79 results about "Inoculation loop" patented technology

The invention discloses a method for preparing a gamma-poly glutamic acid bioflocculant by bacillus licheniformis, relates to a bioflocculant, and provides a method for preparing the gamma-poly glutamic acid bioflocculant by the bacillus licheniformis. The method comprises the steps of transferring the bacillus licheniformis to a slant culture medium to cultivate; choosing an activated strain by an inoculating loop; inoculating into a conical flask with seed culture medium to cultivate; inoculating into the conical flask with seed culture medium to cultivate; taking a secondary seed, inoculating into a fermentation culture medium to cultivate by 3% of inoculating quantity; centrifuging bioflocculant fermentation liquor, removing the sediment, collecting upper clear liquid; adding ethanol to the upper clear liquid and then stewing, centrifuging and removing the upper clear liquid; dissolving the sediment into deionized water; dialyzing by a dialysis bag; adding absolute ethyl alcohol to the dialyzed solution, evenly agitating, centrifuging and removing the upper clear liquid; freezing and drying the sediment in vacuum to obtain pure gamma-poly glutamic acid bioflocculant. The method is short in fermentation cycle, high in activity of synthetic flocculant, good in thermal stability, and large in industrial application potential.

The invention belongs to the technical field of biology, and discloses an AFB1 degrading bacterium and an AFB1 degrading enzyme. The degrading bacterium is aspergillusoryzane AOYIN2012Y8 with a collection number of CGMCC No.5817. The aspergillusoryzane AOYIN2012Y8 CGMCC No.5817 is inoculated into a PDA solid culture medium, and constant-temperature culturing is carried out under a temperature of 28-30 DEG C; collection is carried out when a lot of spores grows. The spores are scraped off by using an inoculation loop. The spore concentration is regulated to 1.0*10<8>CFU / mL by using normal saline containing 0.5-0.7% Tween 80. 5-7mL of the spore is inoculated to every 30g of a solid fermentation culture medium. Constant-temperature culturing is carried out for 5-7 days under a temperature of 28-30 DEG C, and collection is carried out. The solid fermentation culture medium is well mixed with normal saline, and the mixture is subjected to room-temperature soaking. The mixture is filtered and centrifuged; a supernatant is fetched and is subjected to salting-out, dialysis, and gel chromatographic separation; and concentrating and lyophilizing are carried out, such that the AFB1 degrading enzyme is obtained. The degrading enzyme provided by the invention has relatively high decomposing capability against AFB1, wherein a maximal degradation rate reaches 80.12%.

Disclosed is a device for inoculating mushroom and black fungus sticks. The device comprises a fungus stick hole punching chamber, an inoculation chamber and a film fungus stick chamber. In the inoculation process, the processes of manual fungus stick placing, mechanical stick pushing, mechanical hole punching, manual strain filling and manual bagging are adopted, and the advantages of being not high in investment and clear in work division of operation personnel, saving labor, being high in efficiency, controllable in inoculation environment, less in damage to finished strain blocks, high in inoculation yield and stable, achieving continuous operation and the like are achieved. The device is an inoculation machine integrating the advantages of an inoculation box, an open inoculation machine and a full-automatic inoculation machine, is suitable for fungus stick type hole punching inoculation of mushrooms, black fungus and other edible mushrooms, and can be used under the condition of greenhouses, common room interiors, clean rooms and other various environments.

The invention discloses a preparation method for kitasamycin industrial production strains, which comprises the main steps: performing plate streaking on an original strain to prepare a single colony; transferring the single colony onto an inclined plane, culturing same for a proper time; then scraping spores on the inclined plane with an inoculation loop, and preparing a freeze-dried strain tube with a vacuum freeze-drying method; preparing a spore suspension by using the freeze-dried strain tube, coating the oblique plane with the spore suspension, culturing the spore suspension for a proper time; then scraping spores on the oblique plane with the inoculation loop to prepare a spore and milk suspension; subpackaging the spore and milk suspension and performing freezing preservation so as to prepare a spore and milk freezing tube; melting the spore and milk freezing tube, and transferring same to a seed culture medium, performing shake culture by bottle shaking for a proper time, and then transferring to a seed tank. The method overcome the defects of poor synchronization of inoculation production of inclined strain production, nonuniform growth cycle of seed bottles and frequency of batch number change. The preparation method disclosed by the invention is simple and convenient to operate, the inoculum age of strains and consistency of quality of seeds are improved, the batch number change of seeds is reduced, and the preparation method is very suitable for kitasamycin industrial production.

