25 results about "Inoculation loop" patented technology

The invention discloses a preparation method of a boiling antigen for vibrio parahaemolyticus serum agglutination. The preparation method comprises the following steps: selecting common serotype vibrio parahaemolyticus in monitoring for resuscitation; scraping a ring of placenta by using 10 mu L of an inoculating loop, dissolving the placenta in 1 mL of normal saline to prepare a bacterial suspension, and preparing 3 tubes of bacterial suspensions from each strain; boiling the bacterial suspension in boiling water for 1 hour, 45 minutes and 30 minutes respectively; and after boiling, centrifuging at 10000 rpm for 10 minutes, discarding the supernate, and reserving the precipitate as a boiling antigen for serum agglutination. The result shows that the agglutination accuracy after boiling for 1 hour is 100%, and the agglutination degree is 2 + or above; and no significant difference exists between 45 minutes of boiling and 1 hour of boiling, and no significant difference exists between 30 minutes of boiling. Compared with a high-pressure method, the method has the advantages that the operation time is shortened by 2-3 hours, special equipment certificates are not needed, and the method is suitable for daily detection and food poisoning traceability.

The application discloses a double-specification inoculation ring push-scraping groove mold, which comprises a limiting plate one arranged in a rectangular plate body structure, a butt joint column one end symmetrically connected to the bottom end of the limiting plate one, and the other end of the butt joint column connected to the surface of a limiting plate two, the bottom end of the limiting plate one hingedly connected to a front fixed plate, and a positioning ring symmetrically arranged on the two sides of the front fixed plate. The double-specification inoculation ring push-scraping groove mold is provided with a push-scraping groove one and a push-scraping groove two arranged on the surface of a male mold plate in an interlaced and equidistant manner. Due to the symmetrical circular groove structure of the two ends of the push-scraping groove one and the push-scraping groove two, the two ends of the push-scraping groove one and the push-scraping groove two are penetrated by a pulling needle and a ejector pin rod according to production needs, the inoculation ring is inoculated in the interior of the push-scraping groove one and the push-scraping groove two through the penetration of the pulling needle and the ejector pin rod, the formed inoculation rings of different sizes are pushed out, the corresponding ejection environment is provided for the inoculation rings of different sizes, and the application range of the pulling needle and the ejector pin rod is increased.

The application relates to the field of microbial treatment, and particularly relates to a metal inoculation ring capable of testing impedance by electrification, which comprises a metal working end used for contacting and absorbing a sample, a mounting part connected with the metal working end, and a control module electrically connected with the metal working end, wherein the control module comprises a mode switching unit used for switching between a heating mode and a detection mode, a power driving module used for applying an electric current to the metal working end in the heating mode so that the metal working end generates heat to achieve sterilization, and an impedance detection module used for applying a detection electric signal to the metal working end in the detection mode to detect the absorption condition of the sample; and the control module is configured to judge the state of the sample absorbed by the metal working end according to the change of the electric characteristic parameter relative to a reference value in the detection mode. The metal ring has the functions of heating sterilization and impedance detection, and can objectively judge whether the absorption amount of the inoculation ring reaches a preset standard.

The utility model belongs to the technical field of microbial culture inoculation equipment, and particularly relates to a combined inoculation head with a detachable alloy inoculation loop. Comprising a connecting cylinder, a mounting seat, an alloy inoculating loop, a magnetic suction head and a reset spring, the connecting cylinder is vertically arranged, the mounting seat is arranged in the connecting cylinder in a manner of sliding up and down, and the alloy inoculating loop is of a C-shaped structure; and the magnetic suction head is embedded in the top opening of the connecting cylinder. In order to meet the requirements of batch production and rapid assembly of automatic marking heads, the combined inoculating head with the detachable alloy inoculating loop provides a structure of the detachable alloy inoculating loop, so that the body processing and material advantages of the alloy inoculating loop are reserved; and meanwhile, an effective mode is provided for connection and assembly of an automatic scribing structure, the alloy inoculating loop serves as a detachable independent structure and can be independently machined, disinfected and maintained, finally, the alloy inoculating loop is assembled and used in a unified mode, and the use and maintenance cost of batch scribing equipment can be effectively reduced.

