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46 results about "Inoculation loop" patented technology

An inoculation loop, also called a smear loop, inoculation wand or microstreaker, is a simple tool used mainly by microbiologists to retrieve an inoculum from a culture of microorganisms. The loop is used in the cultivation of microbes on plates by transferring inoculum for streaking. It can also be used to transfer microscopic organisms.

Preparation method of boiling antigen for vibrio parahaemolyticus serum agglutination

The invention discloses a preparation method of a boiling antigen for vibrio parahaemolyticus serum agglutination. The preparation method comprises the following steps: selecting common serotype vibrio parahaemolyticus in monitoring for resuscitation; scraping a ring of placenta by using 10 mu L of an inoculating loop, dissolving the placenta in 1 mL of normal saline to prepare a bacterial suspension, and preparing 3 tubes of bacterial suspensions from each strain; boiling the bacterial suspension in boiling water for 1 hour, 45 minutes and 30 minutes respectively; and after boiling, centrifuging at 10000 rpm for 10 minutes, discarding the supernate, and reserving the precipitate as a boiling antigen for serum agglutination. The result shows that the agglutination accuracy after boiling for 1 hour is 100%, and the agglutination degree is 2 + or above; and no significant difference exists between 45 minutes of boiling and 1 hour of boiling, and no significant difference exists between 30 minutes of boiling. Compared with a high-pressure method, the method has the advantages that the operation time is shortened by 2-3 hours, special equipment certificates are not needed, and the method is suitable for daily detection and food poisoning traceability.
Owner:BEIJING FENGTAI DISTRICT CENT FOR DISEASE CONTROL & PREVENTION

Preparation method of acinetobacter picoteri solid microbial inoculum

The invention discloses a preparation method of an acinetobacter picoteri solid fungicide, and relates to the field of a production process of a microbial solid fungicide, in particular to the preparation method of the acinetobacter picoteri solid fungicide. The invention aims to solve the problem of low viable count in the existing acinetobacter picoteri microbial inoculum. The method comprises the following steps of: 1, transferring a cryopreserved acinetobacter peteri strain into a slant activation culture medium, and culturing at a constant temperature; 2, after activation is completed, two rings of strains are scraped from the slant activation culture medium through an inoculating loop and inoculated to a seed culture medium, and a seed solution is obtained through constant-temperature shaking culture; 3, inoculating the seed solution into a conical flask filled with a fermentation culture medium for culturing; and 4, adding the acinetobacter picoteri liquid subjected to shake flask fermentation into spray drying equipment for spray drying, and adding a spray drying composite protective agent to prepare the acinetobacter picoteri powder. According to the method disclosed by the invention, the viable count and the thallus survival rate of the acinetobacter pitertii spray drying are remarkably improved. The method is used for preparing the acinetobacter picoteri solid microbial inoculum.
Owner:JILIN AGRICULTURAL UNIV

A double gauge inoculation loop push-scrapping groove mold

ActiveCN117067523Breduce encroachmentreduce connectionsButt jointPush out
The application discloses a double-specification inoculation ring push-scraping groove mold, which comprises a limiting plate one arranged in a rectangular plate body structure, a butt joint column one end symmetrically connected to the bottom end of the limiting plate one, and the other end of the butt joint column connected to the surface of a limiting plate two, the bottom end of the limiting plate one hingedly connected to a front fixed plate, and a positioning ring symmetrically arranged on the two sides of the front fixed plate. The double-specification inoculation ring push-scraping groove mold is provided with a push-scraping groove one and a push-scraping groove two arranged on the surface of a male mold plate in an interlaced and equidistant manner. Due to the symmetrical circular groove structure of the two ends of the push-scraping groove one and the push-scraping groove two, the two ends of the push-scraping groove one and the push-scraping groove two are penetrated by a pulling needle and a ejector pin rod according to production needs, the inoculation ring is inoculated in the interior of the push-scraping groove one and the push-scraping groove two through the penetration of the pulling needle and the ejector pin rod, the formed inoculation rings of different sizes are pushed out, the corresponding ejection environment is provided for the inoculation rings of different sizes, and the application range of the pulling needle and the ejector pin rod is increased.
Owner:YONGYUE MEDICAL TECH (KUNSHAN) CO LTD