The invention discloses a method for preparing rhodovulum sulfidophilus liquid. The method includes the following steps: (1) a cryopreserved tube containing the rhodovulum sulfidophilus liquid is dissolved and revived, the dissolved bacteria liquid comes to be revived bacteria liquid; (2) on an ultra-clean working platform, by utilization of the revived bacteria liquid, streaking is carried out on a flat plate solid medium, the streaked flat plate solid medium is placed in a constant temperature incubator with the temperature of 30 DEG C for being cultivated for 24-36 hours in a static mode, after single colonies are grown, an inoculating loop is utilized to pick the single colonies on the flat plate solid medium to streak on a slope culture medium, the streaked slope culture medium is placed in the incubator with the temperature of 30 DEG C for being cultivated for 24-36 hours in a static mode, and bacterial colonies are grown; and (3) after the bacterial colonies are grown, by utilization of a rhodovulum sulfidophilus liquid culture medium, the bacterial colonies on the slope culture medium are eluted to be bacterial colony liquid, the bacterial colony liquid is cultivated for the first time so as to obtain first-time culture bacterial liquid, and transfer culture is carried out on the first-time bacterial liquid to obtain transfer culture bacterial liquid. The method for preparing the rhodovulum sulfidophilus liquid has the advantages of being easy in preparation and culture of the culture media, high in the bacteria liquid concentration and good in quality.

The invention relates to a microbial enzymes racemization preparation method of DL-alanine, comprising the following steps: preparing a substrate, adjusting the pH value of the substrate to eight by ammonia or sulphuric acid, canning the obtained substrate in flasks, inoculating microorganisms saved on an inclined plane to the substrate by picking one loop of microorganisms with a transfering loop, culturing the substrate for 24h at 30 DEG C by a rotary shaker of which the rotation rate is 110rpm, merging the above cell supernatant, adding the cell supernatant in a substrate solution, reacting at 30 DEG C at pH value of 7.0 when a polarimeter is used to measure the specific rotation of the reaction system every hour, continuing to react when the specific rotation is zero and heating up to 60-100 DEG C, destaining for 30min by adding active carbon, filtering, and vacuum concentrating and crystallizing by a rotary film evaporator to obtain the DL-alanine finished goods. The invention has the advantages of accessible raw materials, simple production process and mild conditions, and can greatly reduce the production cost of DL-alanine.

The invention discloses a method for improving yield of fruiting bodies of cordyceps takaomontana. The method comprises the steps of activating an ustilago esculenta GZUIFR-DX091 bacterial strain by applying a PDA culture medium, picking three loops for the activated bacterial strain by applying an inoculating loop to access into the ustilago esculenta culture medium, centrifuging culture liquor for 5 minutes at 5000r / min after culturing for 5 days at 28 DEG C at 160r / min, and getting liquid supernatant to obtain fermentation liquor of the ustilago esculenta GZUIFR-DX091 bacterial strain; infecting silkworms with cordyceps takaomontana, and starting producing fruiting bodies for later use when the infected silkworms are hardened; pre-sterilizing a culture container, and tiling single layer or double layers of sterilized water-absorbing paper at the bottom of the container; slowly adding the prepared fermentation liquor of the ustilago esculenta GZUIFR-DX091 bacterial strain onto the water-absorbing paper at the bottom of the container, placing the infected silkworms into a container which is added with the cane shoot fermentation liquor, and carrying out culture under light for 12 hours at 20-23 DEG C, wherein the culture temperature can be lowered by 5 DEG C for culturing at a dark culture stage. In the culture process, if the added fermentation liquor is dried, the fermentation liquor is needed to be timely complemented; the fruiting bodies of cordyceps takaomontana can be harvested after culturing for 3-4 weeks. The method disclosed by the invention can greatly improve the yield of the fruiting bodies of cordyceps takaomontana.

The invention discloses a method for quickening the colonization of bag-cultured Lypphyllum decastes mycelium, comprising the steps of: formulation of culture medium of cultivar, bagging, inoculation, cultivation, peeling off, bud promotion, development and collection, wherein the inoculation step is carried out in case that a fan filter unit capable of efficient filtration is mounted, the ambient temperature for inoculation is 20 DEG C, the inoculation quantity is 30g / per bag, and the surface of bagged culture material is covered by the strain as widely as possible during inoculation.

