The invention provides a gonococcus transport and preservation method, which comprises the following steps: step 1, taking out one strain which is not freeze-dried from an ultralow-temperature refrigerator, placing the strain at
room temperature for later use, after the strain is slightly dissolved, taking out a certain amount of bacterial liquid from a
biosafety cabinet by using a pipettor, inoculating the bacterial liquid onto a pre-heated culture medium, marking lines in four regions, and then placing the culture medium in an
incubator with a
candle jar or a CO2
incubator at 36 DEG C for culturing for 36 hours; 2, picking pure
bacteria in a
biosafety cabinet by using a disposable inoculating loop, inoculating the pure
bacteria to a pre-heated culture medium, and placing the culture medium in a 36-DEG C
incubator with a
candle jar or a CO2 incubator for culturing for 24 hours; and step 3, taking one sterile glass
test tube, adding 1mL of sterilized normal
saline, scraping bacterial
lawn by using a disposable inoculating loop,
grinding along the tube wall, putting on a vortex device, sufficiently and uniformly mixing, and adding into sterilized skim milk. The strain is subjected to freeze-
drying preservation through a vacuum freeze-
drying technology, so that
energy expenditure for preservation of gonococcus is saved, and benefits are brought to distribution of national gonococcus interventricular
quality assessment substances and transportation of national gonococcus
drug-resistant monitoring point specimens.