Specimen bottle for collecting alveolar lavage fluid

The utility model relates to the technical field of medical instruments, and discloses a specimen bottle for collecting alveolar lavage fluid, which is characterized by comprising a bottle body (1), a first cover (2) and a second cover (6), the first cover (2) and the second cover (6) are arranged at two ends of the bottle body (1), and the first cover (2) and the second cover (6) are detachably connected with the bottle body (1) through threads. An inoculation funnel (3) is arranged at one end, connected with the second cover (6), of the bottle body (1), an inoculation sampling opening (4) is formed in the end point of the inoculation funnel (3), when the covers are tightly covered, the inoculation sampling opening (4) can be extruded to generate a sealing effect, after the first cover (2) is opened, a material needing to be inoculated is put in, and the second cover (6) can be opened during inoculation, so that the second cover (6) can be conveniently opened. And sampling by using the inoculation funnel (3). The use of a traditional inoculating loop is avoided, and the probability of pollution and / or propagation in the operation process is reduced.
Owner:BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV +1

Method for transporting and storing gonococcus

PendingCN120796144ABacteriaMicroorganism based processesBiotechnologyUltra Low Temperature Freezer
The invention provides a gonococcus transport and preservation method, which comprises the following steps: step 1, taking out one strain which is not freeze-dried from an ultralow-temperature refrigerator, placing the strain at room temperature for later use, after the strain is slightly dissolved, taking out a certain amount of bacterial liquid from a biosafety cabinet by using a pipettor, inoculating the bacterial liquid onto a pre-heated culture medium, marking lines in four regions, and then placing the culture medium in an incubator with a candle jar or a CO2 incubator at 36 DEG C for culturing for 36 hours; 2, picking pure bacteria in a biosafety cabinet by using a disposable inoculating loop, inoculating the pure bacteria to a pre-heated culture medium, and placing the culture medium in a 36-DEG C incubator with a candle jar or a CO2 incubator for culturing for 24 hours; and step 3, taking one sterile glass test tube, adding 1mL of sterilized normal saline, scraping bacterial lawn by using a disposable inoculating loop, grinding along the tube wall, putting on a vortex device, sufficiently and uniformly mixing, and adding into sterilized skim milk. The strain is subjected to freeze-drying preservation through a vacuum freeze-drying technology, so that energy expenditure for preservation of gonococcus is saved, and benefits are brought to distribution of national gonococcus interventricular quality assessment substances and transportation of national gonococcus drug-resistant monitoring point specimens.
Owner:HOSPITAL OF DERMATOLOGY CHINESE ACADEMY OF MEDICAL SCIENCES

A metal seeded ring that can be tested for impedance under power

The application relates to the field of microbial treatment, and particularly relates to a metal inoculation ring capable of testing impedance by electrification, which comprises a metal working end used for contacting and absorbing a sample, a mounting part connected with the metal working end, and a control module electrically connected with the metal working end, wherein the control module comprises a mode switching unit used for switching between a heating mode and a detection mode, a power driving module used for applying an electric current to the metal working end in the heating mode so that the metal working end generates heat to achieve sterilization, and an impedance detection module used for applying a detection electric signal to the metal working end in the detection mode to detect the absorption condition of the sample; and the control module is configured to judge the state of the sample absorbed by the metal working end according to the change of the electric characteristic parameter relative to a reference value in the detection mode. The metal ring has the functions of heating sterilization and impedance detection, and can objectively judge whether the absorption amount of the inoculation ring reaches a preset standard.
Owner:SUZHOU WANGUIYUAN PRECISION TECH CO LTD