The utility model relates to the technical field of sputum collectors, in particular to a sputum collector which comprises a sterile plastic barrel with the upper end and the lower end open, a bottom cover is connected to the lower end of the outer wall of the sterile plastic barrel in a threaded mode, a water absorption sponge located in the sterile plastic barrel is arranged on the inner side wall of the bottom cover, and a filter screen is fixedly connected to the interior of the sterile plastic barrel. The upper end of the outer wall of the sterile plastic barrel is in threaded connection with a top cover, the upper end of the top cover is fixedly connected with two symmetrically-distributed clamping blocks, a brush rod and an inoculating ring are clamped into the two clamping blocks respectively, an annular groove is formed in the upper end of the top cover, and a dustproof cover is connected into the annular groove in an interference fit mode. The size of the water absorption sponge is matched with the size of the sterile plastic barrel, so that saliva and sputum can be separated, the saliva cannot flow back after separation, the hair brush and the inoculating ring are combined, the separated sputum can be preliminarily rubbed through the hair brush, the sputum is cut off to a certain degree, and the sputum can be conveniently picked through the inoculating ring.

The utility model discloses a 50L bacitracin zinc fermentation tank inoculation tool, and belongs to the technical field of biological strain inoculation. Comprising an inoculating loop, an adjusting base and a windproof assembly, the adjusting base is rotationally installed at the bottom of the inoculating loop, an alcohol cotton groove with an opening in the top is formed between the adjusting base and the inoculating loop, the windproof assembly is vertically and slidably arranged in the alcohol cotton groove in a covering mode, abutting rods are symmetrically installed at the top of the adjusting base, and the abutting rods are matched with the windproof assembly in an abutting mode. The inner wall of the inoculating loop is fixedly connected with an arc-shaped track for the abutting rod to move, and an outlet is formed in one end of the arc-shaped track. By means of alcohol cotton burning in the alcohol cotton groove, the pollution risk in the inoculation process is effectively reduced, the sterile environment in the fermentation process is guaranteed, the inoculation operation is more flexible due to the design of the adjusting base and the arc-shaped track, the windproof assembly can be rapidly lifted by screwing the adjusting base, and the stability of flame burning is enhanced.

The utility model discloses an accurate quantitative inoculating loop for urine culture, which relates to the field of medical detection auxiliary tools and comprises a cylindrical grab handle, and a first stepped hole groove and a second stepped hole groove which are small in bottom and big in top are formed in the bottom of the grab handle along the height direction; the first stepped hole groove is composed of a first lower stepped hole and a first upper stepped hole, and the second stepped hole groove is composed of a second lower stepped hole and a second upper stepped hole. A marking piece and an inoculating ring piece which can move up and down are respectively arranged in the first stepped hole groove and the second stepped hole groove; a first through groove and a second through groove which are communicated with the first upper step hole and the second upper step hole are respectively formed in the outer wall of the grab handle; a first push plate and a second push plate are arranged on the outer wall of the grab handle, and one end of the first push plate and one end of the second push plate penetrate through the first through groove and the second through groove and are arranged at the upper ends of the scribing piece and the inoculating ring piece respectively; the inoculating loop is not easy to pollute, and the operation is simple; meanwhile, the authenticity and the accuracy of quantification of the inoculating loop are realized.

