78 results about "Inoculation loop" patented technology

The invention discloses a preparation method for kitasamycin industrial production strains, which comprises the main steps: performing plate streaking on an original strain to prepare a single colony; transferring the single colony onto an inclined plane, culturing same for a proper time; then scraping spores on the inclined plane with an inoculation loop, and preparing a freeze-dried strain tube with a vacuum freeze-drying method; preparing a spore suspension by using the freeze-dried strain tube, coating the oblique plane with the spore suspension, culturing the spore suspension for a proper time; then scraping spores on the oblique plane with the inoculation loop to prepare a spore and milk suspension; subpackaging the spore and milk suspension and performing freezing preservation so as to prepare a spore and milk freezing tube; melting the spore and milk freezing tube, and transferring same to a seed culture medium, performing shake culture by bottle shaking for a proper time, and then transferring to a seed tank. The method overcome the defects of poor synchronization of inoculation production of inclined strain production, nonuniform growth cycle of seed bottles and frequency of batch number change. The preparation method disclosed by the invention is simple and convenient to operate, the inoculum age of strains and consistency of quality of seeds are improved, the batch number change of seeds is reduced, and the preparation method is very suitable for kitasamycin industrial production.

A disposable inoculation loop assembly comprising a test tube and a tube stopper adapted to fit into a free end of the test tube. A first end of the stopper faces into the interior of the test tube substantially hermetically sealing the interior of the test tube. The assembly further includes an inoculation loop fixed to the tube stopper and extending into the interior of the test tube to a predetermined distance when the stopper is positioned in the free end of the test tube. The assembly when assembled is sterilized and pre-evacuated to a predetermined vacuum which is sufficient to draw a volume of a bodily liquid to be sampled into the test tube. The volume is of such an amount so that the liquid level in the test tube substantially just covers a predetermined portion of the inoculation loop.

The invention discloses a method for improving yield of fruiting bodies of cordyceps takaomontana. The method comprises the steps of activating an ustilago esculenta GZUIFR-DX091 bacterial strain by applying a PDA culture medium, picking three loops for the activated bacterial strain by applying an inoculating loop to access into the ustilago esculenta culture medium, centrifuging culture liquor for 5 minutes at 5000r / min after culturing for 5 days at 28 DEG C at 160r / min, and getting liquid supernatant to obtain fermentation liquor of the ustilago esculenta GZUIFR-DX091 bacterial strain; infecting silkworms with cordyceps takaomontana, and starting producing fruiting bodies for later use when the infected silkworms are hardened; pre-sterilizing a culture container, and tiling single layer or double layers of sterilized water-absorbing paper at the bottom of the container; slowly adding the prepared fermentation liquor of the ustilago esculenta GZUIFR-DX091 bacterial strain onto the water-absorbing paper at the bottom of the container, placing the infected silkworms into a container which is added with the cane shoot fermentation liquor, and carrying out culture under light for 12 hours at 20-23 DEG C, wherein the culture temperature can be lowered by 5 DEG C for culturing at a dark culture stage. In the culture process, if the added fermentation liquor is dried, the fermentation liquor is needed to be timely complemented; the fruiting bodies of cordyceps takaomontana can be harvested after culturing for 3-4 weeks. The method disclosed by the invention can greatly improve the yield of the fruiting bodies of cordyceps takaomontana.

The invention discloses a method for improving the yield of astaxanthin in a wild phaffia rhodozyma strain by using an inorganic-microorganism hybrid system. The method comprises the following steps of: shake flask seed culture: taking an inoculating loop strain from an original slant strain slant of phaffia rhodozyma, inoculating a seed culture medium with the inoculating loop strain, and performing shake flask culture at 22 + / -5 DEG C and at the rotating speed of 180-240 rpm for 24-72 hours to obtain a seed culture solution; and shake flask fermentation culture: inoculating a carbon nitride nanosheet fermentation medium with the concentration of 0.2-6 g / L with the seed culture solution according to the volume ratio of 10%, and culturing a shake flask at the temperature of 22 + / -5 DEG C and the rotating speed of 180-240 rpm for 48-108 h, wherein the fermentation culture process is performed under the condition that the whole process is light or completely dark. According to the method, the yield of the astaxanthin of the phaffia rhodozyma can be remarkably increased by a method for assembling a hybrid system by using light-driven carbon nitride nanosheets and the phaffia rhodozyma.