Simple field fungal quantification pinning

The application discloses a simple field fungus quantitative inoculation needle and relates to the technical field of inoculum inoculation tools. The upper end of a connector is connected with the working end of a liquid quantitative control flowmeter, the lower end opening of the connector is communicated with the upper end of a connecting pipe, the outer circumferential surface of the connecting pipe is slidably connected with a sleeve, the lower end of the sleeve is communicated with a puncture body, and the upper end of the sleeve movably installs a fastening nut. The simple field fungus quantitative inoculation needle can keep the penetration depth of multiple puncture bodies consistent when the multiple puncture bodies inoculate fungi on fruits through the double distance control of the sleeve and the limiting rod, and the operation is convenient. Meanwhile, the telescopic protection assembly can meet the inoculation environment of the field, can effectively block external miscellaneous bacteria, and has strong environmental adaptability.
Owner:SHANDONG INST OF POMOLOGY

Baking and sterilization machine

1. The name of this design product: All-in-one baking and sterilization machine. 2. Purpose of this design product: used for drying specimens and sterilizing inoculation loops. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1.
Owner:ZHUHAI LIKANG TECHNOLOGY CO LTD

Automatic inoculation equipment for fungi

The utility model relates to the technical field of fungus planting, in particular to fungus automatic inoculation equipment which comprises a rack, a conveying belt, a pair of positioning mechanisms, a lifting inoculation mechanism and a negative pressure suction mechanism. The conveying belt can be rotatably mounted on the rack and is used for conveying the strain frames with the mushroom sticks; the positioning mechanisms are arranged on the portions, located on the two sides of the conveying belt, of the rack correspondingly, and the positioning mechanisms can be matched with each other to clamp the strain frames conveyed on the conveying belt; the lifting inoculation mechanism is mounted above the area, between the two positioning mechanisms, of the rack, and the lifting inoculation mechanism can be matched with the two positioning mechanisms to inoculate mushroom sticks on the strain frame; the negative pressure suction mechanism is arranged on the rack, and the suction end of the negative pressure suction mechanism is arranged on the periphery of the lifting end of the lifting inoculation mechanism. The automatic fungus inoculation equipment can effectively remove matrix chippings brought out in the inoculation process, and the neatness of the inoculation environment is guaranteed.
Owner:GUANGXI UNIV +1

Rapid mass spectrum identification method for filamentous fungi cultured in enrichment culture bottle

The invention provides a rapid mass spectrum identification method for filamentous fungi cultured in an enrichment culture bottle, and relates to the field of microbiological detection. The method comprises the following steps: S1, selecting a culture bottle subjected to enrichment culture; s2, using sterile scissors or other sharp instruments to open a rubber plug of the enrichment culture bottle; s3, lightly extending a disposable sterile inoculating loop into the culture bottle, accurately picking a ring of small fungal clusters, and placing the small fungal clusters on a disposable plate cover; s4, 10 microliters of formic acid is added to a disposable plate cover, and grinding is conducted on the plate cover for 50-100 times through disinfected surgical forceps; and S5, finally coating a mass spectrum target plate with the grinding liquid, and carrying out mass spectrum detection. According to the method, the floccules of the fungal clusters are directly picked from the enrichment culture bottle, so that seed transformation failure caused by the fact that the floccules in the enrichment culture bottle are not easy to extract by an injector is avoided, the long growth process after seed transformation of the filamentous fungi does not need to be waited, the picked fungal clusters are directly treated and identified, and the whole detection period is greatly shortened.
Owner:TAIYUAN CENT HOSPITAL

Plate scribing equipment and bioassay system

The utility model relates to the technical field of bioassay, and discloses plate scribing equipment and a bioassay system.The plate scribing equipment is characterized by comprising an inoculating loop, a first strain storage area, a plate supporting device, a movable scribing device and a first lifting device; and the inoculating loop is suitable for inoculating strains. The first strain storage area is suitable for placing vessels in which strains are stored; the plate supporting device is suitable for placing a plate. The inoculating loop is connected with the movable lineation device, and the movable lineation device is suitable for driving the inoculating loop to move horizontally so as to drive the inoculating loop to move between the position above the plate supporting device and the position above the first strain storage area and complete the lineation action. The device can replace most manual operation to carry out scribing operation, and does not need to worry that the working efficiency is reduced when the number of measured items is large and tasks are heavy. Compared with complete manual marking operation, the marking operation is faster, more timely and more accurate.
Owner:蒙牛乳业(宁夏)有限公司 +1