The utility model provides an inoculating loop heating bracket for a laboratory, which comprises a bracket body, and the bracket body comprises a linear connecting section, a conical section and a linear supporting section; one end of the linear connecting section sleeves the inlet of the heater, the other end of the linear connecting section is connected with the large-opening end of the conical section, and the small-opening end of the conical section is connected with the linear supporting section; a first hollowed-out groove is formed in the linear connecting section, a second hollowed-out groove is formed in the conical section, and the first hollowed-out groove is communicated with the second hollowed-out groove; a fixing groove matched with the size of the inoculation rod is formed in the middle position of the inner wall of the linear supporting section. According to the inoculation loop, manpower can be saved, two hands can be released, an operator can continue to inoculate samples in the high-temperature heating process of the inoculation loop, and the risk that the operator is scalded can be effectively reduced.

This utility model relates to an inoculation port, and more particularly to an inoculation port and a simple fermentation tank. It belongs to the technical field of edible mushroom fermentation equipment. An inoculation port, fixed to the bottleneck of a plastic bucket during use, includes an annular alcohol tank and a hollow cylindrical inner body with an outer diameter adapted to the inner diameter of the bottleneck. The annular alcohol tank is fitted around the outer periphery of the hollow cylindrical inner body, or integrally connected to the outer periphery of the hollow cylindrical inner body. This utility model's inoculation port ensures efficient sterilization of the inoculation ring while fully considering the usage scenarios of non-professional users such as farmers. It combines ease of operation and cost control, providing a practical technical solution for the promotion and application of small-scale edible mushroom cultivation technology.

The invention relates to the technical field of inoculating devices, in particular to an inoculating loop scribing device. Comprising a manipulator, a pipetting module, first pipetting gun heads, a culture dish and a storage rack, the clamping end of the manipulator clamps the pipetting module, the lower end of the pipetting module is connected with the first pipetting gun heads in a clamped mode, and the storage rack is used for storing the multiple first pipetting gun heads; the first pipette tip comprises a fixing part, a connecting part, filter cotton, a core rod and an inoculating loop, the fixing part is clamped with the pipette module, the filter cotton is arranged in the connecting part, one end of the core rod extends into the connecting part and is clamped with the connecting part, and the other end of the core rod is integrally connected with the inoculating loop; a first camera is arranged on the manipulator, the first camera is used for shooting a first image in real time, the first camera is electrically connected with a controller, and the controller processes the obtained first image and plans a movement route from the manipulator to a lineation starting position of a culture dish when the manipulator clamps a pipetting module provided with a first pipetting gun head; and the inoculation scribing efficiency is improved.

1. The name of the design product: inoculation loop (electrical cauterization type). 2. The use of the design product: when cultivating microorganisms, take out the microorganisms and put them into the culture medium. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view. 5. The top view is omitted because it is clearly expressed in other views.

The invention relates to the field of microbiological treatment, in particular to a metal inoculating loop capable of being electrified to test impedance, which comprises a metal working end used for contacting and staining a sample; the mounting part is connected with the metal working end; the control module is electrically connected with the metal working end; wherein the control module comprises a mode switching unit used for switching between a heating mode and a detection mode; the power driving module is used for applying current to the metal working end in the heating mode, so that the metal working end is heated to realize sterilization; the impedance detection module is used for applying a detection electric signal to the metal working end in the detection mode so as to detect the sample staining condition; and the control module is configured to judge the state of a sample stained by the metal working end according to the change of the electrical characteristic parameter relative to a reference value in a detection mode. The metal ring has the functions of heating sterilization and impedance detection at the same time, and whether the dipping amount of the inoculating loop reaches a preset standard or not is objectively judged.

This utility model provides an inoculation loop cooling device, relating to the field of inoculation loop cooling technology. It includes a support frame, a temperature detection component, a cooling component, clamps, and an indicator module. Several sets of clamps are arranged in a horizontal array, allowing the inoculation loop to be held in place. The cooling component includes a fan and a distribution air rod. The distribution air rod has air holes connected to the fan, with the air outlet direction along the length of the inoculation loop. This design, by providing air holes that blow air along the length of the inoculation loop, can cover most of the end of the inoculation loop, improving cooling efficiency. Furthermore, a temperature detection component is included to detect the temperature of the inoculation loop. When the temperature detection component detects that the temperature of the inoculation loop is lower than a set value, the indicator module switches to an indicating state, allowing the operator to easily determine whether the inoculation loop has been cooled completely, thus contributing to improved inoculation efficiency.

