bacterial agent

[0044] Strain: Tropical Burkholder, preservation number: GDMCC NO.60495;

[0045] Medium: 3.0g of beef extract, 10.0g of peptone, 5g of NaCl, 18g of agar, add water to the volume to 1L, adjust the pH to 7.2, and sterilize to obtain the bacterial solution;

[0046] 1. Preparation of liquid bacterial agent

[0047] Inoculate the tropical Burkholder in the liquid culture medium, cultivate it at 25°C for 26 hours, and obtain the bacterial liquid, that is, the concentration is 2×10 8 cfu / g liquid bacterial agent.

[0048] 2. Preparation of solid bacterial agent

[0049] Add maltodextrin (according to the mass fraction of maltodextrin and account for 50%~70% content of total mass to add) in the above-mentioned liquid bacterial preparation, conventional granulation, obtain bacterial content about 1 * 10 8 cfu / g of solid bacteria.

[0050] 3. Spray preparation

[0051] Dilute the bacterial solution ...

Embodiment 2

[0052] Application of embodiment 2 Burkholderia tropicalis bacterial agent in animal husbandry

[0053] 1. Air purification in pig farms

[0054] Application site: a pig farm, the environment of the site is odorous, and the ambient temperature is 24-32°C

[0055] Bacteria agent: the spray agent prepared in Example 1, the concentration of bacteria liquid is 2.4×10 7 cfu / mL;

[0056] Application process: according to 90cfu / cm 2 Spray the bacteria agent on the ground of the farm, and the ammonia smell and the stench of pig manure will be basically eliminated after 2 hours.

[0057] 2. Air purification for cattle breeding sites

[0058] Application site: a cattle farm, the site has a strong smell, and the ambient temperature is 20-26°C;

[0059] Bacteria agent: the spray agent prepared in Example 1, the concentration of bacteria liquid is 2.4×10 7 cfu / mL;

[0060] Application process: according to 75cfu / cm 2 Spray the bacteria agent on the ground of the farm, and the bad s...

Embodiment 3

[0080] Application of embodiment 3 Burkholderia tropicalis bacterial agent in fishery

[0081] 1. Restoration of pond bottom

[0082] In a fish pond, the bottom of the pond is salinized, the water is crystal clear, no aquatic plants and algae are seen at the bottom, and there are white salt crystals on the shore, the water temperature is 20°C, and the pH value is 10.5; the solid bacterial agent prepared in Example 1 is sprayed per mu 2kg, combined with some organic fertilizer (dried chicken manure or fermented cow manure) can be put in at an amount of 150kg per mu, and the pond is raised for 1 month. The pH value of the water body reaches 7.8, algae appear in the water, and the bottom of the water body is blurred and invisible (fish pond When the water quality and bottom quality of the shrimp pond are good, the water can normally reproduce aquatic plants, algae and other microorganisms and aquatic plants that fish and shrimp can eat. cannot survive, and therefore neither fish...

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Abstract

The invention provides a strain of burkholderia tropica zheng probiotics A of which the preservation number is GDMCC NO.60495, and belongs to the technical fields of new strains of burkholderia and application thereof. The strain of burkholderia tropica zheng probiotics A has the advantages that burkholderia tropica can convert ammonia-nitrogen substances into organic nitrates, while the toxicityof ammonia gas to thalli is reduced, the energy in the nitrate conversion process can also be utilized, and ammonia nitrogen pollution in water, air and soil can also be converted, thereby achieving the effects of purifying the air and water and controlling soil hardening and salinization; the generated organic nitrates can be quickly absorbed and utilized by animals or plants after being dissolved in water, thereby being capable of improving the efficiency of absorption of livestock to the nitrogen element in food, and achieving the purpose of increasing the yield; the burkholderia tropica has the effects of degrading heavy metals, inhibiting bacteria and preventing and controlling plant diseases and pests.

Description

technical field [0001] The invention relates to the technical field of new strains of Burkholderia genus and application thereof, in particular to a strain of Burkholderia tropicalis and application thereof. Background technique [0002] Nitrogen is an essential building block of proteins, genetic material, as well as chlorophyll and other key organic molecules, and is required by all living organisms for life. Plants generally absorb inorganic nitrogen compounds such as ammonia salt or nitrate, and the nitrate is used for the synthesis of amino acids and proteins after being reduced to ammonia salt or to a stage related to ammonia salt. In contrast, animals can only use organic nitrogen compounds such as amino acids or proteins as nitrogen sources, otherwise they cannot use them. Animals use amino acids absorbed in the body as materials to synthesize their own proteins. [0003] Nitrogen is also inseparable from human life and production. Agricultural production (agricult...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P13/00C02F3/34B09C1/10A01N63/20A01P3/00A23K10/18C12R1/01
Inventor 郑德成郑娜
Owner 郑德成
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