Batch sterilization device containing integrated inoculating loop

The invention discloses a batch sterilization device containing an integrated inoculating loop, the batch sterilization device comprises a cover, an upper part, a lower part and the integrated inoculating loop, the integrated inoculating loop is made of a metal wire through the steps of bending, winding and the like, and the integrated inoculating loop comprises an elastic head, a handheld rod and a clamping and fixing position. With the adoption of the technical scheme, related personnel can utilize common auxiliary devices such as a clamp holder, a hook and a rotator to quickly and conveniently manufacture the integrated inoculating loops in batches at low cost. A step and a partition plate structure are arranged in the sterilization device, the inner inoculating loop is effectively divided into different areas, and the heights of the inoculating loops in the areas are different; the height of the device can be flexibly and telescopically adjusted to adapt to inoculating loops with different heights. The device can be used for batch sterilization of the integrated inoculating loop, and due to the design, the sterilized inoculating loop can be used in batches, so that the working efficiency is improved, the cross contamination is effectively reduced, and the accuracy and the reliability of subsequent experimental results are ensured.
Owner:FUJIAN PROVINCIAL PROD QUALITY INSPECTION INST (FUJIAN PROVINCIAL DEFECTIVE PROD RECALL TECH CENT)

Sterile inoculation operation device for plant cell group solid culture

The invention discloses a sterile inoculation operation device for plant cell group solid culture, and belongs to the technical field of plant culture, the sterile inoculation operation device comprises a shell module and knife shell modules, the shell module is internally provided with a locking cavity and a storage cavity which are opposite to each other, and the two knife shell modules are alternately assembled in the locking cavity; according to the invention, the circulating switching of the two groups of knife shell modules between an operation state and a disinfection state is realized through an alternate working mechanism, one group executes a cutting task, and the other group synchronously carries out disinfection preparation, so that the operation interval time is greatly shortened, the process interruption caused by pollution or replacement of the knife is avoided, and the working efficiency is improved. Through modular design and automatic rotation, the system remarkably improves the operation efficiency, cleanliness reliability and flow continuity of a sterile inoculation link in plant cell group solid culture, and is suitable for plant biotechnology experiments and production environments with high repeatability and high cleanliness requirements.
Owner:HUAXIA HUABEN AGRICULTURAL TECHNOLOGY (SHANGHAI) CO LTD

Combined inoculating head with detachable alloy inoculating loop

ActiveCN224199377UPreserve processabilityRetain material advantagesBioreactor/fermenter combinationsBiological substance pretreatmentsMicroorganismAlloy
The utility model belongs to the technical field of microbial culture inoculation equipment, and particularly relates to a combined inoculation head with a detachable alloy inoculation loop. Comprising a connecting cylinder, a mounting seat, an alloy inoculating loop, a magnetic suction head and a reset spring, the connecting cylinder is vertically arranged, the mounting seat is arranged in the connecting cylinder in a manner of sliding up and down, and the alloy inoculating loop is of a C-shaped structure; and the magnetic suction head is embedded in the top opening of the connecting cylinder. In order to meet the requirements of batch production and rapid assembly of automatic marking heads, the combined inoculating head with the detachable alloy inoculating loop provides a structure of the detachable alloy inoculating loop, so that the body processing and material advantages of the alloy inoculating loop are reserved; and meanwhile, an effective mode is provided for connection and assembly of an automatic scribing structure, the alloy inoculating loop serves as a detachable independent structure and can be independently machined, disinfected and maintained, finally, the alloy inoculating loop is assembled and used in a unified mode, and the use and maintenance cost of batch scribing equipment can be effectively reduced.
Owner:WUHAN XUDEXI BIOTECHNOLOGY CO LTD