The invention relates to the technical field of white spirit processing, and particularly discloses a strain culture device for white spirit brewing, which comprises a separation inoculation mechanism, a strain mechanism, a culture dish mechanism and a controller, the strain mechanism is arranged on the left side of the separating and inoculating mechanism; the culture dish mechanism is arranged on the right side of the separating and inoculating mechanism; and the controller is arranged outside the separating and inoculating mechanism. Manual contact is avoided through whole-process automatic operation, the risk that infectious microbes are introduced into the environment is greatly reduced by combining a closed operation space and sterile airflow protection, flame forms are restrained, it is ensured that all parts of the inoculating loop can reach the high temperature sufficient for sterilization, the risk that a culture medium is polluted due to incomplete sterilization is effectively reduced, and the safety of the inoculating loop is improved. Meanwhile, rapid cooling can be achieved in the cooling link, secondary pollution in the cooling process is avoided, and the sterile effect of strain purification is remarkably improved.

The invention discloses a stoma urine culture device, and belongs to the technical field of culture equipment.The stoma urine culture device comprises a first circular shell, a second circular shell is fixedly arranged on the first circular shell, and a partitioning assembly and a calling assembly are arranged in the first circular shell. The problem of time superposition of single sample culture is avoided, a fan-shaped sliding plate I and a fan-shaped sliding plate II are in gapless fit with a storage chamber, and when a position adjusting sliding frame is jointed with a fan-shaped strip plate, the end part of an air guide pipe can be sealed, external air interference is isolated, a stable and pollution-free culture condition is provided for the growth of microorganisms in urine; the culture dish is simple in structure and convenient to operate, relative stability of the culture dish and the culture dish can be maintained under the action of a tension spring, sample leakage or non-uniform strain distribution in the culture process is effectively avoided, the opening and closing degree and opportunity of the dish cover can be accurately controlled by arranging a calling assembly, sufficient operation space is provided for the inoculation loop to inoculate a urine sample, and meanwhile infectious microbe pollution in the inoculation process is avoided.

The application discloses a flavoring and viscosity-reducing microbial agent and application thereof in reconstituted tobacco concentrate. The flavoring and viscosity-reducing microbial agent is prepared by the following method: (1) culture and enrichment of microbial bodies: Klebsiella sp. HNYJ-1 monoclone preserved in the China General Microbiological Culture Collection Center with a preservation number of CGMCC No. 24327 is inoculated into LB liquid culture medium by using an inoculating loop, and is cultured to obtain original seeds; and (2) the flavoring and viscosity-reducing microbial agent is obtained by gradient induction of the microbial bodies. The flavoring and viscosity-reducing microbial agent is used for preparing reconstituted tobacco concentrate with flavoring and viscosity reduction. After treatment of the reconstituted tobacco concentrate with the flavoring and viscosity-reducing microbial agent, the contents of protein, pectin and starch in the reconstituted tobacco concentrate are reduced, the fiber is basically unchanged, and the viscosity is reduced; meanwhile, the contents of alcohol, aldehyde ketone and ester flavoring substances in the reconstituted tobacco concentrate are obviously increased, the content of heterocyclic substances is increased, and the total amount of flavoring substances is increased.