The invention relates to a method for measuring the inhibition zone of yellow rice wine by a filter paper dispersion method, which comprises the following steps of: (1) bacterial solution preparation: inoculating escherichia coli and staphylococcus aureus strains on the inclined plane of a beef extract-peptone culture medium, picking bacteria from the inclined plane to 100 mL of sterile water by an inoculating loop to prepare the bacterial solution, and plugging by tampon for later use; (2) preparation and treatment of filter paper: punching round filter paper by a puncher, performing dry heat sterilization for later use, clamping the sterilized filter paper by forceps and soaking the filter paper into medicine liquid and wine samples with different concentrations for later use; and (3) inoculation and cultivation: preparing a flat plate by the sterilized beef extract-peptone culture medium under the aseptic condition, adding bacteria for testing after cooling and solidification, coating uniformly by an aseptic coating device, clamping the filter paper, which is subjected to extraction treatment, by aseptic forceps, and adhering the filter paper to a bacterium-containing plate. By the method, the inhibition effect of mildew and bacteria can be controlled, so that a theoretical basis and a technical support are provided for production, research and development of the functional yellow rice wine.

The invention discloses a novel intelligent automatic separation device for bacterial examination, which belongs to the technical field of bacterial examination separation. The device comprises an uninoculated culture dish storage box, a bacterial separation inoculation work box and an inoculated culture dish storage box, and a cross mechanical arm and a manipulator are arranged on an operation platform in the bacterial separation inoculation work box. Transportation of the culture dish, opening and closing of a culture dish cover, replacement of an inoculation ring, sample loading of a detection sample, automatic bacterium separation and streak are completed automatically in a programmed mode, streak separation of bacteria on a culture medium is completed, and under the condition of no artificial participation, a computer controls a machine in a closed space to complete bacterium separation and streak. The workload of personnel can be reduced, the bacteria separation process is finer,sample and environment pollution is reduced, and the personnel infection risk is prevented.

The invention discloses a production method of ultra-low water absorption bamboo wood fiber decorative plate. (1) fermentation broth is prepared, specifically, a bacillus subtilis strain xp is suspended in an LB liquid medium, and an inoculating loop is used for picking up a bacterial solution and culturing on a slant at 20-40 DEG C for 2-14 h; bacterium obtained are aseptically picked up a singlecolony by the inoculation loop and the single colony is inoculated into an LB liquid medium containing 0.1 g / L malic acid and cultured in a shaking table at 30-38 DEG C until an OD600 value is 2.0, and the fermentation broth is obtained; (2) raw materials prepared, specifically, the raw materials are all dried to a moisture content of 8% and comprise, by weight, 50-55 parts of bamboo fibers and 5-8 parts of pine wood shavings, and the raw materials are uniformly mixed; evenly mixed raw material plant fibers are uniformly sprayed with the fermentation broth and turned, and the mixture is fermented at 32-38 DEG C for 8-10h; the amount of spray is that the fermentation broth is 0.001 time the weight of the plant fibers; after fermentation, drying is carried out at the temperature of 60 DEG Cto a moisture content of 8%, and the raw material plant fibers are pulverized into powder for use; (3) molding is performed.

The invention discloses a disposable specimen collecting and inoculating device. The disposable specimen collecting and inoculating device comprises a collecting container and a sealing cover used for sealing the container, wherein an inner sleeve is fixed in the center of the inner side surface of the sealing cover; the bottom of the inner sleeve is fixed with an inoculating ring and a cotton swab capable of moving up and down in the sleeve; a limiting bracket for limiting position of the cotton swab is arranged on the tail part of the cotton swab; a clamping ring which is matched with the limiting bracket and plays a position limiting role is arranged on the inner wall of the hollow tube; the tail end of the limiting bracket is connected with a traction wire which is used for pulling the limiting bracket to cross the limiting ring for enabling the cotton swab to be retracted into the hollow tube; after penetrating through the sealing cover, the traction wire is connected with a pulling ring used for traction operation. According to the disposable specimen collecting and inoculating device disclosed by the invention, the specimen collecting, inoculating and drawing are completed by one step, and the disposable specimen collecting and inoculating device is discarded after being used, so that a process of heating the inoculating ring on an alcohol lamp till red to realize sterilizing is cancelled, and therefore, not only is crossed pollution avoided, but also time is saved and working efficiency is improved.