The invention relates to an automatic scribing equipment and a scribing method for a microorganism sample pretreatment system. The automatic scribing equipment comprises an inoculation table, an XZ double-axis linear module is installed above the inoculation table, an inoculation ring capable of controlling rotation is installed on a sliding block of the Z-axis linear module, a Y-axis linear module is installed below the inoculation table, and a Y-axis linear module is installed below the Y-axis linear module. A through groove is formed in the inoculation table and located over the Y-axis linear module, a suction cup capable of moving in the through groove is installed on a sliding block of the Y-axis linear module, and the suction cup is connected with a vacuum generator through an air pipe. The scribing equipment is diverse in scribing form, high in scribing efficiency and high in automation degree.

The invention relates to a microbe separation and identification lineation marker which effectively solves the problems that manual operation is needed in the existing microbe lineation marking process, cross infection is prone to occurring, rapid linkage of the lineation marking process cannot be achieved, and the operation efficiency cannot be improved. According to the technical scheme, the device comprises a base, an alcohol lamp and a shell, a rotating column is coaxially and rotatably connected to the upper end of the base, mounting fixing sleeves are fixedly connected to the left side and the right side of the rotating column, sliding limiting sleeves are coaxially and slidably connected into the mounting fixing sleeves, and inoculating rings are detachably connected into the sliding limiting sleeves; two hydraulic cylinders communicating with the mounting fixing sleeves are arranged in the rotating column, lifting threaded sleeves are connected into the hydraulic cylinders in an up-down sliding mode, adjusting lead screws are coaxially connected to the lifting threaded sleeves in a threaded mode, and limiting shaft rods are connected into the adjusting lead screws in an up-down sliding mode; and the two limiting shaft rods are coaxially and fixedly connected with a large belt wheel and a small belt wheel which are connected through a belt respectively, and the limiting shaft rods are externally connected with a driving source. The structure is simple and practicability is high.

The invention discloses a microbial preparation foam drainage gas production process which comprises the following steps: preparing diluents with different concentrations, inoculating thalli onto the diluents by using an inoculating loop, and then preserving at constant temperature to complete microbial culture operation; the cultivated microorganisms are injected into the stratum of the natural gas well, and meanwhile a foam scrubbing agent is injected into an oil pipe through foam scrubbing agent injection equipment; the method has the beneficial effects that microorganisms cultivated in different environments are injected into the stratum of the natural gas well at the same time, a certain amount of foam scrubbing agent is injected into the oil pipe to be matched with the oil pipe, the purpose of fast foam scrubbing gas production can be achieved, and the purpose of fast foam scrubbing gas production can be achieved; moreover, the microorganisms can generate a biosurfactant and various secondary metabolites in the gas well, a good cleaning effect is achieved on the near-wellbore zone of the well bottom, and the metabolites are protein, so that the environment is not polluted, and the pipeline flow is not corroded.

The invention discloses a lactic acid bacteria separating, screening and culturing device, and relates to the technical field of lactic acid bacteria separating and culturing. The lactic acid bacteriaseparating, screening and culturing device comprises a constant-temperature box, supporting bases are arranged at the bottom of the constant-temperature box, the multiple supporting bases are arranged, a disinfection box is fixedly connected to the top of the constant-temperature box, supporting plates are connected between the inner walls of two sides of the constant-temperature box in a slidingmode, culture dishes are arranged at the tops of the supporting plates, the number of the support plate and the culture dish are multiple, and a first clamping plate and a second clamping plate are correspondingly and fixedly connected between the inner walls, close to the top, of two sides of the disinfection box. According to the device, by arranging the disinfection box, rapid disinfection ofa inoculating loop is realized, the defect that the inoculating loop needs to be disinfected by an alcohol lamp every time the inoculating loop is used during streak separation of lactic acid bacteriaby a plate streak method is avoided, and the inoculating loop is placed in the disinfection box and can be directly taken out for use during use.