Sputum collector

The utility model relates to the technical field of sputum collectors, in particular to a sputum collector which comprises a sterile plastic barrel with the upper end and the lower end open, a bottom cover is connected to the lower end of the outer wall of the sterile plastic barrel in a threaded mode, a water absorption sponge located in the sterile plastic barrel is arranged on the inner side wall of the bottom cover, and a filter screen is fixedly connected to the interior of the sterile plastic barrel. The upper end of the outer wall of the sterile plastic barrel is in threaded connection with a top cover, the upper end of the top cover is fixedly connected with two symmetrically-distributed clamping blocks, a brush rod and an inoculating ring are clamped into the two clamping blocks respectively, an annular groove is formed in the upper end of the top cover, and a dustproof cover is connected into the annular groove in an interference fit mode. The size of the water absorption sponge is matched with the size of the sterile plastic barrel, so that saliva and sputum can be separated, the saliva cannot flow back after separation, the hair brush and the inoculating ring are combined, the separated sputum can be preliminarily rubbed through the hair brush, the sputum is cut off to a certain degree, and the sputum can be conveniently picked through the inoculating ring.
Owner:WUXI REGULAR PRECISION MEDICAL TESTING CO LTD

Inoculation tool for 50L bacitracin zinc fermentation tank

The utility model discloses a 50L bacitracin zinc fermentation tank inoculation tool, and belongs to the technical field of biological strain inoculation. Comprising an inoculating loop, an adjusting base and a windproof assembly, the adjusting base is rotationally installed at the bottom of the inoculating loop, an alcohol cotton groove with an opening in the top is formed between the adjusting base and the inoculating loop, the windproof assembly is vertically and slidably arranged in the alcohol cotton groove in a covering mode, abutting rods are symmetrically installed at the top of the adjusting base, and the abutting rods are matched with the windproof assembly in an abutting mode. The inner wall of the inoculating loop is fixedly connected with an arc-shaped track for the abutting rod to move, and an outlet is formed in one end of the arc-shaped track. By means of alcohol cotton burning in the alcohol cotton groove, the pollution risk in the inoculation process is effectively reduced, the sterile environment in the fermentation process is guaranteed, the inoculation operation is more flexible due to the design of the adjusting base and the arc-shaped track, the windproof assembly can be rapidly lifted by screwing the adjusting base, and the stability of flame burning is enhanced.
Owner:JIANGSU JIUYANG BIOLOGICAL PHARMA

Accurate quantitative inoculating loop for urine culture

The utility model discloses an accurate quantitative inoculating loop for urine culture, which relates to the field of medical detection auxiliary tools and comprises a cylindrical grab handle, and a first stepped hole groove and a second stepped hole groove which are small in bottom and big in top are formed in the bottom of the grab handle along the height direction; the first stepped hole groove is composed of a first lower stepped hole and a first upper stepped hole, and the second stepped hole groove is composed of a second lower stepped hole and a second upper stepped hole. A marking piece and an inoculating ring piece which can move up and down are respectively arranged in the first stepped hole groove and the second stepped hole groove; a first through groove and a second through groove which are communicated with the first upper step hole and the second upper step hole are respectively formed in the outer wall of the grab handle; a first push plate and a second push plate are arranged on the outer wall of the grab handle, and one end of the first push plate and one end of the second push plate penetrate through the first through groove and the second through groove and are arranged at the upper ends of the scribing piece and the inoculating ring piece respectively; the inoculating loop is not easy to pollute, and the operation is simple; meanwhile, the authenticity and the accuracy of quantification of the inoculating loop are realized.
Owner:WUXI REGULAR PRECISION MEDICAL TESTING CO LTD

An inoculation port and a simple fermentation tank

This utility model relates to an inoculation port, and more particularly to an inoculation port and a simple fermentation tank. It belongs to the technical field of edible mushroom fermentation equipment. An inoculation port, fixed to the bottleneck of a plastic bucket during use, includes an annular alcohol tank and a hollow cylindrical inner body with an outer diameter adapted to the inner diameter of the bottleneck. The annular alcohol tank is fitted around the outer periphery of the hollow cylindrical inner body, or integrally connected to the outer periphery of the hollow cylindrical inner body. This utility model's inoculation port ensures efficient sterilization of the inoculation ring while fully considering the usage scenarios of non-professional users such as farmers. It combines ease of operation and cost control, providing a practical technical solution for the promotion and application of small-scale edible mushroom cultivation technology.
Owner:DEQING WUKANG SMALL LEAF EDIBLE MUSHROOM FARM