The invention discloses an inoculator for common cassava mosaic viruses, belongs to the field of plant pathogen inoculation tools, and aims to overcome the defect that manual inoculation wastes time and labor in the prior art. The inoculator comprises a shell attached to cassava branches and a cutter sliding on the surface of the shell. The surface of the shell is provided with through holes whichare arranged in lines. The cutter is provided with a sliding sleeve attached to the shell. The cross section of the sliding sleeve is a split cylinder; a cutting blade is arranged at the head end ofthe sliding sleeve; a pressing plate is fixedly connected to a cutter handle of the cutting blade, a telescopic piece serving as a pressing plate fulcrum is connected to the bottom of the pressing plate, and the telescopic piece is in a compressed state when not passing through the through hole. The pressing plate and one end of the cutting blade descend when the telescopic piece is straightened,the movement stroke of the pressing plate is located outside the sliding sleeve, and a rotating shaft passes through the pressing plate close to the cutting blade. According to the inoculator, the cutting function of the inoculation link is achieved, manual cutting is avoided, the generated torque is provided by the telescopic piece, and the strength of an operator is saved.

The invention discloses a ring burning device. The ring burning device comprises a supporting frame, a cooling base, a clamp assembly, a heating element, a sensing element, a timer and a controller. The cooling base, the clamp assembly and the heating element are sequentially fixed to the supporting frame in the same vertical direction from bottom to top. The sensing element is arranged on the supporting frame and / or the clamp assembly. The cooling base is provided with a containing cavity with an opening in the top, the containing cavity is located under the clamp assembly, the sensing element, the clamp assembly, and the heating element and the timer are all in data communication with the controller. The invention further discloses ring burning equipment comprising the ring burning device. According to the ring burning device and the ring burning equipment, the sterilization time of an inoculating loop can be controlled, automatic cooling is achieved after sterilization, transferringis not needed, and careless mistakes are not likely to happen.

The invention discloses a method for controlling inoculation force of solid culture media by the aid of damping force and sensor technologies. The method is implemented by the aid of an inoculation loop damping and sensor detection assembly, an inoculation loop rotary driven shaft, an inoculation loop rotary driven wheel, a synchronous belt, an inoculation loop rotary motor mounting component, an inoculation loop rotary driving wheel, an inoculation loop rotary motor, an inoculation loop rotary component, a switching component, a Z-axis slide table module, a base plate, a culture dish and the culture media. The inoculation loop damping and sensor detection assembly comprises an inoculation loop mounting component, and two inoculation loop mechanisms are arranged on the inoculation loop mounting component and comprise inoculation loops, inoculation loop switching components a, inoculation loop switching components b, inoculation damping springs and inoculation sensing adjusting screws. The method has the advantages that force applied to the surfaces of the culture media when the inoculation loops are in contact with the surfaces of the culture media in a pressure manner can be guaranteed; the inoculation loops can be assuredly in contact with the surfaces of the culture media in inoculation procedures; the service cost can be reduced to a great extent; poor scribing effects because of jumping of the inoculation loops due to high-speed scribing can be prevented.

The invention relates to a microorganism sample pretreatment system which comprises a workbench, a sampling groove and a through groove are formed in the workbench and located on the two sides of a sterilization equipment respectively, and the connecting line of the center of the sampling groove and the center of the sterilization equipment is perpendicular to the length direction of the through groove and passes through the midpoint of the length of the through groove. A clamping mechanism capable of controlling the clamping of the sample tube is arranged above the sampling groove; the scribing equipment comprises an XZ double-axis linear module, an inoculating loop is installed on a sliding block of the Z-axis linear module, and a suction cup driven by a Y-axis linear module is arranged below the workbench. The untreated culture dish bin comprises a top plate and a bottom plate which are fixed through connecting columns, two supporting plates are fixed to the positions, below each arc-shaped lower notch, of the bottom plate through connecting plates, and the faces, connected with the arc-shaped lower notches and the supporting plates, of the connecting plates are arranged to be arc-shaped slopes. According to the invention, full-mechanical operation is utilized to simulate manual microorganism separation, so that risks of infection and inaccurate results during manual operation can be reduced, the working efficiency is improved, and the labor cost is reduced.