This application provides a disposable inoculation loop ejection device. The device includes: a housing, an inoculation loop container, a spring-loaded assembly, and a central rod; a spring-loaded hole is formed on the top surface of the housing; the inoculation loop container is disposed inside the housing with its bottom surface inclined; the spring-loaded assembly includes a spring-loaded button, a metal rod, an inoculation loop clamp, and a spring; the spring-loaded button is located on the top surface of the housing; the metal rod is connected to the spring-loaded button and the inoculation loop clamp; the inoculation loop clamp passes through the inoculation loop container and has a gripping structure at one end, and is movably connected to the central rod at the other end; the spring is connected to the outside of the inoculation loop container and the inoculation loop clamp; the central rod is located at the bottom of the housing. When the spring-loaded button is pressed, the inoculation loop clamp moves downwards; when the spring-loaded button is released, the spring drives the inoculation loop clamp to reset, and simultaneously, the gripping structure of the inoculation loop clamps the disposable inoculation loop inside the inoculation loop container and ejects it through the spring-loaded hole. This disposable inoculation loop ejection device has a simple structure, is easy to operate, and avoids contamination.

The utility model discloses a novel disposable inoculating device which comprises an inoculating needle, an inoculating ring, a rolling shaft, a grab handle and a switching structure, the inoculating needle and the inoculating loop are both fixed on the rolling shaft; the rolling shaft is rotationally mounted on the grab handle; the switching structure acts on the rolling shaft to enable the rolling shaft to rotate; through the design of the switching structure, rapid switching of the inoculating needle and the inoculating ring is achieved, the operation process is simplified, the working efficiency is improved, the front end of the grab handle is designed to be in a sheet shape, installation and disassembly of the rolling shaft are convenient, and the rolling shaft, the inoculating needle and the inoculating ring can be used as disposable articles. The tedious process that inoculation components of a traditional inoculation loop need to be replaced frequently is avoided, and the long-term use cost is reduced.

The utility model discloses a kind of inoculation loop fixed support, it is related to microbiology experimental tool technical field, technical scheme is, including tripod, disc and the connecting rod of connecting tripod and disc, tripod includes three supporting legs;The bottom of each supporting leg of tripod is provided with antiskid rubber pad;The top of the three supporting legs of tripod is fixedly connected through the annular plate being set in middle, bottom connecting sleeve is set on the outer wall of annular plate;Disc edge evenly distributes semicircular groove.The beneficial effects of the utility model are: through the design of double inoculation loop rotation use's support, the time that operator waits inoculation loop cooling is reduced, and experimental efficiency is significantly improved;The design of multiple specifications of groove on disc can adapt to the handle of inoculation loop of different diameters, and the versatility of support is improved;The antiskid rubber pad of tripod and the stepped groove design of disc, ensure the stability of inoculation loop when placing, reduce the risk of sliding.

This invention discloses a method for systematically isolating and identifying Verticillium wilt of cotton, with the following steps: (1) disease sample collection and initial treatment; (2) strain culture: transfer the diseased tissue to a culture dish containing ampicillin PDA medium and culture it in a constant temperature incubator at 28°C for 3-5 days; (3) streak isolation and colony screening: place the culture dish under a microscope to directly observe the colony morphology. If the colony has the characteristic morphological features of Verticillium wilt of cotton, then the culture dish is confirmed to contain Verticillium wilt of cotton colonies; (4) single colony purification: from the culture dish containing Verticillium wilt of cotton colonies confirmed in step (3), pick a single colony with a sterile inoculation loop, transfer it to a new PDA medium for streak culture, and perform 2-3 consecutive passages for purification to obtain a purified strain; (5) DNA extraction and molecular identification; (6) pathogenicity verification inoculation; (7) re-isolation verification.

The utility model discloses an inoculating loop sterilization device, relates to the related field of inoculating loop sterilization, and aims to solve the problems that in the prior art, an inoculating loop cannot be positioned and fixed, so that only one inoculating loop can be sterilized at a time, the efficiency is low, and the sterilizing effect of the inoculating loop cannot be ensured. A support frame is arranged at the front end of the upper end of the infrared inoculating loop sterilizer body, the support frame comprises a first sterilization piece, a second sterilization piece and a third sterilization piece, the first sterilization piece, the second sterilization piece and the third sterilization piece are sequentially arranged, and each of the first sterilization piece, the second sterilization piece and the third sterilization piece comprises a fixing ring and an inclined arc-shaped support plate; the inclined arc-shaped supporting plate is fixed in the fixing ring and is obliquely arranged, the upper ends of the first sterilization part, the second sterilization part and the third sterilization part are all used for placing the inoculating loop body, and a loop body of the inoculating loop body is positioned in the infrared inoculating loop sterilizer body.