Screening method of ganoderma lucidum strain suitable for fermenting brown rice

The invention discloses a ganoderma lucidum strain screening method suitable for fermenting brown rice, which comprises the following steps: step 1, preparing an experimental material ganoderma lucidum strain, activating the ganoderma lucidum strain, taking 1-2 rings of the ganoderma lucidum strain stored at 4 DEG C by using an inoculating ring in an ultra-clean workbench, inoculating the ganoderma lucidum strain into a sterilized PDB liquid culture medium, uniformly shaking, taking out the ganoderma lucidum strain, and taking out the ganoderma lucidum strain; activating in a shaking table at 28 DEG C and 180 r / min, continuously activating for 72 hours, completing the activation when the culture medium becomes turbid and a large amount of hyphae appear, and storing at 4 DEG C for later use; step 2, preparing a ganoderma lucidum brown rice fermentation product, screening brown rice raw materials and removing impurities, and according to the ganoderma lucidum strain screening method suitable for fermenting brown rice, through comprehensive evaluation of changes of ganoderma lucidum triterpenes content in the brown rice fermentation process of four ganoderma lucidum strains, it is found that the ganoderma lucidum ACCC 51677 strain is more suitable for brown rice fermentation; the fermentation period is short, the content peak value is high, the stability is good, and a scientific basis is provided for efficient utilization of ganoderma lucidum resources.
Owner:GUIZHOU INST OF BIOTECHNOLOGY (GUIZHOU KEY LAB OF BIOTECHNOLOGY GUIZHOU POTATO RES INST GUIZHOU FOOD PROCESSING RES INST) +1

Inoculating loop scribing device

The invention relates to the technical field of inoculating devices, in particular to an inoculating loop scribing device. Comprising a manipulator, a pipetting module, first pipetting gun heads, a culture dish and a storage rack, the clamping end of the manipulator clamps the pipetting module, the lower end of the pipetting module is connected with the first pipetting gun heads in a clamped mode, and the storage rack is used for storing the multiple first pipetting gun heads; the first pipette tip comprises a fixing part, a connecting part, filter cotton, a core rod and an inoculating loop, the fixing part is clamped with the pipette module, the filter cotton is arranged in the connecting part, one end of the core rod extends into the connecting part and is clamped with the connecting part, and the other end of the core rod is integrally connected with the inoculating loop; a first camera is arranged on the manipulator, the first camera is used for shooting a first image in real time, the first camera is electrically connected with a controller, and the controller processes the obtained first image and plans a movement route from the manipulator to a lineation starting position of a culture dish when the manipulator clamps a pipetting module provided with a first pipetting gun head; and the inoculation scribing efficiency is improved.
Owner:ZHUHAI MEIHUA MEDICAL TECH LTD

Strong grabbing sample inoculating loop

The utility model discloses a strong grabbing sample inoculating loop, which belongs to the technical field of sampling equipment and comprises a rod body, a plurality of storage grooves are arranged on the outer wall of an inoculating loop body, and a plurality of dipping columns distributed in an array are fixedly connected to the outer wall of the inoculating loop body. A plurality of dipping mechanisms which are distributed in an array mode and located between every two adjacent dipping columns are arranged on the outer wall of the inoculating loop body, a plurality of storage grooves are formed in the outer wall of the inoculating loop body, the surface area of the inoculating loop body is increased, and the dipping columns which are distributed in an array mode are fixedly connected to the outer wall of the inoculating loop body; meanwhile, the contact surface with a sample is increased through a threaded groove and an arc-shaped block, more samples can be dipped, a first dipping plate and two second dipping plates are arranged, so that the dipped samples are not prone to losing from the inoculating loop body, and the inoculating loop has the advantages that the needed samples can be conveniently dipped, the sampling effect is better, the inoculating loop is convenient to disassemble, assemble and replace, and the working efficiency is improved. And the sampling efficiency is higher.
Owner:WUXI REGULAR PRECISION MEDICAL TESTING CO LTD

Inoculation loop (electrocautery)

