Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of Burkholderia and its application

A technology of Burkholderia and Kholderia, applied in the field of microorganisms, to achieve the effect of increasing growth height, promoting absorption and increasing growth rate

Active Publication Date: 2022-04-12
ANHUI QIMEN COUNTY QIHONG TEA
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Tea polyphenols, also known as "tea tannins" or "tea tannins", are an important biologically active component in tea. Tea polyphenols in black tea are important precursors of theaflavins and the main indicator for improving the quality of black tea. There are no reports on the increase of total tea polyphenols by Burkholder strains

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of Burkholderia and its application
  • A kind of Burkholderia and its application
  • A kind of Burkholderia and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A Burkholderia XYQH-1, the strain has been preserved in the China Center for Type Culture Collection on January 18, 2021, and the preservation number of the Burkholderia XYQH-1 is: CCTCC M 2021105 XYQH-1.

[0031] Among them, other reagents used in the examples of the present invention were all analytically pure.

[0032] The Burkholderia XYQH-1 preserved in the present invention is isolated from the rhizosphere soil of the tea garden in Ruokeng Town, Qimen County, Huangshan City, Anhui Province. Specific steps are as follows:

[0033] 1) Screening of bacterial strains: Weigh 10.0 g of fresh soil samples, place them in a 250 mL Erlenmeyer flask filled with 90 mL of sterile water, and place them at 28-30°C, 180 r min -1Shake in a full-temperature shaking incubator for 30 min, mix well to make a soil suspension, take 100 μL of the soil suspension and add it to a 1.5 mL sterilized centrifuge tube filled with 900 μL of sterile water, and use a ten-fold gradient dilution m...

Embodiment 2

[0052] A growth-promoting application of Burkholderia XYQH-1 in tea tree cultivation, specifically as follows:

[0053] On June 1, 2020, bacteria were applied to the current year's tea seedlings (China Tea 108). The plant height of the tea seedlings was measured on August 1, 2020, two months after the tea seedlings were potted with the strain culture solution.

[0054] 1) Activation: Take Burkholderia XYQH-1 preserved in glycerin, shake the glycerol tube up and down to distribute the bacteria solution and glycerin evenly, then use a burnt and cooled inoculation loop to dip the bacteria solution in the glycerin tube, and put The inoculation loop dipped in the bacterial solution was streaked on the LB solid medium for activation, and then the LB solid plate of the activated strain was sealed with parafilm and the strain name and date were marked with a marker pen. Wherein, the formula of the LB solid medium is peptone 10g, yeast powder 5g, sodium chloride 5g, water 1000mL, agar ...

Embodiment 3

[0060] The isolated Burkholderia XYQH-1 of the present invention can also increase the total amount of tea polyphenols in tea tree leaves, and the potted treatment of tea seedlings specifically includes the following steps:

[0061] 1) Activation: Take Burkholderia XYQH-1 preserved in glycerin, shake the glycerol tube up and down to make the bacteria liquid and glycerin evenly distributed, then use a burnt and cooled inoculation loop to dip the bacteria liquid in the glycerin tube, and put The inoculation loop dipped in the bacterial solution was streaked on the LB solid medium for activation, and then the LB solid plate of the activated strain was sealed with parafilm and the strain name and date were marked with a marker pen. The formula of LB solid medium is: peptone 10g, yeast powder 5g, sodium chloride 5g, water 1000mL, agar powder 20g, pH 7.0-7.2.

[0062] 2) Cultivation: Place the marked activated strain sealed plate upside down and place it in a constant temperature in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a Burkholderia bacterium and its application, belonging to the technical field of microbial strains. The Burkholderia bacterium XYQH-1 is preserved in the China Center for Typical Culture Collection, and the preservation number is: CCTCC M 2021105; The culture solution of the strain is inoculated in the root rhizosphere soil of potted tea seedlings, which can promote the growth of tea seedlings and improve the efficacy of the total amount of tea polyphenols. The fermentation conditions of Burkholderia XYQH-1 of the present invention are easy to operate. At the same time, the Burkholderia XYQH-1 can also prepare natural microbial fertilizers, which can promote the growth of tea trees and increase the content of tea polyphenols, providing an alternative for the tea market and a new type of environmentally friendly fertilizer. Fertilizers can greatly reduce the environmental pollution caused by chemical fertilizers.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Burkholderia bacterium and an application thereof. Background technique [0002] Burkholderia belongs to the genus Burkholderia. It is a bacterium that widely exists in water, soil, plants and human body. The isolated colony is cultured on the potassium solution medium at 28°C for 3-4 days, with a diameter of 8-13 mm. After being activated or purified in LB medium for 24 hours, the diameter of the colony is about 8-10 mm, and the diameter of the colony after 3 days of cultivation Up to 15-20 mm. [0003] Tea polyphenols, also known as "tea tannins" or "tea tannins", are an important biologically active component in tea. Tea polyphenols in black tea are important precursors of theaflavins and the main indicator for improving the quality of black tea. There is no report on the increase of total polyphenols in tea leaves by Burkholder strains. The patent scr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C12R1/01
CPCC12N1/20A01N63/20
Inventor 张显晨王宁宁姜红吴锡端
Owner ANHUI QIMEN COUNTY QIHONG TEA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com