The utility model relates to based on the sterilization cooling inoculation integrated chamber of edible fungus of normal pressure steam sterilization mode, including the shoe changing room, the dressing room, the buffer room and a plurality of sterilization cooling inoculation area, the shoe changing room is connected with the dressing room, the dressing room is connected with the buffer room, and the buffer room is connected with a plurality of sterilization cooling inoculation area, the shoe changing room, the dressing room and the buffer room all set up ultraviolet lamp, and the dressing room and the buffer room set up fan filter unit and three -level filtration fresh air machine, and the buffer room sets up ozone machine, along the length direction of buffer room, and ozone machine and three -level filtration fresh air machine are arranged respectively in both ends of buffer room, and there is interval area between sterilization cooling inoculation area and buffer room, and the side of sterilization cooling inoculation area towards buffer room is provided with heat insulation wall, and ozone machine is communicated with a plurality of sterilization cooling inoculation area through pipeline, and three -level filtration fresh air machine is arranged in sterilization cooling inoculation area. The utility model keeps the clean of the sterilization cooling inoculation integrated chamber, reduces the fungus bag carrying work, and guarantees the fungus bag inoculation success rate.

The utility model discloses a disposable sterile sputum cup which comprises a spiral soft plastic cup, the outer surface of the spiral soft plastic cup is in threaded connection with a cup cover, a hollow small pipe is arranged on the upper surface of the cup cover, a plastic inoculating loop is arranged on the outer surface of the hollow small pipe, and a connecting pipe is arranged on the outer surface of the plastic inoculating loop. A top cover clamping seat is fixedly connected to the outer surface of the hollow small pipe, an inserting groove is formed in the outer surface of the top cover clamping seat, and a soft rubber protection cover is arranged on the inner surface of the inserting groove. By arranging the spiral soft plastic cup, the hollow small pipe, the plastic inoculating ring, the connecting pipe and the soft rubber protective cover, after a patient spits sputum and covers the cup cover tightly, the cup cover of the sputum cup does not need to be opened until inspection is completed, and only the soft rubber protective cover needs to be opened. Therefore, the biological safety problem that the environment is polluted due to the fact that sputum in a traditional sputum cup adheres to a cup cover and a sample is dropped on an operation table is solved, and the problem that sampling is difficult due to the fact that the sputum is not broken is solved.

The utility model discloses a high-flux single cell screening and purifying device which comprises a purifying mechanism, the purifying mechanism comprises a constant-temperature box, two guide sleeves installed on one side of the constant-temperature box, an experiment table connected between the two guide sleeves in a sliding mode, and two supporting legs movably connected with the bottom of the experiment table. According to the utility model, after the experiment table is horizontally placed, the supporting legs are opened, the experiment table is moved downwards to enable the supporting legs to be attached to the table top, then the constant temperature box is opened, a culture dish containing samples in the constant temperature box is directly placed on the experiment table for observation, the samples are qualified if the colors of the samples are white, other colors of the samples are not qualified, and then an alcohol lamp is used for performing over-fire sterilization on an inoculating loop to complete sterile treatment. The method comprises the following steps: firstly, preparing a culture dish, then dividing the culture dish into four areas by adopting a flat plate scribing method, completing inoculation, and finally putting the culture dish into a constant-temperature box for 1-2 days, so that repeated carrying of the culture dish is avoided and the working efficiency is remarkably improved due to the collaborative design of an experiment table and the constant-temperature box.