ActiveCN309820906SMicroorganismAnimal science
1. The name of the design product: inoculation loop (electrical cauterization type). 2. The use of the design product: when cultivating microorganisms, take out the microorganisms and put them into the culture medium. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view. 5. The top view is omitted because it is clearly expressed in other views.
Owner:贾会生

Metal inoculating loop capable of being electrified to test impedance

The invention relates to the field of microbiological treatment, in particular to a metal inoculating loop capable of being electrified to test impedance, which comprises a metal working end used for contacting and staining a sample; the mounting part is connected with the metal working end; the control module is electrically connected with the metal working end; wherein the control module comprises a mode switching unit used for switching between a heating mode and a detection mode; the power driving module is used for applying current to the metal working end in the heating mode, so that the metal working end is heated to realize sterilization; the impedance detection module is used for applying a detection electric signal to the metal working end in the detection mode so as to detect the sample staining condition; and the control module is configured to judge the state of a sample stained by the metal working end according to the change of the electrical characteristic parameter relative to a reference value in a detection mode. The metal ring has the functions of heating sterilization and impedance detection at the same time, and whether the dipping amount of the inoculating loop reaches a preset standard or not is objectively judged.
Owner:SUZHOU WANGUIYUAN PRECISION TECH CO LTD

Cryoprobe cooling device

This utility model provides an inoculation loop cooling device, relating to the field of inoculation loop cooling technology. It includes a support frame, a temperature detection component, a cooling component, clamps, and an indicator module. Several sets of clamps are arranged in a horizontal array, allowing the inoculation loop to be held in place. The cooling component includes a fan and a distribution air rod. The distribution air rod has air holes connected to the fan, with the air outlet direction along the length of the inoculation loop. This design, by providing air holes that blow air along the length of the inoculation loop, can cover most of the end of the inoculation loop, improving cooling efficiency. Furthermore, a temperature detection component is included to detect the temperature of the inoculation loop. When the temperature detection component detects that the temperature of the inoculation loop is lower than a set value, the indicator module switches to an indicating state, allowing the operator to easily determine whether the inoculation loop has been cooled completely, thus contributing to improved inoculation efficiency.
Owner:HANGZHOU D A GENETIC ENG

Strain culture device for white spirit brewing

The invention relates to the technical field of white spirit processing, and particularly discloses a strain culture device for white spirit brewing, which comprises a separation inoculation mechanism, a strain mechanism, a culture dish mechanism and a controller, the strain mechanism is arranged on the left side of the separating and inoculating mechanism; the culture dish mechanism is arranged on the right side of the separating and inoculating mechanism; and the controller is arranged outside the separating and inoculating mechanism. Manual contact is avoided through whole-process automatic operation, the risk that infectious microbes are introduced into the environment is greatly reduced by combining a closed operation space and sterile airflow protection, flame forms are restrained, it is ensured that all parts of the inoculating loop can reach the high temperature sufficient for sterilization, the risk that a culture medium is polluted due to incomplete sterilization is effectively reduced, and the safety of the inoculating loop is improved. Meanwhile, rapid cooling can be achieved in the cooling link, secondary pollution in the cooling process is avoided, and the sterile effect of strain purification is remarkably improved.
Owner:INNER MONGOLIA MONGOLIANKING IND CO LTD

Stoma urine culture device

The invention discloses a stoma urine culture device, and belongs to the technical field of culture equipment.The stoma urine culture device comprises a first circular shell, a second circular shell is fixedly arranged on the first circular shell, and a partitioning assembly and a calling assembly are arranged in the first circular shell. The problem of time superposition of single sample culture is avoided, a fan-shaped sliding plate I and a fan-shaped sliding plate II are in gapless fit with a storage chamber, and when a position adjusting sliding frame is jointed with a fan-shaped strip plate, the end part of an air guide pipe can be sealed, external air interference is isolated, a stable and pollution-free culture condition is provided for the growth of microorganisms in urine; the culture dish is simple in structure and convenient to operate, relative stability of the culture dish and the culture dish can be maintained under the action of a tension spring, sample leakage or non-uniform strain distribution in the culture process is effectively avoided, the opening and closing degree and opportunity of the dish cover can be accurately controlled by arranging a calling assembly, sufficient operation space is provided for the inoculation loop to inoculate a urine sample, and meanwhile infectious microbe pollution in the inoculation process is avoided.
Owner:SHANDONG PROVINCIAL HOSPITAL AFFILIATED TO SHANDONG FIRST MEDICAL UNIVERSITY (SHANDONG PROVINCIAL HOSPITAL)