The invention discloses an inoculation loop rod with self-disinfection function. The inoculation loop rod includes an inoculation rod, the inoculation rod is internally equipped with a storage battery chamber and a heating wire chamber, the heating wire chamber is internally provided with a heating wire ring, two ends of the heating wire ring extend to the outside of the heating wire chamber bottom, both ends of the heating wire ring are connected to an inoculation loop, the storage battery chamber is internally equipped with a storage battery, and two ends of the heating wire ring are respectively connected to the positive electrode and negative electrode of the storage battery. The inoculating loop disclosed by the invention can be cooled under ultraviolet lamp irradiation after high temperature disinfection, asepsis and an appropriate temperature can be ensured to the inoculating loop, death of strains from scald due to high temperature of the inoculating loop can be avoided, and the success rate of inoculation is increased.

The invention discloses a method for improving the yield of astaxanthin in a wild phaffia rhodozyma strain by using an inorganic-microorganism hybrid system. The method comprises the following steps of: shake flask seed culture: taking an inoculating loop strain from an original slant strain slant of phaffia rhodozyma, inoculating a seed culture medium with the inoculating loop strain, and performing shake flask culture at 22 + / -5 DEG C and at the rotating speed of 180-240 rpm for 24-72 hours to obtain a seed culture solution; and shake flask fermentation culture: inoculating a carbon nitride nanosheet fermentation medium with the concentration of 0.2-6 g / L with the seed culture solution according to the volume ratio of 10%, and culturing a shake flask at the temperature of 22 + / -5 DEG C and the rotating speed of 180-240 rpm for 48-108 h, wherein the fermentation culture process is performed under the condition that the whole process is light or completely dark. According to the method, the yield of the astaxanthin of the phaffia rhodozyma can be remarkably increased by a method for assembling a hybrid system by using light-driven carbon nitride nanosheets and the phaffia rhodozyma.

The invention relates to an automatic scribing equipment and a scribing method for a microorganism sample pretreatment system. The automatic scribing equipment comprises an inoculation table, an XZ double-axis linear module is installed above the inoculation table, an inoculation ring capable of controlling rotation is installed on a sliding block of the Z-axis linear module, a Y-axis linear module is installed below the inoculation table, and a Y-axis linear module is installed below the Y-axis linear module. A through groove is formed in the inoculation table and located over the Y-axis linear module, a suction cup capable of moving in the through groove is installed on a sliding block of the Y-axis linear module, and the suction cup is connected with a vacuum generator through an air pipe. The scribing equipment is diverse in scribing form, high in scribing efficiency and high in automation degree.

The invention relates to a proliferation method and an application of small selective-breeding vitamin C two-step fermentation bacteria. The proliferation method comprises the following steps of: diluting small bacteria treated with a physical or chemical method; coating the small bacteria onto a flat plate and culturing for 2 to 3 days; picking single colonies of the small bacteria one by one through inoculating loops; separately coating the single colonies onto the separated flat plate by using a scoring method; picking big bacteria by using the inoculating loops, uniformly inoculating the big bacteria into the flat plate on which the small bacteria are coated and carrying out enrichment culture for 2 to 3 days; inoculating the proliferated small bacteria onto a cant one by one; picking the big bacteria by utilizing inoculating needles, inoculating the big bacterial into the cant on which the small bacteria are coated and culturing the big bacteria; inoculating two loops of cant bacteria mosses which grow well into a seed culture medium and culturing for 16 hours; transferring the cant bacteria mosses into a culture fermentation medium according to 10% of inoculum size and culturing for 3 to 4 days; detecting the content of Keto-L-gulonic acid in a fermentation liquor and the concentration of residual L-sorbose; and selecting target strains according to the statistical analysis of experimental data.

The invention discloses an intelligent adjustable self-heating inoculator for microbial experiments. The inoculator comprises a head inoculating loop, a sliding rod and a handle which are connected in sequence, wherein the head inoculating loop comprises an inoculating needle and two spliced inoculating loops; the inoculating needle is mounted at the top of the sliding rod; a sliding block track and two sliding blocks are arranged in the sliding rod; the bottoms of the spliced inoculating loops are connected with the sliding blocks; a gear shifting mechanism comprises a push button, a guide rail sliding block and two connecting rods; the guide rail sliding block is arranged at the handle; the push button is assembled on the guide rail sliding block in a sliding manner; the handle comprises a constant-temperature heating device and a temperature controller which are connected with a battery through wires; the constant-temperature heating device is used for heating and adjusting the temperature of the head inoculating loop; and the temperature controller electrically connected with the constant-temperature heating device is used for monitoring the environment temperature and the overall temperature of the head inoculating loop. The inoculator is simple to operate and low in cost, and can meet the requirements of various microbial experiments.

The invention belongs to the technical field of mechanical supporting, particularly relates to an integrated bacteria-proliferating sampling tube for anal examination, and further provides an integrated bacteria-proliferating sampling method for the anal examination and an integrated bacteria-proliferating sampling application for the anal examination. The integrated bacteria-proliferating sampling tube for anal examination comprises a sampling stick and an inoculating loop which are of an integrated structure, and the sampling stick and the inoculating loop are communicated with each other. The problem of environmental pollution caused by the fact that the sampling stick is required to be pulled out during sampling by the inoculating loop, the pulled-out sampling stick is difficult to place and a liquid on the sampling stick is easy to drop in the prior art is solved, and the technical beneficial effects of effectively preventing environmental pollution, convenient collection and disinfection and compact structure are achieved.

The invention discloses an inoculation device for microbiological assay. The inoculation device comprises a device base, wherein a lifting and transverse moving unit is arranged on the end face of theupper side of the device base and is used for providing lifting motion and transverse motion for inoculation; an inoculation unit is arranged on the lifting and transverse moving unit; the inoculation unit is used for taking seeds and conduction inoculating; the inoculation unit can automatically control a contact force with a culture medium so as to protect the culture medium from being scratched; a fixed frame positioned on the left side of the inoculation unit is fixedly connected to the end surface of the upper side of the device base; a fixed sterilization unit is arranged in the fixed frame; the fixed sterilization unit is used for high-temperature disinfection and cooling of an inoculation ring; and a sealing plug taking and covering unit is arranged on the end surface of the upperside of the device base and located at the right side of the fixed sterilization unit. The inoculation device of the invention can replace manual work in inoculation operation with a slope inoculation method, and improves inoculation efficiency, and inoculation quality can be easily ensured by using mechanical inoculation.

The invention discloses a wild mulberry phellinus igniarius strain separation and purification method, and relates to the technical field of phellinus igniarius culture, phellinus igniarius has the advantages of high growth speed and short separation period, and the specific scheme is as follows: the method comprises the following steps: S1, performing surface disinfection on phellinus igniarius sporocarp under a sterile condition; S2, cutting off fungus blocks from the phellinus igniarius sporocarp and transplanting to a plate culture medium to be cultured under the dark condition; S3, observing the growth condition of phellinus igniarius, picking bright yellow or deep yellow hyphae through an inoculating loop when the bright yellow or deep yellow hyphae are found, separating on a new culture medium by adopting a four-region plate streaking method, and continuously culturing; S4, judging when infectious microbes appear, and repeating the step S3 when the infectious microbes affect the growth of the phellinus igniarius; when the infectious microbes do not affect the growth of the phellinus igniarius, the infectious microbes are removed under the sterile condition until purified phellinus igniarius is obtained; compared with a traditional broad-spectrum culture medium, the culture medium provided by the invention is not prone to infectious microbe infection and can resist infectious microbe.

The invention discloses a method for screening PHA producing bacteria. The method comprises the following steps: 1) manufacturing a culture medium; 2) enriching PHA producing bacteria; and 3) screening the PHA producing bacteria: spraying a bromothymol blue indicator into a culture medium plate, marking bluing bacterial colonies around the bacterial colonies, placing the bacterial colonies under an ultraviolet lamp for observation, and determining that the bacterial colonies are PHA producing bacteria if orange fluorescence appears; and picking the single colonies by using a sterilization gun head, performing smearing, placing the single colonies under blue light of a fluorescence microscope for observation and verification, then, picking the single colonies, performing oscillation and enrichment in a shaking table for 2 hours, dipping a small amount of bacterial liquid by using an inoculating loop for line drawing separation on a new plate culture medium, after inverse culture at 37 DEG C for 24 hours, picking the single colonies and performing numbering, and performing repeated purification and separation in such a way. According to the method, two indicators are selected from numerous indicators for screening PHA producing bacteria, and a one-step screening method is adopted, so that time and materials are saved; meanwhile, the false positive rate can be reduced; and the period required by purification and separation can be shortened.