Dual-mode portable electrode type inoculating loop sterilizer

The utility model discloses a dual-mode portable electrode type inoculating loop sterilizer, and belongs to the technical field of microorganism experiment instruments. The electrode comprises electrode sleeves, an insulating sleeve and a metal inoculation wire, the two electrode sleeves are connected through the insulating sleeve, and the two electrode sleeves and the insulating sleeve are sleeved on the metal inoculation wire; the longitudinal section of the M-shaped inoculation box is in an M-shaped curved surface form, first electrodes are arranged at two top ends of the M-shaped inoculation box, and a second electrode is arranged in a groove of the M-shaped inoculation box; the width of the groove is matched with the diameter of the inoculating loop; the first electrode is connected with a pressure sensor, the second electrode is connected with a photosensitive sensor, and the pressure sensor and the photosensitive sensor are connected with a control circuit. The power supply is connected with the control circuit and has a charging function, and 220V alternating current is converted into 5V direct current. Under different indoor and outdoor power supply conditions, the device disclosed by the utility model is adaptive to specific operation procedures of microbial passage, separation and purification experiments, and convenient and efficient sterilization and biomass reduction are realized.
Owner:PKU HKUST SHENZHEN HONGKONG INSTITUTION

A flavoring viscosity-reducing agent and its application in reconstituted tobacco concentrate

The application discloses a flavoring and viscosity-reducing microbial agent and application thereof in reconstituted tobacco concentrate. The flavoring and viscosity-reducing microbial agent is prepared by the following method: (1) culture and enrichment of microbial bodies: Klebsiella sp. HNYJ-1 monoclone preserved in the China General Microbiological Culture Collection Center with a preservation number of CGMCC No. 24327 is inoculated into LB liquid culture medium by using an inoculating loop, and is cultured to obtain original seeds; and (2) the flavoring and viscosity-reducing microbial agent is obtained by gradient induction of the microbial bodies. The flavoring and viscosity-reducing microbial agent is used for preparing reconstituted tobacco concentrate with flavoring and viscosity reduction. After treatment of the reconstituted tobacco concentrate with the flavoring and viscosity-reducing microbial agent, the contents of protein, pectin and starch in the reconstituted tobacco concentrate are reduced, the fiber is basically unchanged, and the viscosity is reduced; meanwhile, the contents of alcohol, aldehyde ketone and ester flavoring substances in the reconstituted tobacco concentrate are obviously increased, the content of heterocyclic substances is increased, and the total amount of flavoring substances is increased.
Owner:CHINA TOBACCO HENAN IND CO LTD

A disposable inoculation loop ejection device

ActiveCN224430567UInoculation loopPush-button
This application provides a disposable inoculation loop ejection device. The device includes: a housing, an inoculation loop container, a spring-loaded assembly, and a central rod; a spring-loaded hole is formed on the top surface of the housing; the inoculation loop container is disposed inside the housing with its bottom surface inclined; the spring-loaded assembly includes a spring-loaded button, a metal rod, an inoculation loop clamp, and a spring; the spring-loaded button is located on the top surface of the housing; the metal rod is connected to the spring-loaded button and the inoculation loop clamp; the inoculation loop clamp passes through the inoculation loop container and has a gripping structure at one end, and is movably connected to the central rod at the other end; the spring is connected to the outside of the inoculation loop container and the inoculation loop clamp; the central rod is located at the bottom of the housing. When the spring-loaded button is pressed, the inoculation loop clamp moves downwards; when the spring-loaded button is released, the spring drives the inoculation loop clamp to reset, and simultaneously, the gripping structure of the inoculation loop clamps the disposable inoculation loop inside the inoculation loop container and ejects it through the spring-loaded hole. This disposable inoculation loop ejection device has a simple structure, is easy to operate, and avoids contamination.
Owner:李静虹 +1