The invention relates to a microbe separation and identification lineation marker which effectively solves the problems that manual operation is needed in the existing microbe lineation marking process, cross infection is prone to occurring, rapid linkage of the lineation marking process cannot be achieved, and the operation efficiency cannot be improved. According to the technical scheme, the device comprises a base, an alcohol lamp and a shell, a rotating column is coaxially and rotatably connected to the upper end of the base, mounting fixing sleeves are fixedly connected to the left side and the right side of the rotating column, sliding limiting sleeves are coaxially and slidably connected into the mounting fixing sleeves, and inoculating rings are detachably connected into the sliding limiting sleeves; two hydraulic cylinders communicating with the mounting fixing sleeves are arranged in the rotating column, lifting threaded sleeves are connected into the hydraulic cylinders in an up-down sliding mode, adjusting lead screws are coaxially connected to the lifting threaded sleeves in a threaded mode, and limiting shaft rods are connected into the adjusting lead screws in an up-down sliding mode; and the two limiting shaft rods are coaxially and fixedly connected with a large belt wheel and a small belt wheel which are connected through a belt respectively, and the limiting shaft rods are externally connected with a driving source. The structure is simple and practicability is high.

The invention relates to the technical field of biodegradation, and discloses a biodegradation method for zearalenone toxin in DDGS (Distillers Dried Grains with Solubles). The biodegradation method comprises the following steps of: in a super clean workbench, placing a collected soil sample in sterile distilled water to be oscillated for 15 minutes to prepare a bacterial suspension, keeping the revolving speed of a table concentrator at 200 rpm, after the bacterial suspension is subjected to concentration gradient dilution by the sterile distilled water, coating a NA culture medium flat platewith the bacterial suspension, culturing for 24 hours under a condition of 30 DEG C, enabling a bacterial colony to be spread on the whole flat plate, picking bacterial strains with different forms,sizes, colors and transparency by an inoculating loop to carry out lineation purification, carrying out spot inoculation on the purified the bacterial strains, and applying the purified the bacterialstrains to a zearalenone degradation experiment to obtain pseudomonas aeruginosa. According to a characteristic that the pseudomonas aeruginosa and aspergillus niger can be used for degrading the zearalenone toxin, the pseudomonas aeruginosa and the aspergillus niger are mutually cooperated to efficiently degrade the content of the zearalenone toxin in a DDGS feed product, so that the quality of the DDGS feed product is improved, safety is high, and no pollution is caused so as to be favorable for large-scale utilization.

The invention discloses a microbial preparation foam drainage gas production process which comprises the following steps: preparing diluents with different concentrations, inoculating thalli onto the diluents by using an inoculating loop, and then preserving at constant temperature to complete microbial culture operation; the cultivated microorganisms are injected into the stratum of the natural gas well, and meanwhile a foam scrubbing agent is injected into an oil pipe through foam scrubbing agent injection equipment; the method has the beneficial effects that microorganisms cultivated in different environments are injected into the stratum of the natural gas well at the same time, a certain amount of foam scrubbing agent is injected into the oil pipe to be matched with the oil pipe, the purpose of fast foam scrubbing gas production can be achieved, and the purpose of fast foam scrubbing gas production can be achieved; moreover, the microorganisms can generate a biosurfactant and various secondary metabolites in the gas well, a good cleaning effect is achieved on the near-wellbore zone of the well bottom, and the metabolites are protein, so that the environment is not polluted, and the pipeline flow is not corroded.

The invention discloses a lactic acid bacteria separating, screening and culturing device, and relates to the technical field of lactic acid bacteria separating and culturing. The lactic acid bacteriaseparating, screening and culturing device comprises a constant-temperature box, supporting bases are arranged at the bottom of the constant-temperature box, the multiple supporting bases are arranged, a disinfection box is fixedly connected to the top of the constant-temperature box, supporting plates are connected between the inner walls of two sides of the constant-temperature box in a slidingmode, culture dishes are arranged at the tops of the supporting plates, the number of the support plate and the culture dish are multiple, and a first clamping plate and a second clamping plate are correspondingly and fixedly connected between the inner walls, close to the top, of two sides of the disinfection box. According to the device, by arranging the disinfection box, rapid disinfection ofa inoculating loop is realized, the defect that the inoculating loop needs to be disinfected by an alcohol lamp every time the inoculating loop is used during streak separation of lactic acid bacteriaby a plate streak method is avoided, and the inoculating loop is placed in the disinfection box and can be directly taken out for use during use.